LOCUS       AP026623                5072 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA96 plasmid pAA96_5 DNA, complete sequence.
ACCESSION   AP026623
VERSION     AP026623.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466810
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA96"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA96_5"
                     /geo_loc_name="Japan"
                     /collection_date="2011"
     gene            complement(289..570)
                     /locus_tag="EfmAA96_35400"
     CDS             complement(289..570)
                     /locus_tag="EfmAA96_35400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390111.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75755.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESDF
                     DRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
                     /name="hypothetical protein"
     gene            complement(1017..1985)
                     /gene="rep"
                     /locus_tag="EfmAA96_35410"
     CDS             complement(1017..1985)
                     /gene="rep"
                     /locus_tag="EfmAA96_35410"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP75756.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQF
                     TFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKIDGK
                     SDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFAYFS
                     KEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELLQQLEK
                     LEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
                     /name="rep"
     gene            complement(3152..3307)
                     /locus_tag="EfmAA96_35420"
     CDS             complement(3152..3307)
                     /locus_tag="EfmAA96_35420"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75757.1"
                     /translation="MKLTKLTISLVIIAGIILYFVLPANQFFLYFGIPTILIVNGVRIF
                     EQRKKM"
                     /name="hypothetical protein"
     gene            complement(3888..4343)
                     /locus_tag="EfmAA96_35430"
     CDS             complement(3888..4343)
                     /locus_tag="EfmAA96_35430"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75758.1"
                     /translation="MKKQLFKTFLLSTSILTFGSAITPSGAVFANENNISDIATYSDDE
                     YYYVLDEPVIQTRTWSKPQSWSIAKGSRTYLGIETHSRGTKLSVSANVFGMKIEIARNT
                     SASGKFKKYRQNARITVTYRVYRKVDNKYIRTQTSTANTQYIDYIPA"
                     /name="hypothetical protein"
     Typing          complement(1022..1985)
                     /name="rep_cluster_1093"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.679"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tgatacactt tagaatttgt accgataaga acaccagtat cttttagata taaatcagca
       61 aattctgcca tgatttctgt catgatagaa atttcttttc ccatggcatt gagttttgct
      121 tggttactgg ttgtgattgt attggtatca ggttcataac tcatacataa aaaacgaatg
      181 gcttctgaat aattgttgac gtctggattt ttttcgatga tggaatctaa aacgtcaacg
      241 tgactgggat aaagggttac gtgtttttga acagaagttt ttctcatgtt atattcctcc
      301 gataagtgtt tcaatgagtt ggttgtttgt gtgaacatag tcattcagta tgttcgtatt
      361 ttctgtttga atggcaataa atttttcgag taataaatcg aattgatctt ttttgtaatt
      421 cataagttga cggtcaaaat cactttcgat tagaactttt agatatttat ttcgactcca
      481 ttttttgccc gttttggttg tgagttcctg acaacgttta tctattcttg caataatttt
      541 gggattgata tttcgaacta aaatgtccat ggaaattttc ctttcgtatc agcgtataat
      601 ttatgtatat catacaaata tagtatttgt atagctattt ttcgccaaaa cactatacat
      661 agcctagcca caatggcaaa aaagtgatag caaatttttt tctgagaaac gtgataggca
      721 cagcctatct agcacctttt gtaagaaagt gataccacgt ttccttatgc tctttcaaaa
      781 ttcagaaaaa tcagctgtaa aacgagtgca gtccgagtaa gattcaacga aaaaacgggg
      841 attggggcgc caccaaaata tcgcaacatg ggtccatgtt gctctgctcg caaaagcaaa
      901 aagaagaata aaaccagcaa tttaaattaa taaaaattgc tggttttatt gccagaattg
      961 ccaccatttt ttctccgtct ttgattgttt tttctgtcat aaaatctaaa aacccctcaa
     1021 tctttgaata agttttgaag attgtctaat aagtcttttt ttagttctga aggaatatcg
     1081 gaatttatct ctgatttttg tgcattattt tctttatttt gggtattttg ttctctttct
     1141 aacttttcta actgttgtaa aagttccaat tttcgctttt gttctttgat tttttcttgt
     1201 aatgaagtag gtgctttttc agattgaact tgttggttag ctagttctaa tgctttgttt
     1261 tgttcttctt ttgttggggc atgtttgtta aagtcgtctg atttttttat ctcaggttta
     1321 aatgtaaatt gatagccaat gacaggcttt ccccgtcctt ttccgtattt ctttttgatt
     1381 gttaaacctt taaagattgc agttagttct tgtcgtattg gattgaacac acgttgatca
