LOCUS       AP026517                6668 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM13735 plasmid pMTY13735_NA1 DNA, complete
            sequence.
ACCESSION   AP026517
VERSION     AP026517.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510805
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6668)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 133x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM13735"
                     /db_xref="taxon:562"
                     /plasmid="pMTY13735_NA1"
                     /geo_loc_name="Japan"
                     /collection_date="2013"
     gene            86..427
                     /locus_tag="TUM13735_47990"
     CDS             86..427
                     /locus_tag="TUM13735_47990"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP12400.1"
                     /translation="MSLRYYIKNILFGLYCTLIYIYLITKNSEGYYFLVSDKMLYAIVI
                     STILCPYSKYAIEHIAFNFIKKDFFERRKNLNNAPVAKLNLFMLYNLLCLVLAIPFGLL
                     GLFISIKNN"
                     /name="hypothetical protein"
     gene            complement(424..1992)
                     /locus_tag="TUM13735_48000"
     CDS             complement(424..1992)
                     /locus_tag="TUM13735_48000"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP12401.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKS
                     ESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRH
                     NASRTPSATELAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIERE
                     KAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSS
                     IHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAG
                     KIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNL
                     LNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDV
                     LNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTG
                     DWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTIN
                     EVLGI"
                     /name="hypothetical protein"
     gene            2252..2689
                     /locus_tag="TUM13735_48010"
     CDS             2252..2689
                     /locus_tag="TUM13735_48010"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP12402.1"
                     /translation="MGHCHFMAWHTLRDALKMTGRSRSQFYRDMRAGLVSYRTGRDGRR
                     EFETSELIRAYGELKQNETPERHSEGHAENPHDQQTERILRELNELKQCLTLMLEDKQA
                     QDMDRRRQEAEREQLQNEIAQLRQALELEKKRGFWSRLFGR"
                     /name="hypothetical protein"
     gene            2770..3189
                     /locus_tag="TUM13735_48020"
     CDS             2770..3189
                     /locus_tag="TUM13735_48020"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP12403.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRHYVYASDDGFD
                     PNFSTQKADTTRMMVPFFRQFWDMGAKDKATGKSRSDVQQRIQQFHSQEFLNSLRGTTQ
                     FAGTDYRSKDLTPKKSRLLADTISAVYLDGYEGRQ"
                     /name="hypothetical protein"
     gene            complement(3507..3761)
                     /locus_tag="TUM13735_48030"
     CDS             complement(3507..3761)
                     /locus_tag="TUM13735_48030"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP12404.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSEQTERLSQLYSENWR"
                     /name="hypothetical protein"
     gene            complement(3768..4286)
                     /locus_tag="TUM13735_48040"
     CDS             complement(3768..4286)
                     /locus_tag="TUM13735_48040"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP12405.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIRDAIALFTASTEESLEKHREGVKEAMMQHRRDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="hypothetical protein"
     gene            complement(4964..5209)
                     /locus_tag="TUM13735_48050"
     CDS             complement(4964..5209)
                     /locus_tag="TUM13735_48050"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDP12406.1"
                     /translation="MRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQ
                     WSSGDRVQVVAALMAIGDELRRLRLAVREQGARDDS"
                     /name="hypothetical protein"
     gene            5351..5542
                     /gene="rop"
                     /locus_tag="TUM13735_48060"
     CDS             5351..5542
                     /gene="rop"
