LOCUS       AP026535                6560 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM2805 plasmid pMTY2805_NA3 DNA, complete
            sequence.
ACCESSION   AP026535
VERSION     AP026535.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510839
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6560)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 11x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM2805"
                     /db_xref="taxon:562"
                     /plasmid="pMTY2805_NA3"
                     /geo_loc_name="Japan"
                     /collection_date="2006"
     gene            complement(123..356)
                     /locus_tag="TUM2805_47460"
     CDS             complement(123..356)
                     /locus_tag="TUM2805_47460"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27458.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="hypothetical protein"
     gene            complement(363..881)
                     /locus_tag="TUM2805_47470"
     CDS             complement(363..881)
                     /locus_tag="TUM2805_47470"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27459.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPCGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="hypothetical protein"
     gene            complement(1559..1804)
                     /locus_tag="TUM2805_47480"
     CDS             complement(1559..1804)
                     /locus_tag="TUM2805_47480"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27460.1"
                     /translation="MRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQ
                     WSSGDRVQVVAALMAIGDELRRLRLAVREQGVRDDS"
                     /name="hypothetical protein"
     gene            1947..2138
                     /gene="rop_1"
                     /locus_tag="TUM2805_47490"
     CDS             1947..2138
                     /gene="rop_1"
                     /locus_tag="TUM2805_47490"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BDP27461.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="rop_1"
     gene            complement(3892..5463)
                     /locus_tag="TUM2805_47500"
     CDS             complement(3892..5463)
                     /locus_tag="TUM2805_47500"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000381397.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="BDP27462.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEIN
                     RLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPSAL
                     RQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIETQR
                     PKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQGVEL
                     SRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTARLWVYV
                     RDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAA
                     CMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAAPLMQ
                     SLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAENALRGVAVG
                     RKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWK
                     VDLSSQ"
                     /name="IS66 family transposase"
     gene            complement(5483..5830)
                     /locus_tag="TUM2805_47510"
     CDS             complement(5483..5830)
                     /locus_tag="TUM2805_47510"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000624622.1"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="BDP27463.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLFI
                     FRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINWKH
                     PKRTERAGIRI"
                     /name="isocitrate lyase"
     gene            complement(5830..6222)
                     /locus_tag="TUM2805_47520"
     CDS             complement(5830..6222)
                     /locus_tag="TUM2805_47520"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP27464.1"
                     /translation="MVKKRPRRPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLF
                     NWRHQYRKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPA
                     GTLRLKGKLTPALLQTLIREIKGSSH"
                     /name="hypothetical protein"
