LOCUS       AP026583                6031 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium JHP35 plasmid pJHP35_3 DNA, complete sequence.
ACCESSION   AP026583
VERSION     AP026583.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466804
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6031)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JHP35"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJHP35_3"
                     /geo_loc_name="Japan"
                     /collection_date="2002"
     gene            complement(147..836)
                     /locus_tag="EfmJHP35_34350"
     CDS             complement(147..836)
                     /locus_tag="EfmJHP35_34350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289349.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP55511.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFLLG
                     CIFSLIIVSSGLLGVLEGNKRVSRSILLGNVTQKYESARNKKKKEQALAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEPQKEYQEKYEQVKKDVQYVKDTKIEEA
                     CSDLFGDTNPWFASEEEKKEKQQSVTYERYENLFQQATNIQSPTKKETALNYLESVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     gene            complement(1076..1990)
                     /gene="mobA"
                     /locus_tag="EfmJHP35_34360"
     CDS             complement(1076..1990)
                     /gene="mobA"
                     /locus_tag="EfmJHP35_34360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289347.1"
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="BDP55512.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGKEYAEKIAPEYQIAIYTHTDKEHIHNHI
                     VINSVNLETGKKFQAHGQAFLDKCYDINDEICLTHGLSITERGKKEEKRTMAEIKIKEK
                     NEPVWKDEIRFAIDQTMKNKKTRTYDQFCDSLKTFGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="mobA"
     gene            complement(1972..2361)
                     /gene="mobC"
                     /locus_tag="EfmJHP35_34370"
     CDS             complement(1972..2361)
                     /gene="mobC"
                     /locus_tag="EfmJHP35_34370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_014748751.1"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="BDP55513.1"
                     /translation="MGLMSEQNQNLASDPSKKYTYRSEPKQISFRVSESEFAKLKQSAE
                     ALQMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAP
                     GDAQRLSENLKIMQKELQKIWQQLK"
                     /name="mobC"
     gene            complement(2813..3247)
                     /locus_tag="EfmJHP35_34380"
     CDS             complement(2813..3247)
                     /locus_tag="EfmJHP35_34380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP55514.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRYN
                     HNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSAVD
                     AMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
                     /name="hypothetical protein"
     gene            complement(3654..3824)
                     /locus_tag="EfmJHP35_34390"
     CDS             complement(3654..3824)
                     /locus_tag="EfmJHP35_34390"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002292472.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP55515.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNSP
                     NEELFVKETDK"
                     /name="hypothetical protein"
     gene            complement(3840..4376)
                     /locus_tag="EfmJHP35_34400"
     CDS             complement(3840..4376)
                     /locus_tag="EfmJHP35_34400"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002289340.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="BDP55516.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKVV
                     LRSGVLQLASNLGNQRLLDRLHEYEKDNDKNIYISSKNVVVNELLDKLREENEFLREQL
                     SLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLVYTEETPEDESTTILSEETESI
                     EKHTKKKQKWWQLWK"
                     /name="DUF536 domain-containing protein"
     gene            complement(4397..5341)
                     /gene="rep"
                     /locus_tag="EfmJHP35_34410"
     CDS             complement(4397..5341)
                     /gene="rep"
