LOCUS       AP026468                3372 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM1887 plasmid pMTY1887_NA4 DNA, complete
            sequence.
ACCESSION   AP026468
VERSION     AP026468.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510748
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 171x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1887"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1887_NA4"
                     /geo_loc_name="Japan"
                     /collection_date="2003"
     gene            complement(15..353)
                     /locus_tag="TUM1887_48970"
     CDS             complement(15..353)
                     /locus_tag="TUM1887_48970"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO63552.1"
                     /translation="MKREKMLTIRVTDEEHARLLERCEGKRLAEWMRRVCLGEPVARTG
                     KLPTLSPPLLRHLAAIGNNLNQTARKVNSGQWSSIDRVHVVAALMAIEGELRQLRQAVR
                     EQGVRDDS"
                     /name="hypothetical protein"
     gene            402..593
                     /gene="rop"
                     /locus_tag="TUM1887_48980"
     CDS             402..593
                     /gene="rop"
                     /locus_tag="TUM1887_48980"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BDO63553.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNDLDADEQADICESLHDHA
                     DELYRSCLARFGDDGESN"
                     /name="rop"
     gene            complement(1909..2142)
                     /locus_tag="TUM1887_48990"
     CDS             complement(1909..2142)
                     /locus_tag="TUM1887_48990"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO63554.1"
                     /translation="MTELEGHLLNALEHLQQDYMRRLNEWESAFAELQKMHEVTQRNNA
                     ILNERVVILSQQVQRLAGQVDRLSRLFIANNS"
                     /name="hypothetical protein"
     gene            complement(2149..2709)
                     /locus_tag="TUM1887_49000"
     CDS             complement(2149..2709)
                     /locus_tag="TUM1887_49000"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO63555.1"
                     /translation="MSNLLQMGTDFEKKLKERAASTENMLNSEFRKLEESVDKALSLNR
                     QKIRDAISEHTTSVKKQLDTLSTTVSMQFSTTEAELSRQQKKLLWRVIKGRILFPALTA
                     LSVTGGIFLGCWGLMEWQESKIAKNILTIREQENTLAKLEAKTWGVTFVNGENGKFLVL
                     PDGVKGENTWTVGDKNAVRLVRE"
                     /name="hypothetical protein"
     Typing          complement(675..755)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.805"
                     /coverage="93.0"
                     /evalue="8.68e-18"
     Typing          complement(19..371)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="84.746"
                     /coverage="100.0"
                     /evalue="9.42e-59"
     Typing          complement(1827..3370)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.692"
                     /coverage="98.0"
                     /evalue="0.0"
ORIGIN
        1 ccctggcatg aaatttaact atcatcccgt accccctgtt ccctgacagc ctgccgcaac
       61 tggcgcaatt caccctcgat ggccatcagt gcggccacca catgaacccg gtcaatcgaa
      121 gaccactggc cgctgttcac cttccgggct gtctggttca ggttgttccc gatggcagcc
      181 agatgccgca gcaatggcgg cgatagtgtc ggcagttttc ctgtgcgtgc aaccggctcc
      241 cccagacaga cccgccgcat ccattccgcc agtcgtttac cctcgcagcg ttccagtaac
      301 cgggcgtgct cctcatcggt aacccgtatc gtgagcatct tctcgcgttt catcggtatc
      361 attcccccta acaaatcccc catacaagga gccttaagac catgaccaaa caggaaaaaa
      421 cagcccttaa catggctcgc tttatcagaa gccagacgct aaccctgctg gaaaaactca
