LOCUS       AP026621               28796 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA96 plasmid pAA96_3 DNA, complete sequence.
ACCESSION   AP026621
VERSION     AP026621.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466810
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 28796)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..28796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA96"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA96_3"
                     /geo_loc_name="Japan"
                     /collection_date="2011"
     gene            complement(493..1230)
                     /gene="ermB"
                     /locus_tag="EfmAA96_34920"
     ARG             complement(494..1230)
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.78"
                     /coverage="98.79"
     gene            complement(1981..2775)
                     /gene="aphA"
                     /locus_tag="EfmAA96_34930"
     CDS             complement(1981..2775)
                     /gene="aphA"
                     /locus_tag="EfmAA96_34930"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001096887.1"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-IIIa"
                     /protein_id="BDP75708.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /name="aphA"
     gene            3364..3636
                     /locus_tag="EfmAA96_34940"
     CDS             3364..3636
                     /locus_tag="EfmAA96_34940"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     3760,3597"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75709.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRAC
                     RSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHIGYAPKKATLYL"
                     /name="hypothetical protein"
     gene            3781..4038
                     /locus_tag="EfmAA96_34950"
     CDS             3781..4038
                     /locus_tag="EfmAA96_34950"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     3597,3997,3766; possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75710.1"
                     /translation="MTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFI
                     CADGETGKLIDVLPTRKLPRLTSYFLGLYQSRRSRILGAA"
                     /name="hypothetical protein"
     gene            4025..4291
                     /locus_tag="EfmAA96_34960"
     CDS             4025..4291
                     /locus_tag="EfmAA96_34960"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     3997,3766; possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75711.1"
                     /translation="MVPHERRLAQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMN
                     ELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPK"
                     /name="hypothetical protein"
     gene            4313..4657
                     /locus_tag="EfmAA96_34970"
     CDS             4313..4657
                     /locus_tag="EfmAA96_34970"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748789.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP75712.1"
                     /translation="MMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELPET
                     LDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMRIRI
                     FLINQLINVR"
                     /name="transposase"
     gene            4758..5666
                     /locus_tag="EfmAA96_34980"
     CDS             4758..5666
                     /locus_tag="EfmAA96_34980"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002295743.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="BDP75713.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQL
                     KQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKII
                     RYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHM
                     IVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKLALKGITIS
                     VPPRKNMDKSEKLDHSLLGKQRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAY
                     SVLLSRAQRRFGGTLRYSLGY"
                     /name="IS982 family transposase"
     gene            complement(5898..6578)
                     /locus_tag="EfmAA96_34990"
     CDS             complement(5898..6578)
                     /locus_tag="EfmAA96_34990"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001015311.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDP75714.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6 family transposase"
     gene            6787..6999
                     /locus_tag="EfmAA96_35000"
     CDS             6787..6999
                     /locus_tag="EfmAA96_35000"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75715.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAAA
                     VVITAVAKASDSILAWLKFKKEQKK"
                     /name="hypothetical protein"
     gene            7157..7675
                     /locus_tag="EfmAA96_35010"
     CDS             7157..7675
                     /locus_tag="EfmAA96_35010"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75716.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSEI
                     FENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEFKL
                     ALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLSY
                     EMISKVLSL"
                     /name="hypothetical protein"
     gene            7878..8222
                     /locus_tag="EfmAA96_35020"
     CDS             7878..8222
                     /locus_tag="EfmAA96_35020"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     7744,7813"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75717.1"
                     /translation="MEKCKATDAIFSFLRKSDEIITRENIDLENEISKLESIFSDINSL
                     KYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSIISQNMDKLNG
                     KVLFLKIWIN"
                     /name="hypothetical protein"
     gene            8501..9124
                     /locus_tag="EfmAA96_35030"
     CDS             8501..9124
                     /locus_tag="EfmAA96_35030"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289893.1"
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="BDP75718.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVSG
                     KNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLPSF
                     NGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYHANA
                     TGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
                     /name="resolvase"
     gene            9220..9900
                     /locus_tag="EfmAA96_35040"
     CDS             9220..9900
                     /locus_tag="EfmAA96_35040"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001015311.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDP75719.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6 family transposase"
     gene            10098..10703
                     /gene="fic"
                     /locus_tag="EfmAA96_35050"
