LOCUS       AP026591                5995 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium JHP36 plasmid pJHP36_4 DNA, complete sequence.
ACCESSION   AP026591
VERSION     AP026591.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466805
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5995)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JHP36"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJHP36_4"
                     /geo_loc_name="Japan"
                     /collection_date="2002"
     gene            complement(1136..1306)
                     /locus_tag="EfmJHP36_35350"
     CDS             complement(1136..1306)
                     /locus_tag="EfmJHP36_35350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59056.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(1322..1858)
                     /locus_tag="EfmJHP36_35360"
     CDS             complement(1322..1858)
                     /locus_tag="EfmJHP36_35360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDP59057.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(1901..2824)
                     /gene="rep"
                     /locus_tag="EfmJHP36_35370"
     CDS             complement(1901..2824)
                     /gene="rep"
                     /locus_tag="EfmJHP36_35370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP59058.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKKGKKFII"
                     /name="rep"
     gene            complement(3694..4383)
                     /locus_tag="EfmJHP36_35380"
     CDS             complement(3694..4383)
                     /locus_tag="EfmJHP36_35380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59059.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(4623..5537)
                     /gene="mobA"
                     /locus_tag="EfmJHP36_35390"
     CDS             complement(4623..5537)
                     /gene="mobA"
                     /locus_tag="EfmJHP36_35390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDP59060.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="mobA"
     gene            complement(5519..5908)
                     /gene="mobC_2"
                     /locus_tag="EfmJHP36_35400"
     CDS             complement(5519..5908)
                     /gene="mobC_2"
                     /locus_tag="EfmJHP36_35400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDP59061.1"
                     /translation="MGLMSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC_2"
     Typing          complement(1880..2824)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.683"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(4627..5537)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agctcccctt gaatccccct caaaacccac gctccacaat acaaaaagtg gctagaaaat
       61 gctagccact ttttgtaaaa tttagtatga tttattttta gctatttaga gtttttattt
      121 ccgtaaatac ttctaaaaat tgctaagata aatgctatag gaattagata aagaatactt
      181 ttttgtaagc ttatattgaa aattagccta aatatgaaga acatagtgat taaaactatt
      241 attgaaataa ttacagctaa aatcttttta ttcatgagat agtttcactt tctgattatt
      301 ttattacata acgagcgtaa tcattgtatt gatatgatga tgttgtattg ctaaagtgag
      361 cattatatgt taattgtaca ccttttcctt gatttgcagc tcttacaaag acattcattg
      421 catcaacagc actactaaaa gcatttgtaa taacccatgt tccaactcca ccaataggaa
      481 taaaagatgc aatttgagca ggagtcgctt attataattt ttttcaaatc tctacacatt
