LOCUS       AP026626                2921 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA96 plasmid pAA96_8 DNA, complete sequence.
ACCESSION   AP026626
VERSION     AP026626.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466810
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA96"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA96_8"
                     /geo_loc_name="Japan"
                     /collection_date="2011"
     gene            complement(60..476)
                     /locus_tag="EfmAA96_35530"
     CDS             complement(60..476)
                     /locus_tag="EfmAA96_35530"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117491.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDP75768.1"
                     /translation="MRVYEEGMASHNEKEFSVKKPKVRRVANKQISFRVNEAEYEKLQQ
                     SAKNLNMSVPAFVKKKAQGAKLVRPVVEQEIALEISRNLKVLGNNANQIARRLNSGTSS
                     DNEELNAEVQRVLKGLGEIWRLLNSEIQNKRQER"
                     /name="mobilization protein"
     gene            complement(785..1192)
                     /locus_tag="EfmAA96_35540"
     CDS             complement(785..1192)
                     /locus_tag="EfmAA96_35540"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 1334"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75769.1"
                     /translation="MGNNSVAHLEIIDHIKGSYEYLTHESKDAKEKNKHVYDKKDILHI
                     NDFDIDRYISLDENQKRELGNLVLSLIRKYELENIIDLGDFVEMRGEEYGLPSMEIIND
                     ALTSRTGLLRLYFDGNYQRRKSGKPNWLKEK"
                     /name="hypothetical protein"
     gene            complement(1305..1466)
                     /locus_tag="EfmAA96_35550"
     CDS             complement(1305..1466)
                     /locus_tag="EfmAA96_35550"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 1334"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75770.1"
                     /translation="MNKNITKARHFGFLLYPESIPENWREQLESLGVSMAVSPLHDMDE
                     NTNKKELD"
                     /name="hypothetical protein"
     gene            complement(1547..1696)
                     /locus_tag="EfmAA96_35560"
     CDS             complement(1547..1696)
                     /locus_tag="EfmAA96_35560"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75771.1"
                     /translation="MAESERLVVILPSEIKRKLEEKAKDKSLSMSTYVKLLVTEDLKKS
                     EKGN"
                     /name="hypothetical protein"
     gene            complement(2230..2919)
                     /locus_tag="EfmAA96_35570"
     CDS             complement(2230..2919)
                     /locus_tag="EfmAA96_35570"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75772.1"
                     /translation="MKFTRSSFGKDSGIQAHTIIQSFSPEESKGLGAVTINELGVELAR
                     KVAPEFEVAVYTHTDKEHIHNHIVINAVSFETGKKYQSNKAQRELVKTKNDEIARAFGL
                     SVPEKKAEIRYTQAESEIAQDGRDSWKVDIREKVELYRTTMELERSESSFKAFMMERGI
                     NVSFRGKNVTYEHLEANRKVRGAKLGGRFDKEALDKMFGSKEKELEKDKQRRYKEYLRQ
                     QGRHLEY"
                     /name="hypothetical protein"
ORIGIN
        1 cacattccaa ccacccacat tccaaccacc tttcactgtt gcacgtttct ccgcataatt
       61 tatcgctctt gacgcttgtt ttgtatttcc gagtttagta gtcgccatat ttcacccagc
      121 cctttcaaca cacgttgcac ttccgcattt agttcttcat tgtcactcga agttccgcta
      181 ttcaaacgac gtgcaatttg attcgcatta ttccccaaca cctttaaatt tctgctgatc