      481 acgatctgga cgcggacgaa caggcagaca tctgcgaatc acttcacgac cacgccgacg
      541 aactctaccg cagttgcctc gcacgcttcg gcgatgacgg tgaaagcaac tgaaacatgc
      601 agctcccgga gacgttcaca gcttgcctgt gagcggatgc cgggagcaga caagcccgcc
      661 agggcgcgtc agtgcgggtg tcggggcgca gccctgaccc agttcacaga gcgatagcga
      721 tgtgtatact ggcttatctc attttgcatc ggtgcagaat ggatgggaag tgcaccatgt
      781 caggagtaaa aagccgcaca gatgtaaaaa cagcgcaccc gataaacagc acacacgcgg
      841 acactggcac aggcgcgtaa gtgcacaatg cttgcatctg tgactcttcc gcttcctcgc
      901 ccactcgctc actacgttcg gtcgctgggg ctgcggcgag cggtgggatc actctcgttg
      961 tgatgctgat ccgttttttt tttttgcact ggagttatca ggagttgcgg tgcacatggg
     1021 atcgtgcgat atgccggatg gtgaaaggtt ggagggaacg gcaacaactc acccctccca
     1081 gtgaagggac gggtagtttg ccgctctcga tatgccggac ggagccaagg ccatcgatca
     1141 cggggcgcgt cagtggaaga acccgcgcct gcgtgacgta tgcgtcttcg ccgcaccatc
     1201 tcacccgtca cgccaccaca taccgggaac tgccgccaag gtctaactca accaatctag
     1261 ccgcgctaca tacggttttt gtggcaactc atcaggtgtc cgctgtcgat gaaactctct
     1321 gcgccggtag cggaactctc acgcgtgcgg tcgccgtcgt cacagctccc ccaaccgcaa
     1381 acgattctcg caaatacgca agtatctgca aaaacaggtt tttttcgggg ttcctgggag
     1441 actgtgctgt tgaaaaattc ttgaagaata cagaaggctc atgcaaactg aatgaactgg
     1501 tttctgttgt ggatctcaac agtttccaca ggaacattga ggaggtcaga gacctcaatg
     1561 aacccgaaac agctcgcctt cctggcgagc tgttttcgtt ctacgcccgc cgacgaaaga
     1621 tcgcaagtgc aataacgaga tttttcacgt ctttacgctc aaaaagtaaa aaaaccatgt
     1681 tttcccctgg ctgaatcccc aaaaacgcgt gaaatgcgtt ttcaggatgt tgcatagcta
     1741 tgcacaaata cggcacacga tttgtttcgc tcgccccagc cgtcgaactg gtcaaaatgg
     1801 caagatttcg tcacttcccc ggtcacatct ccattgatgg cccactccga acccgtgccg
     1861 cgcgttcctg tacctcatgt ctgtaccggg cacgctcctg ttcatccctt agctgttgtt
     1921 cgcgataaat aaccgactca agcgatccac ttgcccggct aatctctgca cctgctgact
     1981 caatatcacg acgcgctcgt tcagtatcgc gttgttccgt tgcgtaacct cgtgcatctt
     2041 ctgcaattcc gcgaaggcgc tctcccactc gttcagccgc cgcatatagt cctgttgcaa
     2101 atgctctaag gcgttcagca aatgcccttc cagttccgtc atactcgttt actccctgac
     2161 cagcctcacg gcgttcttgt cgcccaccgt ccaggtgttt tcccctttca ccccgtccgg
     2221 caacaccaga aatttcccgt tctcgccatt gacgaacgtc accccccagg tttttgcctc
     2281 gagtttcgcc agcgtgtttt cctgctctct gatagtcagg atatttttcg caatcttgct
     2341 ttcctgccac tccatcagcc cccagcaccc caggaaaatg ccgcccgtga cgctgagcgc
     2401 ggtcagcgcc ggaaacagta tccgcccttt gatcacccgc cagagcagtt ttttctgctg
     2461 ccgggacagt tcggcctctg tggtgctgaa ctgcatgctc acggtggtgc tcagcgtatc
     2521 cagttgcttt ttcacggatg ttgtatgttc gctgatagcg tctctgattt tctgcctgtt
     2581 taagctcaat gccttgtcta cagactcttc gagcttcctg aactcgctgt tcagcatgtt
     2641 ctcggtagaa gcggctcgct ctttcagttt cttctcgaag tctgttccca tttgtaaaag
     2701 attgctcata cagcgccccc tttagcctga gattccgccc cccctccggg tcggcaatac
     2761 tgatgctgct tttggttgtc ctcaccacct caaaacctgc cgctgtaagc gcctctgtga
     2821 catcctgacg cgtttttatt gctccggcat ggtaaagagc ctcaagaccc cgtgtaatcg
     2881 cttctgcggc ctcctgtttc gcttttggca gattatttgg cgtgacaagc gtccgtctgt
     2941 tctccggtgc gttcgggtca cgcagcccgt aatgatgatt aaccagcgtc tgccaggcat
     3001 ttatcctcgg cctgtccgcg cggtcgtagt acggctggag gcgcttaccg ctctgtagct
     3061 ccatgttcgg gatgacaaaa ttcagttcga gccgcccctt atcctggtgt tccacccaga
     3121 gaatgctgta ctggtctttt tcgagaccgg gcataagaac acgctcaaag ctcgtcatca
     3181 ctctttcgcg ttcacccggc ggtaactcct tctccgcgaa cgacagaacg cctgaagtgt
     3241 atttcttcga gaatggcgtg gaatcaatga gttcccgcac ttcttccggg ttaccccgga
     3301 gcaccgttgc gccttcgcgg ttacgctccc gccccagcag gtaatcgacc gggccacttc
     3361 caccgccttt cc
//
