LOCUS       AP026609               20946 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium AA55 plasmid pAA55_4 DNA, complete sequence.
ACCESSION   AP026609
VERSION     AP026609.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466808
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 20946)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..20946
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="AA55"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pAA55_4"
                     /geo_loc_name="Japan"
                     /collection_date="2010"
     gene            188..400
                     /locus_tag="EfmAA55_32100"
     CDS             188..400
                     /locus_tag="EfmAA55_32100"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68781.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAAA
                     VVITAVAKASDSILAWLKFKKEQKK"
                     /name="hypothetical protein"
     gene            562..1080
                     /locus_tag="EfmAA55_32110"
     CDS             562..1080
                     /locus_tag="EfmAA55_32110"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68782.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSEI
                     FENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEFKL
                     ALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGNLSY
                     EMISKVLSL"
                     /name="hypothetical protein"
     gene            1285..1599
                     /locus_tag="EfmAA55_32120"
     CDS             1285..1599
                     /locus_tag="EfmAA55_32120"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68783.1"
                     /translation="MEKCKATDAIFSFLRKSDEIITRENIDLENEISKLESIFSDINSL
                     KYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSIISQNMDKLRY
                     "
                     /name="hypothetical protein"
     gene            1870..2493
                     /locus_tag="EfmAA55_32130"
     CDS             1870..2493
                     /locus_tag="EfmAA55_32130"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289893.1"
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="BDP68784.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVSG
                     KNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLPSF
                     NGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYHANA
                     TGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
                     /name="resolvase"
     gene            2589..3269
                     /locus_tag="EfmAA55_32140"
     CDS             2589..3269
                     /locus_tag="EfmAA55_32140"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001015311.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDP68785.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6 family transposase"
     gene            3467..4072
                     /gene="fic"
                     /locus_tag="EfmAA55_32150"
     CDS             3467..4072
                     /gene="fic"
                     /locus_tag="EfmAA55_32150"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000599739.1"
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="BDP68786.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTFK
                     GLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEIIKK
                     YVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDLEIR
                     YLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
                     /name="fic"
     gene            4088..4660
                     /locus_tag="EfmAA55_32160"
     CDS             4088..4660
                     /locus_tag="EfmAA55_32160"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000170424.1"
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="BDP68787.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRDR
                     PKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTSMG
                     RMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELLTSG
                     KSYKEVEAITGFSRSTLFRIKKKIEES"
                     /name="resolvase"
     gene            complement(5133..5555)
                     /locus_tag="EfmAA55_32170"
     CDS             complement(5133..5555)
                     /locus_tag="EfmAA55_32170"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68788.1"
                     /translation="MDLHTKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDL
                     GSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANR
                     ALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
                     /name="hypothetical protein"
     gene            complement(5806..6069)
                     /locus_tag="EfmAA55_32180"
     CDS             complement(5806..6069)
                     /locus_tag="EfmAA55_32180"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287855.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP68789.1"
                     /translation="MSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQQLDLELFDYVN
                     WWNHLRLHGTLGYETPVGYRNQRLAQRILDNELGCANASEAV"
                     /name="transposase"
     gene            complement(6152..6382)
                     /locus_tag="EfmAA55_32190"
     CDS             complement(6152..6382)
                     /locus_tag="EfmAA55_32190"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     6371,6374"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68790.1"
                     /translation="MKQTTNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFN
