LOCUS       AP026611                6176 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA55 plasmid pAA55_6 DNA, complete sequence.
ACCESSION   AP026611
VERSION     AP026611.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466808
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6176)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA55"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA55_6"
                     /geo_loc_name="Japan"
                     /collection_date="2010"
     gene            390..869
                     /locus_tag="EfmAA55_32490"
     CDS             390..869
                     /locus_tag="EfmAA55_32490"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68820.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIK"
                     /name="hypothetical protein"
     gene            1096..1542
                     /locus_tag="EfmAA55_32500"
     CDS             1096..1542
                     /locus_tag="EfmAA55_32500"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68821.1"
                     /translation="MSWTDENEPTINNEPTSYEELELAFAKLQKKYEENNELLRLNNRK
                     LDRLEETMTNTLKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDSQVKK
                     FHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
                     /name="hypothetical protein"
     gene            1549..2238
                     /locus_tag="EfmAA55_32510"
     CDS             1549..2238
                     /locus_tag="EfmAA55_32510"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68822.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLPG
                     VIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTTCY
                     EEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQTEEA
                     CYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            3082..4026
                     /gene="rep"
                     /locus_tag="EfmAA55_32520"
     CDS             3082..4026
                     /gene="rep"
                     /locus_tag="EfmAA55_32520"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP68823.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep"
     gene            4047..4580
                     /locus_tag="EfmAA55_32530"
     CDS             4047..4580
                     /locus_tag="EfmAA55_32530"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDP68824.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLREQL
                     ALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATETMD
                     EQTKNWKKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            4595..4765
                     /locus_tag="EfmAA55_32540"
     CDS             4595..4765
                     /locus_tag="EfmAA55_32540"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68825.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNSQ
                     NEEVFVEKLDK"
                     /name="hypothetical protein"
     gene            5219..5443
                     /locus_tag="EfmAA55_32550"
     CDS             5219..5443
                     /locus_tag="EfmAA55_32550"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68826.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEKC
                     WVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
                     /name="hypothetical protein"
     gene            5463..5750
                     /locus_tag="EfmAA55_32560"
     CDS             5463..5750
                     /locus_tag="EfmAA55_32560"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68827.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKSV
                     GEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
                     /name="hypothetical protein"
     BGC             219..6176
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          3083..4026
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          391..1301