     CDS             10098..10703
                     /gene="fic"
                     /locus_tag="EfmAA96_35050"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000599739.1"
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="BDP75720.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="fic"
     gene            10719..11291
                     /locus_tag="EfmAA96_35060"
     CDS             10719..11291
                     /locus_tag="EfmAA96_35060"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000170424.1"
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="BDP75721.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="resolvase"
     gene            complement(11764..12186)
                     /locus_tag="EfmAA96_35070"
     CDS             complement(11764..12186)
                     /locus_tag="EfmAA96_35070"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75722.1"
                     /translation="MDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDL
                     GSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANR
                     ALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
                     /name="hypothetical protein"
     gene            complement(12437..12700)
                     /locus_tag="EfmAA96_35080"
     CDS             complement(12437..12700)
                     /locus_tag="EfmAA96_35080"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287855.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP75723.1"
                     /translation="MSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVN
                     WWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="transposase"
     gene            complement(12973..13272)
                     /locus_tag="EfmAA96_35090"
     CDS             complement(12973..13272)
                     /locus_tag="EfmAA96_35090"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     12809,12978"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75724.1"
                     /translation="MCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKI
                     KKALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKL"
                     /name="hypothetical protein"
     gene            complement(13308..13598)
                     /locus_tag="EfmAA96_35100"
     CDS             complement(13308..13598)
                     /locus_tag="EfmAA96_35100"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002322074.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP75725.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGRRDK"
                     /name="transposase"
     gene            complement(13639..13953)
                     /locus_tag="EfmAA96_35110"
     CDS             complement(13639..13953)
                     /locus_tag="EfmAA96_35110"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000882571.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP75726.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQR
                     YGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN
                     "
                     /name="transposase"
     gene            complement(14004..14258)
                     /locus_tag="EfmAA96_35120"
     CDS             complement(14004..14258)
                     /locus_tag="EfmAA96_35120"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000199136.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP75727.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            complement(14451..14726)
                     /gene="prgO"
                     /locus_tag="EfmAA96_35130"
     CDS             complement(14451..14726)
                     /gene="prgO"
                     /locus_tag="EfmAA96_35130"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001196543.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75728.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(14698..15651)
                     /gene="parA"
                     /locus_tag="EfmAA96_35140"
     CDS             complement(14698..15651)
                     /gene="parA"
                     /locus_tag="EfmAA96_35140"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000429439.1"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning ATPase"
                     /protein_id="BDP75729.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="parA"
     gene            16263..17723
                     /gene="repR"
                     /locus_tag="EfmAA96_35150"
     CDS             16263..17723
                     /gene="repR"
                     /locus_tag="EfmAA96_35150"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000947691.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="BDP75730.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSNPLEPS"
                     /name="repR"
     gene            17892..18188
                     /gene="prgN"
                     /locus_tag="EfmAA96_35160"
     CDS             17892..18188
                     /gene="prgN"
                     /locus_tag="EfmAA96_35160"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000053907.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="BDP75731.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="prgN"
     gene            complement(18212..19171)
                     /locus_tag="EfmAA96_35170"
     CDS             complement(18212..19171)
                     /locus_tag="EfmAA96_35170"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287225.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="BDP75732.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLFF
                     KQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCS
                     ARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSITF
                     DCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDESF
                     IQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
                     /name="IS30 family transposase"
     gene            19354..20205
                     /locus_tag="EfmAA96_35180"
     CDS             19354..20205
                     /locus_tag="EfmAA96_35180"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287227.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75733.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            20226..20438
                     /locus_tag="EfmAA96_35190"
     CDS             20226..20438
                     /locus_tag="EfmAA96_35190"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 20432"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75734.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            20871..21281
                     /locus_tag="EfmAA96_35200"
     CDS             20871..21281
                     /locus_tag="EfmAA96_35200"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75735.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDWLRKKRDTQA
                     AYAFLKRLVKQFDEPKVVVTDKAPLLQVPLRN"
