LOCUS       AP026590                6809 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium JHP36 plasmid pJHP36_3 DNA, complete sequence.
ACCESSION   AP026590
VERSION     AP026590.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466805
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6809)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JHP36"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJHP36_3"
                     /geo_loc_name="Japan"
                     /collection_date="2002"
     gene            complement(23..352)
                     /locus_tag="EfmJHP36_35220"
     CDS             complement(23..352)
                     /locus_tag="EfmJHP36_35220"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59043.1"
                     /translation="MSWTDENEPITNSEPTSYEELELAFAKLQKKYEENNELLRLNNRK
                     LDRLEETMNNTLKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQVKN
                     SIKSY"
                     /name="hypothetical protein"
     gene            complement(804..1052)
                     /locus_tag="EfmJHP36_35230"
     CDS             complement(804..1052)
                     /locus_tag="EfmJHP36_35230"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     806,563,710,566"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59044.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKK"
                     /name="hypothetical protein"
     gene            complement(1034..1423)
                     /gene="mobC_1"
                     /locus_tag="EfmJHP36_35240"
     CDS             complement(1034..1423)
                     /gene="mobC_1"
                     /locus_tag="EfmJHP36_35240"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDP59045.1"
                     /translation="MGLMSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     GDAQRLSENLKIMQKELQKIWQQLR"
                     /name="mobC_1"
     gene            complement(1822..2076)
                     /locus_tag="EfmJHP36_35250"
     CDS             complement(1822..2076)
                     /locus_tag="EfmJHP36_35250"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59046.1"
                     /translation="MFKKQEIQKTDLIPFLGTWQTKDHSERLVITNLNTSKNIEQYTIL
                     VKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNKNN"
                     /name="hypothetical protein"
     gene            complement(2129..2623)
                     /locus_tag="EfmJHP36_35260"
     CDS             complement(2129..2623)
                     /locus_tag="EfmJHP36_35260"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59047.1"
                     /translation="MINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYP
                     NLNNSQIESKSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGN
                     YSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITN
                     W"
                     /name="hypothetical protein"
     gene            complement(2893..3276)
                     /locus_tag="EfmJHP36_35270"
     CDS             complement(2893..3276)
                     /locus_tag="EfmJHP36_35270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59048.1"
                     /translation="MKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQPIEEQ
                     AINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNT
                     IKYIYTVKKKIQMENIQEVFHNF"
                     /name="hypothetical protein"
     gene            complement(3676..3846)
                     /locus_tag="EfmJHP36_35280"
     CDS             complement(3676..3846)
                     /locus_tag="EfmJHP36_35280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59049.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(3862..4122)
                     /locus_tag="EfmJHP36_35290"
     CDS             complement(3862..4122)
                     /locus_tag="EfmJHP36_35290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     4204,4053,4126; possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59050.1"