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.763"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gtaggacgta ctgaaagggt gggacttatg agcgaacaaa atcaatattt ggctagcaat
       61 cattctaaaa aatataccta tcgatccgag ccaaaacaaa tcagttttcg agtgagcgaa
      121 tccgaatttg caaagctgaa gcagtcagct gaagctttgc aaatgagtgt gccggctttc
      181 gtgaaagcca aggcacaaaa cgcgcgtctg gtgacaccga aagtggcgcc agacattgct
      241 caggcaatcg cgcgcgattt agccaaagct ggcgggaata tcaatcaaat tgccaagtgg
      301 tgcaacacac accaacacga cgttgatcct ggcgacgccc aacgcttatc cgaaaaccta
      361 aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagc cgaaccactt
      421 cttgtagccg agcgatcaat tacgccgaac cacgagcaac cgtcaaaaca gggataaact
      481 gtgacattga ttatgccaaa agcgaaatga aacaaattcg catgctgtat ggaaaagacg
      541 atcgtgtaca agcacactta ttgatccagt cttttcgacc aggagaaatt accgctgaaa
      601 aagccaatca actaggggca gagtatgcga aaaaaatagc ccctgaccac caaatcgcga
      661 tttacacaca tacagacaaa gaccatattc ataaccacat tgtcattaac tccgtcaatc
      721 tggaaactgg gaacaaattt caagcccatg gtcaagcatt tttaaacaaa tgttatgacc
      781 taaatgacga gatttgtctt gaacatgact taacaatcac tgaacgaggg aagaaacccg
      841 aaaaacgaac catgtctgaa atcaaataaa aaagaaaaaa tgaacctgtt tggaaagacg
      901 aaattcggtt tgcgattgat caaacaatga aaaacaaaaa aactcgaacg tataaccaat
      961 tttgtgattc tcttaaaaca ttcggcattc attgcttgaa tcgtgggaaa aattttactt
     1021 acgaattgat ccacaaaaag aaaaaagtac gatccaacaa actaggaaag gattacgaaa
     1081 aggagacgat tttacatgag ctggacagac gagaacgagc ctacaatcaa caacgaacca
     1141 acgagctacg aagagctcga gctagcattc gcgaaactgc aaaagaagta cgaagagaac
     1201 aacgaactct tacgactgaa caaccggaaa ttagaccgac tcgaagaaac catgaccaac
     1261 acgttgaaag aggagcagac taccaacaag aattatcgtt ctaaaacgtt acaagacata
     1321 gaaaatgaaa taaagaaaca acgtgctttt cttgcacctt ataccaaaga aagaatacaa
     1381 actacaattg attcacaagt caaaaaattc caccatgcgc tattaaacca gcgaaataaa
     1441 gaacagaaaa aagtaattat gcagcgatca atggatattc ttgccccctt atgttgctta
     1501 atcatgacac tgttagcctt ttggctggta tggggagaat aaatagaaat ggcattctat
     1561 ttaccctatc tactcatttt tgccagtatt ttaggaatta tttggcttag ctataaggct
     1621 tatcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagaggtt tttattgcca
     1681 ggggttatat ttacattatc aatcgtttta agtggtttat tcggcgtttt agagggaaat
     1741 aaacgtattt caagaagtat tttattaggg gatatcactc aaaagtatga agcagcatgt
     1801 gataaaaaga aaaaggagca agtgttaaca caaaaaatgg aagagtttac cacttgttat
     1861 gaggaaatga acgatatttt tgtgaatcaa gaaaaacgtt tgacggataa aaacatggaa
     1921 aaactcactc gtctttatca aaacttgcca gaaaaacagc aaaaagaggt tcaagacaac
     1981 tatgagcgaa ccaaaaaaga cgtgcaatat tttaaagata cgcagacaga agaagcttgt
     2041 tataatttat tcagtgatac aatccctttt tcaactagtg aacaggaacg aaaagaacgg
     2101 caacaatcag ttacctacga acgttataaa gcactgcttc agcaagcaac gaacatacaa
     2161 aatccaacga aaaaagaaac ggcattaaat tatctaaaaa gtgtcaagga atggttagat
     2221 cagcaacagc aaaattgaat ttgatcggca atcatgttaa actaaacatg caaatgatca
     2281 aacattagat acacgaaagc cccagagagg tcggattctc tggggctttt tttcaggttg
     2341 atgtcgcctt tattgatttt acttcttgtt tcggttattc aaccaataac tgaataaaga
     2401 gacacaaact tctataagaa gggggcaaat gaacaattca aacattagat acacctcctt
     2461 ccagtcgcaa gactgtcgga taggcgacaa gttattatat cttaaaaaat taaacttgtg
     2521 tggtgttttt aagtatcgct tattttaggg ttgttagggc ttgccctgac cgcctgtaag
     2581 gcgctttaaa gctagataag gatttttatc caagactgtc ttaaaacggc ttagataagc
     2641 attcagaacc gctcaaaggc ctctttacca acatgttttt agcaaaggag cctttaggcg
     2701 actgcgcagt acaatgtgag agcgtagcga tcacaccttt ttggcgaccg taggaagcaa
     2761 ggttttggat tggcagtttt gaaaaatgcg cgaacctgtt taaagaaaaa agaagtggtt
     2821 tttaggcggg aaaattagcc cccctctttt tatatctctt tttaaacctc tttttaaacc
     2881 tctttaccgt aagctgtgtc cttactctac caatggatac agaatgttat gaaggacatt
     2941 ttgtctgttt atgaaggaca ttttgtctgt ttatgaagga cattttgtct gtttatgaag
     3001 gacattttgt ctgtttgaac gtcaaatatg aatgtcttga catttaaaaa atgtcattca