                     REILGYSCGEHKDAVLVKKHLAVSNNPSDRG"
                     /name="hypothetical protein"
     gene            complement(6351..6641)
                     /locus_tag="EfmAA55_32200"
     CDS             complement(6351..6641)
                     /locus_tag="EfmAA55_32200"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     6370,6373,6503,6187,6166,6463"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68791.1"
                     /translation="MCRILGLSRQSYYYPSTKKDESELEEVVAEEFIRSRKAYGSRKIK
                     KSLIKTRHSDQPTKKISRIMKNRGLKSSYTVAYFKVHHSTCNEANDKRIES"
                     /name="hypothetical protein"
     gene            complement(6677..6967)
                     /locus_tag="EfmAA55_32210"
     CDS             complement(6677..6967)
                     /locus_tag="EfmAA55_32210"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002322074.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP68792.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKWV
                     KQFKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGRRDK"
                     /name="transposase"
     gene            complement(7008..7322)
                     /locus_tag="EfmAA55_32220"
     CDS             complement(7008..7322)
                     /locus_tag="EfmAA55_32220"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000882571.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP68793.1"
                     /translation="MLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQR
                     YGATKIHQVLLKEGISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN
                     "
                     /name="transposase"
     gene            complement(7373..7627)
                     /locus_tag="EfmAA55_32230"
     CDS             complement(7373..7627)
                     /locus_tag="EfmAA55_32230"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000199136.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BDP68794.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYLP
                     DQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
                     /name="transposase"
     gene            complement(7820..8095)
                     /gene="prgO"
                     /locus_tag="EfmAA55_32240"
     CDS             complement(7820..8095)
                     /gene="prgO"
                     /locus_tag="EfmAA55_32240"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001196543.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68795.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVDP
                     PVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
                     /name="prgO"
     gene            complement(8067..9020)
                     /gene="parA"
                     /locus_tag="EfmAA55_32250"
     CDS             complement(8067..9020)
                     /gene="parA"
                     /locus_tag="EfmAA55_32250"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000429439.1"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning ATPase"
                     /protein_id="BDP68796.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLFE
                     KDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGSPG
                     SEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAADYII
                     AMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQKTID
                     KYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYYEQHGD
                     IEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
                     /name="parA"
     gene            9632..11125
                     /gene="repR"
                     /locus_tag="EfmAA55_32260"
     CDS             9632..11125
                     /gene="repR"
                     /locus_tag="EfmAA55_32260"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000947691.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="BDP68797.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSKQ
                     LMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINTFV
                     VDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVIDVA
                     KKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSMKLES
                     DQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTLSLAYY
                     SSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELCQQWVSP
                     TLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLNLSKKELA
                     SQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLSKEKRLDYS
                     TRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
                     /name="repR"
     gene            11260..11556
                     /gene="prgN"
                     /locus_tag="EfmAA55_32270"
     CDS             11260..11556
                     /gene="prgN"
                     /locus_tag="EfmAA55_32270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000053907.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication control protein PrgN"
                     /protein_id="BDP68798.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTRE
                     KTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
                     /name="prgN"
     gene            complement(11580..12539)
                     /locus_tag="EfmAA55_32280"
     CDS             complement(11580..12539)
                     /locus_tag="EfmAA55_32280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287225.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="BDP68799.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLFF
                     KQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIRCS
                     ARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFKSITF
                     DCGKEFSNWKQISNANDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDESF
                     IQSVASKRNNIPRKSLNYRTPLEVFLSYVSIDDLSNLI"
                     /name="IS30 family transposase"
     gene            12722..13573
                     /locus_tag="EfmAA55_32290"
     CDS             12722..13573
                     /locus_tag="EfmAA55_32290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002287227.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68800.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILDL
                     TVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAGKL
                     FKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILFFVG
                     YVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKGIRSI
                     LKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDLELPMK
                     DK"
                     /name="hypothetical protein"
     gene            13594..13806
                     /locus_tag="EfmAA55_32300"
     CDS             13594..13806
                     /locus_tag="EfmAA55_32300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 13800"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68801.1"
                     /translation="MIPLYRIVLIVGMELKTLKKEQLFKVKERHNQLLCSKTGTMNIEY
                     RTEYLELTNQTDFLLIRKQQEQEKN"
                     /name="hypothetical protein"
     gene            14239..14919
                     /locus_tag="EfmAA55_32310"
     CDS             14239..14919
                     /locus_tag="EfmAA55_32310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748745.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="BDP68802.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHT
                     TIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDIWL
                     RKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLN
                     NLIEQDYRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLL
                     GIPA"
                     /name="IS6 family transposase"
     gene            15248..15424
                     /locus_tag="EfmAA55_32320"
     CDS             15248..15424
                     /locus_tag="EfmAA55_32320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68803.1"
                     /translation="MIHQSSAKKDIMGDKTVRVRADLHHIIKIETAKNGGNVKEVMDQA
                     LEEYIRKYLPDKL"
                     /name="hypothetical protein"
     gene            15441..15713
                     /locus_tag="EfmAA55_32330"
     CDS             15441..15713
                     /locus_tag="EfmAA55_32330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000301765.1"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="BDP68804.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVN
                     LLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
                     /name="antitoxin"
     gene            15715..16578
                     /locus_tag="EfmAA55_32340"
     CDS             15715..16578
                     /locus_tag="EfmAA55_32340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001284311.1"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="BDP68805.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSG
                     KTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAIIS
                     RLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYETMY
                     ADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISP
                     KETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKT
                     PKLPGI"
                     /name="toxin zeta"
     gene            complement(16841..17578)
                     /gene="ermB"
                     /locus_tag="EfmAA55_32350"
     ARG             complement(16842..17578)
                     /name="ErmB"
                     /gene="ErmB"
                     /gene_name="Erm 23S ribosomal RNA methyltransferase"
                     /tool="rgi"
                     /drug_class="macrolide antibiotic; lincosamide antibiotic;
                     streptogramin antibiotic; streptogramin A antibiotic;
                     streptogramin B antibiotic"
                     /identity="98.78"
                     /coverage="98.79"
     gene            complement(18328..19122)
                     /gene="aphA"
                     /locus_tag="EfmAA55_32360"
     CDS             complement(18328..19122)
                     /gene="aphA"
                     /locus_tag="EfmAA55_32360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_001096887.1"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-IIIa"
                     /protein_id="BDP68807.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
                     /name="aphA"
     gene            19585..20226
                     /locus_tag="EfmAA55_32370"
     CDS             19585..20226
                     /locus_tag="EfmAA55_32370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68808.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQL
                     KQRDTVIIACVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKII
                     RYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKIHM
                     IVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGFCCKVLNKE"
                     /name="hypothetical protein"