                     /locus_tag="EfmJHP35_34410"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000145511.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP55517.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVRF
                     TFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKINGD
                     VENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFAYFS
                     KEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGYQFTF
                     KPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELLQQIKQ
                     LEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
                     /name="rep"
     Typing          complement(4398..5341)
                     /name="rep_cluster_1197"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1080..1990)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.684"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ggttgaataa ccgaaacaag aagtaaaatc aataaaggcg acatcaacct gaaaaaaagc
       61 cccagagaat ccgacctctc tggggctttc gtgtatctaa tgtttgatca tttgcatgtt
      121 tagtttaaca tgattgccga tcaaattcaa ttttgctgtt gctgatctaa ccattccttg
      181 acactttcta gataatttaa tgccgtttct tttttcgttg gactttgtat gttagttgct
      241 tgttgaaaca ggttttcgta gcgttcatat gtgacagatt gttgtttttc ttttttttct
      301 tcttcactgg caaaccaagg gtttgtgtct ccaaatagat cactacaggc ttcttctatc
      361 tttgtatctt ttacatattg cacgtccttt tttacttgtt catatttttc ttggtattct
      421 ttttgcggtt cttctggcaa attccgataa agtcgagtga gtgtttccat atttttatcc
      481 gtcaaacgct tttcttgctt cacaaaaata tcattcatgt cctcataaca agcggtaaat
      541 ttttctattt tttgtgctaa ggcttgctct tttttctttt tattacgtgc tgattcatac
      601 ttttgagtga catttcctaa taaaatactt ctagaaacgc gcttattgcc ctctaaaacg
      661 cctaataagc cgcttgaaac gattatcaag gaaaatatac agccaagcaa gaaaaatcgc
      721 ttaaatcgaa ttttagagcc ttttaatgaa tatcttgttt ggaaaatttt atagatgagc
      781 caaatacttc ctgaaatact aacaaaaatg agtagatagg gtaaataaaa tgccatttta
      841 gcctccccat actaaccaaa atgcgaacag tgtcaggact aagcaacata agggggcaag
      901 aaaatccatt gatcgttgaa tgaacgcctt tttttgttct ttatttcgct gatctaatag
      961 ctcttgatgg agttttttaa cttgtaaatc aatcgtagtt tgtatccttt cttgtgtgta
     1021 gggagcaaga aaagcacgtt gtttctttat ttcgttttct atgtctttta acgttttaga
     1081 gcgataattc ttgttggtgg tctgttcctc tttcaacgtg ttgttcatgg tttcttcgag
     1141 ccggtctaat ttccggttgt tcaggcgtaa gagttcgttg ttctcttcgt acttcttctg
     1201 cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgctgt tggttatagg
     1261 ttcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtagtcc tttcctagtc
     1321 tgttggatcg tacttttttc tttttgtgga tcaattcgta agtgaaattt ttcccgcgat
     1381 tgaagcaatg aatgccgaat gttttgaggg aatcacaaaa ttggtcatag gttctggttt
     1441 tcttgttttt catggtttga tcaatcgcaa accgaatttc atctttccaa acaggttcat
     1501 ttttttcttt tattttgatt tcagccatgg ttcgtttttc ttcttttttt cctcgttcag
     1561 tgatacttaa accatgagtt aaacaaattt cgtcgttgat atcgtaacac ttatctagga
     1621 atgcttgacc atgtgcttga aacttttttc ctgtttctaa attgaccgaa ttaatgacaa
     1681 tgtgattgtg gatatgctct ttatctgtgt gcgtgtaaat cgcgatttgg tattctggcg
     1741 cgattttttc agcgtattct ttccctagtt ggttggcttt ttcagcggta atttctcctg
     1801 gtcgaaagga ctgaatcaat aaatgtgctt gtacacgatc gtcttttcca taaagcatgc
     1861 gtatttgttt catttcactt ttggcataat caatgtcaca gtttattcct gttttgacgg
     1921 ttgctcgtgg ttctgcgtaa ttgattgctc ggctacatga agtggttcga cttactttaa
     1981 ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg ataaacgttg
     2041 agcgtcacca ggagcaacgt catgttggtg tgtattgcac cacttagcaa tttgattgat
     2101 attccctcca gctttggcta aatcgcgcgc gattgcctgg gcaatgtctg gcgccacttt
     2161 cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca cactcatttg
     2221 caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc gaaaactgat
     2281 ttgttttggc tcggatcgat aggtatattt tttagaagga tcgctagcca aattttgatt
     2341 ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt tcgcaatacc
     2401 attataccta aaaaggtaca atgagtaaag ctcactaaaa gggtttggca agctctatat
     2461 ttgctagcca aatacctccg tatttggtgc aaataaccgc ttgtcaaagg ggcgcaagct
     2521 cccccttgaa tccccctcaa aacccacgct ccacaataca aaaagtggct agaaaatgct
     2581 agccactttt tgtaaaattt agtatgattt atttttagct atttagagtt tttatttccg
     2641 taaatacttc taaaaattgc taagataaat gctataggaa ttagataaag aatacttttt
