LOCUS       AP026637               11816 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA290 plasmid pAA290_4 DNA, complete sequence.
ACCESSION   AP026637
VERSION     AP026637.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466812
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11816)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..11816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA290"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA290_4"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
     gene            complement(465..1223)
                     /locus_tag="EfmAA290_34340"
     CDS             complement(465..1223)
                     /locus_tag="EfmAA290_34340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117494.1"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="BDP82758.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNMNTMEN"
                     /name="RepB family plasmid replication initiator protein"
     gene            complement(2075..2764)
                     /locus_tag="EfmAA290_34350"
     CDS             complement(2075..2764)
                     /locus_tag="EfmAA290_34350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82759.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(3004..3918)
                     /gene="mobA_1"
                     /locus_tag="EfmAA290_34360"
     CDS             complement(3004..3918)
                     /gene="mobA_1"
                     /locus_tag="EfmAA290_34360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDP82760.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="mobA_1"
     gene            complement(3900..4289)
                     /gene="mobC_1"
                     /locus_tag="EfmAA290_34370"
     CDS             complement(3900..4289)
                     /gene="mobC_1"
                     /locus_tag="EfmAA290_34370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDP82761.1"
                     /translation="MGLMSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_1"
     gene            complement(4582..5025)
                     /locus_tag="EfmAA290_34380"
     CDS             complement(4582..5025)
                     /locus_tag="EfmAA290_34380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82762.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKILQKYRTLVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            complement(4998..5375)
                     /locus_tag="EfmAA290_34390"
     CDS             complement(4998..5375)
                     /locus_tag="EfmAA290_34390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82763.1"
                     /translation="MHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLSE
                     VQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANA
                     GPVTIPVPGGGFTWDNTITNW"
                     /name="hypothetical protein"
     gene            complement(5500..6093)
                     /locus_tag="EfmAA290_34400"
     CDS             complement(5500..6093)
                     /locus_tag="EfmAA290_34400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82764.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTLKLRLNIFIPSKKNT
                     NGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGV
                     SSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVK"
                     /name="hypothetical protein"
     gene            complement(7280..7450)
                     /locus_tag="EfmAA290_34410"
     CDS             complement(7280..7450)
                     /locus_tag="EfmAA290_34410"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82765.1"
                     /translation="MKNRSFAILVLSVILLDILLITLALKTLNLVTSFVIIPVIIGMSI
                     YFIKKINNFFE"
