LOCUS       AP026493                5903 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM9754 plasmid pMTY9754_NA3 DNA, complete
            sequence.
ACCESSION   AP026493
VERSION     AP026493.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510791
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5903)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 230x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM9754"
                     /isolation_source="animal"
                     /db_xref="taxon:562"
                     /plasmid="pMTY9754_NA3"
                     /geo_loc_name="Japan"
                     /collection_date="2010"
     gene            complement(506..2014)
                     /locus_tag="TUM9754_50270"
     CDS             complement(506..2014)
                     /locus_tag="TUM9754_50270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87548.1"
                     /translation="MLRDFLKEKEIGCIQYVFVYPNIPKNKVDNFSKYFSNGFLSQEKV
                     CLFLDDTVFGSGKEGVLFTENYLCRKRIFEPSLSLLISDIIRMECKGRSLYVNGDCFYK
                     FSTVSGKDLQHIVNVMNEWIGEEKAQKLPGSINNVSDIEMNCAPRKEDSDERQPIVADM
                     LIKFLSSNNSFIVEWISKNFKERILDYIDSDDALNKLTFVIYGKMPSALMPFVKPEDVR
                     NFIAKNKVDFLNAIGLCGETENRANVIAGQTAAIDVCKCDDNYSEENVENKKTKEIKRG
                     EPENYELAFSLLRDDDSSVNNVLRFITKISLRHMWDVFNSSPFPPVYRVKYKLAYDFYN
                     AISQMLCSIDIDETDEAFELQMAASLAIFPVMVFASVVHIKDNYPITNDVLDLFIENVE
                     VTLNACCCFYNNDSNREFFRQMSVSFVENALSRGNVLINEFIDAGVKGISITREDLDLL
                     CSEAIYATDEWIDAISKIELSENKGYIYGWRELLCTSPLDAFIG"
                     /name="hypothetical protein"
     gene            complement(2254..3282)
                     /locus_tag="TUM9754_50280"
     CDS             complement(2254..3282)
                     /locus_tag="TUM9754_50280"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87549.1"
                     /translation="MFGSLLLGELSLRGVENPARKGFAKLGIVAQGGLLAVMSGVVGLA
                     VVSVFLGVKYSYPVFSQRLDAKVNIIADESLNVKPRRDDCFVSSGVTSPACVYGGNKVK
                     AILLGDSHADAVSSGLAASVLKNTDGILDLTYASCPTIFGLNVVSNDNSTKCKEFNSWA
                     KEKISAYSSDIPLVIVNRTSVYAFGQHNIKEQMNMPLSYFDIYNIKITDEFLNAYSDGL
                     IESACELAKQRPVYIMRPIPEMMVDVPKTMARALAFGKQSTEISISLEEYHARHKLVWD
                     AQDVAAARCGVKILDPLPYLCHDGRCWGSKNGRPIYYDDDHLSEYGNKLLVPMFKQVFV
                     VQ"
                     /name="hypothetical protein"
     gene            complement(4643..4966)
                     /gene="mobA_2"
                     /locus_tag="TUM9754_50290"
     CDS             complement(4643..4966)
                     /gene="mobA_2"
                     /locus_tag="TUM9754_50290"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000166966.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87550.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA_2"
     gene            5238..5432
                     /gene="rop"
                     /locus_tag="TUM9754_50300"
     CDS             5238..5432
                     /gene="rop"
                     /locus_tag="TUM9754_50300"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BDO87551.1"
                     /translation="MNKQQQTALNMAKFIKSQSLTLLEKLDSLDADAQAAMCEKLHELA
                     EELQNSIQTRFEAESGTGV"
                     /name="rop"
     Typing          complement(4976..5201)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="89.583"
                     /coverage="99.0"
                     /evalue="1.05e-76"
     Typing          6..352
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="81.843"
                     /coverage="83.0"
                     /evalue="4.12e-74"
     Typing          5476..5903
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.213"
                     /coverage="95.0"