     3061 taattggatt gaggtgatta tatggctaat gagattgtaa aataccacca tgaattaaat
     3121 acaattcctt taagaaaatt tacaccggtt gaaatgaatt tgtttttttc catcgtgtct
     3181 agaatgcgtg aaaaagggga taagacagtt cgttttacgt ttgaccaact aaaagattta
     3241 agtaactaca aagcaactgc aaatgtacga tttgtagaag atttaaaagc aacttatgaa
     3301 aaaatcttat ctttacgctt tggtcgtaga agtgagtctg ggctttctta cgatatgttt
     3361 gtaatgttta cagaatttaa aatcaacggg gacgtagaaa atccttacgt agatatacga
     3421 atttacgaaa aagcactgcc tttgttaaat aatttagacg agtgggtacg ctattcttta
     3481 caacaattca acgagctaca aagtggctat gcaaagacta tgtttcgtct gttaaagcaa
     3541 tacagaacca aaggtttcgc atatttctcc aaagaagact ttcttgaatt attagatatt
     3601 cctaaaagtt ataagcaacc tgatatagat aaacgagtta taaaacctat acgtcaagaa
     3661 ctaactgcaa tcttcaaagg actaacaatc aaaaagaaat acggaaaagg acggggaaaa
     3721 cctgttattg gctatcaatt tacgttcaaa cctgagatca aaaaagcaga cgactttaac
     3781 aaacatgcac caaccaaaga agaccaaaat aaagcattgg ctttagctga taaacaaaaa
     3841 gaatacgaag aacccgctac aacaattaac gaaaaaatca aacagaagaa acgagaactt
     3901 gaactcctcc aacaaatcaa acaactagaa aaagagaaaa aagctcttga aaaacaaaca
     3961 gagtccgcac tttcggaaaa agagaaaaaa agtttattat ctgatttaga taatttattc
     4021 cagtagcaac ctaggaggca accagtatgc aaccacaaga aacctttaca ataaaggaat
     4081 tgtcagatct ttttaagatg agtaggcaag caatatcgaa acatatccaa aaattagatt
     4141 cttccatgat agctaaaaat gagcgtggtt ataaggttgt attgcgttct ggagtattgc
     4201 aacttgctcg caacttagac aatcaaaggt tgctagataa gttgcataaa catgaacaaa
     4261 acaacgataa aaatgcttat ataccaaaca aaaacgtggt tgcaaacaag ttgctagatc
     4321 agttgcgtga agaaaatgaa tttttacgag aacaacttgc cctcaaagag caaaataatt
     4381 ctcatataca aaaactactc gaccagcaac aacaactcac cctgcaagcc aaccaacaaa
     4441 tcgaaaaact tcaggagcaa ctgcagttga cttatacgga agagtcttca gagaatagtt
     4501 caacgactct ttctgaagca acagaaacaa tggacgaaca aacaaagaat tggaaaaaat
     4561 ggtggcagct atggaaatga agaaggaaat acttatgaca aaaaaacact tgctattcgg
     4621 ttctatccta ctaggaatat taagctttag cccaatgtta ttatcagtgg ccaacaaagg
     4681 aaataaaaaa ggggaactaa aactcaatcc taattactac aacagtcaaa atgaggaagt
     4741 atttgtagaa aaacttgata aataagaaaa gactgctcgt acgagcagtc tttctgaaag
     4801 agattatcct attattaacc ttagaaaaga ataataaatg aatatgagat atgtaaaaag
     4861 cataacaaac acaaacaaaa ataacaagta tttaaaaaat gaccgatcaa tattcacaaa
     4921 gaattagaaa gaaacaaccg cagaaaacca agccttaaaa agagctttta acctagacca
     4981 ataaaaattc acaaaatcag cgcctggatc ccttggtaca ggcgttactt ggtagtatat
     5041 acattatgtc tactagatct taatataacg attttataat ttaatttaaa cgattatgaa
     5101 ttcaaaacta ctttttatga cgttaatcat tgttatataa taaaatgtta tataataaaa
     5161 ttactaagat tatttaatat aacaagcaat tttaaaaaaa cgaaaaggaa aaacaatcat
     5221 gaaaaagaaa gtattaaaac attgtgttat tctaggaata ttaggaactt gtctagctgg
     5281 catcggtaca ggaataaaag ttgatgcagc tacttactat ggaaatggtc tttattgtaa
     5341 caaagaaaaa tgttgggtag attggaatca agctaaagga gaaattggaa aaattattgt
     5401 taatggttgg gttaatcatg gtccatgggc acctagaagg tagtggtagg gaggttatta
     5461 taatggattt tactaaagaa gaaaaacttt taaatgcaat tagtaaagta tacaatgaag
     5521 caactataga tgactatcct gacttaaaag aaaagctctt tctttattct aaagaaatca
     5581 gtgagggaaa aagtgttggt gaagttagta tgaaattaag tagttttctt ggaagatata
     5641 ttttaaaaca taaatttgga ttacctaaat ctttaataga attacaagaa attgttagta
     5701 aggaatctca agtatataga ggatgggctt ctattggtat ttggagttaa ttctttggaa
     5761 taaaatgaaa atgggactga agaatcaatt ctgattcatc agtcccataa attatagagc
     5821 cgaaaaatta tcatatttat agatttatta atagtaaatg gcataagaat ctttgaacaa
     5881 cgaaaaaaat gtagctctac ctaattattg atttgcaaca atttcaccga aggtgaaaca
     5941 agcgaaactt tacaaaaagt ggctagcatt tgctagtctt ttttgtattg tggatattgt
     6001 ggagtggggg ggggggattc aagggggagc ttgcgcccct ttgacaattg caccaaatac
     6061 gtaggtattt ggctagcaaa tatagagctt gccaagccct ttagtgagct ttagtgagct
     6121 ttacttattg taccttttta ggtacaatgg tattgcgaat tgcgaacgat acgtcc
//