                     /name="hypothetical protein"
     gene            7796..8194
                     /locus_tag="EfmAA290_34420"
     CDS             7796..8194
                     /locus_tag="EfmAA290_34420"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287522.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP82766.1"
                     /translation="MELDTNSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISESY
                     HITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMFWS
                     KSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="transposase"
     gene            8320..9357
                     /locus_tag="EfmAA290_34430"
     CDS             8320..9357
                     /locus_tag="EfmAA290_34430"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287525.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP82767.1"
                     /translation="MLDDRKKAYEEVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALAN
                     AQLNLDKAYKNFFRDKSVGFPRFKSKKNPVQSYTTNNQNGTVALIDIKVPKLKSLVRIK
                     LHRQPKGIIKSATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQK
                     IDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQR
                     TDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGR
                     KIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLH
                     SMGLA"
                     /name="transposase"
     gene            complement(9498..9683)
                     /locus_tag="EfmAA290_34440"
     CDS             complement(9498..9683)
                     /locus_tag="EfmAA290_34440"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82768.1"
                     /translation="MKKTKLLVASLCLFSSLLAFTPSVSFSQNGGVVEAAAQRGYIYKK
                     YPKGAKVPNKGELLTT"
                     /name="hypothetical protein"
     gene            complement(9773..10234)
                     /locus_tag="EfmAA290_34450"
     CDS             complement(9773..10234)
                     /locus_tag="EfmAA290_34450"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 10280;
                     possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82769.1"
                     /translation="MNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVRESDM
                     IFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHVFENI
                     GASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="hypothetical protein"
     gene            complement(10251..10433)
                     /locus_tag="EfmAA290_34460"
     CDS             complement(10251..10433)
                     /locus_tag="EfmAA290_34460"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82770.1"
                     /translation="MLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTG
                     RDFLLKIGRISRLSV"
                     /name="hypothetical protein"
     gene            complement(11326..11538)
                     /locus_tag="EfmAA290_34470"
     CDS             complement(11326..11538)
                     /locus_tag="EfmAA290_34470"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82771.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKNISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKKLW"
                     /name="hypothetical protein"
     gene            complement(11607..11789)
                     /locus_tag="EfmAA290_34480"
     CDS             complement(11607..11789)
                     /locus_tag="EfmAA290_34480"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP82772.1"
                     /translation="MQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKE
                     NNNAITTEKKWWKFW"
                     /name="hypothetical protein"
     Typing          complement(293..1223)