                     /evalue="0.0"
     Typing          complement(4647..4966)
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.065"
                     /coverage="100.0"
                     /evalue="3.37e-62"
ORIGIN
        1 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
       61 cttatccggt aactatcgtc ttgagtccaa cccggaaaga cacgacttat cgccactggc
      121 agcagccatt ggtaactgga caaggtggat tgagatatta agagttcttg aagtggtggc
      181 ctaactgagg ctacactaga aggacagcat ttggtatctg tgctccacca agccagttac
      241 ccggttaggt aatctgccag gattagctaa ccttcgaaaa accacctgcc agggtggttt
      301 tttcgtttac aggcagcaga ttacgcttag aaaaaaagga tctcaagaga tcctttagtt
      361 ttttggctaa aagagcgctc acgattaagt cacacaacga ttgtcaacaa aaccagaaaa
      421 accgccgact ctagcggttt ttcttaaaaa gaagagataa cagcaaaaga aaagcttctc
      481 aagcttatta tttacttagt ataaattatc caataaatgc atctaatggc gatgtgcaca
      541 gcaattctct ccatccgtag atataacctt tattttctga taattctatt ttagatatag
      601 catcaatcca ctcatcagtt gcatatattg cctctgaaca taataaatcc agatcttctc
      661 tcgtaatgga tattcccttg accccagcat caataaactc gttaatcaaa acatttccac
      721 gcgataaagc attctcaaca aaagacactg acatttgtct aaaaaactct ctattgctat
      781 cattattata aaagcagcag catgcattaa gtgtaacctc tacgttctct ataaataagt
      841 ctaaaacatc attagttatt ggatagttat cttttatatg aacaacagaa gcgaacacca
      901 ttactgggaa aatagccaaa ctagcagcca tttgcaattc aaaggcttca tcagtttcat
      961 caatgtctat cgaacacaac atttgtgata ttgcgttata gaagtcatat gctaacttat
     1021 attttaccct atacacagga gggaatggag aggagttaaa aacatcccac atatgtctta
     1081 gagatatttt tgtaataaaa cgtaatacgt tattaacaga ggagtcatca tcacgcaaca
     1141 aagaaaatgc taactcataa ttttccggct cccctctttt tatttctttt gtttttttgt
     1201 tttccacatt ctcttctgag taattatcat cgcatttaca gacatcaatt gctgcagttt
     1261 gaccagctat aacatttgct cgattttcag tctccccgca taaaccaatg gcatttaaaa
     1321 aatcaacttt gttctttgca ataaagtttc taacatcttc tggcttaaca aaaggcatca
     1381 aagcagatgg catcttacca taaataacaa aagttaattt atttagcgca tcatcactat
     1441 caatataatc taaaattctt tctttaaaat tcttgcttat ccactcaaca ataaaactat
     1501 tatttgatga taagaatttt atcaacatat cagctacaat aggttggcgt tcatcgctat
     1561 cttcttttct gggtgcgcaa ttcatctcaa tgtcacttac attgttaata gaaccgggta
     1621 atttttgcgc tttttcttcg ccaatccatt cattcatcac attaacaatg tgctgcaagt
     1681 cttttccgct aacagtacta aacttataaa aacaatcccc attaacatat aaagaacgtc
     1741 ctttgcattc cattcttatt atatctgaaa ttaacagcga caagcttggt tcgaatattc
     1801 tttttcgaca taagtagttc tctgtaaata aaactccttc tttcccacta ccaaatacag
     1861 tgtcatcaag aaagagacag actttttctt gcgatagaaa accattagaa aaatattttg
     1921 aaaaattatc tactttattt tttggaatgt ttgggtatac aaacacatac tgaatacaac
     1981 caatctcttt ttcttttaaa aaatctctta acatcctatc accttcttaa tctactgtgg
     2041 ataaatgact attgcataaa gacataaagc atcacccaaa tcatcttcca aaagccagtt
     2101 ttagcaacga acaatattgt gtcattgcaa tagcatgcac ttccatgcga tacggcaaaa
     2161 cttggatatt taccataacg tcgcattaca cgcagttaaa tatagccttc ccatcaacat
     2221 tggcagccac catagcggct gccttggaaa ccactactgc acaacaaata cctgcttgaa
     2281 catcggaaca agcagtttat ttccatactc gctcaaatgg tcatcatcgt aatagattgg
     2341 tcgaccattt ttgcttcccc agcagcgacc gtcgtggcac aaatatggca gtggatcaag
     2401 aattttaacg ccacaccgag ctgcagccac atcttgtgca tcccacacca acttgtgacg
     2461 agcatgatac tcttcgagcg aaatagatat ttccgtggat tgttttccaa acgctagtgc
     2521 acgagccata gttttaggaa catcaaccat catttcagga ataggtcgca taatgtacac
     2581 tggtctttgc ttagctaatt cacaagcact ctcaatcaac ccatccgaat acgcattcaa
     2641 aaactcatct gttattttaa tattataaat atcaaaatat gatagtggca tgttcatctg
     2701 ttctttgatg ttgtgctgac caaaagcata cacagatgtt ctatttacaa tcactaatgg
     2761 aatgtcagag ctatatgctg atattttttc ttttgcccaa gagttgaact ctttgcactt
     2821 ggtgctgtta tcattactca caacattcaa cccaaaaata gttgggcaac tagcatatgt
