LOCUS       AP026536                6274 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM2805 plasmid pMTY2805_NA4 DNA, complete
            sequence.
ACCESSION   AP026536
VERSION     AP026536.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510839
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6274)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 11x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM2805"
                     /db_xref="taxon:562"
                     /plasmid="pMTY2805_NA4"
                     /geo_loc_name="Japan"
                     /collection_date="2006"
     gene            complement(315..752)
                     /locus_tag="TUM2805_47530"
     CDS             complement(315..752)
                     /locus_tag="TUM2805_47530"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27465.1"
                     /translation="MPKRGKSIRSSSRNEETKPSKSTAKTTDRFISISFKHFHNVDDVG
                     QSINTWKDDGSLPDLIEKLVYLTANGITKVISDGVFTNYTRFPESAVNDFRCPDEISSD
                     EDWGVIKNIGGQKRRVAGFLRDNVFYIVFFDRDHRFWKSQK"
                     /name="hypothetical protein"
     gene            complement(757..2055)
                     /locus_tag="TUM2805_47540"
     CDS             complement(757..2055)
                     /locus_tag="TUM2805_47540"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002379013.1"
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="BDP27466.1"
                     /translation="MMVNFSLSNAFSFMEAQNMSMLAVSSSKDDINLANVVSVRDERIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVVPFLLDEAGPTKPSEME
                     MTFIADGIKYRYGLSLFQGKIKEEWLYYTPKTRETILFERDGQLIEINKAAFSESNLFI
                     KDGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFSNVVFLSGSNEGMFTGITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPEFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTANGEISVPIDFESEGTKKIVYLLGPIYNAMINNNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGFSQVIAAVQDVNLMNTDCFRRDQIWFVDKDVKSGSSHLYSLVEYKEKQRS
                     LKPSYGNDYLSGAFDAIPLFDNLNEFEKLMPEG"
                     /name="abortive infection protein"
     gene            2754..2999
                     /locus_tag="TUM2805_47550"
     CDS             2754..2999
                     /locus_tag="TUM2805_47550"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27467.1"
                     /translation="MAFYVDSTAKFLTKTTEPEGSVWRYYYKPGNTCPASGIYRCTNCG
                     DEVTCNKDDPLPPQNHRQHNVPGDIRWELVVMTKTK"
                     /name="hypothetical protein"
     gene            complement(3529..3762)
                     /locus_tag="TUM2805_47560"
     CDS             complement(3529..3762)
                     /locus_tag="TUM2805_47560"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27468.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQENG
                     QLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
                     /name="hypothetical protein"
     gene            complement(3769..4329)
                     /locus_tag="TUM2805_47570"
     CDS             complement(3769..4329)
                     /locus_tag="TUM2805_47570"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27469.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLNG
                     QKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSLTA
                     LCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKFLVL
                     PSGVKGENNWTVEKKNAVRLVRE"
                     /name="hypothetical protein"
     gene            complement(5007..5354)
                     /locus_tag="TUM2805_47580"
     CDS             complement(5007..5354)
                     /locus_tag="TUM2805_47580"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27470.1"
                     /translation="MIPVKRERMLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPS
                     KTSGLPTLAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRD
                     EVRKQGERDDS"
                     /name="hypothetical protein"
     gene            5398..5589
                     /gene="rop_2"
                     /locus_tag="TUM2805_47590"
     CDS             5398..5589
                     /gene="rop_2"
                     /locus_tag="TUM2805_47590"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BDP27471.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDHA
                     DELYRSCLARFGDDGENL"
                     /name="rop_2"
     Typing          complement(5676..5762)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.43e-38"
     Typing          5773..6274
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.548"
                     /coverage="93.0"
                     /evalue="3.84e-164"
     Typing          complement(3423..5017)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.872"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(5390..5592)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="88.235"
                     /coverage="100.0"
                     /evalue="5.2e-12"
ORIGIN
        1 ccttttccgg taactatcaa cttgagtcca acccggaaag acacgacaaa acgccaatgg
       61 cagcagccac tggtaactga tttagaggag ttagtcttga agttatgcgc ctgttaaggc
      121 taaactgaaa gaacagattt ggttactgcg ctcctcataa gccagttacc ttggttcaca
      181 gagcgccaac tcttagaacc gtcgaaaaac cgcccgccaa ggcggttttt tcgtttacag
      241 agcaagagat tacgacgaga gcatcacaat ctcaagaaga tcttttctta acatacaata
      301 caactgtatc tcatctattt ttgactcttc cagaagcgat gatctctatc aaaaaacacg
      361 atatagaaca cattatctct cagaaatccg gcgactcttc ttttctgccc gccaatgttt
      421 tttataaccc cccaatcctc atcagaggat atctcatctg ggcatctaaa atcgtttaca
      481 gctgactcag gaaatcgagt gtagttagta aacacaccgt cagaaatcac tttagtgatt
      541 ccattcgccg ttagataaac caatttttca atgagatcag gtaggctacc atcatctttc
      601 catgtattta tggactgccc aacatcatcg acgttatgaa aatgcttaaa actaatagat
      661 ataaatctat cagtcgtttt cgctgtgctc tttgaaggtt ttgtttcctc attcctcgaa
      721 gagcttcgaa tgcttttgcc tctctttggc atataatcat ccctcaggca ttagcttttc
      781 aaattcatta agattatcaa ataacgggat tgcatcaaaa gcacctgata aataatcatt
      841 cccatatgat ggcttcaatg atctttgttt ctctttgtac tcaaccaaag aataaagatg
      901 gctactccct gactttacat ccttgtcaac aaaccaaatt tggtctcgtc taaagcaatc
      961 agtattcatt aaattaacat cctgaactgc agcgattact tggctaaacc cttcactttg
     1021 cgcatgaaat atcctaaata aaaacttaga taaaagggta tggaactttg aatcgaactc
     1081 atcaaccaat aataagttat tgttaatcat ggcgttataa ataggcccaa ggagatagac