     gene            complement(20249..20569)
                     /locus_tag="EfmAA55_32380"
     CDS             complement(20249..20569)
                     /locus_tag="EfmAA55_32380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 20601"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68809.1"
                     /translation="MKQFNEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIKYLNNL
                     IEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKTRKEGTLFGFSVCTEIKVLLGI
                     PA"
                     /name="hypothetical protein"
     gene            complement(20569..20925)
                     /locus_tag="EfmAA55_32390"
     CDS             complement(20569..20925)
                     /locus_tag="EfmAA55_32390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 20601"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP68810.1"
                     /translation="MNHFKGKQFQQDVDVIIVAVGYYLRYNLSYREVQEILYDRGINVS
                     HTTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKNKTHKQPMLG"
                     /name="hypothetical protein"
     ARG             complement(18332..19122)
                     /name="aph(3')-IIIa"
                     /gene="aph(3')-IIIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             complement(16845..17578)
                     /name="erm(B)"
                     /gene="erm(B)"
                     /gene_name="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /tool="amrfinderplus"
                     /drug_class="LINCOSAMIDE/MACROLIDE/STREPTOGRAMIN"
                     /identity="99.59"
                     /coverage="100.0"
     Typing          9633..11125
                     /name="rep_cluster_185"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.866"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttctattat tctaccttat ttgatagtag atttaaaact ttgcaacaga accctaaaaa
       61 cactatatat tccgaaaagt gagtatctcc attcataaaa taatcaacat atttaaaaaa
      121 tctaattagc ttttttagtc aatctaaagt tctttattct atgatataat gttactgtag
      181 aaagtttgtg gcggtttggc aaatccaatt gaaggagcgt ggtgcctatg ggcttattag
      241 cttatcggac gtactctgta gaaaggaaat gcctgatttg ttctcattgg atcaaatcat
      301 tgctattatc ggagcagcag cagtagttat tactgccgta gcgaaagcta gcgattcgat
      361 tttagcttgg ttaaagttca aaaaagaaca aaaaaaatag agccgtctca acttttggcg
      421 aggtagacga gctctaaagc tttatttaca tgccatccgc tttgaacgga ctacactggg
      481 catgtgacag catgcccttt catttatatt atatctcatt cttttatata atgaaagtaa
      541 ataaatcatg gaggataaaa tatggataaa tttaatagat taacattaat aaatcagttt
      601 gaaattttaa aagctttaaa tcctaacgaa gaaaaatact atgctgaaaa aatagaaata
      661 ctaacagaag gttacgaata tcactatagt gaaatatttg aaaatatttc tgatccattg
      721 ccagaagaag attcgcggtt tgttctagac atattaaata tgtatagaga tattactttt
      781 agtaaaaata aattaaaaga tataaattca attgataatt attatataca atttaaaggt
      841 tttgacttta atagctctac cgaatttaag ttagcacttt atgcacaatt ttttattgaa
      901 aaattaaata ggtttcaaga aatagttgaa gatagtgatt ttaacacttt taatagccat
      961 aaaccgatga gaggtactta tataaaattt ctcgaaaatt ataatgattt aaaatctacc
     1021 aataattatc aatttggaaa cttgtcttat gagatgataa gtaaagttct aagtttgtga
     1081 tgataaatga aattaaatag tataaatgat ttatttgcta ttataggtgt tattgctaac
     1141 atactttttt cgtgtttgaa ttttagattt actcaaaaac aaaaagaaca aactcagtat
     1201 agaacaaaaa taaaaagaga ttgaaaaaca gatataaaaa atgaccataa agaaagaaga
     1261 aaaattgaag aatatacttc tttaatggaa aagtgtaaag caactgatgc aatttttagc
     1321 tttttaagaa aaagtgatga gataatcaca cgtgaaaata tagatttaga aaatgagatt
     1381 agtaaacttg aatctatctt ttccgatata aattctctta agtatttaat cgtcgaagaa
     1441 aattcggata gctttttaga atttgaaaaa tatctaacag atcttataga taatagaaat
     1501 aatgaattaa tgagacaaaa cattgcaaga ttccttacta aattagtttc tgaatggaaa
     1561 agtattattt ctcaaaatat ggataaatta agatattaag gatgttagtt ttaaaataaa
     1621 ctaatgtcct tatttttgtg ttcacatttc tgttacctaa gaaatactta aaggaggtga
     1681 tgtagccagc aacgactaca acttgaaccc gctatctaca gtttatttgt tgtttctttt
     1741 gggtatactc taatggtaca aatccatatt tgcttaaagc gttgattttt cagcggtttt
     1801 tctttattgc aaagtgtacc ctaagacctg tatactaata gtaaatagat tggataggaa
     1861 tgatggcaaa tgtatttagc ttatttaaga gtttcttccg acgatcaaag tttagctcgg
     1921 caacataagc taattgaaaa ttggtcagtc aaaaataata ttgctgaaga cgatttaaaa
     1981 attttcgagg aaaaagtctc tggaaaaaat attgaaaaca gaccagagct gcaagagctt
     2041 atttctttca tacgtgaacg agatacgatt attattccta gtcttgatcg attagcaaga
     2101 aatagtaaag atacaaaaga cttacttcaa ttgtttcgta gtaaaggtgc ttctattgaa
     2161 atattagacc taccgtcgtt taatggggta actgaccctg cattaagaga cttgctaaca
     2221 aatttagtca tagaagtgtt tagctatgtt gctgaaaacg aacggaaaaa aatcaaagag
     2281 cgtcaaaaac aaggcattga aattgcaaag aaaaatggtc gttatactgg taaaaagatt
     2341 caataccatg ccaatgcaac aggaaaaaac aaattgattt acgatgaaat tgttgatggt
     2401 ctgaagaaaa aagaaactat ttctaaaata gcaaagaacg caggagttac aagaaaaacc
     2461 gtttatcgag taaaaagaga tttaggaatg taatatgttc cagttagaaa gaagggttct
     2521 gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag atagcaggag
     2581 gaatgacgat gaatcatttt aaaggaaagc aatttcagca ggatgtgatt attgtagccg
     2641 tgggctacta tcttcgttat aaccttagct atcgtgaagt tcaagaaatc ttatatgatc
     2701 gtggcattaa cgtttctcat acgacgattt atcgttgggt gcaagaatat ggcaaactac
     2761 tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa atggatgaaa
     2821 cgtacatcaa aattaaagga aaatggcatt atttgtatcg agccatcgat gcagatggtt
     2881 taaccttgga tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta
     2941 agcggttagt gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta
     3001 ttacaagtgc ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta
     3061 ccattaaata cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata
     3121 aattctatcg aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag
     3181 gattatataa gaaaacccga aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa
     3241 tcaaggtatt attgggaatc ccagcttaaa tcatagatac cgtaagggat tttattcttt
     3301 atttaaaact ttgcaacaga accaactaga ctgtgcaaaa tagagacact tgaaaaatct
     3361 atcgggggtc agaaacttaa tagtttttga cacataaaca aaatcgcgat ataatggggt
     3421 tgagagtgtt tcataaactt atgtcataaa gttaaggaga tttcgcatga ttttagagaa
     3481 taaattggga ttaaccaatc aagttgagtt ggctaaggtt gaagaaaaac tcagtaaaca
     3541 aaaagcgaaa gaattgtacg attgtggaaa aataaatgaa atcgaagttg gtacatttaa
     3601 aggtttatct gaaattcatg aatttctatt ttcggatatt tacgattttg ccggaaaaat
     3661 ccgatcggtc aatattgcaa aaggaaattt tcgatttgca ccagttatgt atttagaaca
     3721 ttcgttacag catattgatc agatgccaca aacttctttt gaagagatta tcaagaaata
     3781 tgtggagatg aatatcgctc acccatttcg agaaggaaat ggccgaagta ctagaatttg
     3841 gctggatctc attttaaagg aagaattgca aaaagttgta gattggaact taatcgataa
     3901 agcagactat ctttctgcaa tggagcgaag tcccataaac gatttggaaa tacgctatct
     3961 aatttccaat gctctgactg ataaaattga cgatcgcgag ttatatatga aaggaatcga
     4021 tgtcagctat ttctatgaag ggtattctga gtatacgata gatgatttgt aaaagtaggt
     4081 ggaccaaatg acgaaatatg gatatgcacg tgtctcaacc gcttctcaaa gtacaaaaga
     4141 acaaattcaa cagttgcttg agaactgcgt agagaagaag aatatttttt cagaaaaatt
     4201 tacgggtaca aaacgggatc ggccaaaatt caatcagctg ttggctggtc tgaagcctgg
     4261 agatgaaata gttgttacga agcttgatcg atttgctcgt aacacgagag aagcattaga
     4321 gatcattgaa cctttactcg atgataacgt aacgatcaag gttttgaatc taggaacgat
     4381 tgaaaatacg tccatggggc gaatggtgac acggacgttg ttgagtgtag cagaaatgga
     4441 acgtgatatg attgtggaac gtacccaaga aggaaaaatg tttgcaagaa agaataatcc
     4501 taatttcaga gaaggacgtc cgaaagcaac aatcacaccg aagaagaggc acgcatatga
     4561 actgcttact tcaggtaaat cgtataagga agttgaagct ataaccggtt tcagtcgcag
     4621 tacgcttttt agaataaaga agaaaattga agaaagttaa aaaagaagtc tcagcgaacc
     4681 agacaggtaa atacttaatg cgttctagat atgagtctat tagcatgttg tgaaatataa
     4741 tattttttta tttacttgat ttctgctcaa aaaatgtgta tggtaactgt aaaaggtaaa
     4801 gtaattaaag gtatgctagc cacgcttact atattaggat accatcatat atttataatg
     4861 aacgatttgt tcgtctacca aacaacacat atcgttcagc gtaagtcacg tttaggaacg
     4921 tggctttttt agtgttatta tcgataaata atctattgac tctattgatt ttatctccat
     4981 atccctacgg tgttgattgt ctttagatct ttcatttaag aaccaaataa ctatcataaa
     5041 aatcagcact gctgctaata taataaacag cattcctatt gtgatattac tggatttgag
     5101 tcaatatctt ggacacattt tagtagagac ggttaagcca ttttttcttt tgctaattct
     5161 tcaaattcct gtggggttag atagtgaatc gagctatgga ttcgatttcg gttataaaat
     5221 ccttcaatgt agctaaatag cgctcgattt gcttcttcaa aatctgagta gctagtcgtg
     5281 tagacttctt cttttttcaa tgaggcatgg aaagattcga ttccagcatt atcataaggt
     5341 gttccttttc tactataaga atgccttatt ttgttggttt taagccattg ttccacttct
     5401 cttgctgtgt attggctgcc caagtcagtg tgtagaatca ttccttctgg aatgtgataa
     5461 tgtatttttg ctttattcaa tgtttgaatg acacaatcca cagtcattcg ttttgaaaat
     5521 gtataactaa taattctttt cgtgtgtaaa tccatgattg aagataagta acaccaacca
     5581 tttttcttag taggaatgta cgtaatgtca gctgcccatt tctcacagat cgtttcagta
     5641 gaaaagtcct gatttaagag attctctttt gaaatgatgg aatggcaaca gtttttttga
     5701 caaattttat aaggtgcaga acttctttcc gtatgctatt ccgattgtcc ttgacaatga
     5761 gcctcctcgg atgtgacgat cttcggcagg agctaacagt taaagttaga ctgcctcgct
     5821 agcgttagca catccgagct cattatcaag gattcgctgc gccaatctct ggttacggta
     5881 accaaccggt gtctcgtagc caagtgtacc gtgcaaccga aggtggttcc accaattgac
     5941 atagtcaaat aactccaaat ccaattgttg taaggtttca aatgtgtatt gatagacaaa
     6001 ttctactttc aacgacttat aagttgattc agctacggca ttatcaaaag gacagccttt
     6061 atgactcaat gatcgattga tgtcaaaagt tgttaataat tcatcaatag cttggttatc
     6121 aaactctttt ccacgatcag tatgaaaaat ctcaacctct gtcagagggg ttgtttgata
     6181 cggctaaatg cttttttact agaacggcat ctttatgttc tccacaagaa tagccgagaa
     6241 tttctcgatt gaacagatcc aaaatgaaac agacataatt ccattttttc ccgactcgta
     6301 cataagtcaa gtctgttacg atcgcttcta atgggttgtc tcttaagaat ttacgattca
     6361 atacgtttgt cgtttgcttc attgcaagta gaatgatgta ctttaaaata agcaacagta
     6421 tagctcgatt ttaatcctct atttttcatg attctactaa tttttttcgt cggctgatct
     6481 gaatgcctcg ttttgataag gctttttttt atttttcttg agccgtaggc ctttcggctg
     6541 cggataaatt cttcagcgac tacttcttca agttctgatt cgtctttctt tgttgatgga
     6601 taataatagg actgacgtga tagacctaat attctgcaca tcgctgatat agggtaaaga
     6661 tgcttattcg catcgattac ttgtctcttc gtccgaatat cagcgctgct tgctttaaaa
     6721 tatcattttc catttctaat tgctggtttc ttttacgtag ttctaacaat tctttttgtt
     6781 caggcgtaag attatctttt tctttgaatg aaccactcgt tttaaattgc tttacccatt
     6841 tgtcaaatgc tgaagccgtt agttcatatt ctcgaatgat ttctacacgt ggctttccag
     6901 ctaagtaaag attgacgatt tgttgcttga attcttgtga aaaagttctt cgtgttctct
     6961 tagacataaa aattcctcct ggtatgtttt cttctagtct acacacctta atttttctgt
     7021 ctagttaatt gtagcctatc catgatcggt ttattagacc tttgaggtct atatttttta
     7081 actacaatcg accttaaatt taattgtttc attagctttt ggacatgttt aagagatact
     7141 gaaatccctt cttttaataa aacttgatgg atctttgtgg caccatagcg ttgtttattc
     7201 gtaaaaaaaa ttgttgaaat cttctttttc aacttattat ttctgatttc tgtagctgtc
     7261 aacggtttat tcttataaaa ataatagaca cttctaggga tttctaataa ccgacacaac
     7321 aaagaaacag tgtaatcctt acaccatttt tcaataaatt gaacaagcga atttaggttt
     7381 tccgagagaa tatggctgcg gcttttttta agatttcaag ttcctcattt aaacgagcat