      241 tccagtgcga tttcttgctc gactactggt cgaacaagtt tcgcaccctg tgcctttttc
      301 ttcacgaatg caggcacgct catattcaaa tttttcgctg attgctgcaa tttttcatat
      361 tcggcttcgt tcactctgaa gctaatttgc ttattcgcca ctcgtcgaac tttcggtttt
      421 tttaccgaaa attctttctc gttatggcta gccattcctt cttcgtatac tcgcataaag
      481 tcccactcct tcttttgcca cgtggctagc cacgcaccaa ggttcgattc aaaattgttt
      541 gtttttcact cacaatttta aatcgaacga aggagtttgg caagcgtcgt atttgctagc
      601 cacttccttc ggaagtggcg caaacaccgc atgcaaggtg ggagctttca cccccccttg
      661 accccctcaa aatccacgcg ccacaataca aaaaaaggct agctcatgct agccaatttt
      721 tgtaaagttt tgcgatttcc caaaatcaaa aaagccggca ccctgcgccg accattttca
      781 tcttttattt ttctttcagc caattaggtt ttccagattt tctacgctga taattaccat
      841 caaaatacaa tcgcaaaaga ccagtcctgc tagttaatgc atcatttatg atttccatgc
      901 taggaagtcc atattcctcc cctcgcattt ctacaaaatc acctaaatca atgatattct
      961 ccaattcata tttacgaata agagatagca ctaaattccc taactcgcgt ttttgatttt
     1021 catccaaact aatataacga tcaatatcaa aatcattaat atgcaagata tcttttttat
     1081 cgtatacatg tttattcttt tcttttgcat cttttgattc atgcgtaaga tactcatatg
     1141 agcctttgat atggtcaatt atttctaagt gagcaaccga attatttccc aattttctct
     1201 tgattttctt tcttacgctc tcgattgtta caggattgcg agcaatataa aggacatgat
     1261 aatgtggctt tacataatgc ttaccgttcc tgataacatc actactagtc caattccttt
     1321 ttatttgtgt tttcgtccat atcgtgtaac ggactaaccg ccattgatac tcctaagctc
     1381 tcaagctgtt ctctccaatt ctctggaata gattcgggat atagtaaaaa accaaaatgt
     1441 ctagctttcg ttatattctt attcatgata tacttacctc aattgaacac tatcagttaa
     1501 gcggaaacgg tgcgccaaca catatgtttc tgctcttttt ttgcgctcaa tttcctttct
     1561 ctgatttttt caagtcttct gttactagca atttaacata tgtcgacata ctcaaacttt
     1621 tgtcttttgc tttttcctca agttttcgct taatttcact cggtaaaatt actaccagtc
     1681 tttctgattc cgccatgtct ttcagcccct ttagttaatt gttaatatca tggtaaactt
     1741 ttgacaactg tattgtcaat atttttttct atttttcaaa acggcggtca aaacggcggt
     1801 caaaacggcg gtaaaacggc ggtcatctta aaatagctgt atcccttggc agagtaaggg
     1861 cgaaagggtc tgacatcaaa atttggcact cggcacctaa tggggggggg tcgtagtacg
     1921 gaagcgaaat tcgcttcctc gcccccccga ttcgtccaaa ttacagaaat ttttagcaaa
     1981 atttcttcca gcgctaccgc tcggaaaaaa acaaaacctt gccctttgaa aaggtcaaat
     2041 ccaaactttc gctaacgcta ggttgcccaa aaaatggacg tgtccatttt ttagcaaaaa
     2101 tgagcttctg ggtttccgtt acgtcgtcaa ctccttaaag cctcaaagag gggctaaaaa
     2161 cgcacagcat gcgttctaag cccctctaat tcgatttacc gttgacaaca cttatctcgc
     2221 ttccttcgcc taatactcca aatgacgccc ttgttgtcgc aaatactcct tatagcgcct
     2281 ctgtttgtct ttttccaact ctttttcttt agatccaaac atcttatcga gcgcttcttt
     2341 atcaaatctt ccaccaagct tcgcccctcg aaccttccta ttcgcctcta aatgctcgta
     2401 tgtgacgttt tttcctctaa acgatacatt tatacccctt tccatcataa acgccttaaa
     2461 cgagctctca gagcgttcta gctccattgt tgttctatac agctcaactt tctcacgtat
     2521 atccactttc cacgagtctc gtccatcttg cgctatttca gattctgctt gggtatatct
     2581 tatttctgcc tttttctcag gcacagagag accgaacgct ctagcaattt catcattttt
     2641 tgttttcact aactcacgtt gtgctttgtt tgactgatat tttttccccg tttcaaaact
     2701 cactgcattt atcacgatat ggttatgaat atgctccttg tctgtatgcg tataaaccgc
     2761 tacctcaaat tctggcgcta cttttctcgc caactcaacc cccaactcat tgattgttac
     2821 agcgcccaaa cctttacttt cttctggaga aaacgattga atgattgtat gtgcttggat
     2881 tcccgaatcc tttccaaaac tcgaacgagt gaacttcatt t
//