     1141 tatctttttg gtcccctctg attcaaaatc aatgggaacc gaaatctccc cattagcagt
     1201 ttttttcacg actctaagag aaaatacttt ttgatttttt gcaaaattct gaatcgagct
     1261 gaataaattt tttaaattat catcatctgt ttttatgttg ccaaacggaa attcaggcgc
     1321 aacttcatca agataaatat cattaatctg aaaggctggt aatatttttt ttagccaagc
     1381 tttaaaatct tcagattcat ttataagctt catggtaatt ccagtaaaca ttccttcgtt
     1441 agaaccagaa agaaatacca cattactaaa ccaattcaca acacgctttg agtgctcccc
     1501 attaaatcca gcaacaacag aaacaaaggg aacatcttct cttgtcttct gaatgacccc
     1561 atcctttata aaaagatttg actcgctaaa tgctgcttta tttatttcaa taagttgacc
     1621 atctcgctca aagagaatag tctcccttgt ttttggcgtg tagtacaacc actcttcttt
     1681 tatcttccct tgaaataaag ataatccgta tctgtattta atcccatccg caataaaagt
     1741 catttccatt tcagatggtt tagtaggccc cgcctcatca agtaaaaaag ggactacatt
     1801 cttcgtaatg ctactttcga tagcttgcac agaatgcagg acaatatctt tcattaaaga
     1861 tattgtcttc atgagattag attttccgct agcgttagct ccgaaaatca aagcagactt
     1921 cagtatccgc tcatccctta ctgacactac attagccaag tttatatcgt ctttcgatga
     1981 cgacaccgcc aacatgctca tattttgagc ttccatgaag gaaaatgcgt ttgaaagact
     2041 aaagttaacc atcataagaa cacccctaac aacaccctta aactcaaaaa ttgcacaata
     2101 ttgacaaaat tctagattaa gaatagcata accccgtact aaaacaacaa aagggtccaa
     2161 tagacagcac attatgagta gataagttaa cgaccagctt aaaaagaggt gtatatgaag
     2221 ttttttctat accaaatggt cccatttgcc tcagtgatta gctcagcagt gctggctttt
     2281 cactcggtat ctgggtgggg atggttcctt tttgtcgcgt ttatgctcag tggcgctgac
     2341 actgtaaaaa aatacgtagg gagtgaaaca tgaatgagga gaagcaaaaa ttgttgaagt
     2401 aagtgggtta gcctgtacca ccagaactaa cccgtaacaa accatacgca aatgcggcgc
     2461 ttgagtacca cctcaagtgc tttgataaag ccagccacaa gatctgttgg ttgctacagt
     2521 acaattttga tagcaaaaat caacattcat gtggcgaaaa taggaaatgc aacatgcaga
     2581 cggacgagcc tcaggcatac atttttcgcc cttacataac ggtaaagggc aaacggataa
     2641 cccgtcctaa cgggggaatg tttaagatcc caattaatcg ggaaaagaaa aaacaataac
     2701 caccgaagca tgcaagcttc ggtccctaac ttagtttatg taaggaaagc aacatggcct
     2761 tttatgtaga tagcacagcg aaatttttaa caaaaacaac cgagcctgaa gggagtgtat
     2821 ggcggtatta ctataagccg ggaaatactt gtccggcttc cggcatttac cgctgcacga
     2881 actgtggtga tgaagtgacg tgcaataaag atgatccttt gccaccgcaa aatcacaggc
     2941 agcataatgt cccaggtgac atccgatggg aattggtagt gatgacaaag acgaagtaac
     3001 ttactaggcc gtctacggac ggctttttta tacctgccaa tcagctggat ttaccataac
     3061 atccgaattg tacgcaccgc tgaaatgcgt tcagcgcgat cacggctgct gacaggtaaa
     3121 aatagcaaca aaccacccga aaagctgccg cgatcgcacc tgataaattt taaccgtatg
     3181 catagctatt cagccatgtg aataacgctg gttttgcctg cgtaaacctc atgacactgt
     3241 tttttttcca tcttttcagt tgatgacata cgcagacatc gcgggatgag gctgaggaat
     3301 gagcgcgatc tggcaaagag gcaaaacaca gcaacaaaaa cgacacgcca gaatcgcgcc
     3361 cggatgcgtt tttaacgcgt tccggtacca tctggcaacc tcccggaaca actcaccgtc
     3421 atatccctat tgacgggcca cgccataccc gtgcttcccg ttcctgctct tcatgccagg
     3481 accgcgcacg ctcccgttcc aggcgtgcct gcctttcctg ttcatccctt atctgctgtt
     3541 cgtgataaat aaccgactca agtggtccac ctgctcgact aatctctgca cctgctgact
     3601 caagtcgtcg cactgttccc tcagttgccc gttctcctgt cgtgtcagct cgaacatatg
     3661 ctgcaaatcc gtgaaggcgc tctcccagtc tttcagccgc tgcgtatagt cctgctgcaa
