LOCUS       AP026578                4376 bp    DNA     circular BCT 11-AUG-2022
DEFINITION  Enterococcus faecium JHP10 plasmid pJHP10_4 DNA, complete sequence.
ACCESSION   AP026578
VERSION     AP026578.1
DBLINK      BioProject: PRJDB13429
            BioSample: SAMD00466803
KEYWORDS    .
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1
  AUTHORS   Hashimoto,Y., Suzuki,M. and Tomita,H.
  TITLE     Analysis of enterococcal linear plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Hashimoto,Y. and Tomita,H.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2022) Contact:Yusuke Hashimoto Gunma University,
            Bacteriology Graduate School of Medicine; 3-39-22, Showa, Maebashi,
            Gunma 371-0804, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 2.1.1
            Genome Coverage       :: 50x
            Sequencing Technology :: Illumina miniseq; Nanopore MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="JHP10"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="pJHP10_4"
                     /geo_loc_name="Japan"
                     /collection_date="2000"
     gene            145..1035
                     /locus_tag="EfmJHP10_35070"
     CDS             145..1035
                     /locus_tag="EfmJHP10_35070"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011117535.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="BDP52071.1"
                     /translation="MYTPLKMQWSIDRITIVGKLKENIYYHTQNDVLILDFEQLMRVNE
                     GNGYLKAVGNNGWQLLDQYEENIAYIEILKWQDGKGRIDFNPNKIGHFLASSMKNFIHD
                     LFLEPHFSRADIACDIIDVPDEFITQYRVVDPVSFKPIYGRSGKLETAYWGSRASERQI
                     RLYNKKLEQETKRKIVPPEIETWWRLELQLRRGKATDWHAMVHESLNSFASPHFLPLDV
                     KTNDKVMIFGLIANPEMWGFLERRMKYKLRDILKRESQNDELTNHLRETFSESADVLKK
                     ELDTWLQGLDVTEEE"
                     /name="replication initiation protein"
     gene            1135..1275
                     /locus_tag="EfmJHP10_35080"
     CDS             1135..1275
                     /locus_tag="EfmJHP10_35080"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP52072.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGEDN
                     D"
                     /name="hypothetical protein"
     gene            1268..1687
                     /locus_tag="EfmJHP10_35090"
     CDS             1268..1687
                     /locus_tag="EfmJHP10_35090"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP52073.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVIM
                     LDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELTEM
                     IRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
                     /name="hypothetical protein"
     gene            1696..2004
                     /locus_tag="EfmJHP10_35100"
     CDS             1696..2004
                     /locus_tag="EfmJHP10_35100"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP52074.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERMMDVLN
                     SVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM"
                     /name="hypothetical protein"
     gene            2008..2370
                     /locus_tag="EfmJHP10_35110"
     CDS             2008..2370
                     /locus_tag="EfmJHP10_35110"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP52075.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKRF
                     GFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKKSQ
                     MSESKFTSIFNDVNLR"
                     /name="hypothetical protein"
     gene            2592..3998
                     /locus_tag="EfmJHP10_35120"
     CDS             2592..3998
                     /locus_tag="EfmJHP10_35120"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP52076.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDRQKTNDWHKHIDFTKSDENI
                     YLEQTNLADKYEELFGEVVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIGIL
                     DDFQTNRADGSSTGISKQQAEQNRVIANKILVQYYKEFQERNPNLAVYNAVIHNDEISP
                     HLHLNIVPVAEGYKRGVQKQPSFNKALVQQGIHADKDNGRVLFNNFRNQEVDSIERLMN
                     DYGWKRDIVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYISRQREENERDK
                     ALMASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDFVDLYQR
                     ATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQVENKKLKE
                     ENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFYRQNVSQRK
                     LERSEDEIEW"
                     /name="hypothetical protein"
     Typing          151..1025
                     /name="rep_cluster_943"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="84.032"
                     /coverage="97.0"
                     /evalue="0.0"
     Typing          2593..3995
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.88"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 agagttacgt gaacttttga aaattctttc cagaagtctc tttttctttg aggaggaaca
       61 aaatttgagt aaaaatggca taatcgtata aatgagtggg aaaatttaac agccctatct
      121 aacaggcggt taaatgtaca aaatatgtac acccccttaa aaatgcagtg gtcaattgac
      181 cgaattacta tcgttggaaa actgaaggaa aatatttatt atcacactca aaatgatgtg
      241 ttgattttag attttgagca attaatgcgg gtgaatgaag gcaatggcta tttaaaagcg
      301 gttgggaata acggctggca attattagac caatacgaag aaaatattgc ttatattgag
      361 attttgaaat ggcaagatgg caaagggcgg attgatttta acccgaataa gattggtcat
      421 tttttagcta gttcaatgaa aaatttcatt catgatttat ttctagaacc acatttctca
      481 cgtgcggata ttgcttgcga tattatcgat gtgcctgatg agtttattac acaatatcga
      541 gtggttgatc ctgtttcttt caagccaatt tatgggcggt ctgggaagct ggaaacagct
      601 tattggggca gtcgtgccag cgaacgccag attcggctat acaacaaaaa attggaacag
      661 gaaaccaagc gtaaaattgt gccaccagaa atagaaactt ggtggcgact ggaattgcag
      721 ttaaggcgtg ggaaagcaac agattggcat gcaatggttc atgaaagttt aaatagtttt
      781 gctagccctc attttctacc acttgatgta aaaacaaatg ataaggtgat gatttttgga
      841 ctgattgcta accctgaaat gtgggggttt ttagagcgta gaatgaaata caaattgaga
      901 gatattttga aaagagaaag tcaaaatgat gagttgacga atcatttacg agaaacattt
      961 tcggaatcag cagatgtttt gaaaaaagaa ttagacactt ggcttcaagg gttagatgta
     1021 acggaggagg aataagaatg gcagaaaaac aaaagcattt tggtaagaaa aattggttgg
     1081 tgaatttaac aggtgataac gttgtcggtg tgtttttttg gatagcaaaa aaaaatgtcc
     1141 ggtaagttac ttgatgtaac gcagtatgag ttgattttga aaaataataa aattgattcc
     1201 ccaatcatta tccctaagca tgctgttaaa tatgtttatg gggtagctga aagtaacgga
     1261 gaagataatg actgatttgc cagaaaaaat gaccataaaa aaccttgctg atgagttggg
     1321 tatatctaaa aatacagtgc gcaatcaact ggtcaatgca ggttatgacc ttgctcggta
     1381 taaagataat ggggttatca tgttagacaa ggaattgatt ggtgttgtgc ggtcaattta
     1441 tcggtcaaaa ggtggtcaat tgactggtga tttaccggtc aatgatgcag gcaatgattt
     1501 cccgttagaa aaagaaaatg aatttttgcg tgaacaatta gcggaaaaag ataggcatat
     1561 ttctgaatta acggaaatga ttcgtgacca acaaaaattg actttgcttc agcaacaaaa
