LOCUS       AP026494                4867 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM9754 plasmid pMTY9754_NA4 DNA, complete
            sequence.
ACCESSION   AP026494
VERSION     AP026494.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510791
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 230x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM9754"
                     /isolation_source="animal"
                     /db_xref="taxon:562"
                     /plasmid="pMTY9754_NA4"
                     /geo_loc_name="Japan"
                     /collection_date="2010"
     gene            1567..1893
                     /locus_tag="TUM9754_50310"
     CDS             1567..1893
                     /locus_tag="TUM9754_50310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87552.1"
                     /translation="MEKRTKEIKIRLTEAEHQRLLERCDRKHLAEWLRAVGLGESRTAR
                     RRPLPTVDPTLLRQVSGIGNNLNQIARYLNQHGLPPQERVSLLAVLNSIDQHLAELLEQ
                     HRDR"
                     /name="hypothetical protein"
     gene            2571..3056
                     /locus_tag="TUM9754_50320"
     CDS             2571..3056
                     /locus_tag="TUM9754_50320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87553.1"
                     /translation="MSEILNLAREFESKSKQQAKTTATSVANAFEKHEKRITEALKLSS
                     GNIQTAIQEENDSQLKQIHRLVGMTWLYSLALSGILFAILIGVAWYLGTIVVERQNEIS
                     EQSQILQDLKSQTGAGVSIIHDSKNKSVYYLILPQGAKKIEEYKNAQHRQVIKYSAK"
                     /name="hypothetical protein"
     gene            3117..3350
                     /locus_tag="TUM9754_50330"
     CDS             3117..3350
                     /locus_tag="TUM9754_50330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87554.1"
                     /translation="MTEMEQQLLSALESLQRHYEQQQQAWQDSYANLQRMFEVTSQELA
                     KNDRVCQALSMQVTGLAQQVESLNRTVRRLSN"
                     /name="hypothetical protein"
     gene            complement(3395..3679)
                     /gene="relE_2"
                     /locus_tag="TUM9754_50340"
     CDS             complement(3395..3679)
                     /gene="relE_2"
                     /locus_tag="TUM9754_50340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000220578.1"
                     /codon_start=1
                     /transl_table=11
                     /product="RelE toxin"
                     /protein_id="BDO87555.1"
                     /translation="MSYNLEFDPRALKEWRKLGDDVRLQFKKKLEQVLQHPRIDKNRLR
                     ELHDCYKIKLRASGYRLVYQVRDQTITVFVVAVGKRERSAAYDAADKRL"
                     /name="relE_2"
     gene            complement(3669..3914)
                     /locus_tag="TUM9754_50350"
     CDS             complement(3669..3914)
                     /locus_tag="TUM9754_50350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002551444.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="BDO87556.1"
                     /translation="MMATINIRIDDELKSRSYAALEKLGVTPSEVLRQTLEYVAQSGRL
                     PFQQVLLTEDDADLMAIVRDRLENPQAGVKVSLDEL"
                     /name="transcriptional regulator"
     Typing          4039..4867