                     /name="hypothetical protein"
     gene            21363..21551
                     /locus_tag="EfmAA96_35210"
     CDS             21363..21551
                     /locus_tag="EfmAA96_35210"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75736.1"
                     /translation="MIEQDYRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTL
                     FGFSVCTEIKVLLGIPA"
                     /name="hypothetical protein"
     gene            complement(21574..22494)
                     /gene="repU"
                     /locus_tag="EfmAA96_35220"
     CDS             complement(21574..22494)
                     /gene="repU"
                     /locus_tag="EfmAA96_35220"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002322009.1"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="BDP75737.1"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDGFCCKVLNKE"
                     /name="repU"
     gene            complement(22718..23980)
                     /locus_tag="EfmAA96_35230"
     CDS             complement(22718..23980)
                     /locus_tag="EfmAA96_35230"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000119405.1"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="BDP75738.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENYD
                     LKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRFFE
                     ESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQDKF
                     PEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYSDKVK
                     SDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTAARDND
                     RLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEENKSLKSK
                     ISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHDLEPKKNE
                     FELTHNREVKKERSRDQGMSL"
                     /name="Plasmid recombination enzyme type 2"
     gene            complement(24544..25920)
                     /gene="tetL"
                     /locus_tag="EfmAA96_35240"
     CDS             complement(24544..25920)
                     /gene="tetL"
                     /locus_tag="EfmAA96_35240"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001574277.1"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="BDP75739.1"
                     /translation="MNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
                     /name="tetL"
     gene            complement(26114..26482)
                     /locus_tag="EfmAA96_35250"
     CDS             complement(26114..26482)
                     /locus_tag="EfmAA96_35250"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75740.1"
                     /translation="MLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYC
                     ANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQP
                     RRPNSRIDKVRYMFNKIT"
                     /name="hypothetical protein"
     gene            26614..27294
                     /locus_tag="EfmAA96_35260"
     CDS             26614..27294
                     /locus_tag="EfmAA96_35260"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001015311.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDP75741.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6 family transposase"
     gene            27350..27607
                     /locus_tag="EfmAA96_35270"
     CDS             27350..27607
                     /locus_tag="EfmAA96_35270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP75742.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
                     /name="hypothetical protein"
     gene            27699..27914
                     /locus_tag="EfmAA96_35280"
     CDS             27699..27914
                     /locus_tag="EfmAA96_35280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001835296.1"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="BDP75743.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETA
                     KNGGNVKEVMDQALEEYIRKYLPDKL"
                     /name="peptide-binding protein"
     gene            27931..28203
                     /locus_tag="EfmAA96_35290"
     CDS             27931..28203
                     /locus_tag="EfmAA96_35290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000301765.1"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="BDP75744.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVN
                     LLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
                     /name="antitoxin"
     ARG             complement(24548..25920)
                     /name="tet(L)"
                     /gene="tet(L)"
                     /gene_name="tetracycline efflux MFS transporter Tet(L)"
                     /tool="amrfinderplus"
                     /drug_class="TETRACYCLINE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(1985..2775)
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(497..1230)
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="99.59"
                     /coverage="100.0"
     ARG             complement(26115..26482)
                     /name="tet(M)"
                     /gene="tet(M)"
                     /gene_name="tetracycline-resistant ribosomal protection
                     protein"
                     /tool="rgi"
                     /drug_class="tetracycline antibiotic"
                     /identity="100.0"
                     /coverage="19.09"
     Typing          16264..17757
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.799"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(21607..22494)
                     /name="rep_cluster_1018"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          complement(22722..23980)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.19"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 attcaagttt ggaaacacct tctattagtc caaaagaaac cttagaaaaa gaattgaatc
       61 gtaaagtatc agggaaagaa attcaaccga ctttagaacg aatagagcaa aaaatggttc
      121 taaataaaca ccaagagaca cctgaattta aagcaattca acaaaaattg gaaagcttgc
      181 agccacctac accaccaata cccaaaacac ctaaacttcc aggtatttaa accttaaaaa
      241 aggaaaagag tagttaccaa aaaacggtaa ctactctttt tttataaaaa catttcctca
      301 tgttggggtc ccgagcgcct acgaggaatt tgtatcgata agaaatagat ttaaaaattt
      361 cgctgttttt ttctaatttc attttgaggt gaattaattg gtagttatta gaggtgccct
      421 atacctaata atttatctac attcccttta gtaacgtgta actttccaaa tttacaaaag
      481 cgactcatag aattatttcc tcccgttaaa taatagataa ctattaaaaa tagacaatac
      541 ttgctcataa gtaacggtac ttaaattgtt tactttggcg tgtttcattg cttgatgaaa
      601 ctgattttta gtaaacagtt gacgatattc tcgattgacc cattttgaaa caaagtacgt
      661 atatagcttc caatatttat ctggaacatc tgtggtatgg cgggtaagtt ttattaagac
      721 actgtttact tttggtttag gatgaaagca ttccgctggc agcttaagca attgctgaat
      781 cgagacttga gtgtgcaaga gcaaccctag tgttcggtga atatccaagg tacgcttgta
      841 gaatccttct tcaacaatca gatagatgtc agacgcacgg ctttcaaaaa ccactttttt
      901 aataatttgt gtgcttaaat ggtaaggaat attcccaaca attttatacc tctgtttgtt