     7441 tttcttttct cagttcagct acttcttttc cagtcagtcc tgtggactga tcaggtaaat
     7501 ataaattctt ccatttgtaa atcgttgctt cagataagcc atattctttc gctaaccctc
     7561 gaacagaacg tccagtttga ttcagttcaa caatcatttg tttaaaattt tcttcatatc
     7621 gggtcataaa aattcatcct ctcgtagata agtttaattt attcttgtct ctacgaaaag
     7681 ctgtctaaga tttgatacta acaccaaatg ttgtataccc ccaaacaata cacatatttt
     7741 gaaggattag gattaataaa gctaaaaata tagtaagtat actaaatata tttactatat
     7801 ctagtatact tactatattt taatcttttg atcgtctatt atatataatc cgttcctcgt
     7861 cgtttaaagt atcaacatag gcgtccattg caagtttgac tatttcatac ataggaatat
     7921 cttttgcata actaagatcc cgaatggttt ttagtacggg aggatctact tgaactggtt
     7981 ttctttcttt cgcagtgtac tttcgttccc ctaactcgac attaatatca tctttaattg
     8041 aaatatcttc agcaccaatt ttcttcttat ctttgttcgt tggtaataat gccatttagt
     8101 ttaatctcct ttccaagagg taggatatga ttggccattg agtcagcata tttaggtgta
     8161 taatcaaatc cttcaatatc accatgttgt tcatagtatt caattctttc ttctaattct
     8221 gtaaagatgt ctgcaaaaat agcccaccat cttcgatcta cataatcatt taattggata
     8281 ccattctcac catacatttc taaccgatcc gagcctttaa caatgctttt aaaaatgttt
     8341 gattcaccat acttatcaat tgttttttga tagtttgcgt gttgagagga acgtcgcact
     8401 gagaaaagaa caggcaaaat tccaatgatt tgaagatgag actcttcaaa attcgtgaca
     8461 atcggaacta aaactttatt aataaaatct tctgttcctt ccattgcgta ccgttttaat
     8521 tcttgcattg caataatata atctgcagca gttaagaatg cacgaaccac attatctgtt
     8581 gatggtgggc agtcgataaa gatataatcg aaattttttc tcaattcttc taaaggttta
     8641 atgaaggcca atgatctctt acgacgatca ggatattttt cggacaagta ttcattaagt
     8701 tcttcagatc caggagagcc agccataata tataaatgtg gacttacttg agtaatacct
     8761 gaaacccagt cccctttttt gattgctgca gacatggatc gatcaaagct agtaatttga
     8821 tacgttgatc caatgttgga agtagtattt ctttgttggt cataatcaat tagcaacacg
     8881 tctttttcaa aaagttgact agaaacaatc gctaataaat cattgtctgt agtcttacca
     8941 atgccgcctt ttctacctgt aagcattaca acttttccta gtttatctga tgttttaaat
     9001 gcagtccgta agttttccat agttccctcc aatttcctat actaaatata tttagtatac
     9061 ttcatatact taatatattc gatatatcta atatactcca aattgataag aattcagact
     9121 ttataaggaa tatagtggat atactaaata tatttgctat attcagtata tcagatatat
     9181 tcaaaatgtc aaaatatatt tgtgcgtttt gcgttgacaa tttagagtga aaagaggtaa
     9241 acttaattct atagaaacca cagataattc actatttaca aagtagcaaa agcagtggta
     9301 ctctttaaac agaaaaatta cataaaaaaa agagacggaa ataattgatt ttagcagaat
     9361 caattatttc tgaatcacat ggttaggaca tgtgttcacg ctcgtctcaa gaatatgtaa
     9421 gtagctacca actacttata ttaaaagttg atcggatacc ttttccgatt gcctttattc
     9481 taccaaaaaa aggcgagcaa ctcaacagaa gaacgtttat ttagtgtact taaattaaat
     9541 attcttaaac actgaagaga gaacacatac tatggctgag attaaagcta aactgtgttc
     9601 tctttttgtt tgagaaagga cgttacaatc gatgttgagc gaaatttatt cagctattct
     9661 taaaaacggc ttacgtacat acaaatatcg aaacagcacc atgaaacctg caggtcatat
     9721 tgattccagt aggagtggtg ctatctttgc gtaccgtagc aagcaattaa tgaatgttgg
     9781 gcgtggtatg gtcattactt cggaagaagc tattttagag aacgaaaaga aactaacgca
     9841 ttggacacct aatacctatc gctttggtac ttatgcggat gattatcgaa cagtggttaa
     9901 aggtcattct gagaaaaact taagtcaaat caacacattt gtagttgata tcgattcaaa
     9961 agagaaccat caaggcgaaa ttattttagc ctgtttagat caagtgggct acatgccaac
    10021 gctaatttta gaatcagatc atggttatca agtttatttt gtactaaaga caccaggcta
    10081 tgtcacaaag aaaagcaatt tcaaggtgat cgatgtagca aaaaaaattt caaaaacaat
    10141 tcgtctacag ttggctgaaa aattgtccgg agtggatcta ggatgtaacc acttcggaat
    10201 tgctcgattc ccacaagaaa ataatattgt ttttaaagaa atgaactatc agtattcttt
    10261 tgctgactgg attcaatggt cgatgaagct tgaatcagat caaaaaacaa aagaagatcg
    10321 agaacgaaaa ctaattgtct ttcctgagaa aaaagaaatt cgtcaagtag atgaaccgtg
    10381 gttcgatatg ttacttcata aagcaaacat cgtcggcgga gaaggacgtc ttggtcgaaa
    10441 taatgtgatt ttcacgcttt ctttagctta ttactcttca ggttatgcat tggatacttg
    10501 cgaatacaat atgttcacat tcaacgaacg gttacaggaa ccgctatctg aagcagaatt
    10561 agtaaagatt gtacgtagtg cttattctgg taattatcaa gcagcaagca gagaattcat
    10621 tattgaattg tgccagcaat gggtttcacc aaccttaaaa aataatgatc tgttcattca
    10681 acgacgtggc tggtggaaat ttaaaaagcc acgagatcaa agaaaatata gtcataaaaa
    10741 tgaatggaaa gaagatctat tggcttatct aagcgaaaaa agtcttgcta gtgagcctta
    10801 tttgaacctg tcaaaaaaag aattggcttc acaactaacg atgcccaagc gaacacttga
    10861 taagatctta aatgagttac gagaggagaa taaagtcttc tacaaggtaa cacgaggaag
    10921 aaatggcgga atgatcattg cttcagtcaa aacgttgttc gcgagggtta ttcaactaag
    10981 taaagaaaag agactagatt attcaacaag gatcacagag gtatttggac tcaacgagag
    11041 cttagtagaa gagacattta gacaattatc aacccccttg aaccctctta aagaagtgaa
    11101 tctttttgaa gtagatacag gttgaaaaat aaaaggtgca aattgcctta tacaatatat
    11161 ctatgatact tctgtaggtg ttgctccctt tgcctagcta cgtgtaggtg gtatacttat
    11221 aactgcgtat gcagtcttat gtatcatgga ggaagttgta tgtcgttaaa aaattatgtc
    11281 tattcacatc ctgttaatgt gtttatcatt ggtcgtttgg gattatctgt ggaagtgttc
    11341 tgtgagcttt atggttttaa gcaggggact ttgtcttctt gggttaccag ggaaaaaaca
    11401 gttgcttctt tgccgattga gtttcttcat gggttgtcct tagcttctgg ggagactatg
    11461 gatcaggtat atgattgttt gtgtgtgtta gagcaggagt atattgagta taaaatagct
    11521 aacgagttaa gaaaaaggaa gaagtatatc cagtagtagt ttttatttac tcttaattgt
    11581 caaattaagt tagacaaatc atcaatactt acgtaactca aaaatacttc caaaggtgtt
    11641 cgataattca gtgattttct aggaatatta tttcgtttcg atgcgacaga ttgaataaaa
    11701 gattcatcta ctgaattgaa atccatagat ttcaataaac catctctacg taacaatccg
    11761 ttagaattct cgtttaggcc tctttgagac ggcgttcctg gatcagcgaa ataaatggca
    11821 atatcattgg cattactgat ctgtttccaa tttgaaaatt cctttccaca atcaaaagtg
    11881 atggatttga atagattttt cggtacagat tcaaaccaat gatttaattt gttttcaata