                     /name="rep_cluster_2486"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.278"
                     /coverage="80.0"
                     /evalue="0.0"
     Typing          1884..3397
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.604"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagcgtagca aaatggacaa ataagaatct tactgagagc ggttttagtg agtatgtaaa
       61 aaaaacacat actcctgata ttcaagcaaa aagagggcgt ttaggaggag aaaaaagtaa
      121 aggtggtgga aggccgtcca caacgggaaa gaaatggatc gaagaaggta ttagccgaag
      181 ctcatggtat agaaaaaaag ccactcagtt atgaatgagc ggctttatat gtaattgatt
      241 ttaaacgaag ttattgatta tgtaatcggc tagggcatca tgttcatcag acattgcttg
      301 cattaaatgt ttctgaggtc ctgaccaagc gccccttact aatacatctc ttgttgggcg
      361 aactgaagac aaatatcgaa tgggaacgcc ctgcaaaata ctatctggag ccattacgtt
      421 gagatcctct acttttgcta caggggtatc aggagtaaag gcggctactg atggatatgt
      481 ttttattgat gacagtttag gtcgaaggtc atttttgact gattcaataa ttttatctct
      541 gtgcgctgca tcggctgcaa tttctgtcgc gattgaatga cctggctgtt ttctcttgtc
      601 aaagccgtta aagatccaac cagcaaattt tggttgtcct ttttggctaa taagaggtgt
      661 gctttgagct gaacttcttc ttattccctg ctcccaatcg tctctaaatt tgggcaaggt
      721 ttcaccaatt agacctatgc aataagcgga gaacacatca gcagtacatg gaactaaata
      781 ataatcagag caatacagtg ctgatctaac aatagtatta aatgatggag gtacatcaat
      841 gagtacataa tcatactcac agtcgtttaa ttcttgagct ttatttgcga ttaagcttgg
      901 gattaaaaac cttgttactg cgttgcctgc gattagatca gtacccacgc taattgcgtc
      961 tgaaaggttg ttcaaccaaa aatcgccagg gactatgtct aaccgtcccg acccctcttc
     1021 cataggataa ttcggagtga aagtatgaac tgtcggggta atgtcttgtt gaataaaagg
     1081 ctggcaaaaa gctcttatgg tcttgccata cggattcgcc gcatcttttt ccaatagctc
     1141 ggagaatttt gtttctccga gtaatgcgat agacaaatta cactgcgggt cgaagtctat
     1201 cagtaaaacg gatttaccct tagccgatag ggacacactt agattccaag ttagagtagt
     1261 tttacccact ccccccttgt tattgaacag cgatattgtt ttcatttttc cctttattat
     1321 tcgtcgttag ttcgccacgt gagacaaaac aagcctatat cagataacag ctcctttcta
     1381 ggagttctct tctttttcgc gggatagtag actatacatg ctatagctta ttaagccagt
     1441 acacacagcg tcccctctgc gaggtgccgt ctgtgactgg ttcagggggc tcgccgcccc
     1501 ccgaaacccc ttgcatccac tgcgaaaatt cgcactttgg gtgcgaaact ttctcagcgg
     1561 attctcatgg aaaagcgcac caaagagatc aaaatcagac tcaccgaagc ggagcatcag
     1621 cggctacttg aacgctgtga ccgtaagcat ttggccgagt ggttacgagc cgttggctta
     1681 ggcgaatcgc gtacagctcg gcgtcgtccg ctacctaccg tagacccgac cttgttacgt
     1741 caggtcagcg ggatcggtaa taacctcaat caaatagccc gttacttgaa tcagcatggc
     1801 ttaccgccgc aagaacgggt gtcgttgtta gcggtgctca atagcattga ccaacatctt
     1861 gccgaactgc tggagcaaca tcgtgatcgt taagattcat ggtcgtggtg ccggtggcgg
     1921 gagtggtcct gtcgattacc ttctgggccc tgatcgtcag cgcgaacaag cgacggtgtt
     1981 acggggtaac cctgagcacg tcaaagagct gattgatggc tgcgaatttg cccgaactta
     2041 tacctctggc gtgctctctt ttcaggagag tgatttaccc gaaggcgaaa agcagcgatt
     2101 gatggatgaa tgggagcaga cattgatgac cggtctagat aaagaccagt atgcctgcct
     2161 ctgggttcaa catcaggaca aagggcgtct tgaattgaat tttgttatcc cgaacatcga
     2221 attgcagagc ggaaaacggc tacaacctta ctttgatcgg gctgaccggc ctcgtgttaa
     2281 cgcatggcaa accctcacca atgaccggct tgggttacgc gacccgaatg accctgctaa