     1441 atcgtacctt ggttgtaact tttaggaata tcaagtaatt catgaaagtc ttcttttgaa
     1501 aaataagcga aacctttggt tctgtattgt tttagtaatc gaaacattgt ttttgaataa
     1561 ctgctttcta attcattgaa ttgttggaga gaatagcgca cccattcttc aagattatta
     1621 agtaagggta aggctttttc atgaacttgt atttcagcaa aaggaatatc agattttccg
     1681 tcgattttaa attgattaaa taaaacaaaa cgttcacgtt gcaaaccgtc agcacttctt
     1741 ctaccaaaac gtaaatccat taatttgtca tacgttgttt ctaaatcatc tatgaatcga
     1801 acatttgcag ttgctttata attgcttaaa tcttttaatt gctcgaatgt aaattgtact
     1861 tttttatcac ctacatcacg cattcttgat acgatagaaa agaacaaatt catttcgact
     1921 ggtgtaaatt ttcgcaacgg aattgtattt aattcatgat gatactttac gatttcatta
     1981 gacatattta aaatagctcc tttcttactc gcacaagaga tacacgttat gaaatacaaa
     2041 atgcctattt atctacatct tatattaaca tcgtcgatgt actaattcaa tgtacaaaca
     2101 gacaaaacga tgtagataaa cagacaaaac gatgtagata aacagacaaa acgatgtaga
     2161 taaacagaca aaacgatgta gataaaagcg tcattccctt gggagagtaa ggatatagcc
     2221 gacggtaaag aggtttaaaa agaggtttaa aaagagatta taaaagaggg ggacaaaatt
     2281 tcccgcctaa aaaaccactt cttttttctc caaacagatt cgcgcatttt tctcaactgg
     2341 gaatcttact ccctacagtc gcttaaaaag gtgtgatcgc tctgctctca cattatactg
     2401 cgcagtcgct tccggctcct ttgctaaaaa tattcataag gaactctttg agaagctatg
     2461 agccttcgtt tgagacgttt taagacagtc ttagtataaa tcccttatct tgttcttaaa
     2521 gcgccttaca ggcggtcagg gcaagcccta acaaccctaa agttgtctga atgccctcta
     2581 aaattcgatt taagcactct taaacctatt cttatatatt tatatactta gacgtttcaa
     2641 acgtcttaaa acgcaaatta gagcttgata aagatatttg ctcgtgtgcc gactttttta
     2701 aaagtctagg cacacgccat gacaaagctg aaataaactg cttcagcttt gtcatggcgg
     2761 attcgctcac ttgaaaaagt gttcatttcg ctcgattatt agcatatatt ttttaacttt
     2821 ttttgctagc caaattttgc attagttcgc tcataaatcc caccctttca gtacgtccta
     2881 cggacgtatc gttcgtaatg tgaaagtacc tttaaaggta ctttctacaa aactcactaa
     2941 agggtttggc aagctctata tttgctagcc aaatacctac gtatttggtg caaataaccg
     3001 cttgtcaaag gggcgcaagc tcccccttga atcccccctc aaaacccacg ctccacaata
     3061 cacaaaaggc tagcaaatgc tagccacttt ttgtaaagtt tcgcttgttt caccttcggt
     3121 gaaattgttg ctttgcaacg atcaggtaga gctacatttt ttttcgttgt tcaaatattc
     3181 ttacgccatt tactattaaa atcgtaggta ttccaaagta aagaaaaaat tgattagcag
     3241 gtaatacaaa atataaaata ataccagcaa taattaccaa acttatagta agttttgtta
     3301 atttcatgaa gtatgccacc ttatcttata tagatatcaa atagattaat atgccgattg
     3361 tatgagcaac tatggaatac catatatttt ttgtttgtat ataaacaaaa cttaaaataa
     3421 taccaataaa gaaaatactt aggcctcccc atgaacaata gaaaacagaa aagaagaaat
     3481 tagtattgaa atatattgtt gttttttgtt tgcttgagga tcaatcatac aaaatcgaaa
     3541 aattccttct tctaaaaaag ctccataagt agcagcattt aaaaagaata aaaggagaga
     3601 accatttact tcattaggat ttataccttc tgaaaaagat gtatttagaa tattgattag
     3661 gaataaaaca gagaatgaaa aaaactatat ttaaagagtt tttttagatt tacttctttg
     3721 aattcacgaa tgaaggtaga atgaaatagt ataattatta gcactaaaaa aatatatcac
     3781 tatgttcaaa taaaaaatat atatttgacg gtactcctat actcctaatg atgggatagc
     3841 aaaggtcaaa accaagtaag caatactaat tatccatttt attgtcatta ggcaggaata
     3901 taatcaatat attgtgtatt tgctgtagaa gtttgagtac gaatatattt attatctacc
     3961 ttacgataaa ctctgtaggt tacagttatt cttgcatttt gacgatattt tttaaatttt
     4021 ccacttgcag aagtgtttct ggctatttcg attttcatcc caaatacatt tgcactgaca
     4081 gataactttg tacctcgact atgagtttct atacctaaat aagtacggct tcctttagca
     4141 attgaccagc tttgaggttt agaccaagtc cttgtttgaa taactggttc gtctaacaca
     4201 tagtagtact catcatcaga ataagtggct atatcagaaa tgttgttctc attagcgaat
     4261 accgctccgc taggagtaat agcagaacca aaagttaaaa tagaagtaga taataaaaag
     4321 gttttaaata actgtttttt catcaaaatc acctcgaata taagatactt aagaaaacgc
     4381 tttctttata taattagaat aatcaggaaa tttaatgttg tcaagagggt ttattttata
     4441 aaagctgtta tattaaaggt tagtagacat aatgtacata ctaccaagtt gcccctctac
     4501 caatggatag agacgctcac tttgtgattt ctaattggtc tagcttaaac acttcagcca
     4561 atgcttgatt ttatgcactt ttcttttttc atttctttgt gcattttgac cggtcatttt
     4621 ttagagattt gttatttttg tgagcatttg atatgctctt tttatacctc aatctcttct
     4681 tttctaatgt taatgaatag gataatctca tttttagctg ctcgtgagag cagtcttttt
     4741 ttgtttcagg gtacgtccta ttctttggat ttccgcgcta ttaaataacc gatacttggg
     4801 gattggggta gcgccccaaa atacaaaaag aagaagttgc ataagcggat ttttcttttt
     4861 tttcgcaagt tggtaccaac ttgcaaatct tgctggaaga ctgatcccta aaaagaaatc
     4921 gaggaatttt tttagggatc ggtttctttt tttatctttt tagaaagttt gttgggtcaa
     4981 aatttccaga agtaaaggtt tgattggttt ctttttgacg atttttgggt tgatttcgtc
     5041 gggaaggatt gagttgattt tcaatttttt ct
//
