LOCUS       AP026597                6087 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium JHP38 plasmid pJHP38_3 DNA, complete sequence.
ACCESSION   AP026597
VERSION     AP026597.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466806
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6087)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6087
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JHP38"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJHP38_3"
                     /geo_loc_name="Japan"
                     /collection_date="2002"
     gene            complement(877..1035)
                     /locus_tag="EfmJHP38_34200"
     CDS             complement(877..1035)
                     /locus_tag="EfmJHP38_34200"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     1060,1159"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62482.1"
                     /translation="MNAKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(2627..2758)
                     /locus_tag="EfmJHP38_34210"
     CDS             complement(2627..2758)
                     /locus_tag="EfmJHP38_34210"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62483.1"
                     /translation="MATVMATVKVSRTTYVAPGDAQRLSENLKIMQKELQKIWQQLK"
                     /name="hypothetical protein"
     gene            complement(2728..3024)
                     /locus_tag="EfmJHP38_34220"
     CDS             complement(2728..3024)
                     /locus_tag="EfmJHP38_34220"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     3168,3108,3063"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62484.1"
                     /translation="MSESEFAKLKQSAEALQMSVPAFVKAKAQNARLVTPKVAPDIAQD
                     LAKAGGNINQIAKWCNTHQHDVAPGDAQRLSENLKIMQKELQKIWQQLWQQLK"
                     /name="hypothetical protein"
     gene            complement(3444..3878)
                     /locus_tag="EfmJHP38_34230"
     CDS             complement(3444..3878)
                     /locus_tag="EfmJHP38_34230"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62485.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     gene            complement(4430..4642)
                     /locus_tag="EfmJHP38_34240"
     CDS             complement(4430..4642)
                     /locus_tag="EfmJHP38_34240"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62486.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNNKGNK
                     HGELKLNPNYYNSPNEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(4658..4813)
                     /locus_tag="EfmJHP38_34250"
     CDS             complement(4658..4813)
                     /locus_tag="EfmJHP38_34250"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 4955;
                     possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62487.1"
                     /translation="MQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTKKKQK
                     WWQLWK"
                     /name="hypothetical protein"
     gene            complement(4856..4984)
                     /locus_tag="EfmJHP38_34260"
     CDS             complement(4856..4984)
                     /locus_tag="EfmJHP38_34260"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 4955;
                     possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62488.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGY"
                     /name="hypothetical protein"
     gene            complement(5202..5507)
                     /locus_tag="EfmJHP38_34270"
     CDS             complement(5202..5507)
                     /locus_tag="EfmJHP38_34270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62489.1"
                     /translation="MFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIR
                     QELTAIFKGLTELELLQQIKQLEKEKKALEKNLRSKKQTTLTNMHQPKKTKIKHWL"
                     /name="hypothetical protein"
     gene            complement(5440..5724)
                     /locus_tag="EfmJHP38_34280"
     CDS             complement(5440..5724)
                     /locus_tag="EfmJHP38_34280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 5785"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62490.1"
