LOCUS       AP026624                4372 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA96 plasmid pAA96_6 DNA, complete sequence.
ACCESSION   AP026624
VERSION     AP026624.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466810
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA96"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA96_6"
                     /geo_loc_name="Japan"
                     /collection_date="2011"
     gene            complement(208..753)
                     /locus_tag="EfmAA96_35440"
     CDS             complement(208..753)
                     /locus_tag="EfmAA96_35440"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75759.1"
                     /translation="MTGDLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELTEMIRDQQ
                     KLTLLQQQKNQSVVTRKSRIKKSKMVAILEVRCDMDKIDLREETVDRIELMYERLADYL
                     NLPDEDNYSEMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMS
                     HVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            complement(750..944)
                     /locus_tag="EfmAA96_35450"
     CDS             complement(750..944)
                     /locus_tag="EfmAA96_35450"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75760.1"
                     /translation="MTDLSEKNNHKKLADELGVSKNTVRNQLVNSGYDLARYKENGVIM
                     LDKELIGVVRSIYRSKVVN"
                     /name="hypothetical protein"
     gene            complement(937..1170)
                     /locus_tag="EfmAA96_35460"
     CDS             complement(937..1170)
                     /locus_tag="EfmAA96_35460"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75761.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDNVVVVFLDSKKMSGKLLDVTQYELI
                     LKNNKIDSPIIIPKHAVKYIYAVAESNGEAND"
                     /name="hypothetical protein"
     gene            complement(1214..1687)
                     /locus_tag="EfmAA96_35470"
     CDS             complement(1214..1687)
                     /locus_tag="EfmAA96_35470"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 1652"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75762.1"
                     /translation="MSLLHNIGWLILSFKPIYGRSGKLETAYWGSRASERQIRMYNKKL
                     EQERKRKIVPPEITTWWRLELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIM
                     VKGLLYDHSEWGNLAKNTKKKYRDLLKQESQNDELTNHLRETFSESADDLKKN"
                     /name="hypothetical protein"
     gene            complement(1644..2138)
                     /locus_tag="EfmAA96_35480"
     CDS             complement(1644..2138)
                     /locus_tag="EfmAA96_35480"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75763.1"
                     /translation="MSKKGIIVNEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVGK
                     LKENIYYHTQNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKWQE
                     GKGRIDFNPNKIGHFLASSMKNFIHDLFLEPHFSRADIACDIIDVPDEFITQYRVVDPV
                     F"
                     /name="hypothetical protein"
     gene            complement(2586..3992)
                     /locus_tag="EfmAA96_35490"
     CDS             complement(2586..3992)
                     /locus_tag="EfmAA96_35490"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75764.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKTNDWHKHIDFTKSDENI
                     YLEQTNIGEKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFRTNRADGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIERLMA
                     DFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENEREK
                     ALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="hypothetical protein"
     Typing          complement(1184..2114)
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.453"
                     /coverage="98.0"
                     /evalue="0.0"
     Typing          complement(2590..3992)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.581"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttttcgtgt tgctgatcgt cagaaatcga cgatagccac cgctcaaaat cagcaaaagt
       61 ttcaaaccca aaacgcttcc gaagagcctt gcccattgca gcattttctt tctcaatcaa
      121 gcgttttttc tgttggcgaa gtttcgcttg ttcttttttt aatttttctt cttgcgcttt
      181 aatatctgct aaagtcattc ccataaacta catctccttt tctttggaat taataacgga
      241 aatttggttt aactgcataa catgactcat taatttcaac caaaattctt tttctgattc
      301 atcagttgtt tgcgctaaat gcttatgaac aaattcatta gtcaaaactg aagattttgc
      361 taaatcagca ttgataaccg agttgagaac atccataatt cgttcaagca tttcactata
      421 attatcttca tccggcaaat tcaaataatc agctaaccgt tcatacatca attcaattct
      481 atcgactgtt tcttctctca aatcaatttt atccatgtca cacctcactt ccagaattgc
