LOCUS       AP026492                6222 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM9754 plasmid pMTY9754_NA2 DNA, complete
            sequence.
ACCESSION   AP026492
VERSION     AP026492.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510791
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6222)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 230x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM9754"
                     /isolation_source="animal"
                     /db_xref="taxon:562"
                     /plasmid="pMTY9754_NA2"
                     /geo_loc_name="Japan"
                     /collection_date="2010"
     gene            816..1559
                     /gene="aph(6)-Id"
                     /locus_tag="TUM9754_50220"
     ARG             724..1556
                     /name="aph(6)-Id"
                     /gene="aph(6)-Id"
                     /gene_name="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            complement(2114..2503)
                     /locus_tag="TUM9754_50230"
     CDS             complement(2114..2503)
                     /locus_tag="TUM9754_50230"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87544.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGHD
                     NSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANNVR
                     VGVTGTGWQCLATAGISAEGKRTFR"
                     /name="hypothetical protein"
     gene            2721..3161
                     /locus_tag="TUM9754_50240"
     CDS             2721..3161
                     /locus_tag="TUM9754_50240"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87545.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETSE
                     LIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMARE
                     AENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
                     /name="hypothetical protein"
     gene            complement(3928..4077)
                     /locus_tag="TUM9754_50250"
     CDS             complement(3928..4077)
                     /locus_tag="TUM9754_50250"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO87546.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPVG
                     REAL"
                     /name="hypothetical protein"
     gene            5266..6081
                     /gene="sul2"
                     /locus_tag="TUM9754_50260"
     ARG             5267..6078
                     /name="sul2"
                     /gene="sul2"
                     /gene_name="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /tool="amrfinderplus"
                     /drug_class="SULFONAMIDE"
                     /identity="100.0"
                     /coverage="100.0"
     ARG             2..723
                     /name="APH(3'')-Ib"
                     /gene="APH(3'')-Ib"
                     /gene_name="APH(3'')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="99.58"
                     /coverage="89.89"
     Typing          complement(4113..4286)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="99.429"
                     /coverage="100.0"
                     /evalue="1.19e-84"
     Typing          4210..4815
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.593"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac ctgcttcccg ccgcggtgag
       61 ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc gaggtgtggc ttgccccgag
      121 gtcatcaact ggcaggagga acaggagggt gcatgcttgg tgataacggc aattccggga
      181 gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag cgtggccgtc aatggggcag
      241 caacttggcg ctgttcacag cctatcggtt gatcaatgtc cgtttgagcg caggctgtcg
      301 cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg ccgtcaatcc cgacttctta