     2341 tcgccgagca ttaaccccct ctaatgacct tcctcgcaac aaacagcagg cagcggaagc
     2401 cattaccaaa gggctaatca gcttgattga gcagggagaa attacggatc gtaaaggggt
     2461 gatttcccac cttaccgatg ccggattgtt ggtcgtacgg gaaaccaaat ccagtatcag
     2521 tattgctgat ccggcaggtg gcccgaatat tcgcttaaaa ggagtgctgt atgagcgaga
     2581 ttttaaattt agcgcgggag ttcgagagca aatcgaagca gcaagccaag actaccgcaa
     2641 cgagcgtcgc gaacgcattc gagaagcacg agaaacgtat caccgaggcc ttgaaattaa
     2701 gctcagggaa catacagacc gctatccaag aagagaacga cagccagcta aaacagatac
     2761 accgcttagt cggaatgaca tggctgtaca gcctggcatt aagtgggatc ctgtttgcga
     2821 tattgattgg agtagcttgg tatctcggga ctatcgtggt cgaacgccag aacgaaatca
     2881 gcgagcagag ccagatcctg caggacttaa agagccagac cggagccggc gtatcgataa
     2941 ttcacgattc caagaacaag agcgtgtatt acctgatcct tccgcagggg gcgaagaaga
     3001 tcgaagagta caagaacgct caacatcgtc aggtcatcaa gtacagcgcc aaataacctc
     3061 atcagacgcc acagaatcga ttctgggcgg ttttatctat cagggtgaag agattcatga
     3121 ccgaaatgga gcagcagctt ctgagcgcat tagagagctt acagaggcac tacgaacaac
     3181 agcagcaagc gtggcaggac agctacgcca acttacagcg catgttcgag gttacctcgc
     3241 aggagttggc gaaaaacgac agggtttgtc aggccttgag catgcaagtc accggcttgg
     3301 cgcagcaagt cgagagctta aacagaacag tgcgccgctt gagcaattag ccaagcggca
     3361 cgaacagcgg tattctcgcg gtcacggcat gagtttataa gcgtttatcg gccgcatcgt
     3421 aagcggcaga acgctcgcgc ttaccgaccg ccaccacgaa taccgtaatg gtttgatcgc
     3481 gaacctgata gaccaagcga taaccggatg cacggagctt gattttgtag cagtcatgca
     3541 gctctcgcag gcgattttta tcgatccgcg ggtgttgtag aacctgctcg agttttttct
     3601 tgaactgcag acggacatca tccccgagct tgcgccattc cttcagggct cggggatcaa
     3661 attcaaggtt atagctcatc cagtgacacc tttacgcccg cctgtgggtt ttccagacga
     3721 tcccgaacga tagccatcaa atcggcatca tcctcggtca gcaaaacctg ctggaacggc
     3781 aaacgtccgc tttgggccac atattccagt gtttggcgca gaacctcgga cggcgttacg
     3841 cccagctttt ccagtgcggc ataagagcgg cttttcagct cgtcatcgat ccgaatatta
     3901 atcgtggcca tcatctcacc tcttgatgta gtgacaagtg tatctacaag aagtagtatg
     3961 agcgtaaagc cgtgcgagaa caagcaggaa taacggattg tcggggatga caaaaaccgt
     4021 tgttgaggtg taacttagtg gcagaaaaaa caaagccccg aaatcatgct ctactttggc
     4081 gagcggacat gacattcagg gctaggtcga aacctagaaa ggatattagc acatgcagcg
     4141 tgcaaaacaa cagccccgcc ataaggctgg gagccttgga taatcaggct ttaacgcttt
     4201 ttaacgaccg gttaccccac aagccgtact tctccgatga tttacagttt ggtgttcgca
     4261 ttgccggtaa agagcgtgct ctcctcgcaa aatacatcca gtttaatcag cctcacgcca
     4321 tgtactggct ttgctttgac gtggacagag ccggagccgc gattgattgg gccgatctgg
     4381 gggcacctgc gcccacactc accatcaaaa accccgagaa cggacatgct cacctgttgt
     4441 atgccttgca tacggcggta cgtaccgcgc cggatggtcg agctgccccc cttaaatacg
     4501 ccgccgccat cgagaatgcg ctgcgtaaaa aattgggcgc agatgcgggg tattcagggc
     4561 taatttgcaa gaatccgaac cacctgcact ggcagatcac cgtctggcag ccagagctct
     4621 atacccttga ctggttagcc gattacctcg accttggcgc agccaatgac cgcgaaatcc
     4681 tgccggacta cggtttaggc cgtaactgca ccctcttcga taaaacccgc aagtgggctt
     4741 accgcgctat ccgccaaggc tggccgcagt atgaccaatg gctacaagcc tgcattgaac
     4801 gcgctaaagc ctacaatctg cagttctccg cacctttaga ggagaatgaa gtcatgggga
     4861 tagcaaa
//