     3721 ttgctctaag gcgttcagta agtgcatttc cagctctgtc atactcactt actccctgac
     3781 cagtcttact gcgttcttct tctccaccgt ccagttgttt tcccccttca ccccggacgg
     3841 caataccaga aatttcccgt tcctgccctc gtgatacgtc acaccccatg ttttttcccg
     3901 gagtttcgcc agcgtctctt cctggtccct gatagccagg atgttcgccg caatccggct
     3961 ttcctgccac tgaatcagcc cccatgacgc cagaaaaatc ccgcccgtga cgcaaagcgc
     4021 cgtcagcgac gggtacagta tccgcccttt gaccagcttc cagagcagct cttcctgccg
     4081 ccgggccagt tcgttctctg tggcgctgaa ctgcgcgttc acggcactgt tcagcgtctc
     4141 cagttgttct ttcaccgctg ctgtgtgtgc gctgatagcg tctctgattt tctgcccgtt
     4201 taagttcagt tccctgtcta cagacgcttc gagcttcctg aactcgctgt tcagtatgtt
     4261 ctctgtagag acggcacgct ctttcagttt cttctcgaag tctgtcccca tttgtaaaag
     4321 attgctcata cagcgcccct ttcagcctga gattacgccc gccctccggg tcggcgatac
     4381 tgatactgct cctggttgtc ctcacaacct caaaacctgc cgctgtaagc gcctcagtga
     4441 catcctgacg cgtttttagc gctccggcat ggtaaagagc ctccagtccc ctcgtaatcg
     4501 cttctgcggc ctcctgtttc gctttcggca gattattcgg ggtgacaagt gtccgcctgt
     4561 tctccggtgc gttcgggtcg tgcagcccgt aatggtgatt caccagtgtc tgccaggcat
     4621 tgattcgcgg acggtccgct cggtcgtaat agggctggag ccgttttccg ttcgccagct
     4681 ccatattcgg gatgacaaaa ttcagctcaa gacgcccctt gtcctggtgc tccacccaca
     4741 ggatgctgta ctgatttttt tcaagaccgg gcatcagtac ccgctcaaag ctctccatca
     4801 ccctttcacg ctctcccggt ggcagggtct gctctgcaaa agacagaacc cccgaggtgt
     4861 attttttcgc aaacggcgtg gcatcgatga gttcccgcac ctcttcggga gcaccccgca
     4921 gaactctcgc cccttcccgg ttacgctccc ggcccagcag gtaatcaacc ggaccactgc
     4981 caccgccttt tcccctggca tgaaacttaa ctatcatccc gctctccctg tttacggacc
     5041 tcatccctca gctcactcag ttcacgtccg atggccatca gtgcagccac cacatgaacc
     5101 cggtcatgcc ccgaccactg tccgctgttt atcttccggg ctatctgatt caggttattg
     5161 ccgaccgaag cgaactggcg caacagcggc ggtgccagtg tcggaagacc tgacgttttc
     5221 gatggcggtg cccccaggca gaccttacgc atccatgacg caagttgttt tccctcacaa
     5281 cgtgccagca gccgcgcatg ttcctcatcc gtgacccgta tcgtgagcat cctttcgcgt
     5341 ttcaccggta tcattaaaaa cctccgacag actccccaca catggagaaa cagaactgtg
     5401 actaaacagg aaaaaaccgc ccttaacatg gcccgcttca tcagaagcca gacgctgacc
     5461 ctgctggaaa aactgaatga actggacgcc gacgaccagg ctgacatctg cgaagcgctt
     5521 cacgaccacg ctgacgagct ttaccgcagc tgcctcgcac gcttcgggga tgatggtgaa
     5581 aacctctgac acatgcagct cccggagacg gtcacagctg gcctgtgagc ggatgccggg
     5641 agcagacaag cccgtcaggg cgcgtcagca ggttttagcg ggtgtcgggg cgcagccatg
     5701 acccagtcac gtagcgatag cggagtgtat actggcttaa ccatgcggca tcagtgcgga
     5761 ttgtatgaaa agtacgccat gccgggtgtg aaatgccgca cagatgcgta aggagaaaat
     5821 gcatgtcccg gcgcttttcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac
     5881 tgcggcgagc ggtactgact cacacaaaaa cggtaacaca gttatccaca gaatcagggg
     5941 ataaggccgg aaagaacatg tgagcaaaag accaggaaca ggaggaaggc cgcgccgctg
     6001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaaccgacg ctcaagtcag
     6061 aggtggcgaa acccgacagg acttaaagat accaggcgtt tccccctggt gactccctcg
     6121 tgcgctctcc tgttcctgcc attcggttta ccggtgtcac tccgctgtta tggccgcgtt
     6181 tgtctcattc cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt
     6241 atgcacgaac cccccgttca gtccgaccgc tgcg
//