      961 agggaattga aactgtagaa tatcttggtg aattaaagtg acacgagtat tcagttttaa
     1021 tttttctgac gataagttga atagatgact gtctaattca atagacgtta cctgtttact
     1081 tattttagcc agtttcgtcg ttaaatgccc tttacctgtt ccaatttcgt aaacggtatc
     1141 ggtttctttt aaattcaatt gttttattat ttggttgagt actttttcac tcgttaaaaa
     1201 gttttgagaa tattttatat ttttgttcat gttatcactc ctgaagtgat tacatctata
     1261 aacaaataca gaagttaaac gatttgtttg taattttagt tatctgttta aaaagtcata
     1321 agattagtca ctggtaggaa ttaatctaac gtatttattt atctgcgtaa tcactgtttt
     1381 tagtctgttt caaaacagta gatgttttat ctacataacg catttggaat accaacatga
     1441 cgaatccctc cttcttaatt acaaattttt agcatctaat ttaacttcaa ttcctattat
     1501 acaaaatttt aagataatgc actatcaaca cactcttaag tttgcttcta agtcttattt
     1561 ccataacttt agggttaacc atacgcaaga ccaatcactc tcggacaata ctcatgattt
     1621 taatgataaa atccatgtta aacccataga taagaaatac acctgcaata accgttacct
     1681 gtttgtgcca atttaaaaat gcaccacttt tttataatta aaacggttga aaactgtacc
     1741 actaataact cacaatagag agatgtcacc gtcaagttaa atgtacaaaa taacagcgaa
     1801 atttttaaat ctatttctta tcgatacaaa ttcctcgtag gcgctcggga cccctatcta
     1861 gcgaactttt agaaaagata taaaacatca gagtatggac agttgcggat gtacttcaga
     1921 aaagattaga taagtctaaa agcttgtagt taaagctttt tagacatcta aatctaggta
     1981 ctaaaacaat tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc
     2041 cagtaagtca aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg
     2101 gacgcagaag gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa
     2161 gccacttact ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa
     2221 gacaagttcc tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt
     2281 aaatggagtg tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa
     2341 gtaatccaat tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc
     2401 gatggagtga aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg
     2461 ttcatcttca tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct
     2521 ccagccatca tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca
     2581 tagcatcatg tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt
     2641 catttttaaa tataggtttt cattttctcc caccagctta tataccttag caggagacat
     2701 tccttccgta tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat
     2761 tctcatttta gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa
     2821 gaagctaatt ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa
     2881 taccagaaaa cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg
     2941 gagccgattt tgaaaccaca attatgatag aatttacaag ctataaggtt attgtcctgg
     3001 gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga tatattgata
     3061 agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa
     3121 aagataaccc gtggtgctag ttaatttttt cctagaggaa aatgtctaaa catgttggta
     3181 aatcaacaaa aaaacgcata tagtagtctc aaaactccca agaaatgggc tctataattt
     3241 atgggactga tgaatcagaa ttgattcttc agccccattt tcattttagg tattaaaaaa
     3301 catatcgttc tctagtatga ttaaatcacc acaaaataaa caactgagag gacgatacgc
     3361 tatatgaatg attctatcaa aaaaatgctg agaataacag agaaagattt gatgattaca
     3421 gaggtctctt acgagaccct tcagaagaaa aagacgttgg tcgtcgatgc tgttctctcg
     3481 cctactcctc gtgcttgtag aagttgtggt tctactgtgg tagatggaaa cgggaaagca
     3541 attatagtga aaaatggaaa aaaggaaacg attgtccgtt ttgaacaata caatcatatt
     3601 ggttatgcgc ctaaaaaagc aacgctatac ctgtaaaaac tgtcgcaccc attggacggc
     3661 tcaaagttat tttgtccaac ccagacattc aatcgcaaat catgttagat ataaaattgc
     3721 ttctttactg actgaaaaag tatctttatc ttttattgcg aaaaaagctg tcaggtatct
     3781 ttgaccaccg ttattcgtac attgaaagag tttaaaagct atttaccaaa gcaatctaag
     3841 aagatcctcc ccagagtatt gatggttgac gaatttcgtt cgcatgcttc catagaagat
     3901 aagatgagct ttatttgcgc ggatggcgaa acaggaaaat taatagatgt tttgcctacg
     3961 cgtaaattac ctcgattaac aagctatttc ttagggttgt accaatccag aagaagtaga
     4021 attcttggtg ccgcatgaac gccgcctagc tcagctcacc aaacgtgttc tgccaaatgc
     4081 gaaagtcgtg attgatcggt ttcatattgt caaacacatg aatcaagcgt tcaatgagtt
     4141 gcgtatccgt gaaatgaatg aacttcgtaa agccggacag aaaagccagg cagaaaaact
     4201 gaaaaagaac tggcgctttt tgctaaaaaa tcgtgcaaac atcaaccatt atgaatacaa
     4261 aacatggaaa agtttccgag caccaaaata atacccattt cttactgaag caatgatgat
     4321 tgatcgattg cttgagtttt ctccgcccct aaaggaggcg tatccctttt ttcatgaatt
     4381 agttgaagcc tttcgagaca aagaccctga cttatttttc tccttattgg cagaacttcc
     4441 cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac cttctgacct atgaagaagg
     4501 catcaccaac gcaatgatct atccttattc caatggaaaa atagaagcga agaataccca
     4561 cataaagaca atgaaacgag tatcctacgg atttaaatca tttgagaaca tgagaattag
     4621 aatctttttg atcaatcaat taatcaatgt aagataacaa aaaatctgag ccagaatgtg
     4681 gttcattctg actcagattt gattttgcac aactcatcag tccttattga caaagagcca
     4741 agaaatgagg actacatatg cgttcatcaa aatatactga acattataca gatactttta
     4801 taacattcaa ggaaattatg agaactgttt ctaacatata ccacaattgt gtaccagata
     4861 agattaaaaa ccgaagaaat actgatcaac tgaagcaacg cgatacagtg attattgctt
     4921 gtgttatatg gggtataatt aatggctata ctagccaaag agccacgtat agagcggttt
     4981 gttccgtctt atttcctaat ggtgactttc ctagtaggag tcgattcact cgcttaagtt
     5041 caaacttagc ttacactatc aagattattc gatacttttt catcaagaag ctcaccaaag
     5101 gtgaactagt cggaattata gatagttttc ctagtccatt atgtaaacca gttagaaata
     5161 gacaagcaaa actattgaat caaatagcca aagttggcta taactcaaca aaaaaatctt
     5221 atttttatgg tcttaaaatt catatgatcg ttactaagac tggctttccg attacttact
     5281 caatcacgaa tccaggtgtc catgacgtga aagtgttaga gactttatcg gaagaagcga
     5341 atcttcccaa tatcctaggg gacaaaggat atattagcca taaaatccat gaaaagcttg
     5401 cccttaaggg cattactata tccgttcctc cacgtaaaaa catggataaa tcagaaaaac
     5461 tagaccacag cttacttgga aaacaaagaa aaacagttga gactgttttt tcttccttag
     5521 aaaaattagg ttgtcaaaat ttcaactcac gttctgttaa agggctagag agtagatttg
     5581 aaagcatatt actggcttac agtgttctat taagtcgagc acaacgacgt tttggaggaa
     5641 ctttgagata ttctttagga tattaaaatt aactagcacc acgggttaaa agatatatta
     5701 tcttatcagt attgtcaata tattcaaggc aatctgcctc ctcatcctct tcatcctctt
     5761 cgtcttggta gctttttaaa tatggcgctt catagagtaa ttctgtaaag gtccaattct
     5821 cgttttcata cctcggtata atcggttctg ttgcaaagtt ttaaataaag aataaaatcc
     5881 cttacggtat ctatgattta agctgggatt cccaataata ccttgatttc agtacagacc
     5941 gaaaacccga agagagtgcc ttcttttcgg gttttcttat ataatcctcg aatggcttcc
     6001 atgcctttaa tcgtggtaga ggcagtgcgt aaacttcgat agaatttatt gcgtctcttt
     6061 actggacgat ggtcttgttc aatcaaatta ttcaggtatt taatggtacg atgttctgtc
     6121 ccttgataaa agccgtattc ttttagtttc ttaaaggcac ttgtaataga gggggcttta
     6181 tctgtgacta caaccttcgg ttcatcaaac tgcttcacta accgcttaag aaaagcatag
     6241 gctgcttgtg tgtcccgttt tttacgtaac caaatatcca aggttaaacc atctgcatcg
     6301 atggctcgat acaaataatg ccattttcct ttaattttga tgtacgtttc atccattttc
     6361 catgaataaa aggatttttt atttttcttt ttccaaattt gatagagtag tttgccatat
     6421 tcttgcaccc aacgataaat cgtcgtatga gaaacgttaa tgccacgatc atataagatt