      541 ttattagtaa cataatagtt cattcatttt atagcaagta aatccacgat atgattatat
      601 cgtgaatgaa cttgcagctt ccttatacct ctagcactaa aaggaaggaa acagatttaa
      661 ctacttgctc caacaatcat accgaacaaa aatagacaag taataatttt aataatttta
      721 tttttcattg ttacttcctc cttagatata aaatcctagg aaagcgcttt ctgtatatat
      781 ttagaataat cagaaaatta aatattgtca agagggttta ttttataaaa cgtgttattt
      841 taaaggttag tagacataat gtgtagtata ccaagtagcc cttgtatcaa ccgatacagg
      901 ggctcacttt gtgattttta attggtctag gtcaaaagct ctttttaagg cttgattttc
      961 tgcagttgct tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt
     1021 atttttataa aatatgataa aattttggta gcgctttata agcgcgttgc tttttcaaga
     1081 ataaactctc attatttttt taactggctg ctcgaaagag cagtcttttt ttttattatt
     1141 tatccgtttc ttttacaaac aattcttcgt taggactgtt gtaataattt ggattcaatt
     1201 tcaattcacc atgtttattc cctttattga tcactgacaa taacatcgga ctgaagccta
     1261 gaattcctag taaaatagaa ccgaatagaa gattttttct tgtcatgaat attccctcca
     1321 gttatttcca aagttgccac catttttgct tcttctttgt gtgtttttct atcgattctg
     1381 tttcttcaga aagaatagta gttgattcat cttctggagt ttcttccgta taaactaatt
     1441 gtaactgttc ttgcagtttt tcgatctgtt gatttgcctg tagggtgagc tgttgttgct
     1501 ggtcgagtaa tttttgcata ttagaattat tttgctcttt aagggaaagt tgttctcgta
     1561 aaaattcatt ttcttcacgc aacttatcta gcaactcgtt tacaaccacg tttttgcttg
     1621 atatataaat gtttttatcg ttgtcttttt catactcatg caacctatct agtaaccttt
     1681 ggttacctaa gttgctagca agttgcaata ctccagaacg caatacaacc ttataaccac
     1741 gctcattttt agctatcatg gaagaatcta atttttggat atgtttcgat attgcttgcc
     1801 tactcatctt aaaaagatct gacaattcct ttattgtaaa ggtttcttgt ggttgcatac
     1861 tggttgcctc ctaggttgct actggaataa attatctaaa tcagataata aacttttttc
     1921 cttttttttc cgaaagtgcg gactctgttt gtttttcaag agcttttttc tctttttcta
     1981 gttgtttgat ttgttggagg agttcaagtt ctcgtttctt ctgtttgatt ttttcgttaa
     2041 ttgttgtagc gggttcttcg tattcttttt gtttatcagc taaagccaat gctttatttt
     2101 ggtcttcttt ggttggtgca tgtttgttaa agtcgtctgc ttttttgatc tcaggtttga
     2161 acgtaaattg atagccaata acaggttttc cccgtccttt tccgtatttc tttttgattg
     2221 ttagtccttt gaagattgca gttagttctt gacgtatagg ttttataact cgtttatcta
     2281 tatcaggttg cttataactt ttaggaatat ctaataattc aagaaagtct tctttggaga
     2341 aatatgcgaa acctttggtt ctgtattgct ttaacagacg aaacatagtc tttgcatagc
     2401 cactttgtag ctcgttgaat tgttgtaaag aatagcgtac ccactcgtct aaattattta
     2461 acaaaggcag tgctttttcg taaattcgta tatctacgta aggattttct acgtccccgt
     2521 tgattttaaa ttctgtaaac attacaaaca tatcgtaaga aagcccagac tcacttctac
     2581 gaccaaagcg taaagataag attttttcat aagttgcttt taaatcttct acaaatcgta
     2641 catttgcagt tgctttgtag ttacttaaat cttttagttg gtcaaacgta aaacgaactg
     2701 tcttatcccc tttttcacgc attctagaca cgatggaaaa aaacaaattc atttcaaccg
     2761 gtgtaaattt tcttaaagga attgtattta attcatggtg gtattttaca atctcattag
     2821 ccatataatc acctcaatcc aattatgaat gacatttttt aaatgtcaag acattcatat
     2881 ttgacgttca aacagacaaa atgtccttca taaacagaca aaatagagtc cttcataaac
     2941 agacaaaatg tccttcataa acacacaaaa tgtccttcat aaatagacaa aatgtccttc
     3001 ataacattct gtatccattg gtagagtaag gacacagctt acggtaaaga ggtttaaaaa
     3061 gaggtttaaa aagagatata aaaagagggg ggctaatttt cccgcctaaa aaccacttct
     3121 tttttcttta aacaggttcg cgcatttttc caaaactgcc aatccaaaac cttgcttcct
     3181 acggtcgcca aaaaggtgtg atcgctacgc tctcacattg tactgcgcag tcgcctaaag
     3241 gctcctttgc taaaaacatg ttggtaaaga ggcctttgag cggttgtgaa tgcttatcta
