LOCUS       AP026616                2057 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA94 plasmid pAA94_3 DNA, complete sequence.
ACCESSION   AP026616
VERSION     AP026616.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466809
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA94"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA94_3"
                     /geo_loc_name="Japan"
                     /collection_date="2011"
     gene            194..796
                     /locus_tag="EfmAA94_33860"
     CDS             194..796
                     /locus_tag="EfmAA94_33860"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002919940.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein"
                     /protein_id="BDP72213.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLHD
                     KDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHMDN
                     PEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYAMSE
                     RFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
                     /name="plasmid replication protein"
     gene            complement(932..1189)
                     /locus_tag="EfmAA94_33870"
     CDS             complement(932..1189)
                     /locus_tag="EfmAA94_33870"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP72214.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTNI
                     LELNSEEQKEHKRKLIAPSYRKAAKSAVPTGFLTKQPYIF"
                     /name="hypothetical protein"
     gene            complement(1190..1411)
                     /locus_tag="EfmAA94_33880"
     CDS             complement(1190..1411)
                     /locus_tag="EfmAA94_33880"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP72215.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKEY
                     RSAGRRNYLFSPEALKKLEKVITTKEGE"
                     /name="hypothetical protein"
     Typing          complement(855..1030)
                     /name="MOBV"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.435"
                     /coverage="100.0"
                     /evalue="9.330000000000001e-86"
     Typing          195..724
                     /name="rep_cluster_2351"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.812"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 taacgcttac taaggaagtg gcagataatc ttgataaaat tgcgaaacaa atgggtctta
       61 gtaagtctgg tcttattaca atttgggtta atgctaacgg aaaggaaaat gagcaaaaaa
      121 aaaatagcaa aagcattagt gttggcgcac tgcttttgct gttagactgt tgttcaatta
      181 aggtgattat agcatgacga gtagaaaaac taaagatgaa cgcacaagga attggacgtt
      241 tgtagtctat cctgaatcgg ctcctgaaaa ttggagggag tttttagatg agttacatgt
      301 tccttgggtt gagagtccac ttcatgataa agatgtgaat cctgacggtg aattgaaaaa
      361 gcctcattgg catgttttga taatgtttag cggtaaaaag acgtatgatc aaatacgtga
      421 gataactcaa aagattcggt ctccaaaccc gcaaaaatgt gctaatgcaa aaggtatggt
      481 gcgatatttt gctcatatgg ataatccaga aaaatttcaa tatgctaaaa gtgatattat
      541 cgctcatggt ggtgcagaaa ttgctagtta tttgtcagta acgagtgcag aacgatatga
      601 gttgattaga gagatgatgt cgtttgttga ttctaagaat atcactgaaa tcaaagattt
      661 aattgactat gcaatgtcag aaagatttga tgattggttt ccgcttcttt gtgataattc
      721 agcttatatt atcgggcaat atataaaatc gaatcgtcat ggtggaagtg taaattcaaa
      781 aataaataaa ggataagagc tgttaatttc agctcttttt ttgtgcgtga aagtcttagt
      841 cactatgacc aaaactacgg acgaaactat gaccaaaagt aggtactata ttatgaccga
      901 aaataggtcc aaaatggatt tttttgtgat atcaaaaaat ataaggttgt tttgtaagaa
      961 aaccagttgg cacagccgac ttagccgcct tcctgtagga aggtgctatc agttttctct
     1021 tatgctcttt ttgttcttca gagtttaatt ccagaatatt tgtgagaagt aggtcaaaga
     1081 ggtcggtggt ttcttcgact tttccgccgg tcaattcgat aaaatcattg gaatatgatt
     1141 ctagcaattt ttctagttgt tcaacgttga tagttgtttc tttaggcatt tactcacctt
     1201 ctttggtggt tatgactttt tctagttttt taagggcttc tgggctgaaa agatagtttc
     1261 ttctaccggc agagcgatat tcttttcctt cagtcagatg ttctttcgct aagcgcaaaa
     1321 cgtaagcttg gttgtaatca agttcttgtg caagttcgct ggtcgtcata atttcggcca
     1381 aagtgaattt ttctgatttt atcactctca tttatgctcc ttccatttaa cgtccgtcta
     1441 tattacgtga gtacgtcgga cttacgacat aagcttataa tataagtttt ctgtgttgtg
     1501 gtcaatttta tattatcgaa atgactagta gaaaaaatca atgctctaaa aagggtctat
     1561 tttgcgtttt aagcgattta gcgtctattt cgtttagtta tcggcgcaat cgttaaaaca
     1621 cgtaagagaa gcgagaatgg agcctgtaag gcgactggta gcgaaacggt cgtgttagat
     1681 tgcaaaagcc gatagctgaa cgaaatagta agcgtggcgc ctgcatatat ttcggtagaa
     1741 ggaggtcggg gttcggggcg gagccctgag gtttgttttt tggttttggt gtttatgaat
     1801 tgggcggagg agggggggga ctacgacacc cccccttatt ggtcattggt cattggtcga
     1861 tagctgttta gccttactct cccaagggtt tcagcgtttt tttttgacaa aatcagtaat
     1921 gccaagggtt taaggcctat tattcgctaa ttagtatcta aactgttgca ccttagttac
     1981 ttttttgtta ctatggtttt taagaaaaag tcgttggagg atacgaatta tgatcagtaa
     2041 tgaaaaaaag agagtcg
//