     6481 tcttgaactt cacgatagct aaggttataa cgaagatagt agcccacggc tacaataatc
     6541 acatcctgct gaaattgctt tcctttaaaa tgattcatcg tcattcctcc tgctatcttt
     6601 ttctattatt ctaccttatt tgatagtaga tttaaaactt tgcaacagaa ccctaaaaac
     6661 actatatatt ccgaaaagtg agtatctcca ttcataaaat aatcaacata tttaaaaaat
     6721 ctaattagct tttttagtca atctaaagtt ctttattcta tgatataatg ttactgtaga
     6781 aagtttgtgg cggtttggca aatccaattg aaggagcgtg gtgcctatgg gcttattagc
     6841 ttatcggacg tactctgtag aaaggaaatg cctgatttgt tctcattgga tcaaatcatt
     6901 gctattatcg gagcagcagc agtagttatt actgccgtag cgaaagctag cgattcgatt
     6961 ttagcttggt taaagttcaa aaaagaacaa aaaaaataga gccgtctcaa cttttggcga
     7021 ggtagacgag ctctaaagct ttatttacat gccatccgct ttgaacggac tacactgggc
     7081 atgtgacagc atgccctttc atttatatta tcattctttt atataatgaa agtaaataaa
     7141 tcatggagga taaaatatgg ataaatttaa tagattaaca ttaataaatc agtttgaaat
     7201 tttaaaagct ttaaatccta acgaagaaaa atactatgct gaaaaaatag aaatactaac
     7261 agaaggttac gaatatcact atagtgaaat atttgaaaat atttctgatc cattgccaga
     7321 agaagattcg cggtttgttc tagacatatt aaatatgtat agagatatta cttttagtaa
     7381 aaataaatta aaagatataa attcaattga taattattat atacaattta aaggttttga
     7441 ctttaatagc tctaccgaat ttaagttagc actttatgca caatttttta ttgaaaaatt
     7501 aaataggttt caagaaatag ttgaagatag tgattttaac acttttaata gccataaacc
     7561 gatgagaggt acttatataa aatttctcga aaattataat gatttaaaat ctaccaataa
     7621 ttatcaattt ggaaacttgt cttatgagat gataagtaaa gttctaagtt tgtgatgata
     7681 aatgaaatta aatagtataa atgatttatt tgctattata ggtgttattg ctaacatact
     7741 ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact cagtatagaa
     7801 caaatgaaaa agacattgaa actgatttta aaaatgacca taaagaaaga agaaaaattg
     7861 aagaatatac ttctttaatg gaaaagtgta aagcaactga tgcaattttt agctttttaa
     7921 gaaaaagtga tgagataatc acacgtgaaa atatagattt agaaaatgag attagtaaac
     7981 ttgaatctat cttttccgat ataaattctc ttaagtattt aatcgtcgaa gaaaattcgg
     8041 atagcttttt agaatttgaa aaatatctaa cagatcttat agataataga aataatgaat
     8101 taatgagaca aaacattgca agattcctta ctaaattagt ttctgaatgg aaaagtatta
     8161 tttctcaaaa tatggataaa ttaaatggaa aagtattatt tctcaaaata tggataaatt
     8221 gagatattaa ggatgttagt tttaaaataa actaatgtcc ttatttttgt gttcacattt
     8281 ctgttaccta agaaatactt aaaggaggtg atgtagccag caacgactac aacttgaacc
     8341 cgctatctac agtttatttg ttgtttcttt tgggtatact ctaatggtac aaatccatat
     8401 ttgcttaaag cgttgatttt tcagcggttt ttctttattg caaagtgtac cctaagacct
     8461 gtatactaat agtaaataga ttggatagga atgatggcaa atgtatttag cttatttaag
     8521 agtttcttcc gacgatcaaa gtttagctcg gcaacataag ctaattgaaa attggtcagt
     8581 caaaaataat attgctgaag acgatttaaa aattttcgag gaaaaagtct ctggaaaaaa
     8641 tattgaaaac agaccagagc tgcaagagct tatttctttc atacgtgaac gagatacgat
     8701 tattattcct agtcttgatc gattagcaag aaatagtaaa gatacaaaag acttacttca
     8761 attgtttcgt agtaaaggtg cttctattga aatattagac ctaccgtcgt ttaatggggt
     8821 aactgaccct gcattaagag acttgctaac aaatttagtc atagaagtgt ttagctatgt
     8881 tgctgaaaac gaacggaaaa aaatcaaaga gcgtcaaaaa caaggcattg aaattgcaaa
     8941 gaaaaatggt cgttatactg gtaaaaagat tcaataccat gccaatgcaa caggaaaaaa
     9001 caaattgatt tacgatgaaa ttgttgatgg tctgaagaaa aaagaaacta tttctaaaat
     9061 agcaaagaac gcaggagtta caagaaaaac cgtttatcga gtaaaaagag atttaggaat
     9121 gtaatatgtt ccagttagaa agaagggttc tgttgcaaag ttttaaatct actatcaaat
     9181 aaggtagaat aatagaaaaa gatagcagga ggaatgacga tgaatcattt taaaggaaag
     9241 caatttcagc aggatgtgat tattgtagcc gtgggctact atcttcgtta taaccttagc
     9301 tatcgtgaag ttcaagaaat cttatatgat cgtggcatta acgtttctca tacgacgatt
     9361 tatcgttggg tgcaagaata tggcaaacta ctctatcaaa tttggaaaaa gaaaaataaa
     9421 aaatcctttt attcatggaa aatggatgaa acgtacatca aaattaaagg aaaatggcat
     9481 tatttgtatc gagccatcga tgcagatggt ttaaccttgg atatttggtt acgtaaaaaa
     9541 cgggacacac aagcagccta tgcttttctt aagcggttag tgaagcagtt tgatgaaccg
     9601 aaggttgtag tcacagataa agccccctct attacaagtg cctttaagaa actaaaagaa
     9661 tacggctttt atcaagggac agaacatcgt accattaaat acctgaataa tttgattgaa
     9721 caagaccatc gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct
     9781 accacgatta aaggcatgga agccattcga ggattatata agaaaacccg aaaagaaggc
     9841 actctcttcg ggttttcggt ctgtactgaa atcaaggtat tattgggaat cccagcttaa
     9901 atcatagata ccgtaaggga ttttattctt tatttaaaac tttgcaacag aaccaactag
     9961 actgtgcaaa atagagacac ttgaaaaatc tatcgggggt cagaaactta atagtttttg
    10021 acacataaac aaaatcgcga tataatgggg ttgagagtgt ttcataaact tatgtcataa
    10081 agttaaggag atttcgcatg attttagaga ataaattggg attaaccaat caagttgagt
    10141 tggctaaggt tgaagaaaaa ctcagtaaac aaaaagcgaa agaattgtac gattgtggaa
    10201 aaataaatga aatcgaagtt ggtacattta aaggtttatc tgaaattcat gaatttctat
    10261 tttcggatat ttacgatttt gccggaaaaa tccgatcggt caatattgca aaaggaaatt
    10321 ttcgatttgc accagttatg tatttagaac attcgttaca gcatattgat cagatgccac
    10381 aaacttcttt tgaagagatt atcaagaaat atgtggagat gaatatcgct cacccatttc
    10441 gagaaggaaa tggccgaagt actagaattt ggctggatct cattttaaag gaagaattgc
    10501 aaaaagttgt agattggaac ttaatcgata aagcagacta tctttctgca atggagcgaa
    10561 gtcccataaa cgatttggaa atacgctatc taatttccaa tgctctgact gataaaattg
    10621 acgatcgcga gttatatatg aaaggaatcg atgtcagcta tttctatgaa gggtattctg
    10681 agtatacgat agatgatttg taaaagtagg tggaccaaat gacgaaatat ggatatgcac
    10741 gtgtctcaac cgcttctcaa agtacaaaag aacaaattca acagttgctt gagaactgcg
    10801 tagagaagaa gaatattttt tcagaaaaat ttacgggtac aaaacgggat cggccaaaat
    10861 tcaatcagct gttggctggt ctgaagcctg gagatgaaat agttgttacg aagcttgatc
    10921 gatttgctcg taacacgaga gaagcattag agatcattga acctttactc gatgataacg
    10981 taacgatcaa ggttttgaat ctaggaacga ttgaaaatac gtccatgggg cgaatggtga
    11041 cacggacgtt gttgagtgta gcagaaatgg aacgtgatat gattgtggaa cgtacccaag
    11101 aaggaaaaat gtttgcaaga aagaataatc ctaatttcag agaaggacgt ccgaaagcaa
    11161 caatcacacc gaagaagagg cacgcatatg aactgcttac ttcaggtaaa tcgtataagg
    11221 aagttgaagc tataaccggt ttcagtcgca gtacgctttt tagaataaag aagaaaattg
    11281 aagaaagtta aaaaagaagt ctcagcgaac cagacaggta aatacttaat gcgttctaga
    11341 tatgagtcta ttagcatgtt gtgaaatata atattttttt atttacttga tttctgctca
    11401 aaaaatgtgt atggtaactg taaaaggtaa agtaattaaa ggtatgctag ccacgcttac
    11461 tatattagga taccatcata tatttataat gaacgatttg ttcgtctacc aaacaacaca
    11521 tatcgttcag cgtaagtcac gtttaggaac gtggcttttt tagtgttatt atcgataaat
    11581 aatctattga ctctattgat tttatctcca tatccctacg gtgttgattg tctttagatc
    11641 tttcatttaa gaaccaaata actatcataa aaatcagcac tgctgctaat ataataaaca
    11701 gcattcctat tgtgatatta ctggatttga gtcaatatct tggacacatt ttagtagaga
    11761 cggttaagcc attttttctt ttgctaattc ttcaaattcc tgtggggtta gatagtgaat
    11821 cgagctatgg attcgatttc ggttataaaa tccttcaatg tagctaaata gcgctcgatt
    11881 tgcttcttca aaatctgagt agctagtcgt gtagacttct tcttttttca atgaggcatg
    11941 gaaagattcg attccagcat tatcataagg tgttcctttt ctactataag aatgccttat
    12001 tttgttggtt ttaagccatt gttccacttc tcttgctgtg tattggctgc ccaagtcagt
