LOCUS       AP026598                6028 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium JHP38 plasmid pJHP38_4 DNA, complete sequence.
ACCESSION   AP026598
VERSION     AP026598.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466806
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JHP38"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJHP38_4"
                     /geo_loc_name="Japan"
                     /collection_date="2002"
     gene            complement(744..914)
                     /locus_tag="EfmJHP38_34300"
     CDS             complement(744..914)
                     /locus_tag="EfmJHP38_34300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62492.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(930..1466)
                     /locus_tag="EfmJHP38_34310"
     CDS             complement(930..1466)
                     /locus_tag="EfmJHP38_34310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDP62493.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1487..2431)
                     /gene="rep"
                     /locus_tag="EfmJHP38_34320"
     CDS             complement(1487..2431)
                     /gene="rep"
                     /locus_tag="EfmJHP38_34320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP62494.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep"
     gene            complement(3275..3964)
                     /locus_tag="EfmJHP38_34330"
     CDS             complement(3275..3964)
                     /locus_tag="EfmJHP38_34330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62495.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4204..5118)
                     /gene="mobA"
                     /locus_tag="EfmJHP38_34340"
     CDS             complement(4204..5118)
                     /gene="mobA"
                     /locus_tag="EfmJHP38_34340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDP62496.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="mobA"
     gene            complement(5100..5489)
                     /gene="mobC"
                     /locus_tag="EfmJHP38_34350"
     CDS             complement(5100..5489)
                     /gene="mobC"
                     /locus_tag="EfmJHP38_34350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDP62497.1"
                     /translation="MGLMSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC"
     Typing          complement(1488..2431)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4208..5118)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttacaaaga cattcattgc atcaacagca ctactaaaag catttgtaat aacccatgtt
       61 ccaactccac caataggaat aaaagatgca atttgagcag gagtctttag cttattataa
      121 ttttttttca aatctctaca cattttatta gtaacataat agttcatttt atagcaagta
      181 aatccacgat gattatgatt atatcgtgaa cttgcagctt ccacttttat acctctagca
      241 ctaaaaggaa acagatttaa cggagtactt gctccaacaa tcataccgaa caaaaataga
      301 caagtaataa ttttaataat tttatttttc attgttactt cctccttaga tataaaatcc
      361 taggaaagcg ctttctgtat atatttagaa taatcagaaa attaaatatt gtcaagaggg
      421 tttattttat aaaacgtgtt attttaaagg ttagtagaca taatgtgtag tataccaagt
      481 agcccttgta tcaaccgata caggggctca ctttgtgatt tttaattggt ctaggtcaaa
      541 agctcttttt aaggcttgat tttctgcagt tgcttctttc cgtttctttg tgaattttta
      601 ccagtcattt tttactggat tgttattttt ataaaatatg ataaaatttt ggtagcttta