                     /translation="MNFYENNFPLKSKIILICKNYSTSNNNQQQQLTLQANQQIEKLQE
                     QLQLVYTEETPEDESTTILSEETESIEKHTKKKQKWWQLWK"
                     /name="hypothetical protein"
     gene            complement(4208..4411)
                     /locus_tag="EfmJHP36_35300"
     CDS             complement(4208..4411)
                     /locus_tag="EfmJHP36_35300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     4204,4053,4126; possible pseudo due to internal stop codon"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59051.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDKGY"
                     /name="hypothetical protein"
     gene            complement(4585..5175)
                     /locus_tag="EfmJHP36_35310"
     CDS             complement(4585..5175)
                     /locus_tag="EfmJHP36_35310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around 4605"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59052.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEIF
                     TFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDKVN
                     ETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKGHWF
                     VKIEDFRALLAIPESYRMRDIDKQIFAQAKKNF"
                     /name="hypothetical protein"
     gene            complement(6024..6263)
                     /locus_tag="EfmJHP36_35320"
     CDS             complement(6024..6263)
                     /locus_tag="EfmJHP36_35320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     6450,6267"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59053.1"
                     /translation="MQYVKDTKIEEACSDLFGDTNPWFASEEEKKKKKQQSVTYERYEI
                     LLQQATNMQNPTKKETALNYLKSVKEWLDQQQQN"
                     /name="hypothetical protein"
     gene            complement(6251..6427)
                     /locus_tag="EfmJHP36_35330"
     CDS             complement(6251..6427)
                     /locus_tag="EfmJHP36_35330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     6450,6267"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59054.1"
                     /translation="MEEFTTCYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEV
                     QDNYERTKKTCNM"
                     /name="hypothetical protein"
     gene            complement(6436..6714)
                     /locus_tag="EfmJHP36_35340"
     CDS             complement(6436..6714)
                     /locus_tag="EfmJHP36_35340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /note="frameshifted, insertion/deletion at around
                     6450,6267"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP59055.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKSKH"
                     /name="hypothetical protein"
     Typing          complement(4440..5175)
                     /name="rep_cluster_1742"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.864"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(148..473)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.165"
                     /coverage="100.0"
                     /evalue="3.48e-125"
     Typing          complement(775..1052)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.362"
                     /coverage="100.0"
                     /evalue="4.08e-131"
ORIGIN
        1 ttctgttcct tatttcgctg gtctaatagc tcttgatgga gtttttaact tgtaaatcaa
       61 tcgtagtttg tatcctttct tgcgtataag gaacgagaaa agcacgttgt ttctttattt
      121 cgttttctat gtcttttaac gttttagagc gataattctt gttggtggtc tgttcctctt
      181 tcaacgtgtt gttcatggtt tcttcgagcc ggtctaattt ccggttgttc aggcgtaaga
      241 gttcgttgtt ctcttcgtac ttcttctgca gtttcgcgaa tgctagctcg agctcttcgt
      301 agctcgttgg ttcgctgttg gttataggtt cgttctcgtc tgtccagctc atgtaaaatc
      361 gtctcctttt cgtagtcctt tcctagtctg ttggatcgta cttttttctt tttgtggatc
      421 aattcgtaag tgaaattttt cccgcgattg aagcaatgaa tgccgaagtt ttgagggaat