     1621 aaacgatcag ttgttactag aaaatcaaga attaaaaaat cgaaaatggt ggcaattctg
     1681 gaagtgaggt gtgacatgga taaaattgat ttgagagaag aaacagtcga tagaattgaa
     1741 ttgatgtatg aacggttagc tgattatttg aatttgccgg atgaagataa ttatagtgaa
     1801 atgcttgaac gaatgatgga tgttctcaac tcggttatca atgctgatct agcaaaatct
     1861 tcagttttga ctaatgaatt tgttcataag catttagcgc aaacaactga tgaatcagaa
     1921 aaagaatttt ggttgaaatt aatgagtcat gttatgcagt taaaccaaat ttccgttatt
     1981 aattccaaag aaaaggagat gtagtttatg ggaatgactt tagcagatat taaagcgcaa
     2041 gaagaaaaat taaaaaaaga acaagcgaaa cttcgccaac agaaaaaacg cttgattgag
     2101 aaagaaaatg ctgcaatggg caaggctctt cgcaagcgtt ttgggtttga aacttttgct
     2161 gattttgagc ggtggctatc gtcgatttct gacgatcagc aacacgaaaa atcagtggat
     2221 aatgagtggc ttgctgatga aaaatcagcg ccaagcgaac gagattatcc cgatgaatct
     2281 gacggcttat tcggagtgat accgaataag aaaagtcaga tgagtgagag taaatttacg
     2341 tcaatattta atgacgtgaa tttacgataa gcgattattt tgcgtgaagc aaaataaagc
     2401 gtaaaaggag ttgagagcgt gacacgcttt cggctccttt tttcgttgtc acttgtgaca
     2461 gcgaataccc tcggagagca tttggaattg tgacagccat gtcacaatta ggaaaatgtt
     2521 actctttgac aaagagtaac agaaaggttt acaatctttt tattagcgac agcgcgaagg
     2581 agggttttaa cttggggttt agtatttcat ttaaaaaagg aacgaagaat acaagtattt
     2641 ctcataataa tcgtgagtta actgatcgcc agaaaaccaa tgattggcat aagcatattg
     2701 acttcacgaa atcggatgaa aatatttatt tagagcagac gaatctagca gataaatatg
     2761 aggaattgtt tggcgaagtg gtagaaaaat ataatgcgaa acaaaaaaga gccgatagaa
     2821 aaatcgagga ctatttagcg aaagtgcgca aggataaaaa gttggaacct caacgagaat
     2881 ttattgtgca aataggcatt ttagatgatt tccagacaaa tcgagcagat ggttcttcta
     2941 ctggcatatc gaaacaacaa gctgaacaaa atcgagtgat tgccaataaa attttggttc
     3001 agtattacaa ggagtttcaa gagagaaacc ctaatttggc tgtttataat gctgttattc
     3061 ataatgacga aataagtccg cacctgcact tgaatattgt gccggttgct gaaggttata
     3121 aacggggtgt tcaaaagcag ccgagcttta ataaagcact ggttcaacaa gggatacacg
     3181 ctgataaaga taatggtcga gtgcttttca ataattttag aaatcaagaa gttgactcta
     3241 tagaacgatt aatgaatgat tacggttgga aaagggatat tgtcggtacc aacaaaatta
     3301 aagatattca cgaatacaaa gaaatcatga gtgaaatttc tgaattgcgc caaacggctg
     3361 taaaagagcg tgagagcgtt tctgatgagt tgaggtatat ttctaggcaa agagaagaaa
     3421 acgagcgtga taaggctctg atggcttctg agagagcgaa gattgaagaa gagaagaaaa
     3481 atatgctgaa tgtaaaaaaa gagatggcgg tcattttcca aaaagatatg gttcctttag
     3541 aaagtttcaa gaggcttcct gatggttcgt atcatttatc tgggaatgat tttgttgatt
     3601 tgtatcaacg agctacgaga gcaacaaatg ccaagagaaa tgctgataac atgagtgagc
     3661 aatatttaca gatgcttgag caaaatacag aattaaatga gtatgtagct acattggaag
     3721 aagagcgtca tttgtctggt agaaaacaat tagcagattt acaagtggaa aataaaaagc
     3781 tgaaagaaga aaatcgctct ttacgccagc gtttagagcg attaaaaaac gcatttcaaa
     3841 aatcgatcac tcgtttgtct atccgttttg gtcttgcaga aaaggatatg gggctttctg
     3901 aggaattaga agtgttagag caggagaaaa ccttatttta tcgtcaaaac gtctcacagc
     3961 gtaaattaga gcgttctgaa gatgagatcg agtggtagtt acgatgtgca gtcaacggta
     4021 ccggaggcgg tttgtgccga ggataaggag ttgacggcta aaccactagc ctattaagct
     4081 gacatttgct aaaaaatggc cagtccattt tttggccaac ctagcgttag cgaagtttgg
     4141 gggcgccctt ttcaaagggt gcggttttat tttttggata tttgcatagc taaagagaag
     4201 tgctattatt gagttagcaa acaaatgaac tgaaaaaaga gtaaaaaaat agtcgagcaa
     4261 cgctaccaac gttctcgacc tttacggaaa ctaatcgcac tagctccgta acttcttaat
     4321 cattatacga atatttttag atttcttcaa gactagagat tttttggtaa tgaatt
//