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.893"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3008..3918)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtatcttttt cttggagctg ttcatgtaaa aaaacaataa tcgagttttc atcgttacta
       61 gcaacggttt ctacttggtt accagtggtg ttactaccta aaaaatgttg ttttaaaagc
      121 aaatatcctt tgtagctgac aactaaggtt ttcactccgt tcctagtaac agtttgcaca
      181 tagttttctc taaaatcagc tgatagcctt ttcctaattg cttgttttga aacatgaaat
      241 tcttccgcga gttctttgat cgttttatct atcataaata ctccttatgc ttcaatcgaa
      301 taagttttgt aagttttcta ataaatcttg tttaagctca tttgagattg attgtttttc
      361 cgcttcttct ttttcggttt gttgttgttt tttaaaatca gcttcatgag tacctaacgg
      421 aagatttagt attcgatctt tagcacgcca tttttctttt tgtgttaatt ctccattgtg
      481 ttcatattaa ataattttat acgtaaatct tcttgcttcc cttttgaaaa atcgtcagca
      541 tggttgattt cagctttcca agtaaaacga taaccaataa caggtttacc tcgaccttta
      601 ccatattttt tacgaatagt taatccttta aatagtggag ttagttcttc ttttattggt
      661 ttgagtacaa actgatttat atttgtttgt ctatagcttt taggaatatc taatagctct
      721 aaaaaatctt ccttagaaaa ttcagcccat ccttgagttc ggtattgttt tagcaatcta
      781 aacattgttt tagcataact gctatttaaa tctcgaaatt ctgttaaaga gaaacgtacc
      841 cagctttcta gattattaag taaaggcaaa gctttttcgt ggacttttat atctacataa
      901 gggttatcga cttctccatg aatttcaaaa tcagtaaaca ttgtaaatcg ttctattgtt
      961 aatccaccat tgctttttct tccgaaacgt aaatccatca aatgatcgta tgttcttgcc
     1021 aaatctttta taaagacttc tcctgcagtc tgcttatacg cacttaaatc tttaagttgg
     1081 ctgaaataaa agcgtatttt ttgattacct ttatcgcgca ttctagagac aatagaaaaa
     1141 aacaagttca tttctattgg ggtaaatttt cttaaaggaa tggtatttaa ttctggttca
     1201 tactttacga tttcatttgc catttagttg ttactccagt ctaaatgatt ttattgacat
     1261 cattttagga catattatgt ctaaaaacaa tacctaaaac agataatttc atgcctataa
     1321 acagataatt tcatgcctat aaacagataa tttcatgcct ataaacagat aatttcatgc
     1381 ctataaaatt ttgtatccct tgggggagta aggacacagc cgacggtaaa gaggtttaaa
     1441 aagaggttta aaaaaagatt ataaaagagg gagacaaaat ttcccaccta aaaaaacaat
     1501 tattttttct ttaaacaggt tcgcgaactt ttctaaactg ctaattcaaa acctcgctcc
     1561 ctacggtcgc caaaaaggtg tgatcgctac gctctcacat tatacctgcg cagtcgcctc
     1621 cagctccttt gctaaaacca ttgttcaaaa aggagggttg aggctctaag agcttgtcta
     1681 agccgtttta agacagtctt agtataaaaa cctttatctg gcatttaaag cgccctacag
     1741 gcggtcaggg caagccctaa caacccggaa ataagagata cttaaaattg ccacacagat
     1801 ttaattttgt agggtataat aacttgtcgc ctatccgaca gtcttgcgac tggaaggagg
     1861 tgtatctgat gtttgaactg tttatttgcc cccttcttgt aggagtttgt atctctgtat
     1921 tcagttattg gttgaataac cgaaacaaga agtaaaatca gtaaaggcga catcaaccta
     1981 aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtatctaatg tttgaacatt
     2041 tgcatgttta gtttaacatg aatttcaatc aaattcaatt ttgctgttgc tgatctaacc
     2101 attccttgac gctttttaaa taatttaaag ctgtttcttt tttagtcaga ttttgtatgt
     2161 taattgcttg ttgaagcaag atttcatagc gttcatatgt gactgattgt tgtcgttctt
     2221 ttcgttcttg ttcgctagtt gaaaaaggaa ttgtatctcc aaataaatca ctacaagctt
     2281 cttccacttt cgtatctttc acatattgca cgtctttttt tacttgttca tatttttctt
     2341 ggtactcttt ttgctgttct tctggcaaat tccgataaag tcgagtgagt gtttccatat
     2401 ttttatccgt caaacgcttt tcttgcttca caaaaatatc attcatgtcc tcataacaag
     2461 cggtaaattt ttctattttt tgtgctaata cttgctcttt tttcttttta tcacgcgctg
     2521 attcatactt ttgagtgaca tttcctaata aaatacttct agaaacgcgc ttatttccct
     2581 ctaaaacgcc gaataagccg cttgaaacga ttatcaagga aaatatacag ccaagaaaga
     2641 aaaatcgctt aaatcgaatt ttagagcctt ttaatgaata tcttgtttga aaaattttat
     2701 agatgagcca aatacttcct gaaatactaa caaaaatgag tagatagggt aaataaaatg
     2761 ccattttagc ctccccatac taaccaaaat gcgaacagtg tcaggactaa gcaacataag
     2821 ggggcaagaa aatccaatga tcgttgaata agcacctttt tctgttcctt atttcgctgg