                     /translation="MFRLLKQYRQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIK
                     PIRQELTAIFKGLTIKKKYGKGRVCKDYVSSVKAIQNQRFRIFLQRRLS"
                     /name="hypothetical protein"
     gene            complement(5648..6019)
                     /locus_tag="EfmJHP38_34290"
     CDS             complement(5648..6019)
                     /locus_tag="EfmJHP38_34290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP62491.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSLR
                     FGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRMGTLFFTTIQRATKWLCKDYVSSVK
                     AIQTIQNQRFRIFLQRRLS"
                     /name="hypothetical protein"
ORIGIN
        1 catttcaacc ggtgtaaatt ttcttaaagg aattgtattt aattcatggt ggtattttac
       61 aatctcatta gccatataat cacctcaatc caattatgaa tgacattttt taaatgtcaa
      121 gacattcata tttgacgttc aaacagacaa aatgtccttc ataaacagac aaaatgtcct
      181 tcataaacag acaaaatgtc cttcataaac agacaaaatg tccttcataa cattctgtat
      241 ccattggtag agtaaggaca cagcttacgg taaagaggtt taaaaagagg tttaaaaaga
      301 gatataaaaa gaggggggct aattttcccg cctaaaaacc acttcttttt tctttaaaca
      361 ggttcgcgca tttttcaaaa ctgccaatcc aaaaccttgc ttcctacggt cgccaaaagt
      421 gtgtgtgatc gctacgctct cacattgtac tgcgcagtcg cctaaaggct cctttgctaa
      481 aaacatgttt ggcctttgaa atgcttatct aagccgtttt aagacagtct tggataaaaa
      541 gtcttggata aaatccttat ctagctttaa agcgccttac aggcggtcag ggcaagccct
      601 aacataaaat aagcgatact taaaaacacc acacaagttt aatgtcgtta agataacata
      661 acttgtcgcc tgcctatccg acagtcttgc gactggaagg aggtgtatct aatgtttgaa
      721 ttgttcattt gcccccttct tatagaagtt tgtgtctctt tattcagtta ttggttgaat
      781 aaccgaaaca agaagtaaaa tcaataaagg cgacatcaac ctgaagccca tgtttgatca
      841 tttgcatgtt tagtttaaca tgattgccga tcaaattcaa ttttgctgtt gctgatctaa
      901 ccattccttg acactttcta gataatttaa tgccgtttct tttttcgttg gactttgtat
      961 gttagttgct tgttgaaaca ggttttcgta gcgttcatat gtgacagatt gttgtttttc
     1021 tttttttgcg ttcatatgtg acagattgtt gttttctttt tttcttcttc actggcaaac
     1081 caagggtttg tgtctccaaa tagatcacta caggcttctt ctatctttgt atcttttatc
     1141 ttttgcacgt cctttttact tgttcatatt tttcttggta ttctttttgc ggttcttctg
     1201 gcaaattccg ataaagtcga agtagtgttt gcatattttt atccgtcaaa cgcttttctt
     1261 gcttcacaaa aatatcattc atgtcctcat aacaagcggt aaatttttct atttctattt
     1321 tttgtgctaa gtctttttct ttttcttttt attacgtgct gattcatact tttgagtgac
     1381 atttcctaat aaaatacttc tagaaacgcg cttattgccc tctaaaacgc ctaatattga
     1441 gccgattatc aaggaaaata tacagccaag caagaaaaat cgcttaaatc gaattttaga
     1501 gccttttaat gaatatcttg tttggaaaat tttatagatg agccaaatac ttcctgaaat
     1561 actaacaaaa atgagtaggc aagaaaatcc attgatcgtt gaatgaacgc ctttttttgt
     1621 tctttatttc gctgatctaa tagctcttga tggagttttt taacttgtaa atcaatcgta
     1681 gtttgtatcc tttcttgtgt gtagggagca agaaaagcac gttgtttctt taagttttta
     1741 acttgtaaat caatcgtagt ttgtatcctt tcttgtgtgt agggagcaag aaaagcacgt
     1801 gttgttcatg gtttcttcga gccggtctaa tttccggttg ttcaggcgta agagttcgtt
     1861 gttctcttcg tacttcttct gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt
     1921 tggttcgctg ttggttatag gttcgttctc gtcttcttct gcagtttcgc gaatgctagc
     1981 tcgagctctt cgtagctcgt tggttcgctg ttggttatag gttcgttctc gtctgtccag
     2041 ctcatgtaaa atcgtctcct tttcgtagtc ctttcctagt ctgttggatc gtacttttct
     2101 ttttgtggat caattcgtca agtgaaattt ttcccgcgat tgaagcaatg aatgccgaat
     2161 gttttgaggg ttgagcaaaa ttggtcatag gttctggttt tcttgttttt catggtttga
     2221 tcaatcgcaa accgaatttc atctttccaa acaggttcat tttttctttt attttgattt
     2281 cagccatggt tcgtttttct tctttttttc ctcgttcagt gatacttaaa ccatgagtta
     2341 aacaaatttc gtcgttgata tcgtaacact tatctgtgta agcttgacca tgtgcttgaa
     2401 actttttcct gtttctaaat tgaccgaatt aatgacaatg tgattgtgga tatgcgcgga
     2461 tatgctcttt atctgtgtgc gtgtaaatcg cgatttggat tttttcagcg tattctttcc
     2521 ctagttggtt ggcttttggc ataatcaatg tcacagttta ttcctgtttt gacggttgct
     2581 cgtggttctg cgtaattgat tgctcggcta catgaagtgg ttcgacttac tttaactgtt
     2641 gccatatctt ttgtagctcc ttttgcatga tttttaggtt ttcggataaa cgttgagcgt
     2701 caccaggagc aacgtaagtg gttcgactta ctttaactgt tgccataact gttgccatat