     2701 tgtaagctta tattgaaaat tagcctaaat atgaagaaca tagtgattaa aactattatt
     2761 gaaataatta cagctaaaat ctttttattc atgagatagt ttcactttct gattatttta
     2821 ttacataacg agcgtaatca ttgtattgat atgatgttgt attgctaaag tgagcattat
     2881 atgttaattg tacacctttt ccttgatttg cagctcttac aaagacattc attgcatcaa
     2941 cagcactact aaaagcattt gtaataaccc atgttccaac tccaccaata ggaataaaag
     3001 atgcaatttg agcaggagtc tttagcttat tataattttt tttcaaatct ctacacattt
     3061 tattagtaac ataatagttc attttatagc aagtaaatcc acgatgatta tgattatatc
     3121 gtgaacttgc agcttccact tttatacctc tagcactaaa aggaaacaga tttaacggag
     3181 tacttgctcc aacaatcata ccgaacaaaa atagacaagt aataatttta ataattttat
     3241 ttttcattgt tacttcctcc ttagatataa aatcctagga aagcgctttc tgtatatatt
     3301 tagaataatc agaaaattaa atattgtcaa gagggtttat tttataaaac gtgttatttt
     3361 aaaggttagt agacataatg tgtagtatac caagtagccc ttgtatcaac cgatacaggg
     3421 gctcactttg tgatttttaa ttggtctagg tcaaaagctc ttttttaagg cttgattttc
     3481 tgcagttgct tctttccgtt tctttgtgaa tttttaccag tcatttttta ctggattgtt
     3541 attttttata aaatatgata aaattttggt agcgctttat aagcgcgttg ctttttcaag
     3601 aataaactct cattattttt ttaactggct gctcgaaaga gcagtctttt ttattattta
     3661 tccgtttctt ttacaaacaa ttcttcgtta ggactgttgt aataatttgg attcaatttc
     3721 aattcaccat gtttattccc tttattgatc actgacaata acatcggact gaagcctaga
     3781 attcctagta aaatagaacc gaatagaaga ttttttcttg tcatgaatat tccctccagt
     3841 tatttccaaa gttgccacca tttttgcttc ttctttgtgt gtttttctat cgattctgtt
     3901 tcttcagaaa gaatagtagt tgattcatct tctggagttt cttccgtata aactaattgt
     3961 aactgttctt gcagtttttc gatctgttga tttgcctgta gggtgagctg ttgttgctgg
     4021 tcgagtaatt tttgcatatt agaattattt tgctctttaa gggaaagttg ttctcgtaaa
     4081 aattcatttt cttcacgcaa cttatctagc aactcgttta caaccacgtt tttgcttgat
     4141 atataaatgt ttttatcgtt gtctttttca tactcatgca acctatctag taacctttgg
     4201 ttacctaagt tgctagcaag ttgcaatact ccagaacgca atacaacctt ataaccacgc
     4261 tcatttttag ctatcatgga agaatctaat ttttggatat gtttcgatat tgcttgccta
     4321 ctcatcttaa aaagatctga caattccttt attgtaaagg tttcttgtgg ttgcatactg
     4381 gttgcctcct aggttgctac tggaataaat tatctaaatc agataataaa ctttttttct
     4441 ctttttccga aagtgcggac tctgtttgtt tttcaagagc ttttttctct ttttctagtt
     4501 gtttgatttg ttggaggagt tcaagttctc gtttcttctg tttgattttt tcgttaattg
     4561 ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa agccaatgct ttattttggt
     4621 cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt tttgatctca ggtttgaacg
     4681 taaattgata gccaataaca ggttttcccc gtccttttcc gtatttcttt ttgattgtta
     4741 gtcctttgaa gattgcagtt agttcttgac gtataggttt tataactcgt ttatctatat
     4801 caggttgctt ataactttta ggaatatcta ataattcaag aaagtcttct ttggagaaat
     4861 atgcgaaacc tttggttctg tattgcttta acagacgaaa catagtcttt gcatagccac
     4921 tttgtagctc gttgaattgt tgtaaagaat agcgtaccca ctcgtctaaa ttatttaaca
     4981 aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg attttctacg tccccgttga
     5041 ttttaaattc tgtaaacatt acaaacatat cgtaagaaag cccagactca cttctacgac
     5101 caaagcgtaa agataagatt ttttcataag ttgcttttaa atcttctaca aatcgtacat
     5161 ttgcagttgc tttgtagtta cttaaatctt ttagttggtc aaacgtaaaa cgaactgtct
     5221 tatccccttt ttcacgcatt ctagacacga tggaaaaaaa caaattcatt tcaaccggtg
     5281 taaattttct taaaggaatt gtatttaatt catggtggta ttttacaatc tcattagcca
     5341 tataatcacc tcaatccaat tatgaatgac attttttaaa tgtcaagaca ttcatatttg
     5401 acgttcaaac agacaaaatg tccttcataa acagacaaaa tgtccttcat aaacagacaa
     5461 aatgtccttc ataaacagac aaaatgtcct tcataacatt ctgtatccat tggtagagta
     5521 aggacacagc ttacggtaaa gaggtttaaa aagaggttta aaaagagata taaaaagagg
     5581 ggggctaatt ttcccgccta aaaaccactt cttttttctt taaacaggtt cgcgcatttt
     5641 tcaaaactgc caatccaaaa ccttgcttcc tacggtcgcc aaaaaggtgt gatcgctacg
     5701 ctctcacatt gtactgcgca gtcgcctaaa ggctcctttg ctaaaaacat gttggtaaag
     5761 aggcctttga gcggttgtga atgcttatct aagccgtttt aagacagtct tggataaaaa
     5821 tccttatcta gctttaaagc gccttacagg cggtcagggc aagccctaac aaccctaaaa
     5881 taagcgatac ttaaaaacac cacacaagtt taatttttta agatataata acttgtcgcc
     5941 tatccgacag tcttgcgact ggaaggaggt gtatctaatg tttgaattgt tcatttgccc
     6001 ccttcttata gaagtttgtg tctctttatt c
//