     2881 tctaatagct cttgatggag ttttttaact tgtaaatcaa tcgtagtttg tatcctttct
     2941 tgcgtataag gaacgagaaa agcacgttgt ttctttattt cgttttctat gtcttttaac
     3001 gttttagagc gataattctt gttggtggtc tgttcctctt tcaacgtgtt gttcatggtt
     3061 tcttcgagcc ggtctaattt ccggttgttc aggcgtaaga gttcgttgtt ctcttcgtac
     3121 ttcttctgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg ttcgctgttg
     3181 gttataggtt cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt cgtagtcctt
     3241 tcctagtctg ttggatcgta cttttttctt tttgtggatc aattcgtaag tgaaattttt
     3301 cccgcgattg aagcaatgaa tccccaatgt tttgagggaa ttacaaaatt ggtcataggt
     3361 tctggttttt ttgtttttca ttgtttgatc gatcgcaatc cgaatttcgt ctttccaaac
     3421 aggttcattt ttttctttta attttatttc agacatggtt cgtttttctg gtttctttcc
     3481 tcgctcagtg attggtaagt tatgtgcaag acaaatctcg tcatttaggt cataacattt
     3541 gtttagaaat gcttgaccat gagcttgaaa tttttttcca gtctccagat taacggagtt
     3601 aatgacaatg tgattatgaa tatggtcttt gtctgtatgt gtgtaaatag ctatttggtg
     3661 atcgggggct attttttttg catactctgc ccctagttga ttagcttttt cagcggtaat
     3721 ttctcctggt cgaaaagatt gaatcaataa gtgtgcctgt acacggtcgt cttttccata
     3781 cagcatgcga atttgtttca tttcactttt ggcataatcg acgtcacagt ttattcctgt
     3841 tttaatggtt gctcgtggtt cagcgtaatt aattgcacga ctacaagaag tggttcggct
     3901 tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag gttttcggat
     3961 aagcgttggg cgtctccagg agcaacgtcg tgttggtgtg tattacacca cttagcaatt
     4021 tgattgatat tccctccagc tttggctaaa tcgcgcgcga ttgcctgggc aatgtctggc
     4081 gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg cttttacgaa agccggcaca
     4141 ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcagattc gctcactcga
     4201 aaactgattt gttttggctc ggatcgatag gtatattttt tagaatgatc gctagccaaa
     4261 ttttgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga cgtatcgttc
     4321 gcaataccat tgtacctaaa aaggtacaat gagtaaagct cactaaaagg gtttggcaag
     4381 ctctatattt gctagccaaa tacctacgta tttggtgcaa ataaccgctt ttcaaagggg
     4441 cgcaagctcc cccttgaatc cccctcaaaa cccacgctcc acaatacaaa aaaggctagc
     4501 aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa ttattgtttt
     4561 gtaatatcaa acaactattc atcaatcagc tttaaaaaat tcaattggtt tagctgtccc
     4621 ttctgtccca tcattcgttg tcaaaataaa tacgagcttt tttggagaat ccaaactaaa
     4681 agtgtaatct aattgttttt tattagagcg cagtgttagt aaattatttg ttgctttgta
     4741 aatgaaaact tgttcattta tatttaaatt tttatcaatg atttgaattt tatgattgtt
     4801 ctcttttact aatgttctat atttttgaag tatttttgaa gtatttaaat tagtgattac
     4861 tagacgttct gaatgatctt tagtttgcca agttcctaga aaaggaatca gatcagtctt
     4921 ttgtatttct tgtttttttg aacaactagt taagaaaaga actaacacta gacccataac
     4981 taaagcaatg ggtttattta ccaatttgta atggtattgt cccaagtaaa accacctcca
     5041 ggtacaggaa ttgtaactgg tcctgcattt gcacttatta cagttgggta gttataccaa
     5101 ggattccaca catagtaagt ttgataacca ccttgattga cccacccaaa taatgcaagt
     5161 gcatgacggc tgttactata atttccataa ccagcagttc ccatataaat ggcatttcct
     5221 tttcttagtt gtgtttggac ctctgaaagg tttagtattc gtgctacttt tttaggataa
     5281 acaccacagt aatttgcata ttttattatt tgatcttgat taagactttt tgattcaatt
     5341 tgactgttgt ttaaattagg ataggtataa tgcataatgg ctctagctga agtagcatgt
     5401 acatctgttt tgttacataa gatagcagct tctacgtaag cagcacacca cggaaaactt
     5461 gtttgggttt ctgctatacg ccaaatctcc ggaggagagt tattttacac gtttaatcgc
     5521 aggaaaattt tgttttttag agaaattttt aattttattt tttatattta cggatataag
     5581 atcatttttt tctgtaaagg aagaaacacc atttttttct gtacttaaag gtgatttcca
     5641 aagtagttta atttgtccat tatcggaata ataaatgcta ttatcttgaa aaaataaggt
     5701 tgtatctgtt atattactat ctactagatt ttgaagttct tttgctaaaa attgtgaaat
     5761 acttcctgaa tattctccat ttgtattttt ttttgacggt ataaatatat ttaatcgtaa
