LOCUS       AP026584                2930 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium JHP35 plasmid pJHP35_4 DNA, complete sequence.
ACCESSION   AP026584
VERSION     AP026584.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466804
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JHP35"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJHP35_4"
                     /geo_loc_name="Japan"
                     /collection_date="2002"
     gene            complement(345..761)
                     /locus_tag="EfmJHP35_34420"
     CDS             complement(345..761)
                     /locus_tag="EfmJHP35_34420"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390085.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP55518.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIERNPDINNYSEAIRFLCMSYE
                     PDTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGILIGTNSKVYQETKEKIEK
                     QLNPSKRNQPKNRQKETNQTFTSKNFDPTNFLKE"
                     /name="hypothetical protein"
     gene            complement(763..1044)
                     /locus_tag="EfmJHP35_34430"
     CDS             complement(763..1044)
                     /locus_tag="EfmJHP35_34430"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002390111.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP55519.1"
                     /translation="MDILVRKIDSKVIAKIDKRCQELTNKTGKKWSRNRYLKVLIENDF
                     DHALMHYKKDQFDLLLEKFIAIQEKNTETLNEYVRTNNQLIETLIGGR"
                     /name="hypothetical protein"
     gene            1844..2818
                     /locus_tag="EfmJHP35_34440"
     CDS             1844..2818
                     /locus_tag="EfmJHP35_34440"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP55520.1"
                     /translation="MSKRGTIINEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVGT
                     LRENIYYHTPHDVLILDFEQLMRLNEGNGYLKAVGNNGWQLLDQHEENIAYIEILKFQE
                     GKGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVVDPV
                     SFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKVVPKEIDTWWRLELQLRRG
                     KATDWHAMVHESLDSFASPHYLPEDVKVTDRMMITALITEHSYWGQIHRNSKYKYRELL
                     KQESQNDELTNHLRETFSESADDLKRELDTWLQGLDVTEVGAE"
                     /name="replication initiation protein"
     Typing          1869..2815
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="82.899"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 caattgaccg aaatttatgt ctttgggttt tggggtttta aaacgaaaaa aagggggcga
       61 ttttcgcccc ctttgctcta tgtgaaaaat ttgttatact tattttatcc ttaaacatta
      121 ccaatctttt tcttatttag ctgctcgtac gagcagcttt ttttacgatt atgaattcaa
      181 atcatgtatt tataattttt ttgtttttat ttttttttct gttatttctt ctgaaattcg
      241 tttttttcat tcaattggtt actatcttat tgtaaatgtt gctattaaga tggtgatcct
      301 acccaaaaaa taaaccagga atttaaatct cctggtttaa ttttttattc ttttagaaaa
      361 tttgttgggt cgaaattttt agaagtaaat gtttgattgg tttctttttg acgatttttt
      421 ggttgatttc gtttggaggg attaagttgt ttttcaattt tttctttcgt ttcttgatac
      481 actttagaat ttgtgccaat aagaatacct gtatctttta gatataaatc agcaaattct
      541 gcaatgattt cggacatgat ggaaatttct tttcccatag catttagttt ggcttggttg
      601 ctggtagtcg tagtattggt atcaggttca taactcatac ataaaaaacg aatggcttcc
      661 gagtaattgt tgatatctgg atttctttcg ataatggaat ctaaaacgtc cacgtgactg
      721 ggataaaggg taacgtgttt ttgaacagaa gtttttctca tgttatcttc ctccgataag
      781 tgtttcaatg agttggttgt ttgtacgaac atattcattc agcgtttcag tatttttttc
      841 ttgaatagcg ataaattttt cgagtaataa atcgaattga tctttcttgt aatgcataag
      901 tgcgtggtca aaatcgtttt cgattaggac ttttaagtag cgatttcgac tccatttttt
      961 gccagtttta tttgtgagtt cttggcaacg tttgtctatt tttgcaataa cctttgaatc
     1021 gatcttccga actaaaatgt ccatagaaaa actcctttcg catttgtgta taatttatgt
     1081 atatcatacg aatatagtat ttgtatagct gttttttgct aaaatactat acataagcta
     1141 gccacgatgg cgaaaaagtg atatcaaatt tttttctgag aaacgtgata ggcacagcct
     1201 atctacccac tttttttaga aagtgggtac acgtttcctt atgctcttta taaaatggtg
     1261 atgtgcgact tttttctgtt cgtaaaaata aaaaaagttt acgaaaatga aactaaactg
     1321 aacgattatg acgggcatgt aatcaagatg aaatgatttt ataagcgcgt attttgcgtt
     1381 ctaagacgtt ttagcgttca tttcgtttag ttatcggcac aatcgttaaa acacgtaaga
     1441 gaagcgagaa tggagcctgt aaggcgactg gtagcgaaac ggcgtgttag attgtgcaag
     1501 ccgagaactg aatgaaatga agagcggaag cgtgaaataa tttcggcagg aggaggctca
     1561 aggggttcgg ggaatgaaat tccctgaggt ctttttattt ttttaagagg agtgctataa
     1621 tcaaagaaaa cgagaaaaaa gagcaaaata aaagccgaac ggtgttggaa gcacccttcg
     1681 gctaattacg gaaactactc acctagttcc gtaactcaaa ttcattatac gaatattttt
     1741 aggtttcttc aagctagaga tttttggata tggattttta gagttacgtg aactctggaa
     1801 attctttcca aaaatctctt ttttctttga ggaggaacaa aatttgagta aacgtggcac
     1861 gatcataaat gagtgggaaa aacttaaccg catatctaac aggcggttaa atgcacaaac
     1921 tatgtgcacc cccttaaaaa tgcaatggtc aattgaccga attaccattg ttggtacatt
     1981 aagggaaaat atttattatc acactcccca tgatgtgttg attttagatt ttgaacagct
     2041 tatgcgtcta aacgaaggta acggatattt aaaagcggtt ggaaataatg gctggcaatt
     2101 actagaccaa catgaggaaa atatagctta tattgagatt cttaagtttc aagagggaaa
     2161 agggcggatt gactttaacc ccaataaaat tagccaattt ctggcgggct cgatgaagaa
     2221 ctttatccat gatttatttt tagaaccaca tttctcacgt gcagatattg cttgtgacat
     2281 tattgacgtg ccagatgatt tcattaccca atatcgggtg gttgatcctg tttctttcaa
     2341 accaatttac ggtcggtctg ggaaactgga aacagcatat tggggaagtc gtgctagtga
     2401 acgacagatt cggatgtata acaaaaaatt ggaacaagaa agaaaacgga aagttgttcc
     2461 gaaagaaatc gatacttggt ggcgactaga attgcaattg cgaaggggga aagcaactga
     2521 ttggcatgca atggttcatg aaagtttaga tagctttgca agtcctcact atttgccaga
     2581 agatgtgaaa gtaactgaca gaatgatgat tactgctctt ataacggaac atagttactg
     2641 ggggcaaatt catagaaatt caaaatataa atatcgtgaa ttgttaaagc aagaaagtca
     2701 aaatgatgag ttgacgaatc atttgcgaga aacattttcg gagtcggcag atgatttgaa
     2761 acgagaatta gatacatggc ttcaaggact tgatgtgacg gaagtaggtg ctgaatgagt
     2821 gattcgatag aaaaaatgac cctaaaaaaa cttgctgatg aattgggtgt atcgaaaaat
     2881 acggtgcgca atcaactggt caattcggga tttgaccttg ctcagtataa
//