    12061 gtgtagaatc attccttctg gaatgtgata atgtattttt gctttattca atgtttgaat
    12121 gacacaatcc acagtcattc gttttgaaaa tgtataacta ataattcttt tcgtgtgtaa
    12181 atccatgatt gaagataagt aacaccaacc atttttctta gtaggaatgt acgtaatgtc
    12241 agctgcccat ttctcacaga tcgtttcagt agaaaagtcc tgatttaaga gattctcttt
    12301 tgaaatgatg gaatggcaac agtttttttg acaaatttta taaggtgcag aacttctttc
    12361 cgtatgctat tccgattgtc cttgacaatg agcctcctcg gatgtgacga tcttcggcag
    12421 gagctaacag ttaaagttag actgcctcgc tagcgttagc acatccgagc tcattatcaa
    12481 ggattcgctg cgccaatctc tggttacggt aaccaaccgg tgtctcgtag ccaagtgtac
    12541 cgtgcaaccg aaggtggttc caccaattga catagtcaaa taactccaaa tccaattgtt
    12601 gtaaggtttc aaatgtgtat tgatagacaa attctacttt caacgactta taagttgatt
    12661 cagctacggc attatcaaaa ggacagcctt tatgactcaa tgatcgattg atgtcaaaag
    12721 ttgttaataa ttcatcaata gcttggttat caaactcttt tccacgatca gtatgaaaaa
    12781 tctcaacctc tgtcagaggt tgtttgataa tgctttttta ctagaacggc atctttatgt
    12841 tctccacaag aatagccgag aatttctcga ttgaacagat ccaaaatgaa acagacataa
    12901 ttccattttt tcccgactcg tacataagtc aagtctgtta cgatcgcttc taatgggttg
    12961 tctcttaaga atttacaatt tacgattcaa tacgtttgtc gttttggctt cattgcaagt
    13021 agaatgatgt actttaaaat aagcaacagt atagctcgat tttaatcctc tatttttcat
    13081 gattctacta atttttcgtc ggctgatctg aatgcctcgt tttgataagg ctttttttat
    13141 ttttcttgag ccgtaggcct ttcggctgcg gataaattct tcagcgacta cttcttcaag
    13201 ttctgattcg tctttctttg gttttgattg ataataatag gactgacgtg atagacctaa
    13261 tattctgcac atcgctgata tagggtaaag atgcttattc gcatcgatta cttgtctctt
    13321 cgtccgaata tcagcgctgc ttgctttaaa atatcatttt ccatttctaa ttgctggttt
    13381 cttttacgta gttctaacaa ttctttttgt tcaggcgtaa gattatcttt ttctttgaat
    13441 gaaccactcg ttttaaattg ctttacccat ttgtcaaatg ctgaagccgt tagttcatat
    13501 tctcgaatga tttctacacg tggctttcca gctaagtaaa gattgacgat ttgttgcttg
    13561 aattcttgtg aaaaagttct tcgtgttctc ttagacataa aaattcctcc tggtatgttt
    13621 tcttctagtc tacacacctt aatttttctg tctagttaat tgtagcctat ccatgatcgg
    13681 tttattagac ctttgaggtc tatatttttt aactacaatc gaccttaaat ttaattgttt
    13741 cattagcttt tggacatgtt taagagatac tgaaatccct tcttttaata aaacttgatg
    13801 gatctttgtg gcaccatagc gttgtttatt cgtaaaaaaa attgttgaaa tcttcttttt
    13861 caacttatta tttctgattt ctgtagctgt caacggttta ttcttataaa aataatagac
    13921 acttctaggg atttctaata accgacacaa caaagaaaca gtgtaatcct tacaccattt
    13981 ttcaataaat tgaacaagcg aatttaggtt ttccgagaga atatggctgc ggcttttttt
    14041 aagatttcaa gttcctcatt taaacgagca ttttcttttc tcagttcagc tacttctttt
    14101 ccagtcagtc ctgtggactg atcaggtaaa tataaattct tccatttgta aatcgttgct
    14161 tcagataagc catattcttt cgctaaccct cgaacagaac gtccagtttg attcagttca
    14221 acaatcattt gtttaaaatt ttcttcatat cgggtcataa aaattcatcc tctcgtagat
    14281 aagtttaatt tattcttgtc tctacgaaaa gctgtctaag atttgatact aacaccaaat
    14341 gttgtatacc cccaaacaat acacatattt tgaaggatta ggattaataa agctaaaaat
    14401 atagtaagta tactaaatat atttactata tctagtatac ttactatatt ttaatctttt
    14461 gatcgtctat tatatataat ccgttcctcg tcgtttaaag tatcaacata ggcgtccatt
    14521 gcaagtttga ctatttcata cataggaata tcttttgcat aactaagatc ccgaatggtt
    14581 tttagtacgg gaggatctac ttgaactggt tttctttctt tcgcagtgta ctttcgttcc
    14641 cctaactcga cattaatatc atctttaatt gaaatatctt cagcaccaat tttcttctta
    14701 tctttgttcg ttggtaataa tgccatttag tttaatctcc tttccaagag gtaggatatg
    14761 attggccatt gagtcagcat atttaggtgt ataatcaaat ccttcaatat caccatgttg
    14821 ttcatagtat tcaattcttt cttctaattc tgtaaagatg tctgcaaaaa tagcccacca
    14881 tcttcgatct acataatcat ttaattggat accattctca ccatacattt ctaaccgatc
    14941 cgagccttta acaatgcttt taaaaatgtt tgattcacca tacttatcaa ttgttttttg
    15001 atagtttgcg tgttgagagg aacgtcgcac tgagaaaaga acaggcaaaa ttccaatgat
    15061 ttgaagatga gactcttcaa aattcgtgac aatcggaact aaaactttat taataaaatc
    15121 ttctgttcct tccattgcgt accgttttaa ttcttgcatt gcaataatat aatctgcagc
    15181 agttaagaat gcacgaacca cattatctgt tgatggtggg cagtcgataa agatataatc
    15241 gaaatttttt ctcaattctt ctaaaggttt aatgaaggcc aatgatctct tacgacgatc
    15301 aggatatttt tcggacaagt attcattaag ttcttcagat ccaggagagc cagccataat
    15361 atataaatgt ggacttactt gagtaatacc tgaaacccag tccccttttt tgattgctgc
    15421 agacatggat cgatcaaagc tagtaatttg atacgttgat ccaatgttgg aagtagtatt
    15481 tctttgttgg tcataatcaa ttagcaacac gtctttttca aaaagttgac tagaaacaat
    15541 cgctaataaa tcattgtctg tagtcttacc aatgccgcct tttctacctg taagcattac
    15601 aacttttcct agtttatctg atgttttaaa tgcagtccgt aagttttcca tagttccctc
    15661 caatttccta tactaaatat atttagtata cttcatatac ttaatatatt cgatatatct
    15721 aatatactcc aaattgataa gaattcagac tttataagga atatagtgga tatactaaat
    15781 atatttgcta tattcagtat atcagatata ttcaaaatgt caaaatatat ttgtgcgttt
    15841 tgcgttgaca atttagagtg aaaagaggta aacttaattc tatagaaacc acagataatt
    15901 cactatttac aaagtagcaa aagcagtggt actctttaaa cagaaaaatt acataaaaaa
    15961 aagagacgga aataattgat tttagcagaa tcaattattt ctgaatcaca tggttaggac
    16021 atgtgttcac gctcgtctca agaatatgta agtagctacc aactacttat attaaaagtt
    16081 gatcggatac cttttccgat tgcctttatt ctaccaaaaa aaggcgagca actcaacaga
    16141 agaacgttta tttagtgtac ttaaattaaa tattcttaaa cactgaagag agaacacata
    16201 ctatggctga gattaaagct aaactgtgtt ctctttttgt ttgagaaagg acgttacaat
    16261 cgatgttgag cgaaatttat tcagctattc ttaaaaacgg cttacgtaca tacaaatatc
    16321 gaaacagcac catgaaacct gcaggtcata ttgattccag taggagtggt gctatctttg
    16381 cgtaccgtag caagcaatta atgaatgttg ggcgtggtat ggtcattact tcggaagaag
    16441 ctattttaga gaacgaaaag aaactaacgc attggacacc taatacctat cgctttggta
    16501 cttatgcgga tgattatcga acagtggtta aaggtcattc tgagaaaaac ttaagtcaaa
    16561 tcaacacatt tgtagttgat atcgattcaa aagagaacca tcaaggcgaa attattttag
    16621 cctgtttaga tcaagtgggc tacatgccaa cgctaatttt agaatcagat catggttatc
    16681 aagtttattt tgtactaaag acaccaggct atgtcacaaa gaaaagcaat ttcaaggtga
    16741 tcgatgtagc aaaaaaaatt tcaaaaacaa ttcgtctaca gttggctgaa aaattgtccg
    16801 gagtggatct aggatgtaac cacttcggaa ttgctcgatt cccacaagaa aataatattg
    16861 tttttaaaga aatgaactat cagtattctt ttgctgactg gattcaatgg tcgatgaagc
    16921 ttgaatcaga tcaaaaaaca aaagaagatc gagaacgaaa actaattgtc tttcctgaga
    16981 aaaaagaaat tcgtcaagta gatgaaccgt ggttcgatat gttacttcat aaagcaaaca
    17041 tcgtcggcgg agaaggacgt cttggtcgaa ataatgtgat tttcacgctt tctttagctt
    17101 attactcttc aggttatgca ttggatactt gcgaatacaa tatgttcaca ttcaacgaac
    17161 ggttacagga accgctatct gaagcagaat tagtaaagat tgtacgtagt gcttattctg
    17221 gtaattatca agcagcaagc agagaattca ttattgaatt gtgccagcaa tgggtttcac
    17281 caaccttaaa aaataatgat ctgttcattc aacgacgtgg ctggtggaaa tttaaaaagc
    17341 cacgagatca aagaaaatat agtcataaaa atgaatggaa agaagatcta ttggcttatc
    17401 taagcgaaaa aagtcttgct agtgagcctt atttgaacct gtcaaaaaaa gaattggctt
    17461 cacaactaac gatgcccaag cgaacacttg ataagatctt aaatgagtta cgagaggaga
    17521 ataaagtctt ctacaaggta acacgaggaa gaaatggcgg aatgatcatt gcttcagtca
    17581 aaacgttgtt cgcgagggtt attcaactaa gtaaagaaaa gagactagat tattcaacaa