      661 tttataagcg ttgcgcgttg ctttttcaag aataaactct cattattttt ttaactggct
      721 gctcgaaaga gcagtctttt ttattattta tccgtttctt ttacaaacaa ttcttcgtta
      781 ggactgttgt aataatttgg attcaatttc aattcaccat gtttattccc tttattgatc
      841 actgacaata acatcggact gaagcctaga attcctagta aaatagaacc gaatagaaga
      901 ttttttcttg tcatgaatat tccctccagt tatttccaaa gttgccacca tttttgcttc
      961 ttctttgtgt gtttttctat cgattctgtt tcttcagaaa gaatagtagt tgattcatct
     1021 tctggagttt cttccgtata aactaattgt aactgttctt gcagtttttc gatctgttga
     1081 tttgcctgta gggtgagctg ttgttgctgg tcgagtaatt tttgcatatt agaattattt
     1141 tgctctttaa gggaaagttg ttctcgtaaa aattcatttt cttcacgcaa cttatctagc
     1201 aactcgttta caaccacgtt tttgcttgat atataaatgt ttttatcgtt gtctttttca
     1261 tactcatgca acctatctag taacctttgg ttacctaagt tgctagcaag ttgcaatact
     1321 ccagaacgca atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat
     1381 ttttggatat gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt
     1441 attgtaaagg tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat
     1501 tatctaaatc agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt
     1561 tttcaagagc ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc
     1621 gtttcttctg tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt
     1681 tatcagctaa agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt
     1741 cgtctgcttt tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc
     1801 gtccttttcc gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac
     1861 gtataggttt tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta
     1921 ataattcaag aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta
     1981 acagacgaaa catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat
     2041 agcgtaccca ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat
     2101 ctacgtaagg attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat
     2161 cgtaagaaag cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag
     2221 ttgcttttaa atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt
     2281 ttagttggtc aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga
     2341 tggaaaaaaa caaattcatt tcaaccggtg taaattttct taaaggaatt gtatttaatt
     2401 catggtggta ttttacaatc tcattagcca tataatcacc tcaatccaat tatgaatgac
     2461 attttttaaa tgtcaagaca ttcatatttg acgttcaaac agacaaaatg tccttcataa
     2521 acagacaaaa tgtccttcat aaacagacaa aatgtccttc ataaacagac aaaatgtcct
     2581 tcataacatt ctgtatccat tggtagagta aggacacagc ttacggtaaa gaggtttaaa
     2641 aagaggttta aaaagagata taaaaagagg ggggctaatt ttcccgccta aaaaccactt
     2701 cttttttctt taaacaggtt cgcgcatttt tcaaaactgc caatccaaaa ccttgcttcc
     2761 tacggtcgcc aaaaaggtgt gatcgctacg ctctcacatt gtactgcgca gtcgcctaaa
     2821 ggctcctttg ctaaaaacat gttggtaaag aggcctttga gcggttgtga atgcttatct
     2881 aagccgtttt aagacagtct tggataaaaa tccttatcta gctttaaagc gccttacagg
     2941 cggtcagggc aagccctaac aaccctaaaa taagcgatac ttaaaaacac cacacaagtt
     3001 taatttttta agatataata acttgtcgcc tatccgacag tcttgcgact ggaaggaggt
     3061 gtatctaatg tttgaattgt tcatttgccc ccttcttata gaagtttgtg tctctttatt
     3121 cagttattgg ttgaataacc gaaacaagaa gtaaaatcaa taaaggcgac atcaacctga
     3181 aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtatctaatg tttgatcatt
     3241 tgcatgttta gtttaacatg attgccgatc aaattcaatt ttgctgttgc tgatctaacc
     3301 attccttgac actttctaga taatttaatg ccgtttcttt tttcgttgga ctttgtatgt