     2761 cttttgtagc tccttttgca tgatttttag gttttcggat aaacgttgag cgtcaccagg
     2821 agcaacgtca tgttggtgtg tattgcacca cttagcaatt tgattgatat tccctccagc
     2881 tttggctaaa tcctgggcaa tgtctggcgc cactttcggt gtcaccagac gcgcgttttg
     2941 tgccttggct ttcacgaaag ccggcacact catttgcaaa gcttcagctg actgcttcag
     3001 ctttgcaaat tcggattcgc tcactcgaaa actgatttgt tttggctcgg atcgataggt
     3061 atatttttag aaggatcgct agccaaattt tgattttgtt cgctcgctag ccaaatcttt
     3121 cagtacgtcc tacggacgta tcgttcgaaa actgaccatt atacctaaaa ggtacaatag
     3181 gtaaattttt agaaaaaggg cactaaaagg gtttggcaag ctctatattt gctagccaaa
     3241 tacctccgta ttagtacgtc aacggacgca agctcccctt gaatcccatt caaaaaaaag
     3301 gtacaaatac aaaaagtgaa gaatactttt ttgtaagctt atattgaaaa ttagcctaaa
     3361 tatgaagaac atagtgatta aaactattat tgaaataatt acagctaaaa tctttttatt
     3421 catgagatag tttcactttc tgattatttt attacataac gagcgtaatc attgtattga
     3481 tatgatgttg tattgctaaa gtgagcatta tatgttaatt gtacaccttt tccttgattt
     3541 gcagctctta caaagacatt cattgcatca acagcactac taaaagcatt tgtaataacc
     3601 catgttccaa ctccaccaat aggaataaaa gatgcaattt gagcaggagt ctttagctta
     3661 ttataatttt ttttcaaatc tctacacatt ttattagtaa cataatagtt cattttatag
     3721 caagtaaatc cacgatgatt atgattatat cgtgaacttg cagcttccac ttttatacct
     3781 ctagcactaa aaggaaacag atttaacgga gtacttgctc caacaatcat accgaacaaa
     3841 aatagacaag taataatttt aataatttta tttttcattg ttacttcctc cttagatata
     3901 aaatcctagg aaagcgcttt ctgtatatat ttagaataat cagaaaatta aatattgtca
     3961 agagggttta ttttataaaa cgtgttattt taaaggttag tagacataat gtgtagtata
     4021 ccaagtagtt agatataaaa tcctaggaaa gctaggaaag cgctttctgt atatatttag
     4081 aataatcaga aaattaaata ttgtcaagag ggtttatttt ataaaacgtg ttattttaaa
     4141 ggttagtaga cataatgtgt agtatacgaa gtagcccttg tatcaaccga tacaggggct
     4201 cactttgtga tttttaattg gtctaggtca aaagctcttt ttaaggcttg attttctgca
     4261 gttgcttctt tccgtttctt tgtgaatttt taccagtcat tttttactgg attgtttatt
     4321 tttataaaat atgataaaat tttggtagcg ctttataagc gcgttgcatt ttcaagaata
     4381 aactctcatt atttttttaa ctggctgctc gaaagagcag tcttttttat tatttatccg
     4441 tttcttttac aaacaattct tcgttaggac tgttgtaata atttggattc aatttcaatt
     4501 caccatgttt attcccttta ttatttggat tcaatttcaa ttcaccatgt ttattccctt
     4561 tattgatcac tgacaataac atcggactga agcctagaat tcctagtaaa atagaaccga
     4621 atagaagatt ttttcttgtc atgaatattc cctccagtta tttccaaagt tgccaccatt
     4681 tttgcttctt tttagttgat tcatcttctg gagtttcttc cgtataaact aattgtaact
     4741 gttcttgcag tttttcgatc tgttgatttg cctgtagggt gagctgttgt tgctggtcga
     4801 gtaatttttg catattagaa ttattttgcg ttgctggtcg agtaattttt gcatattaat
     4861 aaccacgctc atttttagct atcatggaag aatctaattt ttggatatgt ttcgatattg
     4921 cttgcctact catcttaaaa agatctgaca attcctttat tgtaaaggtt tcttgtggtt
     4981 gcatactggt tgcctcctag gttgctactg gaataaatta tgttgctagc aagttgcaat
     5041 actccagaac gcaatacaac cttataacca cgctcatttt tagctatcat ggaagaatct
     5101 aatttttgga tatgtttcga tatgttcaag ttctcgtttc ttctgtttga ttttttcgtt
     5161 aattgttgta gcgggttctt cgtattcttt ttgtttatca gctaaagcca atgctttatt
     5221 ttggtcttct ttggttggtg catgtttgtt aaagtcgtct gcttttttga tctcaggttt
     5281 ttttcaagag cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct
     5341 gttagtcctt tgaagattgc agttagttct tgacgtatag gttttataac tcgtttatct
     5401 atatcaggtt gcttataact tttaggaata tctaataatt caagaaagtc ttctttggag
     5461 aaatatgcga aacctttggt tctgtattgc tttaacagac gaaacatagt ctttgcatac
     5521 ccgtcctttt ccgtatttct ttttgattgt tagtcctttg aagattgcag ttagttcttg
     5581 acgtataggt tttataactc gtttatctat atcaggttgc ttataacttt taggaatatc
     5641 taataattca agaaagtctt ctttggagaa atatgcgaaa cctttggttc tgtattgtct
     5701 gtattgcttt aacagacgaa acatagtctt tgcatagcca ctttgtagct cgttgaattg
     5761 ttgtaaagaa tagcgtaccc attcgtatat ctacgtaagg attttctacg tccccgttga
     5821 ttttaaattc tgtaaacatt acaaacatat cgtaagaaag cccagactca cttctacgac
     5881 caaagcgtaa agataagatt ttttcataag ttgcttttaa atcttctaca aatcgtacat
     5941 ttgcagttgc tttgtagtta cttaaatctt ttagttggtc aaacgtaaaa cgaactgtct
     6001 tatccccttt ttcacgcatt ctagacacga tcgaactgtc ttatcccctt tttcacgcat
     6061 tctagacacg atggaaaaaa acaaatt
//