     2881 taaatcaaga attccatctg tatttttcaa cacagatgct gccaaaccac ttgataccgc
     2941 atcagcatga ctatcaccaa gtaaaattgc tttaacttta tttccaccat aaacacaggc
     3001 tggagacgta acacccgagc taacaaaaca atcatctctt cttggtttta cattaagaga
     3061 ctcatcagca ataatattaa cttttgcatc cagtcgctga ctaaacaccg gatatgaata
     3121 tttaacaccc agaaaaaccg acactacagc cagcccaaca acaccggaca tcacagccaa
     3181 tagcccgcct tgcgccacaa tacctaattt ggcaaaacct ttacgagcag gattttctac
     3241 cccacgcagc gacaattcac ccaatagcag cgaaccaaac accccagcca caatccattg
     3301 agaattactt tgctcaccga tataagttag caataccact aacggccaat gccacagata
     3361 gatcgagtaa gagctagctc ctagacgact cgcaaaaaag ttagctgtta gccacgagcg
     3421 ctgtcgatta gcggacaaga ccaacatagc acccaccacc ggcactaacg caagatagcc
     3481 aggccatagc atcccccggt caaaggtggt aatagccaat ataatcagca caaaaccgcc
     3541 caattcagct aatgccgccc actgacgtgg catcggttga gttcgagtgc cccaccacac
     3601 caaaccaccg gctaacattt cccacgcacg agtcggtaat agataaaatg cagccccagg
     3661 ccaacgctca ctgacataga tagacaaagc cagtgacagc aatccagcaa caactagcgc
     3721 cagctttaca ccgcgtttac caaaccagtg ccacactaat aaacagcaca acggcaataa
     3781 aatataaaat tgccactcca ccgacagcga ccaagtgtgc aataaccatt tttcgtgact
     3841 tgcagcatca aaatagccag cttcacgcca gaacttgaca ttagaaataa acactaccgc
     3901 agttccaaca tgggatgcca acatcctgta gtcagcatta ggcaaccaga accaacccaa
     3961 tatcaataag ctgacgcaga gcaccaacag cgctggaata atccgcctcg cacgcgctaa
     4021 ataaaacccc cacagcgaaa agtttccacg ttcaagaccg gtgataataa tcccagtcat
     4081 taaaaagcca gagataacaa aaaatacatc aaccccaaca aaaccaccgg caaacccggg
     4141 cacgccaaag tgataaagta ccacggcaac aacagcccaa gcccgcaggc catttatgtc
     4201 gtagcgaaac gccttcggtg gattaaaagg ctgggaatca gctgattgag ttactttcat
     4261 aggatgaagt gacattccat aaccactatt tactacagag atttgtcaaa atggtcgaca
     4321 aacagcgcaa aaaaagggcc acattatgat tcaattgctt tctacgtgca ttaaagttga
     4381 tgttgctcgc cattttttat ccttagcagc taaacaagca aactatttac cataacgtca
     4441 cattacgcgt accgccaaaa cgcgctgaac gcgattctga cgcagacacc gcaaaaagag
     4501 atgttttctc cggggtaaac cggaaaaatg cgtcaggatc gctttcagct cactgcatag
     4561 ctatgcatga aagtgaatgg cgatcggttt gggggcttac ggcgttcata ccgtctgttt
     4621 tcgacagttt ctctccggga agctaatctg ccataagcct ggataacagg gcacggtgat
     4681 actccgtaat agcgatcagc acctccgcat actctctgtc cccgacacgc ttgccgtcga
     4741 taaagagttt tttctgcaac gcccccagtc gcataacttc tttcagcacc cggtcatcag
     4801 tcagtgagtt aactttctta ccgagagctg ccgcccgtaa aaaaccagaa acggtcttgc
     4861 cgcattcagc agcccttttt ctgatttcct ggtcttcttc tgccgtcaga cgaacagggc
     4921 gactgatagc ccgcctgcgc gtattacttc cacttcgttt tgtcataaaa catggctccg
     4981 tatctgacat ggggtgtcgg ggcgaagccc tgaccagggt aattgtaata gcgtgcatgt
     5041 atgcgcggta taacaattac acatcctgtc ctgtctgcaa gttcgaatta ccaacaaagc
     5101 actgctgttt tttggcagtg acagtgttgt ctgactgaaa tgtccgtcgg cagaataccg
     5161 gcggtaagac ggtcgaaaag tggtgcgcga tataatcaca ctacgcagat gacagacaga
     5221 acggagaaag cagaaagatg aacaagcagc agcaaaccgc actcaacatg gcaaaattca
     5281 taaaaagcca gagcctgacg ctgctcgaaa aactggactc actcgatgct gatgcacagg
     5341 ccgccatgtg tgagaaactg cacgaactcg cagaagaact ccagaacagc atacagacac
     5401 gctttgaagc ggaaagtggc accggggtat aacggtatat ccggcaccag acaggatgca
     5461 gaacggtata cctgtgcgta aggagaaaat gacacataag gcgctcttcc gcttcctcgc
     5521 tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggtgtcagct cactcaaagg
     5581 cggtaatacg gttatccaca gaatcagggg ataaagccgg aaagaacatg tgagcaaaaa
     5641 gcaaagcacc ggaagaagcc agcgacacag gcgtttttcc ataggctccg cccccctgac
     5701 aagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5761 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5821 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5881 tgttggtatc tcagttcggt gta
//