     3301 agccgtttta agacagtctt ggataaaaat ccttatctag ctttaaagcg ccttacaggc
     3361 ggtcagggca agccctaaca accctaaaat aagcgatact taaaaacacc acacaagttt
     3421 aattttttaa gatataataa cttgtcgcct atccgacagt cttgcgactg gaaggaggtg
     3481 tatctaatgt ttgaattgtt catttgcccc cttcttatag aagtttgtgt ctctttattc
     3541 agttattggt tgaataaccg aaacaagaag taaaatcaat aaaggcgaca tcaacctgaa
     3601 aaaaagcccc agagaatccg acctctctgg ggctttcgtg tatctaatgt ttgatcattt
     3661 gcatgtttag tttaacatga ttgccgatca aattcaattt tgctgttgct gatctaacca
     3721 ttccttgaca ctttctagat aatttaatgc cgtttctttt ttcgttggac tttgtatgtt
     3781 agttgcttgt tgaaacaggt tttcgtagcg ttcatatgtg acagattgtt gtttttcttt
     3841 tttttcttct tcactggcaa accaagggtt tgtgtctcca aatagatcac tacaggcttc
     3901 ttctatcttt gtatctttta catattgcac gtcctttttt acttgttcat atttttcttg
     3961 gtattctttt tgcggttctt ctggcaaatt ccgataaagt cgagtgagtg tttccatatt
     4021 tttatccgtc aaacgctttt cttgcttcac aaaaatatca ttcatgtcct cataacaagc
     4081 ggtaaatttt tctatttttt gtgctaaggc ttgctctttt ttctttttat tacgtgctga
     4141 ttcatacttt tgagtgacat ttcctaataa aatacttcta gaaacgcgct tattgccctc
     4201 taaaacgcct aataagccgc ttgaaacgat tatcaaggaa aatatacagc caagcaagaa
     4261 aaatcgctta aatcgaattt tagagccttt taatgaatat cttgtttgga aaattttata
     4321 gatgagccaa atacttcctg aaatactaac aaaaatgagt agatagggta aataaaatgc
     4381 cattttagcc tccccatact aaccaaaatg cgaacagtgt caggactaag caacataagg
     4441 gggcaagaaa atccattgat cgttgaatga acgccttttt ttgttcttta tttcgctgat
     4501 ctaatagctc ttgatggagt tttttaactt gtaaatcaat cgtagtttgt atcctttctt
     4561 gtgtgtaggg agcaagaaaa gcacgttgtt tctttatttc gttttctatg tcttttaacg
     4621 ttttagagcg ataattcttg ttggtggtct gttcctcttt caacgtgttg ttcatggttt
     4681 cttcgagccg gtctaatttc cggttgttca ggcgtaagag ttcgttgttc tcttcgtact
     4741 tcttctgcag tttcgcgaat gctagctcga gctcttcgta gctcgttggt tcgctgttgg
     4801 ttataggttc gttctcgtct gtccagctca tgtaaaatcg tctccttttc gtagtccttt
     4861 cctagtctgt tggatcgtac ttttttcttt ttgtggatca attcgtaagt gaaatttttc
     4921 ccgcgattga agcaatgaat gccgaatgtt ttgagggaat cacaaaattg gtcataggtt
     4981 ctggttttct tgtttttcat ggtttgatca atcgcaaacc gaatttcatc tttccaaaca
     5041 ggttcatttt tttcttttat tttgatttca gccatggttc gtttttcttc tttttttcct
     5101 cgttcagtga tacttaaacc atgagttaaa caaatttcgt cgttgatatc gtaacactta
     5161 tctaggaatg cttgaccatg tgcttgaaac ttttttcctg tttctaaatt gaccgaatta
     5221 atgacaatgt gattgtggat atgctcttta tctgtgtgcg tgtaaatcgc gatttggtat
     5281 tctggcgcga ttttttcagc gtattctttc cctagttggt tggctttttc agcggtaatt
     5341 tctcctggtc gaaaggactg aatcaataaa tgtgcttgta cacgatcgtc ttttccataa
     5401 agcatgcgta tttgtttcat ttcacttttg gcataatcaa tgtcacagtt tattcctgtt
     5461 ttgacggttg ctcgtggttc tgcgtaattg attgctcggc tacatgaagt ggttcgactt
     5521 actttaactg ttgccatatc ttttgtagct ccttttgcat gatttttagg ttttcggata
     5581 aacgttgagc gtcaccagga gcaacgtcat gttggtgtgt attgcaccac ttagcaattt
     5641 gattgatatt ccctccagct ttggctaaat cgcgcgcgat tgcctgggca atgtctggcg
     5701 ccactttcgg tgtcaccaga cgcgcgtttt gtgccttggc tttcacgaaa gccggcacac
     5761 tcatttgcaa agcttcagct gactgcttca gctttgcaaa ttcggattcg ctcactcgaa
     5821 aactgatttg ttttggctcg gatcgatagg tatatttttt agaaggatcg ctagccaaat
     5881 tttgattttg ttcgctcata agtcccaccc tttcagtacg tcctacggac gtatcgttcg
     5941 caataccatt atacctaaaa aggtacaatg agtaaagctc actaaaaggg tttgg
//