    11941 tcaatcgctt gtctaccaca cggtttcaat gtgatgataa cttttgataa tcgctcaact
    12001 aaggtaatta cagcactttt atgtttcaaa cctacgatag tgtcaccttc aaggtgacca
    12061 aactcatttg agaattggct ataatctttt tcacgttcat gaatagagcg acggaaagct
    12121 tgttttccac gtctttcttg atgtccattt ggtttacgct tgcctttcat cggtaagtca
    12181 gaaggattaa atagcccctt tttgaacata cgataaatgg tacgagcaga acaacgaata
    12241 ggaaacgctg cacgaccaac aatcacatcg ggtgtccatc cttgaacaac ctttctttga
    12301 atatattctg attgttcctc aggtaaaaca agcgggcgtc taccacagtt tgatttgttt
    12361 ttcttatact gttgatagta ctctaaggct gattttcctt gcttgaagaa caggtatact
    12421 ttatggatcg tttgtcttga acgattcaag aaatgcgcag ttttcgcaac agattgattt
    12481 attttgaaat aagactctat cattacaagt tcatccgttg taagatgggt ataggtcatt
    12541 tgtactcact ccttataatt ctttggacgg aactattttg agtgtagcac aaatgacttt
    12601 tttagttgtc tagcttaatt ttacaatcgg cgtacttaaa aaattggttc attgagcaac
    12661 ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa gaatttaggg aagaaatcga
    12721 aatgaaatcg gacgactgga ttgaacacac ctggcttcct ctgaaaagaa atagcaaacg
    12781 aaagaaattt tgggtattgc aattatggcc attaggaaaa ctttgtagtt acggggcacg
    12841 acttattctt gatttaaccg tttactcgat tggagtattt ggaacggctt attacttgat
    12901 aaatgatccc aataggattg cagcatcaga catttttaca gtgctaatga cgttgatttt
    12961 tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa tttaccaggc aaaaattaac
    13021 agcaggaaaa cttttcaaaa gcctattcga tttaattgtt gtaaatgcaa ttctagcgac
    13081 tttaataaaa caaattgaac aatctggaag aacaacaaaa ggagtgaagg attttttatt
    13141 gacgtttatt cactttgtaa atggacacat gtatttgttt gaagtgggta ttattctctt
    13201 ttttgtcggg tatgtatcca cgaaacttat taaacgttca attgaaaaag aaattgaaat
    13261 tgacctgatt ccgacacttc ttggttatga gtacaatgtt gattttggaa gatatgacga
    13321 attgatcaat acggatatcg ttcacacgag tatccatgaa tctactgtta agggcatcag
    13381 atctatttta aaaaatggcc aggaaatgaa agcaatagat cgaagaaaag agatctacaa
    13441 tttttatgaa gtcgaagcaa gcctacgata tggcatcgga aaattgtggt tagatggaag
    13501 taatattcgt caaaacaaac catacatttt ttggaacagt agtgatttag aacttccaat
    13561 gaaagataaa taattatgga gaatcgtttg aaaatgattc ctctatacag gattgtgctt
    13621 atagttggca tggagttaaa aaccttaaag aaggagcagt tattcaaagt gaaagaacgt
    13681 cataatcagt tactatgtag taaaacgggt acgatgaaca ttgaataccg aacagaatat
    13741 ttagaattaa cgaatcaaac tgattttttg ttgattcgaa agcaacaaga gcaagaaaaa
    13801 aattaatggc tacttatagc caattgattg atgaatggga acaattcaag gaatctaaaa
    13861 ttccagaacg tctctaaaag cctttatttg taaagagcat aaggaaacgt gggaacactt
    13921 tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt ctcaaaaata aatgtgggaa
    13981 caaaatattt aaaaatagga tagtgaaagg atagtgaagg atagtaaaag gagggtagta
    14041 ggatagcaaa aaggataggc tccttttcgc tatccttttt aaattggagg aattaatatg
    14101 gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag aaattgatcg aagatgtgac
    14161 gaacggttct gttgcaaagt tttaaatcta ctatcaaata aggtagaata atagaaaaag
    14221 atggcaggag gaatgacgat gaatcatttt aaaggaaagc aatttcagca ggatgtgatt
    14281 attgtagccg tgggctacta tcttcgttat aaccttagct atcgtgaagt tcaagaaatc
    14341 ttatatgatc gtggcattaa cgtttctcat acgacgattt atcgttgggt gcaagaatat
    14401 ggcaaactac tctatcaaat ttggaaaaag aaaaataaaa aatcctttta ttcatggaaa
    14461 atggatgaaa cgtacatcaa aattaaagga aaatggcatt atttgtatcg agccatcgat
    14521 gcagatggtt taaccttgga tatttggtta cgtaaaaaac gggacacaca agcagcctat
    14581 gcttttctta agcggttagt gaagcagttt gatgaaccga aggttgtagt cacagataaa
    14641 gccccctcta ttacaagtgc ctttaagaaa ctaaaagaat acggctttta tcaagggaca
    14701 gaacatcgta ccattaaata cctgaataat ttgattgaac aagactatcg tccagtaaag
    14761 agacgcaata aattctatcg aagtttacgc actgcctcta ccacgattaa aggcatggaa
    14821 gccattcgag gattatataa gaaaacccga aaagaaggca ctctcttcgg gttttcggtc
    14881 tgtactgaaa tcaaggtatt attgggaatc ccagcttaaa tcatagatac cgtaagggat
    14941 tttattcttt atttaaaact ttgcaacaga accagcgaag taataaagta agtacctggt
    15001 ctaaaaacgg cattacagag cataaaggct aatgacaaaa agttttatcc atgtataaga
    15061 acgtattttt tgaaatgctt gagcgaatta ttcaattaga aaacgaaaaa gaatagaatc
    15121 acaaatcaca agtgattaat cacaaatcac ttgtgatttg tgattgttga tgataaaata
    15181 agagtaagaa gaaatagaaa gaagtgagtg attgtgggaa atttaggcgc acaaaaagca
    15241 aaacgaaatg atacaccaat ccagtgcaaa aaaagatata atgggagata agacggttcg
    15301 tgttcgtgct gacttgcacc atatcataaa aatcgaaaca gcaaagaatg gcggaaacgt
    15361 aaaagaagtt atggatcaag ccttagaaga atatatacgg aaatatttac ctgacaaact
    15421 ttaaaaggag tgaaaatgaa atggcagtta cgtatgaaaa aacatttgaa atagagatca
    15481 ttaacgaatt atcggcaagc gtttataatc gagtattaaa ctatgttttg aaccatgaat
    15541 taaataaaaa tgactctcaa ttattggaag tcaatttatt aaaccaatta aagcttgcaa
    15601 aacgtgtaaa tctttttgat tattctttag aagaattaca agccgttcat gagtattggc
    15661 ggtcaatgaa tcgttactca aaacaagttt tgaataaaga gaaagtggct taatatggca
    15721 aatatagtca attttactga caaacaattt gagaatcgct taaatgataa tttagaagaa
    15781 ttgattcaag gaaaaaaagc ggttgaatcg ccaaccgctt ttttacttgg tgggcaacca
    15841 gggtcaggga aaaccagttt gcgatcagca atttttgaag aaacacaagg gaatgttatt
    15901 gtcattgata atgatacctt taaacaacag caccctaatt ttgatgaact agtgaaactt
    15961 tatgaaaaag acgtagtaaa acacgttacc ccttattcta atcgcatgac agaagcgatc
    16021 ataagccgtt tgagcgatca agggtataat ttggtgatcg aaggtacagg acgaacaaca
    16081 gacgttccta ttcaaaccgc aacaatgctt caagccaaag gttatgaaac aaaaatgtat
    16141 gtcatggcag tacctaaaat caactcatat ttaggaacaa ttgaacgata tgaaaccatg
    16201 tatgcagatg atccaatgac agccagggca acaccaaaac aagcgcatga tattgttgtc
    16261 aaaaacttac cgaccaattt agaaaccctt cataaaacgg gcttatttag cgatataagg
    16321 ctatataaca gagaaggagt aaaactctat tcaagtttgg aaacaccttc tattagtcca