      361 ccggacgagg acaagagtac gccgcagctc gatcttttgg ctcgtgtcga acgagagcta
      421 ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt gccatggtga tccctgcatg
      481 ccgaacttca tggtggaccc taaaactctt caatgcacgg gtctgatcga ccttgggcgg
      541 ctcggaacag cagatcgcta tgccgatttg gcactcatga ttgctaacgc cgaagagaac
      601 tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc tattcaatgt attggggatc
      661 gaagcccccg accgcgaacg ccttgccttc tatctgcgat tggaccctct gacttggggt
      721 tgatgttcat gccgcctgtt tttcctgctc attggcacgt ttcgcaacct gttctcattg
      781 cggacacctt ttccagcctc gtttggaaag tttcattgcc agacgggact cctgcaatcg
      841 tcaagggatt gaaacctata gaagacattg ctgatgaact gcgcggggcc gactatctgg
      901 tatggcgcaa tgggagggga gcagtccggt tgctcggtcg tgagaacaat ctgatgttgc
      961 tcgaatatgc cggggagcga atgctctctc acatcgttgc cgagcacggc gactaccagg
     1021 cgaccgaaat tgcagcggaa ctaatggcga agctgtatgc cgcatctgag gaacccctgc
     1081 cttctgccct tctcccgatc cgggatcgct ttgcagcttt gtttcagcgg gcgcgcgatg
     1141 atcaaaacgc aggttgtcaa actgactacg tccacgcggc gattatagcc gatcaaatga
     1201 tgagcaatgc ctcggaactg cgtgggctac atggcgatct gcatcatgaa aacatcatgt
     1261 tctccagtcg cggctggctg gtgatagatc ccgtcggtct ggtcggtgaa gtgggctttg
     1321 gcgccgccaa tatgttctac gatccggctg acagagacga cctttgtctc gatcctagac
     1381 gcattgcaca gatggcggac gcattctctc gtgcgctgga cgtcgatccg cgtcgcctgc
     1441 tcgaccaggc gtacgcttat gggtgccttt ccgcagcttg gaacgcggat ggagaagagg
     1501 agcaacgcga tctagctatc gcggccgcga tcaagcaggt gcgacagacg tcatactaga
     1561 tatcaagcga cttctcctat cccctgggaa cacatcaatc tcaccggaga atatcgctgg
     1621 ccaaagcctt agcgtaggat tccgcccctt cccgcaaacg accccaaaca ggaaacgcag
     1681 ctgaaacggg aagctcaaca cccactgacg catgggttgt tcaggcagta cttcatcaac
     1741 cagcaaggcg gcactttcgg ccatccgccg cgccccacag ctcgggcaga aaccgcgacg
     1801 cttacagctg aaagcgacca ggtgctcggc gtggcaagac tcgcagcgaa cccgtagaaa
     1861 gccatgctcc agccgcccgc attggagaaa ttcttcaaat tcccgttgca catagcccgg
     1921 caattccttt ccctgctctg ccataagcgc agcgaatgcc gggtaatact cgtcaacgat
     1981 ctgatagaga agggtttgct cgggtcggtg gctctggtaa cgaccagtat cccgatcccg
     2041 gctggccgtc ctggccgcca catgaggcat gttccgcgtc cttgcaatac tgtgtttaca
     2101 tacagtctat cgcttagcgg aaagttcttt taccctcagc cgaaatgcct gccgttgcta
     2161 gacattgcca gccagtgccc gtcactccca ctcttacatt gttcgcagta tctaggtaca
     2221 tgttattgcg tgcaccttca tgatgtataa tgttattaat gacagcagaa agatcgacag
     2281 aattaatatt cttcgtacct gggacagtta tcgggtgtcg gccagtgatg agaccaaagg
     2341 atgcagtata agatgaacca ctactattat catggccacc attaccacca ccgttaaaac
     2401 tacctcccga acttccagag ccgctatgac catcattgta ggggtcattt ggtcccatgt
     2461 gaccaaaacc accgacagta ccgctattta actctgtact cataaaatta tcctctttga
     2521 cttttaaaaa cagcaaaaac tgtatttata acaatgtgtt tatatacagt attcgaaata
     2581 ctacgcccgt caaaaccaca ccgtcaacag gttttttgtg tatttttcac gcctcacggg
     2641 cagcctcaga ccttttgtgt taaaacagta atatgttctg ctgttacaat acggtacaca
     2701 caggagacac tgtcactttt atggcatggc acacagttca agagacactg aaattaacag
     2761 gaaagtcgcg ctcgcaatta tatcgtgaca tggctaaagg ccttgtcagc tatcgcacag
     2821 gaaaaaatga tcgccgggag ttcgagacca gtgaactgat tcgggcctat ggcgagctgc
     2881 gcacttctga gacaccaaaa aggcacaagg agggacactc caaatctgtc gttagccagg
     2941 cggaaaatgc tcagctagac gtcattcagc agaaactgga cgctctgcag cagactgtta
     3001 ccctaatgct tgaagacaag atggcgcggg aagcagaaaa ccgcctgcga aatgaggaga
     3061 gggaacaact acaggccgaa attagccgtc tggctgacgc gctggcacag gaaaaaaagc
     3121 ggggattctg gtcgcggctg tttcgctccg gttcagagta acagtgcagg tgactctgat
     3181 ttaccataac atctgttatc agcaccgcct gagaggccct cagggcgttt ctgacgggaa