    17641 ggatcacaga ggtatttgga ctcaacgaga gcttagtaga agagacattt agacaattat
    17701 caaaccccct tgaaccctct taaagaagtg aatctttttg aagtagatac aggttgaaaa
    17761 ataaaaggtg caaattgcct tatacaatat atctatgata cttctgtagg tgttgctccc
    17821 tttgcctagc tacgtgtagg tggtatactt ataactgcgt atgcagtctt atgtatcatg
    17881 gaggaagttg tatgtcgtta aaaaattatg tctattcaca tcctgttaat gtgtttatca
    17941 ttggtcgttt gggattatct gtggaagtgt tctgtgagct ttatggtttt aagcagggga
    18001 ctttgtcttc ttgggttacc agggaaaaaa cagttgcttc tttgccgatt gagtttcttc
    18061 atgggttgtc cttagcttct ggggagacta tggatcaggt atatgattgt ttgtgtgtgt
    18121 tagagcagga gtatattgag tataaaatag ctaacgagtt aagaaaaagg aagaagtata
    18181 tccagtagta gtttttattt actcttaatt gtcaaattaa gttagacaaa tcatcaatac
    18241 ttacgtaact caaaaatact tccaaaggtg ttcgataatt cagtgatttt ctaggaatat
    18301 tatttcgttt cgatgcgaca gattgaataa aagattcatc tactgaattg aaatccatag
    18361 atttcaataa accatctcta cgtaacaatc cgttagaatt ctcgtttagg cctctttgag
    18421 acggcgttcc tggatcagcg aaataaatgg caatatcatt ggcattactg atctgtttcc
    18481 aatttgaaaa ttcctttcca caatcaaaag tgatggattt gaatagattt ttcggtacag
    18541 attcaaacca atgatttaat ttgttttcaa tatcaatcgc ttgtctacca cacggtttca
    18601 atgtgatgat aacttttgat aatcgctcaa ctaaggtaat tacagcactt ttatgtttca
    18661 aacctacgat agtgtcacct tcaaggtgac caaactcatt tgagaattgg ctataatctt
    18721 tttcacgttc atgaatagag cgacggaaag cttgttttcc acgtctttct tgatgtccat
    18781 ttggtttacg cttgcctttc atcggtaagt cagaaggatt aaatagcccc tttttgaaca
    18841 tacgataaat ggtacgagca gaacaacgaa taggaaacgc tgcacgacca acaatcacat
    18901 cgggtgtcca tccttgaaca acctttcttt gaatatattc tgattgttcc tcaggtaaaa
    18961 caagcgggcg tctaccacag tttgatttgt ttttcttata ctgttgatag tactctaagg
    19021 ctgattttcc ttgcttgaag aacaggtata ctttatggat cgtttgtctt gaacgattca
    19081 agaaatgcgc agttttcgca acagattgat ttattttgaa ataagactct atcattacaa
    19141 gttcatccgt tgtaagatgg gtataggtca tttgtactca ctccttataa ttctttggac
    19201 ggaactattt tgagtgtagc acaaatgact tttttagttg tctagcttaa ttttacaatc
    19261 ggcgtactta aaaaattggt tcattgagca acctatcgct gctaaagtag caaaggaaaa
    19321 cgttaaagga aagaatttag ggaagaaatc gaaatgaaat cggacgactg gattgaacac
    19381 acctggcttc ctctgaaaag aaatagcaaa cgaaagaaat tttgggtatt gcaattatgg
    19441 ccattaggaa aactttgtag ttacggggca cgacttattc ttgatttaac cgtttactcg
    19501 attggagtat ttggaacggc ttattacttg ataaatgatc ccaataggat tgcagcatca
    19561 gacattttta cagtgctaat gacgttgatt tttgtcatgc tattgtctat ttttactcaa
    19621 ttagtcatgc aatttaccag gcaaaaatta acagcaggaa aacttttcaa aagcctattc
    19681 gatttaattg ttgtaaatgc aattctagcg actttaataa aacaaattga acaatctgga
    19741 agaacaacaa aaggagtgaa ggatttttta ttgacgttta ttcactttgt aaatggacac
    19801 atgtatttgt ttgaagtggg tattattctc ttttttgtcg ggtatgtatc cacgaaactt
    19861 attaaacgtt caattgaaaa agaaattgaa attgacctga ttccgacact tcttggttat
    19921 gagtacaatg ttgattttgg aagatatgac gaattgatca atacggatat cgttcacacg
    19981 agtatccatg aatctactgt taagggcatc agatctattt taaaaaatgg ccaggaaatg
    20041 aaagcaatag atcgaagaaa agagatctac aatttttatg aagtcgaagc aagcctacga
    20101 tatggcatcg gaaaattgtg gttagatgga agtaatattc gtcaaaacaa accatacatt
    20161 ttttggaaca gtagtgattt agaacttcca atgaaagata aataattatg gagaatcgtt
    20221 tgaaaatgat tcctctatac aggattgtgc ttatagttgg catggagtta aaaaccttaa
    20281 agaaggagca gttattcaaa gtgaaagaac gtcataatca gttactatgt agtaaaacgg
    20341 gtacgatgaa cattgaatac cgaacagaat atttagaatt aacgaatcaa actgattttt
    20401 tgttgattcg aaagcaacaa gagcaagaaa aaaattaatg gctacttata gccaattgat
    20461 tgatgaatgg gaacaattca aggaatctaa aattccagaa cgtctctaaa agcctttatt
    20521 tgtaaagagc ataaggaaac gtgggaacac tttttaaaaa aagtgttcca ataggctgtg
    20581 cctattacgt ttctcaaaaa taaatgtggg aacaaaatat ttaaaaatag gatagtgaaa
    20641 ggatagtgaa ggatagtaaa aggagggtag taggatagca aaaaggatag gctccttttc
    20701 gctatccttt ttaaattgga ggaattaata tggaagtaag aataagaaat gtggatgaga
    20761 aaatcgtaag agaaattgat cgaagatgtg acgaacggtt ctgttgcaaa gttttaaatc
    20821 tactatcaaa taaggtagaa taatagaaaa agatggcagg aggaatgacg atgaatcatt
    20881 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
    20941 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
    21001 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
    21061 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
    21121 gaaaatggca ttatttgtat cgagccatcg attggttacg taaaaaacgg gacacacaag
    21181 cagcctatgc ttttcttaag cggttagtga agcagtttga tgaaccgaag gttgtagtca
    21241 cagataaagc ccctctatta caagtgcctt taagaaacta aaagaatagt gcctttaaga
    21301 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
    21361 atttgattga acaagactat cgtccagtaa agagacgcaa taaattctat cgaagtttac
    21421 gcactgcctc taccacgatt aaaggcatgg aagccattcg aggattatat aagaaaaccc
    21481 gaaaagaagg cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa
    21541 tcccagctta aatcatagat accgtaaggg attttattct ttatttaaaa ctttgcaaca
    21601 gaacccatca aggtttaatt ttttatgtat ttcttttaac aaaccaccat aggagattaa
    21661 ccttttacgg tgtaaacctt cctccaaatc agacaaacgt ttcaaattct tttcttcatc
    21721 atcggtcata aaatccgtat cctttacagg atattttgca gtttcgtcaa ttgccgattg
    21781 tatatccgat ttatatttat ttttcggtcg aatcatttga acttttacat ttggatcata
    21841 gtctaatttc attgcctttt tccaaaattg aatccattgt ttttgattca cgtagttttc
    21901 tgtattctta aaataagttg gttccacaca taccaataca tgcatgtgct gattataaga
    21961 attatcttta ttatttattg tcacttccgt tgcacgcata aaaccaacaa gatttttatt
    22021 aattttttta tattgcatca ttcggcgaaa tccttgagcc atatctgaca aactcttatt
    22081 taattcttcg ccatcataaa catttttaac tgttaatgtg agaaacaacc aacgaactgt
    22141 tggcttttgt ttaataactt cagcaacaac cttttgtgac tgaatgccat gtttcattgc
    22201 tctcctccag ttgcacattg gacaaagcct ggatttacaa aaccacactc gatacaactt
    22261 tctttcgcct gtttcacgat tttgtttata ctctaatatt tcagcacaat cttttactct
    22321 ttcagccttt ttaaattcaa gaatatgcag aagttcaaag taatcaacat tagcgatttt
    22381 cttttctctc catggtctca cttttccact ttttgtcttg tccactaaaa cccttgattt
    22441 ttcatctgaa taaatgctac tattaggaca cataatatta aaagaaaccc ccatctattt
    22501 agttatttgt ttggtcactt ataactttaa cagatggggt ttttctgtgc aaccaatttt
    22561 aagggttttc aatactttaa aacacataca taccaacact tcaacgcacc tttcagcaac
    22621 taaaataaaa atgacgttat ttctatatgt atcaagataa gaaagaacaa gttcaaaacc
    22681 atcaaaaaaa gacacctttt caggtgcttt ttttatttta taaactcatt ccctgatctc
    22741 gacttcgttc tttttttacc tctcggttat gagttagttc aaattcgttc tttttaggtt
    22801 ctaaatcgtg tttttcttgg aattgtgctg ttttatcctt taccttgtct acaaacccct
    22861 taaaaacgtt tttaaaggct tttaagccgt ctgtacgttc cttaaggaat tccttagtgc
    22921 tttcatagat taaactcaca tcacgcttta aatcgcttat tttagacttt aaagacttgt
    22981 tttcttcaag caactcatta taatcattta cattttcatt aaatcgctct acaagaccac
    23041 tatatttttc tttaacttgc ccatgttctt tacttaattt tttatattct ctcgccatat
    23101 cagtactcat gagatttcta acatgctgtt ttaacctatc gttatctctc gcagcagtca
    23161 ctaagttttt ataatcacgc tccgatataa caacattttt ggttggtttc ttttctgttt