     5821 tttcagcgta ttatcagcgt ctgaaaatac tggaaagaaa atagaactat ttgccgtatt
     5881 ttctccatcg agcattttaa atggcgaacc aagagtatag ttaatatcag tttcggtgtt
     5941 attttccatt aaaagaattg aagttatgga gtgttcaaaa tcagttttcg caaaattaat
     6001 tgcttgttct tcaattggtt gggagttgat attgaaatta gaattttctg aagcataggt
     6061 atttgtaatt cctatactaa aaattccaat catcaaggaa aagaggagac ctagataaat
     6121 tttctttttg ggtttcaaaa taatcacgcc tttgtgttaa atttaagtat aattaaaata
     6181 tacaatatgc gataacaaat ttttgttata gttcataaaa tattgttttg ggaacataat
     6241 cgttatttat agcgttagta aacatatagt taacataatg tgtagtatgc caagtagccc
     6301 ttgtatcaac cgatacaggg gctcactttg tgatttttaa ttggtctagg taaaaagctc
     6361 tttttaaggc ttgattttct gcagttgctt ctttccgttt ctttgtgaat ttttaccagt
     6421 ggattgttat ttttcataaa atatgataaa ttttttgtag cgctttataa gcgcgttgct
     6481 ttttcaagaa taaacactca ttatttttta actggctgct cgaaagagca gtctttttat
     6541 tatttatccg tttgttttac aaacaattct tcgttaggac tgttgtaata atttgaattt
     6601 aatttaagtt ctctgttgga atgttggaat tgttttttcc aattacggat aatagcatgg
     6661 ggctaaaacc taatattcct agagaaatag aacccagtac caacacagtt ttttttaaca
     6721 taggttatac ctccactttt tttaacgcta ccacttgtat acacatttta aatactagcc
     6781 ttcagtttat caaatgttta aattgaacgg atataaatca aaatcaaatt ttcaaaagtt
     6841 gttctaaaaa tttttctttt ctggaagtta ctctaacttt ttttgcttgt ggcgcttggt
     6901 atgttttctt tgccgatcgt ttaggtttct gaccattttc tctcaacgcg gtaaaaaccc
     6961 cacttatgag agaaaatggt cagaaacctg ggaggcggat aatgccttta ataccaagcg
     7021 ccgcaagccc ctccgcccat ttgtcatggg ggggtacatg ggtaatacgt ggataggtac
     7081 atgggtagta cgtgggtaaa atttctcaaa aaaaataaac gaaatttcaa cctattttaa
     7141 gcaacaagtc accatatagt ttgataattt acaaccgtaa acaacaattg tatgtttctt
     7201 cagaaaaagt agttgattca gaaaaagtag ttgattcgtt tttattaata attttgagtg
     7261 attattttcg cccgtaattt cattcaaaaa aattgtttat ttttttgatg aaataaatag
     7321 acattcctat aattactggt ataatgacaa aacttgttac taaatttaaa gtttttaaag
     7381 ctaatgttat taacaatata tctaataaaa ttacggataa tactaatata gcaaatgatc
     7441 tatttttcat aaagataacc actccttttc aaattaaaaa catcctctct atacgctatt
     7501 ttattgtata gaaagaatgt tttgttttta ctaaatgtag tatttggctg ttctactact
     7561 agcattccaa ctcataaggt aacaagtacg catagtttgt ttacctctaa tatttactaa
     7621 cattttaaca gaattaggca gaagtcgccc atcctctata ggtgggtgat gaatgccgtt
     7681 cggtatgagg gttaccgttg gtaactcgaa gaccgacaac taaggcgtat tgccttttta
     7741 ttttgttcca tgttacaatc agtctataca agaaatgagt tgaaattgat ataacatgga
     7801 attagataca aattcagtat ttcttcttta ttaccacctt gttttagtaa cgaaatatcg
     7861 tagacaagtg attgatgatg aaatatctga ttatgccaaa actacttttg aaagaatttc
     7921 agaatcgtat cacattactt tagtggagtg gaatcatgat aaagaccacg ttcatattat
     7981 gttcaaagct caacctaaaa cagaattgac aaaattcatc aatgcctata aaagtgccag
     8041 ctcaagattg ataaaacgtg actttccaag agtcaaacaa tttctttgga aagaaatgtt
     8101 ctggtctaaa agtttttgtc ttctgacaac tggtggcgca ccaattgatg tgattaagaa
     8161 gtatatccaa aatcaaggaa acaatcataa atagaaagta ggtgaacttt atggaacaac
     8221 taaaagcata caaattcaga attcagaatc tatccaacag aggaacaaga aatcttcttt
     8281 gccaaatctt ttggttgtgt ccgtaaggtc tacaatctaa tgcttgatga ccgaaaaaag
     8341 gcgtatgaag aagttaaaaa tgattcttct aaaaaaatga cttttccgac accagctaaa
     8401 tacaagaaag aatttccgtt tttgaaagaa attgatagcc ttgctttagc caatgcccaa
     8461 cttaatttag ataaagcata caagaatttt tttcgggata aatccgttgg atttccacgt
     8521 ttcaaaagta agaaaaatcc tgttcagagt tatacaacga ataatcaaaa tggtacagtt
     8581 gctttgattg acatcaaagt tcctaaattg aagtcattag taagaattaa gcttcataga
     8641 caaccaaaag ggattatcaa atctgctaca atatcacgtc attctagcgg taaatactat
     8701 atttctttgt tgtgtaaaga agaaattatc gaattaccta aaactaattc cacaattggt
     8761 attgacttag gtattatgga ctttgcgatt ctttctgatg gacaaaaaat tgataataat