                     /locus_tag="TUM13735_48060"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BDP12407.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="rop"
     Typing          complement(5628..5715)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          5726..6284
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.365"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3404..4974)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.809"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tttatcgcga atattcccac aaaaaatctt ttcctcataa ctcgatcctt ataaaatgaa
       61 aagaatatat ggcgaggttt aatttatgag cttaagatac tacataaaaa atattttatt
      121 tggcctgtac tgcacactta tatatatata ccttataaca aaaaacagcg aggggtatta
      181 tttccttgtg tcagataaga tgctatatgc aatagtgata agcactattc tatgtccata
      241 ttcaaaatat gctattgaac acatagcttt taacttcata aagaaagatt ttttcgaaag
      301 aagaaaaaac ctaaataacg cccccgtagc aaaattaaac ctatttatgc tatataatct
      361 actttgtttg gtcctagcaa tcccatttgg attgctagga ctttttatat caataaagaa
      421 taattaaatc cctaacacct catttatagt attaagttta ttcttatcaa tataggagca
      481 tagaattcct gtaacaatag caatacccca aatacctaat gtagttccag caagcaagct
      541 aaaaagtaaa gcaacaacat aactcacccc tgcatctgca gctttcttct ctaatgtaag
      601 aaatagtggc ttccagtcac ctgtatcctt aatttttagg atatcagata ccacatcata
      661 tccaaaagaa acatgccccg taatcttcaa gtacttggca aactgatcta aatgtttagc
      721 ccagtcatca tacttcaccg atgccaacgc attaaaaata gcatcacgat cggctttgct
      781 gaatttctta tttaaaacat ccttgtattt ttcaaaagca gcgagagctt cattcacatt
      841 gccgattttc ttacctttag acttatcagc aagttcctgt gccattttcg aatatttttc
      901 accatatttt tcagtcagcg tttgataaaa gctaactgtt gcatcaacag catccttaat
      961 ctgtgaatta aggagattat tctgtgcttt tttcaaattt tcttcagctt catgaacacg
     1021 agcgataccg gcattacgat tattactgac ctgagaaata gccttctgga tctgagttat
     1081 atcagcattt atccggttaa tacgtgtttc tgatgctgtt acctgttttt gtttttcttc
     1141 tctaatctta ccggccccaa cccgtcgtct ggttgcttca aaaaaaggac ggttctgaag
     1201 cggatcattg gctcttggtg atagtttttt gaccagctca tccagttctt tatatttagc
     1261 ggatgcctga gccagttcat ttcgttttcc agcgagcgtt ttcatttctg catcacgggc
     1321 atggatactg gagcttaaac gagaattgag agtcttaatc tctccatcca ttttcaccac
     1381 ttcagattgt gcagcagaaa gttttttttg ggcgatctca acagctttag cttcttcact
     1441 caatgcagcc agtcgtttct cttcagcttc agccagtttc aactggcgtt ctgtttcagc
     1501 cttctcccgt tcaatctctt tacgtcgttg ttctgcttcc tgaaaagcct tttctgctgc
     1561 ttccgcttct ttacgggctt tttcttctgc tttcgcaagg cgcaaacgct ctgcttccgc
     1621 ctgcatagct gcattattag catgagcaag ctctgttgct gaaggcgtac gtgaggcatt
     1681 gtgacgaaga gcctcattca cgatatcctt caggcgctga gtcagcgcat ccctgtttgc
     1741 ctttgctttc gcctgtgctt ccgctgcagc ttttgcccgg gcagcctgct ctgcctgtgt
     1801 tttctttaat tgagcagtag accatttagc agttgcatga atagctgcag aactttcact
     1861 tttactgcct ccttttccac ctccgccgcc agagccactc ccgtcaggag taccattcaa
     1921 aagagtaata attacctgtc ccttatcatc ataaggaaca ccatctttat agtacgctac
     1981 cgcggtttcc attataaaat cctctttgac ttttaaaaca ataagttaaa aataaatact
     2041 gtacatataa ccactggttt tatatacagc ataaaagcta cgccgctgca ttttccctgt
     2101 caagactgtg gacttccatt tttgtgaaaa cgatcaaaaa aacagtcttt cacaccacgc
     2161 gctattctcg cccgatgcca caaaaaccag cacaaacatt accgttctca gacctcatta
     2221 tgttttactg aaactatgag atgagacatc tatgggacac tgtcacttta tggcatggca
     2281 cacactccgg gacgcactaa aaatgacagg cagatcgcgt tcacagtttt accgtgatat
     2341 gcgcgcaggc cttgtcagtt accgtaccgg cagggacgga cgacgggagt ttgaaaccag
     2401 tgaactgatc cgggcatacg gcgaattaaa gcagaatgag acaccagaaa ggcacagtga
     2461 gggacatgca gaaaatccac atgatcagca gacagaacgc attctccggg agctgaatga
     2521 gctgaaacaa tgcctgacgc tgatgcttga ggataaacag gcacaggata tggatcgcag
     2581 acgccaggaa gcagaacggg aacagctaca aaatgagata gcccagctca ggcaggcact
     2641 ggaactggaa aagaaacggg gattctggtc caggttgttc ggtcgctgaa cgctgtcaga
     2701 gactgatgat aaaatagtct tcggataata actcaccgag aataaatact ttaaggtagg
     2761 gagacactca tgagacgtac cggaaacaaa ctttgtctta tcgccatgat aacagcaaca
     2821 gtagctctca cagcctgtac cccaaagggc agcgtggaac aacatacccg gcattacgta
     2881 tatgcttctg atgacggttt tgatcccaac ttttccaccc aaaaagccga cacaacacga
     2941 atgatggtgc ctttttttcg gcagttctgg gatatgggag ctaaagataa agcgacagga
     3001 aaatcacgga gtgatgtgca acaacgcatt cagcagtttc acagccagga atttttaaac
     3061 tcactccggg gcacaactca atttgcgggt actgattacc gcagcaaaga ccttaccccg