     Typing          complement(2224..2311)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          2322..2878
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.571"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(20..1569)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.802"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccatttttca tggtttcacc atcccagcga agggccatcc agcgtgcgtt cctgtatttc
       61 cggctgacgc tcccgttcca gggataacac atgttcgcgc tcctgtatca accgttcctc
      121 tcttatctcc agttctcgct gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc
      181 tgcacctgct gactcaactg catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta
      241 agcgaaaaca tcttctgcaa ttccacgaag gcgctctccc attcgctcag ccgctgcata
      301 tagtcctgtt gcagctgctc taaggcgttc agcaaatgta tttccagctc tgtcactctg
      361 tgtcactcct tcagatgtac ccactctttc ccctgaaagg gaatcacctc cgctgatttc
      421 ccgcacggaa ggacaaggaa tttcctgttc ccgtcctgca caaactccac gccccatgtc
      481 ttcgcgttca gtttctgcaa tgtctcttcc tgcttcctga tatcttccag gttcgcctgt
      541 atcctcccgc caagatacca gagcgtcccg ccactcgcgg taaacaggag aaagactatc
      601 cccagtaaca tcatgcccgt attccctgcc agctttaaca tgtccttcct gtgctgcatc
      661 atcgcctctt tcaccccttc ccggtgtttt ttcagcgact cctctgtcga ggcggtgaac
      721 agggctatag cgtctttgat tttcgtctcg tttgatgtca cagcctcgct tacagattcg
      781 ccgagcctcc tgaactcgtt gttcagcatt ttctctgtag attcggctct ctctttcagc
      841 tttttctcga actccgcgcc cgtccgtaaa agattgctca taaaacgctc ctttcagtct
      901 gatattcctc ccgccgttcg ggtctgcaat gctgatactg cttcgcgtca ccctgaccac
      961 ttcaagtccc acctctgtga gcgcctgaat cacatcctga cggcctttta tctctccggc
     1021 acggtaaagc acatcaagcc cccgtgtaac gctctcagca agcgcctgtt tcttttccgg
     1081 caggttatca gggagagtca gcgtccggcg gttctccggg gcgttggggt catgcagccc
     1141 gtaatggtga ttcaccagcg tctgccaggc atcaattctc ggcctgtctg cacggtcgta
     1201 gtacggctgg aggcgttttc cggtctgtag ctccatgttc ggaatgacaa aattcagctc
     1261 aagccgtccc ttgtcctggt gctccaccca caggatgctg tactgattct tttcgagacc
     1321 gggcatcagt acacgctcaa aactcgccat cactttttca cgccctcccg gcggcagctc
     1381 cttctccgcg aacgacagaa cacctgacgt gtatttcttc gcaaatggcg tggcatcgat
     1441 gagttcccgg acttcttccg gatcaccctg aagcaccgtt gccccttccc ggttacgctc
     1501 ccttcccagc aggtaatcaa ccggaccact gccaccacct tttcccctgg catgaaactt
     1561 aactatcatc ccgcactccc tgttccctga cggccagacg cagccggcgc agctcatccc
     1621 cgatggccat cagtgcggcc accacctgaa cccggtcacc ggaagaccac tgcccgctgt
     1681 tcaccttacg ggctgtctga ttcaggttat ttccgatggc ggccagctga cgcagtaacg
     1741 gcggtgccag tgtcggcagc ttcccggaac gggcaaccgg ctcacccagg cagacccgcc
     1801 gcatccatac cgccagttgt ttaccctcac agcgttccag taaccgggca tgttcatcat
     1861 cagtaacccg tatcgtgagc atcctctcgc gtttcatcgg tatcattact cccgtgaaca
     1921 gaaatccccc ttacacggag gcatcagtga ctaaacagga aaaaaccgcc cttaacatgg
     1981 cccgctttat cagaagccag acattaacgc tgctggagaa gctcaacgaa ctggacgcag
     2041 atgaacaggc agatatctgt gaatcgcttc acgaccacgc cgatgagctt taccgcagct
     2101 gcctcgcacg tttcggggat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     2161 tcacagcttg tctgtgagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcag
     2221 gttttagcgg gtgtcggggc gcagctctga cccagtcacg tagcgatagc ggagtgtata
     2281 ctggcttaac catgcggcat cagtgcggat tgtatgaaaa gtgcaccatg ccgggtgtga
     2341 aatgccgcac agatgcgtaa ggagaaaatg tagcttccgg cgctcttccg cttcctcgct
     2401 cactgactcg ctacgctcgg tcgttcggct gcggcgagcg gtatcagcgc actcaaagac
     2461 ggtaacacag ttatccacag aatcagggga taaagccaaa agaaacatgt gagcaaaaaa
     2521 caagagcccg aaaaaggccg cgccggtggc gtttttccat aggctccgcc cccctgacga
     2581 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac ttaaagatac
     2641 caggcgtttc cccctggtgg ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     2701 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     2761 tggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     2821 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     2881 cacgacttaa cgccactggc agcagccatt ggtaactaga caaggtggat tgagatatta
     2941 agagttcttg aagtggtggc ctaactgcgg ctacactaga aggacagtat ttggtatctg
     3001 tgctccacca agccagttac cttcggaaga aagagttggt agctctagat tccggcaaac
     3061 aaaccaccgt tggtagcggt ggtttttttg tttgcaggca gcagattacg cgccgcaaaa
     3121 aaggatctca agaagatctt gaatcactct atgaaaattt tgttttatag ccgtatttgt