     8821 aaattcacgt ccaaaatgga aaagaaacta aagcgtgaac aacgcaaatt gtctaggcgt
     8881 gctttattgg ctaaaaataa aggtaccaat ctatttgaag ctaaaaatta tcaaaaacaa
     8941 aaacgcaaag tagctagatt gcatgaaaaa gtaatgaatc aacgtactga ttttctgaat
     9001 aagttgagta cagaaatgat caaaaatcac gatattatct gtattgaaga cttaaacaca
     9061 aaaggtatgt tgcgaaatca taaattagct aaaagtattt ctgatgtttc ttggtcaaag
     9121 tttgtgacta aattacaata caaggctgac tggtatggac gaaaaatcat caaagtagat
     9181 aaatggtttc cgtctagtca gatttgttca gagtgcggac ataaagatgg caagaaatct
     9241 cttgaaattc gagaatggac ttgtcctgtt tgtcatactc atcatgatcg tgatatcaat
     9301 gctagtatca atattctgac cgaaggtcta agactacatt ctatgggatt ggcttagatc
     9361 caatcatcaa gaaccgtagg aactacgggg atagcttggt aaataagaga caccgctgtt
     9421 agtaaagaaa tacgctatca agtatgctct attcccaaga agctcccact tcaagcgtta
     9481 agaaccgtca ggtttagcta agtggtgagt agttcacctt tatttggcac tttagcacct
     9541 tttggatatt ttttgtagat atatcctctt tgagcagcag cttcaaccac tccaccattt
     9601 tgagaaaatg aaacagaagg ggtgaaagct aataaactgc taaacaaaca caaactagct
     9661 accaataatt ttgttttttt caaattatga cactcctttt ttaatgatta actttaaggt
     9721 atcataattt ttattgaaat agacattcta ttcataaagt gttattttga attcattttt
     9781 gtgttccttt tttttcgtta gtatatctac tacaacacaa cataaatccc aaccccaaaa
     9841 aattactaag aatggcagaa ccaccttgac taatataggg gagtggaata ccagttagag
     9901 gtagtaatcc gatacttgca cctatatttt caaaaacatg aaaaaaaatc attgcaacaa
     9961 tgcctatacc gatataggtg aaaaataagt tattgctttt aaaacatgtt ttatacattt
    10021 gataaattaa ataaaagtat aggaatatta caagacaact tcctataaag ccaaaatttt
    10081 ctccaattac agaaaatatc atatcggatt ctctaacagg aacgtgaatg tttgatacat
    10141 taaaaccagt acctaaaagt ccgccagagc caatagcaat taaactattt gctaactgat
    10201 agctagatcc atttggatct aaaaatggat tcaaccatga gtctattctt ttaaactgat
    10261 agtcgtgaaa tcctacctat ttttaataag aaatctcttc ctgtatctga tataaccaaa
    10321 tataaaaata ttaaggataa aacaacacca atggcgatta ttggggcaat tatttttata
    10381 tttatgttag acgtaaacaa aataaaaata taaataacaa tgaagactaa cattgttcct
    10441 agatcgttct gaaaaaataa taatattcct attggaagag tagcaataaa agttttaagt
    10501 aaataaatcc aatcatcttt cgtattacta gtaatagatt tttgaattga cggttatttt
    10561 agctagcata agggtgtatc ctattttgac taactcagta ggttgaaacg aaaaaccagc
    10621 aatataaaac caattttttg ctccagttaa gactgctagc taattgtgga ttatagaatt
    10681 tcaagagaag taataacatt atgatgcata atccgtaaaa tataggagtt aatttccaca
    10741 aaattttagg attaaaaaaa taaatagtta ttgctgaagt gaacccaata ctataccaca
    10801 atacttgttt aattaattct tttgtgattt ttgaattagt gtaagagtcg tgatataaag
    10861 caacatataa agaaagtaat ccaatgatac atagcagaaa aactggcaat aaaagcccat
    10921 aattcaactg atttttgtag attctattat ttttcatttc gaaaggtcct ttttcataat
    10981 ttataaataa aaataacaca ataaagttat ttttactagt tttgagtttt agacgaaaat
    11041 tacaattagc tactttttta tcttactaaa tagttctatc tttttatata aagtatagaa
    11101 aaattaataa aacaattgga aatatgctgt gaagaaataa gtttctatgt ttattaaaga
    11161 taataattaa agtattaatt attaatagta aacttagaaa aaatattaat aaccaagtgg
    11221 caattgaatg gctaaaataa aagaatccgg actgaattaa ggtgaaaact aaacttaaaa
    11281 aaattacaaa atgaaataat agtattagtg tttttgtatg attttttacc ataatttttt
    11341 ttcttctcct aacaaaatat tgtctagtga taaatcaaat aacttagcaa actgaattaa
    11401 attttctgta tctggtattg ttcgaccatt ttcccaatta gaaatgtttt ttttagatac
    11461 taaaagcatt tcagctaaat cttcttgagt tagttgtttt tctattcttt tttgtttgat
    11521 tactttactt atagtcattt gatcacctaa acatattttt tattcttaga aagatagagt
    11581 ctatagaaat tgatttcttc tttatgttac caaaatttcc accacttttt ttctgttgtt
    11641 attgcattgt tgttttcttt attgacagaa gttaattcct cttcttcagg ttcttccgta
    11701 taagctaatt gtaactgttc ttgcagtttt tcgatctgtt gatttgcctg caaggtgagt
    11761 tgttgttgct ggtcgagtaa cttttgcatt tgttttattt gacatttgtt ttattt
//