     3361 tagttgcttg ttgaaacagg ttttcgtagc gttcatatgt gacagattgt tgtttttctt
     3421 ttttttcttc ttcactggca aaccaagggt ttgtgtctcc aaatagatca ctacaggctt
     3481 cttctatctt tgtatctttt acatattgca cgtccttttt tacttgttca tatttttctt
     3541 ggtattcttt ttgcggttct tctggcaaat tccgataaag tcgagtgagt gtttccatat
     3601 ttttatccgt caaacgcttt tcttgcttca caaaaatatc attcatgtcc tcataacaag
     3661 cggtaaattt ttctattttt tgtgctaagg cttgctcttt tttcttttta ttacgtgctg
     3721 attcatactt ttgagtgaca tttcctaata aaatacttct agaaacgcgc ttattgccct
     3781 ctaaaacgcc taataagccg cttgaaacga ttatcaagga aaatatacag ccaagcaaga
     3841 aaaatcgctt aaatcgaatt ttagagcctt ttaatgaata tcttgtttgg aaaattttat
     3901 agatgagcca aatacttcct gaaatactaa caaaaatgag tagatagggt aaataaaatg
     3961 ccattttagc ctccccatac taaccaaaat gcgaacagtg tcaggactaa gcaacataag
     4021 ggggcaagaa aatccattga tcgttgaatg aacgcctttt tttgttcttt atttcgctga
     4081 tctaatagct cttgatggag ttttttaact tgtaaatcaa tcgtagtttg tatcctttct
     4141 tgtgtgtagg gagcaagaaa agcacgttgt ttctttattt cgttttctat gtcttttaac
     4201 gttttagagc gataattctt gttggtggtc tgttcctctt tcaacgtgtt gttcatggtt
     4261 tcttcgagcc ggtctaattt ccggttgttc aggcgtaaga gttcgttgtt ctcttcgtac
     4321 ttcttctgca gtttcgcgaa tgctagctcg agctcttcgt agctcgttgg ttcgctgttg
     4381 gttataggtt cgttctcgtc tgtccagctc atgtaaaatc gtctcctttt cgtagtcctt
     4441 tcctagtctg ttggatcgta cttttttctt tttgtggatc aattcgtaag tgaaattttt
     4501 cccgcgattg aagcaatgaa tgccgaatgt tttgagggaa tcacaaaatt ggtcataggt
     4561 tctggttttc ttgtttttca tggtttgatc aatcgcaaac cgaatttcat ctttccaaac
     4621 aggttcattt ttttctttta ttttgatttc agccatggtt cgtttttctt ctttttttcc
     4681 tcgttcagtg atacttaaac catgagttaa acaaatttcg tcgttgatat cgtaacactt
     4741 atctaggaat gcttgaccat gtgcttgaaa cttttttcct gtttctaaat tgaccgaatt
     4801 aatgacaatg tgattgtgga tatgctcttt atctgtgtgc gtgtaaatcg cgatttggta
     4861 ttctggcgcg attttttcag cgtattcttt ccctagttgg ttggcttttt cagcggtaat
     4921 ttctcctggt cgaaaggact gaatcaataa atgtgcttgt acacgatcgt cttttccata
     4981 aagcatgcgt atttgtttca tttcactttt ggcataatca atgtcacagt ttattcctgt
     5041 tttgacggtt gctcgtggtt ctgcgtaatt gattgctcgg ctacatgaag tggttcgact
     5101 tactttaact gttgccatat cttttgtagc tccttttgca tgatttttag gttttcggat
     5161 aaacgttgag cgtcaccagg agcaacgtca tgttggtgtg tattgcacca cttagcaatt
     5221 tgattgatat tccctccagc tttggctaaa tcgcgcgcga ttgcctgggc aatgtctggc
     5281 gccactttcg gtgtcaccag acgcgcgttt tgtgccttgg ctttcacgaa agccggcaca
     5341 ctcatttgca aagcttcagc tgactgcttc agctttgcaa attcggattc gctcactcga
     5401 aaactgattt gttttggctc ggatcgatag gtatattttt tagaaggatc gctagccaaa
     5461 ttttgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga cgtatcgttc
     5521 gcaataccat tatacctaaa aaggtacaat gagtaaagct cactaaaagg gtttggcaag
     5581 ctctatattt gctagccaaa tacctccgta tttggtgcaa ataaccgctt gtcaaagggg
     5641 cgcaagctcc cccttgaatc cccctcaaaa cccacgctcc acaatacaaa aagtggctag
     5701 aaaatgctag ccactttttg taaaatttag tatgatttat ttttagctat ttagagtttt
     5761 tatttccgta aatacttcta aaaattgcta agataaatgc tataggaatt agataaagaa
     5821 tacttttttg taagcttata ttgaaaatta gcctaaatat gaagaacata gtgattaaaa
     5881 ctattattga aataattaca gctaaaatct ttttattcat tctgattatt ttattacata
     5941 acgagcgtaa tcattgtatt gatatgatgt tgtattgcta aagtgagcat tatatgttaa
     6001 ttgtacacct tttccttgat ttgcagct
//