     3241 accggtaaaa caggcaacaa accacccgaa aacccgccag aaacgcgcag gctctgtttt
     3301 tacggcatgc atgagtatgc gcacaagtgc atagcgcgga aattcacacc attcagccgg
     3361 ggtaagtcct gtttccggcg ttcttcccat ccgaaaacgg tgaacgggga cgcaatttat
     3421 tgcgtggccg tgaagcgttg ccggcgcagc cggaaaaccc gctgtcagca cgttcaggaa
     3481 gggggtgatg tacaaatggc acagaggatg aaagtgacgt atgagctgaa ggaatgatgt
     3541 aaaagtgatg tataagtgat gtaaaagtga tgtataagtg atgtaaaagt gatgtatgaa
     3601 ctgaaggagc gatgtaattc tgtagtcagg aagaataacc cgttcatgca ggcatgaacg
     3661 atacagtgag gaacgctttt tttgtcacga tctcccctta acctgcgtaa atcacccgcc
     3721 atggttgatt atttttcaac ctcaaaatgg tcatacacct aacgcagccg cgattttcat
     3781 cgcaactgca tgaattaaaa gaaaaaaaag gaatatttcg ttttcacgag taatacccgg
     3841 gtattaaaaa tccactgtaa ttacatgaaa ataaagaaat aaaaggtaat accggagtat
     3901 taccacgtac gcgggcaagc gaaaaagcta taacgcttcc cggccgacag gcgacccaaa
     3961 atccacaggg gcatgcaggt tctcttcagt aacctgcgcc attaaatcaa aaaggtcata
     4021 ttcgccggga agctgcgtat cagtcgaatc cagttgttca tcatattcag atggcatggg
     4081 gtgatatcca gtagctgaga ggttgttagc gggtttcggg gcgcagccct gaaccagtca
     4141 tgtagcgcta gcggagtgta tactggctta ctatgttgcc acgattgcga aaatcagtga
     4201 agtgcttcat gtggcaggag aaaaaaggca gcaccggcgc gtcagcagaa tatgcgatac
     4261 aggagatatt ccgcttcctc gctcactgac tcgctacgct cggtcgttcg gctgcggcga
     4321 gcggtaccgg cttacggacg gggacggaaa tttcctggaa gataccagga agacacttag
     4381 caaagaagag ataaggccac ggcgaagccg tttttccata ggctccgccc ccctgacaag
     4441 catcgcaaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatcc
     4501 caggcgtttc cccctggtag ctccctcgtg cgctctcctg ttcctgcctt tcggtttacc
     4561 ggtgtcattc cgctgttatg gccgcggttt ctcattccac gcctgacact cagttccgtg
     4621 aaggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     4681 gccttgtccg gtaactatca acttgagtcc aacccggaaa gacacgacaa aacgccactg
     4741 gcagcagcca ttagtaacag gagtgcacga gaagatagat taagaggtct tgaagtagag
     4801 gcctgactgc ggctacactg aaagaacagt tttggtaatg gcggtctcgt gaaccctgtt
     4861 accgggttaa gcagttcccc aactgactta accttcgaac aaaccgcctc cccaggcggt
     4921 tttttcgttt acagagcaag agattacgat tcattgaaaa ggatctcaag aagatccttt
     4981 gtagattact atcaattaat cttccgaaca tgacaccagt cactgttgat tacggttaaa
     5041 ccgctccgta acaggtttcc ctcgtattgg tgacttgcag caccaaagaa gcaaaaaaga
     5101 taaatatatc tacttattga gagatgatat atttatttcc aaaatgctac agatacatca
     5161 tcatgaaacg gatcaccgca aacaggttac tcgcctgggg attccctttc gacccgagca
     5221 tccgtatgag actcatgctc gattattatt attatagaag cccccatgaa taaatcgctc
     5281 atcattttcg gcatcgtcaa cataacctcg gacagtttct ccgatggagg ccggtatctg
     5341 gcgccagacg cagccattgc gcaggcgcgt aagctgatgg ccgagggggc agatgtgatc
     5401 gacctcggtc cggcatccag caatcccgac gccgcgcctg tttcgtccga cacagaaatc
     5461 gcgcgtatcg cgccggtgct ggacgcgctc aaggcagatg gcattcccgt ctcgctcgac
     5521 agttatcaac ccgcgacgca agcctatgcc ttgtcgcgtg gtgtggccta tctcaatgat
     5581 attcgcggtt ttccagacgc tgcgttctat ccgcaattgg cgaaatcatc tgccaaactc
     5641 gtcgttatgc attcggtgca agacgggcag gcagatcggc gcgaggcacc cgctggcgac
     5701 atcatggatc acattgcggc gttctttgac gcgcgcatcg cggcgctgac gggtgccggt
     5761 atcaaacgca accgccttgt ccttgatccc ggcatggggt tttttctggg ggctgctccc
     5821 gaaacctcgc tctcggtgct ggcgcggttc gatgaattgc ggctgcgctt cgatttgccg
     5881 gtgcttctgt ctgtttcgcg caaatccttt ctgcgcgcgc tcacaggccg tggtccgggg
     5941 gatgtcgggg ccgcgacact cgctgcagag cttgccgccg ccgcaggtgg agctgacttc
     6001 atccgcacac acgagccgcg ccccttgcgc gacgggctgg cggtattggc ggcgctgaaa
     6061 gaaaccgcaa gaattcgtta actgcacatt cgggatattt ctctatattc gcgcttcatc
     6121 agaaaactga aggaacctcc attgaatcga actaatattt tttttggtga atcgcattct
     6181 gactggttgc ctgtcagagg cggagaatct ggtgattttg tt
//