      481 tacaaaattg gtcataggtt ctggtttttt gtttttcatt gtttgatcga tcgcaatccg
      541 aatttcgtct ttccaaacag gttcattttt cttttaattt tatttcagac atggttcgtt
      601 tttctggttt ctttcctcgc tcagtgattg gtaagttatg tgcaagacaa atctcgtcat
      661 ttaggtcata acatttgttt agaaatgctt gaccatgagc ttgaaatttt ttccagtctc
      721 cagattaacg gagttaatga caatgtgatt atgaatatgg tctttgtctg tatgtgtgta
      781 aatagctgtt tggtgatcgg gggctatttt tttgcatact ctgcccctag ttgattagct
      841 ttttcagcgg taatttctcc tggtcgaaaa gattgaatca ataagtgtgc ctgtacacgg
      901 tcgtcttttc catacagcat gcgaatttgt ttcatttcac ttttggcata atcgacgtca
      961 cagtttattc ctgttttaat ggttgctcgt ggttctgcgt aattaattgc acgactacaa
     1021 gaagtggttc ggcttacctt aattgttgcc atatcttttg tagctccttt tgcatgattt
     1081 ttaggttttc ggataaacgt tgagcgtcac caggagcaac gtcatgttgg tgtgtattgc
     1141 accacttagc aatttgattg atattccctc cagctttggc taaatcgcgc gcgattgcct
     1201 gggcaatgtc tggcgccact ttcggtgtca ccagacgcgc gttttgtgcc ttggctttca
     1261 cgaaagccgg cacactcatt tgcaaagctt cagctgactg cttcagcttt gcaaattcgg
     1321 attcgctcac tcgaaaactg atttgttttg gctcggatcg ataggtatat tttttagaag
     1381 gatcgctagc caaattttga ttttgttcgc tcataagtcc caccctttca gtacgtccta
     1441 cggacgtatc gttcgcaata ccattatacc taaaaaggta caatgagtaa agctcactaa
     1501 aagggtttgg caagctctat atttgctagc caaatacctc cgtatttggt gcaaataacc
     1561 gcttgtcaaa ggggcgcaag ctcccccttg aatccccctc aaaacccacg ctccacaata
     1621 caaaaagtgg ctagaaaatg ctagccactt tttaaagttt cgcttgtttc accttcggtg
     1681 aaattattgt tttgtaatat caaacaacta ttcatcaatc agctttaaaa aattcaattg
     1741 gtttagctgt cccttctgtc ccatcattcg ttgtcaaaat aaatacgagc ttttttggag
     1801 aatccaaact aaaagtgtaa tctaattgtt tttattagag cgcagtgtta gtaaattatt
     1861 tgttgctttg taaatgaaaa cttgttcatt tatatttaaa tttttatcaa tgatttgaat
     1921 tttatgattg ttctctttta ctaagatggt atattgttct atattttttg aagtatttaa
     1981 attagtgatt actagacgtt ctgaatgatc tttagtttgc caagttccta gaaaaggaat
     2041 cagatcagtc ttttgtattt cttgtttttt gaacaactag ttaagaaaag aactaacact
     2101 agacccataa ctaaagcaat gggtttattt accaatttgt aatggtattg tcccaagtaa
     2161 aaccacctcc aggtacagga attgtaactg gtcctgcatt tgcacttatt acagttgggt
     2221 agttatacca aggattccac acatagtaag tttgataacc accttgattg acccacccaa
     2281 ataatgcaag tgcatgacgg ctgttactat aatttccata accagcagtt cccatataaa
     2341 tggcatttcc ttttcttagt tgtgtttgga cctctgaaag gtttagtatt cgtgctactt
     2401 ttttaggata aacaccacag taatttgcat attttattat ttgatcttga ttaagacttt
     2461 ttgattcaat ttgactgttg tttaaattag gataggtata atgcataatg gctctagctg
     2521 aagtagcatg tacatctgtt ttgttacata agatagcagc ttctacgtaa gcagcacacc
     2581 acggaaaact tgtttgggtt tctgctatac gccaattaat cataataaag ttatctccgg
     2641 aggagacact ttttacacgt ttaaccgcag gaaaattttg ttttttagag aaatttttaa
     2701 ttttattttt tatatttacg gatataagat catttttttc tgtaaaggaa gaaacaccat
     2761 ttttttctgt acttaaaggt gatttccaaa gtagtttaat ttgtccatta tcggaataat
     2821 aaatgctatt atcttgaaaa aataaggttg tatctgttat attactatct actagatttt
     2881 gaagttcttt tgctaaaaat tgtgaaatac ttcctgaata ttctccattt gtattttttt
     2941 tttgacggta taaatatatt taatcgtatt atcagcgtct gaaaatactg gaaagaaaat
     3001 agaactattt gccgtatttt ctccatcgag cattttaaat ggcgaaccaa gagtatagtt
     3061 aatatcagtt tcggtgttat tttccattaa aagaattgaa gttatggagt gttcaaaatc
     3121 agttttcgca aaattaattg cttgttcttc aattggttgg gagttgatat tgaaattaga
     3181 attttctgaa gcataggtat ttgtaattcc tatactaaaa attccaatca tcaaggaaaa
     3241 gaggagacct agataaattt tttttttggg tttcaaaata atcacgcctt tgtgttaaat
     3301 ttaagtggta tacacctata attaaaatat acaatatgcg ataacaaatt tttgttatag
     3361 ttcataaaat attgttttgg gaacataatc gttatttata gcgttagtta acccaagtag
     3421 cccttgtatc aaccgataca ggggctcact ttgtgatttt taattggtct aggtcaaaag
     3481 ctctttttaa ggcttgattt tctgcagttg cttctttccg tttctttgtg aatttttacc
     3541 agtcattttt tactggattg ttatttttta taaaatatga taaaattttg gtagcgcttt
     3601 ataagcgcgt tgctttttca agaataaact ctcattattt ttttaactgg ctgctcgaaa