     3121 aaaaaatcca ggctgctggc tgacacgatt tctgcggttt atctcgatgg ctacgagggc
     3181 agacagtaag tggatttacc ataatccctt aattgtacgc accgctaaaa cgcgttcagc
     3241 gcgatcacgg cagcagacag gtaaaaatgg caacaaacca cccgaaaaac tgccgcgatc
     3301 gcgcctgata aattttaacc gtatgaatac ctatgcaacc agagggtaca ggccacatta
     3361 cccccactta atccactgaa gctgccattt ttcatggttt caccatccca gcgaagggcc
     3421 atccagcgtg cgttcctgta tttccggctg acgctcccgt tccagggata acacatgttc
     3481 gcgctcctgt atcagccgtt cctctcttat ctccagttct cgctgtataa ctggctcaag
     3541 cgttctgtct gctcgctcaa gcgttctgtc tgctcgctca agtgctgcac ctgctgactc
     3601 aactgcatga cccgctcgtt cagcatcgcg ttgtcccgtt gcgtaagcga aaacatcttc
     3661 tgcaattcca cgaaggcgct ctcccattcg ctcagccgct gcatatagtc ctgttgcagc
     3721 tgctctaagg cgttcagcaa atgtgtttcc agctctgtca ctctgtgtca ctccttcaga
     3781 tgtacccact ctttcccctg aaagggaatc acctccgctg atttcccgta cggaaggaca
     3841 aggaatttcc tgttcccgtc ctgcacaaac tccacgcccc atgtcttcgc gttcagtttc
     3901 tgcaatgtct cttcctgctt cctgatttct tccaggttcg cctgtatcct ccctccaaga
     3961 taccagagcg tcccgccact cgcggtaaac aggagaaaga ctatccccag taacatcatg
     4021 cccgtattcc ctgccagctt taacacgtcc ctcctgtgct gcatcatcgc ctctttcacc
     4081 ccttcccggt gtttttccag cgattcctct gtcgaggctg tgaacagggc tatagcgtct
     4141 ctgattttcg tctcgtttga tgtcacagcc tcgcttacag attcgccgag cctcctgaac
     4201 tcgttgttca gcattttctc tgtagattcg gctctctctt tcagcttttt ctcgaactcc
     4261 gcgcccgtct gcaaaagatt gctcataaaa tgctcctttc agcctgatat tcttcccgcc
     4321 gttcggatct gcaatgctga tactgcttcg cgtcaccctg accacttcca gccccgcctc
     4381 agtgagcgcc tgaatcacat cctgacggcc ttttatctct ccggcatggt aaagtgcatc
     4441 tatacctcgc gtgacgccct cagcaagcgc ctgtttcgtt tcaggcaggt tatcagggag
     4501 tgtcagcgtc ctgcggttct ccggggcgtt cgggtcatgc agcccgtaat ggtgattcac
     4561 cagcgtctgc caggcatcaa ttctaggcct gtctgcgcgg tcgtagtacg gctggaggcg
     4621 ttttccggtc tgtagctcca tgttcggaat gacaaaattc agctcaagcc gtcccttgtc
     4681 ctggtgctcc acccacagga tgctgtactg atttttttcg agaccgggca tcagtacacg
     4741 ctcaaagctc gccatcactt tttcacgtcc tcccggcggc agctccttct ccgcgaacga
     4801 cagaacaccg gacgtgtatt tcttcgcaaa tggcgtggca tcgatgagtt cccggacttc
     4861 ttccggatta ccctgaagca ccgttgcgcc ttcgcggtta cgctccctcc ccagcaggta
     4921 atcaaccgga ccactgccac caccttttcc cctggcatga aatttaacta tcatcccgcg
     4981 ccccctgttc cctgacggcc agacgcagcc ggcgcagctc atccccgatg gccatcagtg
     5041 cggccaccac ctgaacccgg tcaccggaag accactgccc gctgttcacc ttacgggctg
     5101 tctgattcag gttatttccg atggcggcca gctgacgcag taacggcggt gccagtgtcg
     5161 gcagttttcc ggaacgggca accggctccc ccaggcagac ccgccgcatc cataccgcca
     5221 gttgtttacc ctcacagcgt tcaagtaacc gggcatgttc atcatcagta acccgtattg
     5281 tgagcatcct ctcgcgtttc atcggtatca ttaccccatg aacagaaatc ccccttacac
     5341 ggaggcatca gtgactaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag
     5401 ccagacatta acgctgctgg agaagctcaa cgaactggac gcagatgaac aggccgatat
     5461 ttgtgaatcg cttcacgacc acgccgatga gctttaccgc agctgcctcg cacgtttcgg
     5521 ggatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgtg
     5581 agcggatgcc gggagctgac aagcccgtca gggcgcgtca gcaggtttta gcgggtgtcg
     5641 gggcgcagcc ctgacccagt cacgtagcga tagcggagtg tatactggct taaccatgcg
     5701 gcatcagtgc ggattgtatg aaaagtacgc catgccgggt gtgaaatgcc gcacagatgc
     5761 gtaaggagaa aatgcacgtc caggcgcttt tccgcttcct cgctcactga ctcgctacgc
     5821 tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac acagttatcc
     5881 acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagaa acaggaagaa
     5941 ggccgcgtag caggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatag
     6001 acgctcaagt cagaggtggc gaaacccgac aggactataa agacaccagg cgtttccccc
     6061 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6121 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgttggt atctcagttc
     6181 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6241 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaaggcacg ccttaacgcc
     6301 actggcagca gccactggta accggattag cagagcgatg atggcacaaa cggtgctaca
     6361 gagttcttga agtagtggcc cgactacggc tacactagaa ggacagtatt tggtatctgc
     6421 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6481 accaccgttg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6541 ggatctcaag aagatccttt gatcttttct actgaaccgc gatccccgtc agtttagaag
     6601 aggaggatgg tgcgatggtc cctccctgaa catcacgtat atagttagcc tgacatccaa
     6661 caaggagg
//