    16381 aaagaaacct tagaaaaaga attgaatcgt aaagtatcag ggaaagaaat tcaaccgact
    16441 ttagaacgaa tagagcaaaa aatggttcta aataaacacc aagagacacc tgaatttaaa
    16501 gcaattcaac aaaaattgga aagcttgcag ccacctacac caccaatacc caaaacacct
    16561 aaacttccag gtatttaaac cttaaaaaag gaaaagagta gttaccaaaa aacggtaact
    16621 actctttttt tataaaaaca tttcctcatg ttggggtccc gagcgcctac gaggaatttg
    16681 tatcgataag aaatagattt aaaaatttcg ctgttttttt ctaatttcat tttgaggtga
    16741 attaattggt agttattaga ggtgccctat acctaataat ttatctacat tccctttagt
    16801 aacgtgtaac tttccaaatt tacaaaagcg actcatagaa ttatttcctc ccgttaaata
    16861 atagataact attaaaaata gacaatactt gctcataagt aacggtactt aaattgttta
    16921 ctttggcgtg tttcattgct tgatgaaact gatttttagt aaacagttga cgatattctc
    16981 gattgaccca ttttgaaaca aagtacgtat atagcttcca atatttatct ggaacatctg
    17041 tggtatggcg ggtaagtttt attaagacac tgtttacttt tggtttagga tgaaagcatt
    17101 ccgctggcag cttaagcaat tgctgaatcg agacttgagt gtgcaagagc aaccctagtg
    17161 ttcggtgaat atccaaggta cgcttgtaga atccttcttc aacaatcaga tagatgtcag
    17221 acgcacggct ttcaaaaacc acttttttaa taatttgtgt gcttaaatgg taaggaatat
    17281 tcccaacaat tttatacctc tgtttgttag ggaattgaaa ctgtagaata tcttggtgaa
    17341 ttaaagtgac acgagtattc agttttaatt tttctgacga taagttgaat agatgactgt
    17401 ctaattcaat agacgttacc tgtttactta ttttagccag tttcgtcgtt aaatgccctt
    17461 tacctgttcc aatttcgtaa acggtatcgg tttcttttaa attcaattgt tttattattt
    17521 ggttgagtac tttttcactc gttaaaaagt tttgagaata ttttatattt ttgttcatgt
    17581 tatcactcct gaagtgatta catctataaa caaatacaga agttaaacga tttgtttgta
    17641 attttagtta tctgtttaaa aagtcataag attagtcact ggtaggaatt aatctaacgt
    17701 atttatttat ctgcgtaatc actgttttta gtctgtttca aaacagtaga tgttttatct
    17761 acataacgca tttggaatac caacatgacg aatccctcct tcttaattac aaatttttag
    17821 catctaattt aacttcaatt cctattatac aaaattttaa gataatgcac tatcaacaca
    17881 ctcttaagtt tgcttctaag tcttatttcc ataactttag ggttaaccat acgcaagacc
    17941 aatcactctc ggacaatact catgatttta atgataaaat ccatgttaaa cccatagata
    18001 agaaatacac ctgcaataac cgttacctgt ttgtgccaat ttaaaaatgc accacttttt
    18061 tataattaaa acggttgaaa actgtaccac taataactca caatagagag atgtcaccgt
    18121 caagttaaat gtacaaaata acagcgaaat ttttaaatct atttcttatc gatacaaatt
    18181 cctcgtaggc gctcgggacc cctatctagc gaacttttag aaaagatata aaacatcaga
    18241 gtatggacag ttgcggatgt acttcagaaa agattagatg tctaaaaagc ttgtagttaa
    18301 agctttttag acatctaaat ctaggtacta aaacaattca tccagtaaaa tataatattt
    18361 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
    18421 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
    18481 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
    18541 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
    18601 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
    18661 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
    18721 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
    18781 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
    18841 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
    18901 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
    18961 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
    19021 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
    19081 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
    19141 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
    19201 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
    19261 aagttggcgt ataacatagt atcgacggag ccgattttga aaccacaatt atgatagaat
    19321 ttacaagcta taaggttatt gtcctgggtt tcaagcatta gtccatgcaa gtttttatgc
    19381 tttgcccatt ctatagatat attgataagc gcgctgccta tgccttgccc cctgaaatcc
    19441 ttacatacgg cgatatcttc tatataaaag ataacccgtg gtgctagtta attttttcct
    19501 agaggaaaat gtctaaacat gttggtaaat caacaaaaaa acgcatatag tagtctcaaa
    19561 actcccaaga aatgaggact acatatgcgt tcatcaaaat atactgaaca ttatacagat
    19621 acttttataa cattcaagga aattatgaga actgtttcta acatatacca caattgtgta
    19681 ccagataaga ttaaaaaccg aagaaatact gatcaactga agcaacgcga tacagtgatt
    19741 attgcttgtg ttatatgggg tataattaat ggctatacta gccaaagagc cacgtataga
    19801 gcggtttgtt ccgtcttatt tcctaatggt gactttccta gtaggagtcg attcactcgc
    19861 ttaagttcaa acttagctta cactatcaag attattcgat actttttcat caagaagctc
    19921 accaaaggtg aactagtcgg aattatagat agttttccta gtccattatg taaaccagtt
    19981 agaaatagac aagcaaaact attgaatcaa atagccaaag ttggctataa ctcaacaaaa
    20041 aaatcttatt tttatggtct taaaattcat atgatcgtta ctaagactgg ctttccgatt
    20101 acttactcaa tcacgaatcc aggtgtccat gacgtgaaag tgttagagac tttatcggaa
    20161 gaagcgaatc ttcccaatat cctaggggac aaagggttct gttgcaaagt tttaaataaa
    20221 gaataaaatc ccttacggta tctatgattt aagctgggat tcccaataat accttgattt
    20281 cagtacagac cgaaaacccg aagagagtgc cttcttttcg ggttttctta tataatcctc
    20341 gaatggcttc catgccttta atcgtggtag aggcagtgcg taaacttcga tagaatttat
    20401 tgcgtctctt tactggacga tggtcttgtt caatcaaatt attcaggtat ttaatggtac
    20461 gatgttctgt cccttgataa aagccgtatt cttttagttt cttaaaggca cttgtaatag
    20521 agggggcttt atctgtgact acaaccttcg gttcattaaa ctgcttcact aaccaagcat
    20581 aggctgcttg tgtgtcttgt ttttacgtaa ccaaatatcc aaggttaaac catctgcatc
    20641 gatggctcga tacaaataat gccattttcc tttaattttg atgtacgttt catccatttt
    20701 ccatgaataa aaggattttt tatttttctt tttccaaatt tgatagagta gtttgccata
    20761 ttcttgcacc caacgataaa tcgtcgtatg agaaacgtta atgccacgat catataagat
    20821 ttcttgaact tcacgatagc taaggttata acgaagatag tagcccacgg ctacaataat
    20881 cacatccaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtca ttcctcctgc
    20941 tatctt
//