     3661 gagcagtttt ttttattatt tatccgtttc ttttacaaac aattcttcgt taggactgtt
     3721 gtaataattt ggattcaatt tcaattcacc atgtttattc cctttattga tcactgacaa
     3781 taacatcgga ctgaagccta gaattcctag taaaatagaa ccgaatagaa gattttttct
     3841 tgtcatgaat attccctcca gttatttcca aagttgccac catttttgct tcttctttgt
     3901 gtgtttttct atcgattctg tttcttcaga aagaatagta gttgattcat cttctggagt
     3961 ttcttccgta taaactaatt gtaactgttc ttgcagtttt tcgatctgtt gatttgcctg
     4021 tagggtgagc tgttgttgct ggttgttgtt gctggtcgag taatttttgc atattagaat
     4081 tattttgctc tttaagggaa agttgttctc gtaaaaattc attttcttag caactcgttt
     4141 acaaccacgt ttttgcttga tatataaatg tttttatcgt tgtctttttc atactcatgc
     4201 aacctatcta gtaacctttg tctagtaacc tttggttacc taagttgcta gcaagttgca
     4261 atactccaga acgtaataca accttataac cacgctcatt tttagctaat aaggacgggt
     4321 ctaatttatc aacatgttta gagattgctt gcctacttat ttcaaataat tcagataatt
     4381 cctttattgt aaaggtttct ttgggttgca tgataattgc ctcctaaatt gttaggtatt
     4441 atttctctaa ccaattatct aaggtaattg aaggtatatt ttcttcaaaa taaatttgca
     4501 gagatcctat cttatttcct ttgtaggctt taattttttt gatttcaaaa gttttaaaaa
     4561 tagcagggtg agttgaatta acgtttaaaa attctttttc gcttgggcaa aaatttgttt
     4621 atcaatatct ctcatgcgat aactttctgg tattgctaat agagctctaa aatcttctat
     4681 ttttacaaac cagtgacctt ttttagatct tctgtcttta tgagccataa gttggcgata
     4741 taactcttta acgtagctac tctttaagtt tgtcatattt tctaattcga aacgagtaaa
     4801 attagttccg attgaattaa gtatatattc aaattcttca gaaaccttta ttaataatgt
     4861 ttcattcact ttatcgattt taaatttttg aaacagattt aattgttcat atgttttttc
     4921 gtcttcataa ataaaagaca aagtaatgat tttttttgac atttttatta aatcattagc
     4981 aaaacggtcg ccacgtgcat ttatattgta atcaattaat tcttttattt gtttaaatgt
     5041 aaaaatttct tcttttgtac cttttctttt cattttagaa catattcccc aaaataaatc
     5101 catatctata ggggtaaatt ttcttaaagg gacagaattt aatccattat tatatcgaac
     5161 tatatcgtta ctcaataaaa atcacctcaa taaattatgt tatcatagac ataagaggat
     5221 gtaaaacaca atttatcccc catagtgtga caatttatcc cccatagtgt gacaatttat
     5281 cccccatagt gtgacaattt atcccccata gtgtgacaat ttatccccca ctacctatgt
     5341 caatacttac tctcccataa ggttgctaat ccctaacagt tattattaac agtatatcta
     5401 acagtatatc taacagtata tcttaagagc acattgaatt tcccgcctaa aaactcctac
     5461 tttttcctcc aaaaggttcg cgaatttttc taaactggaa aacttgctcc ctacggtcgc
     5521 taaaaggtgt gatcgctatg ctctcatatt atactgcgca gtcgcctccg gctcctttgc
     5581 taaaaccgtt gttcaaagag gaggtgtgag actctgaaag cttgtctaag ccgttctaag
     5641 acaattcttg attaatttcc cttaatctag tcgagaagcg ccttacaggc ggtcagggca
     5701 agccctaaca accctaaaac aagtgatact taaaaacacc acacaaattt aattttttaa
     5761 gatataataa cttgtcgcct atccaacagt cttgcgactg gaaggaggtg tatctaatgt
     5821 ttgaactgtt catttgcccc cttcttgtag gagtttgtgt ctctgtattc agttattggt
     5881 tgaataaccg aaacaagaag taaaatcaat aaaggcgaca tcaacctgaa aaaaagcccc
     5941 agagaatccg acctctctgg ggctttcgtg tatctaatgt ttgatcattt gcacattcag
     6001 tttaacatga ttttaaatca aattcaattt tgttgttgct gatctaacca ttccttgaca
     6061 ctttttagat agtttaaggc ggtttctttt tttgtcggat tttgcatgtt agttgcttgt
     6121 tgaagtaaga tttcatagcg ttcatatgtg acagattgtt gttttttttt ttttttttct
     6181 tcttcactgg caaaccaagg gtttgtgtct ccaaatagat cactacaggc ttcttctatc
     6241 tttgtatctt ttacatattg cacgtctttt tggttcgctc atagttgtct tgaacctctt
     6301 tttgctgttt ttctggcaag ttttgataaa gacgagtgag tttttccatg tttttatccg
     6361 tcaaacgttt ttcttgattc acaaaaatat cgttcatttc ctcataacaa gtggtaaact
     6421 cttccatttt ttgtgctaat gcttgctctt tttcttttta tcacgcgctg attcatactt
     6481 ttgagtgaca tttcctaata aaatacttct agaaacgcgc ttatttccct ctaaaacgcc
     6541 gaataagccg cttgaaacga ttatcaagga aaatatacag ccaagaaaga aaaatcgctt
     6601 aaatcgaatt ttagagcctt ttaatgaata tcttgtttga aaaattttat agatgagcca
     6661 aatacttcct gaaatactaa caaaaatgag tagatagggt aaataaaatg ccattttagc
     6721 ctccccatac taaccaaaat gcgaacagtg tcaggactaa gcaacataag ggggcaagaa
     6781 aatccaatga tcgttgaata agcaccttt
//