      541 caccattttc gattttttaa ttcttgattt tctagtaaca actgattggt ttttttgttg
      601 ctgaagcagg gtcaattttt gttgatcacg aatcatttct gttaattcag aaatatgttt
      661 gtctttctca gctaattgat cacgcaaaaa ctcattttct ttttctaaag gaaaatcact
      721 gcctgtatta ttgaccggta aatcaccagt caattgacca cttttgaccg ataaattgac
      781 cgaacaacac caatcaattc cttgtctaac atgataaccc cattttcttt ataccgagca
      841 aggtcatacc ctgaattgac cagttgattg cgcactgtat ttttagatac acccaattcg
      901 tcagcaagtt ttttatggtt atttttttct gacaaatcag tcattagctt ctccattact
      961 ttcagctacc gcataaatat atttaacggc gtgcttaggg ataatgatcg gagaatcaat
     1021 tttattattt ttcaaaatca attcatattg cgttacatca agtaacttac cggacatttt
     1081 tttgctatcc aaaaaaacaa ccacaacgtt atcacctgtt aaattcacca accaattttt
     1141 cttaccaaaa tacttttgtt tttctgtcat ttttcttcct cctctgttac gtctagccct
     1201 agaagccatg tgtctaattc ttttttaaat cgtctgccga ttccgaaaag gtttctcgca
     1261 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     1321 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     1381 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     1441 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     1501 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     1561 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     1621 taccagaacg cccataaatt ggtttaaaag acaggatcaa ccacccgata ttgtgtaata
     1681 aactcatcag gcacatcgat aatatcgcaa gcaatatccg cacgtgagaa atgtggttct
     1741 agaaataaat catgaatgaa atttttcatt gaactagcta aaaaatgacc aatcttattc
     1801 gggttaaagt caatccgccc tttgccctct tgccatttta aaatttcaat ataagcaata
     1861 ttctcttcat gttggtctaa caactgccaa ccattattac taacagatct taaataacca
     1921 ttaccttcat ttaggcgcat aagctgttca aaatttaaaa tcaacacatc attttgagtg
     1981 tgataataaa tattttcttt cagttttccg acgatagtaa ttcggtcaat tgaccactgc
     2041 atttttaagg gaagggtcaa actttgatac tttaaccgcc tgttagaggg ggctgttaat
     2101 ttttcccact catttacgat tatgcctttt ttactcaaat tttgttcctc ctcaaagaaa
     2161 aagagacttc tggaaagaat tttcaaaagt tcacgtaact cttaaaaatt cattaccaaa
     2221 aaatctctag tcttgaagaa atctaaaaat attcgtataa tgattaagaa gttacggagc
     2281 tagtgcgatt agtttccgta aaggtcgaga acgtgggcag cgttgctcga cttatttttt
     2341 tactcttttt tcagtttatt tgtttgctaa ctcaataata gcacttctct ttagctatgc
     2401 aaatatccaa aaaataaaac cgcacccttt gaaaagggcg cccccaaact tcgctaacgc
     2461 taggttggcc aaaaaatgga cttgccattt tttagcaaat gtcagcttca gagattagtg
     2521 gtttagccgt caactcctta tcctcggcac aaaccgcctc cggtaccgtt gactgcacat
     2581 cgtaactacc actcgatctc atcttcagaa cgctctaatt tacgctgtga gacgttttga
     2641 cgataaaata aggttttctc ctgctctaac acttctaatt cctcagaaag ccccatatcc
     2701 ttttctgcaa gaccaaaacg gatagacaaa cgagtgatcg atttttgaaa tgcgtttttt
     2761 aatcgctcta aacgctggcg taaagagcga ttttcttctt tcagcttttt attttccact
     2821 tgtaaatctg ctaattgttt tctaccagac aaatgacgct cttcttccaa tgtagctaca
     2881 tactcattta attctgtatt ttgctcaagc atctgtaaat attgctcact catgttatca
     2941 gcatttctct tggcatttgt tgctctcgta gctcgttgat acaaatcaac aaaatcattc
     3001 ccagataaat gatacgaacc atcaggaagc ctcttgaaac tttctaaagg aacaatatct
     3061 ttttggaaaa tgaccgccat ctcttttttt acattcagca tatttttctt ctcttcttca
     3121 atcttcactc tctcagaagc caccagagcc ttttctcgct cattttcttc tctttgccta
     3181 gaaatatacc tcaactcatc agaaacgctc tcacgctctt ttacggccgt ttgacgcaat
     3241 tctgaaatct cactcatgat ttctttatat tcgtgaatat ctttgatttt gttagtaccg
     3301 actaactcac gctcccaacc aaaatctgcc atcaaacgtt caattgactc aacttcctga
     3361 ttcctaaaat tattccaaat cactcgacca ttctctttat ctgctcgtaa accctgctgt
     3421 tgcaaggctt tgttaaaact tggttgcttt tgaacacccc gtttataccc ttcagcaacc
     3481 ggaacaatat tcaaatgcag gtgcggactt atttcgtcat tatgaataac agcattgtag
     3541 acggataaat taggattccg ttctttaaat tctttgaaat attcaactaa aattttgttg
     3601 gcaatcactc gattttgttc agcttgttgt tccgatatgc cagtagaaga accatcagca
     3661 cgattagtac gaaaatcatc taaaatgcct atttgcacaa taaattctcg ttgaggttcc
     3721 aactttttat ccttgcgcac tttcgctaaa tagtcctcga tttttctatc ggctcttttt
     3781 tgtttcgcat tatatttttc tacggcttca ccgaataatt cttcatattt ttctcctata
     3841 tttgtttgct ctaaataaat attttcatcc gattttgtga agtcaatatg cttatgccaa
     3901 tcattggttt tctggctatc agttaactca cgattattat gagaaatgct tgtattcttt
     3961 gttccttttt tgaatgaaat actgaacccc aagttaaaac cctccttcgg ctgtcgctaa
     4021 taaaaagagt gtaaaccttt ctgttactct ttgtcaaaga gtaacatttt cctaattgtg
     4081 acatggctgt cacaattcca aatgctctcc gagggtattc gctgtcacaa gtgacaacga
     4141 aaaaaggagc cgaaagcgtg tcacgctctc aactcctttt acgctttatt ttgcttcacg
     4201 caaaataatc gcttatcgta aattcacgtc attaaatatt gacgtaaatt tactctcact
     4261 catctgactt ttcttattcg gtatcactcc gaataagccg tcatattcat tgggataatc
     4321 tcgttcgctt ggcgctgatt tttcatcagc aagccatttc atcagcaagc ca
//