     3181 tgatgctata agccagagac cttctatgag aatagacata tcacatcaaa cacggcatac
     3241 cccgccaaac atgcttcctc gggaacaaaa ttgtgtagca atggcattat ctgcgtgctt
     3301 tagacaacaa ctaaaccctg tggtaaacag cttattaaaa gagagaataa tacactcccc
     3361 taaagaattg gaacatgaca acgcagtaat aagagccctg caaaaactac aaattcaaga
     3421 agtttgtaac agcacccttt gggagacagc caaacaacaa ctacttcaaa aatcggacgg
     3481 aagatatttt gccatcaatt ctaaacactt agcctttcca ggcccaggag aatctcacgc
     3541 cttctgttgc atcaaataca aaaatgcaat tggcattaac ggaaacaatg ccgaaaccca
     3601 atcgacccac taccaaccct atccctatga taaagtcagt atatggggac cattccctca
     3661 caatttaacg taatatctta attgtacgca ccgctaaaac gcgttcagcg cgatcacggc
     3721 agcagacagg taaaaatggc aacaaaccac ccgaaaaaat gccgcgatcg cgcctgataa
     3781 attttaaccg tatgaatacc tatgcaacca gatggtacag gccacattcc ccccacttaa
     3841 tccactgaag ctgccatttt tgtaagcgac tcgtcagaac cgtattgata tttactgaga
     3901 gctcagatca actttccagg gcaacagatc gcgtacccgg tttgccggcc agtcctggat
     3961 atgttcaatg acgtaacgca gccacttttc tggctccaca ttgttcagac ggcatgtgcc
     4021 gatcagcgag tacaacaccg ccgcatgttc accaccgctg tcggaacccg cgaacatcca
     4081 gtttttccgg cctacggcca ctccccgtaa ggcgttctct gcgatgttgt tgtcgatttc
     4141 cacccagcca ttactgcagt acacgttcag tgcatcccac tgtttcagca ggtatgcgaa
     4201 cgcttttgcc gtatctgagt gacgcgacag tgttttcatc tgttgctgta tccagtcata
     4261 cagtgactgc atcagtggcg cggctctggc ttttcttgcc gccagacgct gttctgctga
     4321 acagccccgg acctctgcct cgatggcata cagttcaccg atacgctgca gggcttccgt
     4381 ggtgatgtag gtgggcgctc ttgcatgcac atcgtggatt tttctccggg catgagccat
     4441 acacgcggct tccgttattc tgccggattc gtataacgcc cggtaaccac cgtaagcatc
     4501 ggcctgaagc acaccgctgt aaccggccag gtgattttgt ggatggatac ctttccggtc
     4561 cggactgtac gcgaaccaga ccgccggggg catctgtgaa ccggcgttac ggtcatcacg
     4621 gacgtagacc cacagccggg ctgtccgggt tttaccgctg cccggctcct ggaccgggac
     4681 ggggatatca tcagcatgga ctttaccggg catcagcaca tactggcgca ggacgtcata
     4741 cagcggctcc agcagttcag caacagcacc tgtccagcgc cccagtgtgg cacggctcag
     4801 ctccactccc tgacgacggt atatttctga ctggcggtat aacggcagat ggtctgcata
     4861 tttcccggtg acaacatggg ccagaagccc cgctccggca taactgcgtg caatgggttt
     4921 tgaaggtact ggtgcctgca cgatatggtc gcaccggcaa caggcctgtt tcggacgttg
     4981 tgtttcgata accttaaagg cgctgctgat aagctccagt tgctctgaca catcacatcc
     5041 cagagaactg agttcaccac cacaggcagg acagcattcc tcttccggcc ggataacccg
     5101 ggtttcacgg ggaagtgagg ccggtaacgg tttacgggct gaagactggc gcagggcgga
     5161 tggcagtacc gggtcatatt gctcacccag cgtttccgcc atttcttcct gaagtgcgct
     5221 gattcgctcc tgtgcttcct gtatctgccg ttcggttttt gcacgaagtt tttctgagct
     5281 tttaccgaac tgcatacgtt gcagtttcgc aaccagcgcc ttcagccggt tgatttcgga
     5341 agcataagcc gccacccgct gtgagagcag gcggttgtat tcagccatct ggcggatggt
     5401 gtcctgttgc gtctgcaaca gtgcccgcag gcgggcgttc tcatgagcaa gtgaggtgtc
     5461 catatcctca ctttacaacg ggttatatgc ggattccagc gcgttccgtt cgtttcgggt
     5521 gcttccagtt gataccttca agaagcatgg ataactgagc cggagtaagg tgcaccttgc
     5581 cgtcacgggt gactggccag acgaagcggc cccgctccag gcgtttggtg aagaggcaca
     5641 gtccgtcact gtcagcccac aacactttta tctggtcacc ccggcgtccg cggaagatga
     5701 acaggtgtcc ggagaacggg tcatccttca ggacgttctg aacttttgat gccaggccgt
     5761 taaagccatt tcgcatatcg gtgatacctg caaccagcca gatacgcgaa cctgcaggga
     5821 gagatatcat cagtggctgc tcccttttat ttcgcggata agtgtctgta ataacgccgg
     5881 cgtcagttta cctttaagcc tgagagttcc ggccggcaga accagctcac aacacagact
     5941 gtcggacggt gtatttatct gctctggttc ctgtgcgggg gccgggattt tattatccgg
     6001 ctccggcgtt aacgtcacgg gaagcagtgc cggcatattt tttccggaag gcagcaggcc
     6061 acctttccgg tattgatggc gccagttgaa gagcaggtta tcgttgattc cgttttcccg
     6121 ggcgatctgc gccacacagg ctccgggctg cagtgactgc tccactaagg cgattttaaa
     6181 ctcataaggg aagttgggcc gccggggacg tttttttacc atgggggctt cggatataac
     6241 ggtgctttca ggacgtacga ctggtaccgt ggaaaattgt ccgtaaaggc aggcatcaag
     6301 ttcctgctcc gacatgcctg cgggcaaagg ccacgaaagg ccagctctcc gaaagcgcac
     6361 gaacatacta caaactgttg attttggtac acccaggcga cgcccggcca caacccgggg
     6421 taaatgttct tcaaagtgaa gacgtaaagc ttcagtgatc caggtccggt gtttcatacg
     6481 atagtgtcca ttaaaaatga tggacattat ttttgtagag ccggaggaaa cagaccagac
     6541 ggtttaaatg agccggttac
//