    23221 tcattatttc ttttcccaaa ccaaacatgg acttttcacc cgttggcact tcaacacttt
    23281 tcatgtgtcg tttcgctggt acttctaaat ctgatttaac tttatcgcta taagcagtcc
    23341 attcatcttt tttaactgct aaattttttt ctagaaaatc aatctctttt tccaaagttt
    23401 gttttttaaa tttagctgtc tcaatatgtt tacggtcaga gccacgttca ccacgcttca
    23461 actcaaaacc ctgttttttc atatgctcgg ggaatttatc ttgtagccat aacagttctt
    23521 gacgattaaa cacatttttt ccttgcagtt ttccatcacg cataggcaca acacctaaat
    23581 gcatgtgagg ggtttgctca tcattatgaa ctgttgcata agcaatattt tgcttgccat
    23641 atcgttcgga aaataattta taactttcct caaaaaatcg tttttgttct cctggatcca
    23701 gttgctcaaa aaaatctcgg tcagatgtta ctagcaactc atttacaaga acagcatctt
    23761 tcctcgtttt tcttgtacct gttttttgtg attcaataat ttctttgaca cgttcgttgt
    23821 aatcaatatt tttatcattt ttcaaatcat aattttcacg tgttcgctca tggtcaatat
    23881 catcattcgt tctacttttt cgctctcttt gattatgaaa ttgcatgcct tttagtccag
    23941 ctgatttcac tttttgcatt ctacaaactg cataactcat atgtaaatcg ctccttttta
    24001 ggtggcacaa atgtgaggca ttttcgctct ttccggcaac cacttccaag taaagtataa
    24061 cacactatac tttatattca taaagtgtgt gctctgcgag gctgtcggca gtgccgacca
    24121 aaaccataaa acctttaaga cctttctttt ttttacgaga aaaaagaaac aaaaaaacct
    24181 gccctctgcc acctcagcaa aggggggttt tgctctcgtg ctcgtttaaa aatcagcaag
    24241 ggacaggtag tattttttga gaagatcact caaaaaatct ccacctttaa acccttgcca
    24301 atttttattt tgtccgtttt gtctagctta ccgaaagcca gactcagcaa gaataaaatt
    24361 tttattgtct ttcggttttc tagtgtaacg gacaaaacca ctcaaaataa aaaagataca
    24421 agagaggtct ctcgtatctt ttattcagca atcgcgccct ttaatggaac aaaaagattt
    24481 gaattcctgt tataaaaaaa ggatcaattt tgaactctct cccaaagttg atcccttaac
    24541 gatttagaaa tccctttgag aatgtttata tacattcaag gtaaccagcc aactaatgac
    24601 aatgattcct gaaaaaagta ataacaaatt actatacaga taagttgact gatcaacttc
    24661 cataggtaac aacctttgat caagtaaggg tatggataat aaaccaccta caattgcaat
    24721 acctgttccc tctgataaaa agctggtaaa gttaagcaaa ctcattccag caccagcttc
    24781 ctgctgtttc aagctacttg aaacaattgt tgatataact gttttggtga acgaaagccc
    24841 acctaaaaca aatacgatta taattgtcat gaaccatgat gttgtttcta aaagaaagga
    24901 agcagttaaa aagctaacag aaagaaatgt aactccgatg tttaacacgt ataaaggacc
    24961 tcttctatca acaagtatcc caccaatgta gccgaaaata atgacactca ttgttccagg
    25021 gaaaataatt acacttccga tttcggcagt acttagctgg tgaacatctt tcatcatata
    25081 aggaaccata gagacaaacc ctgctactgt tccaaatata attcccccac aaagaactcc
    25141 aatcataaaa ggtatatttt tccctaatcc gggatcaaca aaaggatctg ttactttcct
    25201 gatatgtttt acaaatatca ggaatgacag cacgctaacg ataagaaaag aaatgctata
    25261 tgatgttgta aacaacataa aaaatacaat gcctacagac attagtataa ttcctttgat
    25321 atcaaaatga ccttttatcc ttacttcttt ctttaataat ttcataagaa acggaacagt
    25381 gataattgtt atcataggaa tgagtagaag ataggaccaa tgaatataat gggctatcat
    25441 tccaccaatc gctggaccga ctccttctcc catggctact atcgatccaa taagaccaaa
    25501 tgctttaccc ctattttcct ttggaatata gcgcgcaact acaaccatta cgagtgctgg
    25561 aaatgcagct gcaccagccc cttgaataaa acgagccata ataagtaagg aaaagaaaga
    25621 atggccaaca aacccaatta ccgacccgaa acaatttatt ataattccaa ataggagtaa
    25681 ccttttgatg cctaattgat cagatagctt tccatataca gctgttccaa tggaaaaggt
    25741 taacataaag gctgtgttca cccagtttgt actcgcaggt ggtttattaa aatcatttgc
    25801 aatatcaggt aatgagacgt tcaaaaccat ttcatttaat acgctaaaaa aagataaaat
    25861 gcaaagccaa attaaaattt ggttgtgtcg taaattcgat tgtgaatagg atgtattcac
    25921 atttcaccct ccaataatga gggcagacgt agtttatagg gttaatgata cgcttccctc
    25981 ttttaattga accctgttac attcatttca cttcataatt aattcctcct ttacacttta
    26041 attcaaatct ttattaaaaa atatttcatc ttatttaaca agaaaccata tttatataac
    26101 aacataaaat acactaagtt attttattga acatatatcg tactttatct atccgactat
    26161 ttggacgacg gggctggcaa acaggttcac cggtagtaac atggtaccct tttaactctg
    26221 ttaaacaaac actacgtcca tttgtaaaga aagttaaatc actacgatat tcttgaatac
    26281 accgagcagg gatttctcca ctaagaatga cctcattatt tttcaattga gtgtctacga
    26341 tgttcgcaca atatttagga gcatcgttgt atgctcgtga aagatattcc tgtggcgcat
    26401 aaattttaaa actaagatat ggctctaaca attctgttcc agcttttttt aagacttgtt
    26461 ccaatacaat aggagcaagc atccgaaaat ctgctggggt actaacaggg ctatagtata
    26521 agccatactt aaaacagatg gttctgttgc aaagttttaa atctactatc aaataaggta
    26581 gaataataga aaaagatagc aggaggaatg acgatgaatc attttaaagg aaagcaattt
    26641 cagcaggatg tgattattgt agccgtgggc tactatcttc gttataacct tagctatcgt
    26701 gaagttcaag aaatcttata tgatcgtggc attaacgttt ctcatacgac gatttatcgt
    26761 tgggtgcaag aatatggcaa actactctat caaatttgga aaaagaaaaa taaaaaatcc
    26821 ttttattcat ggaaaatgga tgaaacgtac atcaaaatta aaggaaaatg gcattatttg
    26881 tatcgagcca tcgatgcaga tggtttaacc ttggatattt ggttacgtaa aaaacgggac
    26941 acacaagcag cctatgcttt tcttaagcgg ttagtgaagc agtttgatga accgaaggtt
    27001 gtagtcacag ataaagcccc ctctattaca agtgccttta agaaactaaa agaatacggc
    27061 ttttatcaag ggacagaaca tcgtaccatt aaatacctga ataatttgat tgaacaagac
    27121 catcgtccag taaagagacg caataaattc tatcgaagtt tacgcactgc ctctaccacg
    27181 attaaaggca tggaagccat tcgaggatta tataagaaaa cccgaaaaga aggcactctc
    27241 ttcgggtttt cggtctgtac tgaaatcaag gtattattgg gaatcccagc ttaaatcata
    27301 gataccgtaa gggattttat tctttattta aaactttgca acagaaccta tgatcggttt
    27361 tgttccttat ttagttgata cggacagcgc aacgataaaa tcaaacctgg aagaactgta
    27421 taagcaacat aaagaagata acttggtttt ccaaaatatt atcaagcgaa gtaataaagt
    27481 aagtacctgg tctaaaaacg gcattacaga gcataaaggc tatgacaaaa aagttttatc
    27541 catgtataag aacgtatttt ttgaaatgct tgagcgaatt attcaattag aaaacgaaaa
    27601 agaatagaat cacaaatcac aagtgattaa tcacaaatca cttgtgattt gtgattgttg
    27661 atgataaaat aagagtaaga agaaatagaa agaagtgagt gattgtggga aatttaggcg
    27721 cacaaaaagc aaaacgaaat gatacaccaa tcagtgcaaa aaaagatata atgggagata
    27781 agacggttcg tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg
    27841 gcggaaacgt aaaagaagtt atggatcaag ccttagaaga atatatacgg aaatatttac
    27901 ctgacaaact ttaaaaggag tgaaaatgaa atggcagtta cgtatgaaaa aacatttgaa
    27961 atagagatca ttaacgaatt atcggcaagc gtttataatc gagtattaaa ctatgttttg
    28021 aaccatgaat taaataaaaa tgactctcaa ttattggaag tcaatttatt aaaccaatta
    28081 aagcttgcaa aacgtgtaaa tctttttgat tattctttag aagaattaca agccgttcat
    28141 gagtattggc ggtcaatgaa tcgttactca aaacaagttt tgaataaaga gaaagtggct
    28201 taatatggca aatatagtca attttactga caaacaattt gagaatcgct taaatgataa
    28261 tttagaagaa ttgattcaag gaaaaaaagc ggttgaatcg ccaaccgctt ttttacttgg
    28321 tgggcaacca gggtcaggga aaaccagttt gcgatcagca atttttgaag aaacacaagg
    28381 gaatgttatt gtcattgata atgatacctt taaacaacag caccctaatt ttgatgaact
    28441 agtgaaactt tatgaaaaag acgtagtaaa acacgttacc ccttattcta atcgcatgac
    28501 agaagcgatc ataagccgtt tgagcgatca agggtataat ttggtgatcg aaggtacagg
    28561 acgaacaaca gacgttccta ttcaaaccgc aacaatgctt caagccaaag gttatgaaac
    28621 aaaaatgtat gtcatggcag tacctaaaat caactcatat ttaggaacaa ttgaacgata
    28681 tgaaaccatg tatgcagatg atccaatgac agccagggca acaccaaaac aagcgcatga
    28741 tattgttgtc aaaaacttac cgaccaattt agaaaccctt cataaaacgg gcttat
//
