LOCUS       AP026461                5589 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM1886 plasmid pMTY1886_NA6 DNA, complete
            sequence.
ACCESSION   AP026461
VERSION     AP026461.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510747
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 238x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1886"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1886_NA6"
                     /geo_loc_name="Japan"
                     /collection_date="2003"
     gene            complement(1036..1326)
                     /locus_tag="TUM1886_57000"
     CDS             complement(1036..1326)
                     /locus_tag="TUM1886_57000"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO58653.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVSRIQPLIDELNALPKPVPKEVSENDQQA"
                     /name="hypothetical protein"
     gene            1505..3664
                     /locus_tag="TUM1886_57010"
     CDS             1505..3664
                     /locus_tag="TUM1886_57010"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO58654.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPRELQQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAVGPAGFGGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNSGLTEPARRVADLLNIVLPDNATSYTLRDIIEALPKDSN
                     AAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKMEKQDIPASQTAFETQWQQWGASGLAALREQL
                     RKREEELRRKEQEETERRRAERRAEQLRKNDNAVMDSGVLSAVVTGYGEAPMPGNGEMT
                     CYLLLHNRNGEYTLWGNELEKYRTRIFECVDLMRDRSGYICDRSEIVQHPPLQRVYSSA
                     TFEQLLTQVCQTWPQYTRNLRQPKTWPESFCLGEDRQPAIPSPAARKVDFMQGRLPPTL
                     MPVMSSVDRETRQLQLLLVMGVDDSLGGVVRLNGTLYPAFAVPSADNRHLVISALTDKG
                     LRYAGYGVAVNYDADSQISPAPELMKFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRT
                     WDEWRRDEQHRKQERYPGNHRGMSM"
                     /name="hypothetical protein"
     Typing          complement(1328..1504)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.315"
                     /coverage="100.0"
                     /evalue="5.65e-83"
     Typing          1505..1863
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.778"
                     /coverage="100.0"
                     /evalue="1.83e-175"
     Typing          complement(2..504)
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.302"
                     /coverage="100.0"
                     /evalue="8.18e-162"
     Typing          1419..3658
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.181"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 atcatcgagg gcgataatac gttcccctgt gctgcggaat tgctggagaa gttcgtatat
       61 tcttatgctg taacttcttg ataaattgga gatattctta attacaactc tagtaaattg
      121 ccccttcaac tgtgtcagat aaggcatgac cgcatctgaa aaagtgattt gggctttgcc
      181 ctcaccttta gcgtactgtg cccgatactg gatccagcgg aactcctcgc gcttctcatc
      241 gtccttaagg atgatcgacc tgtcccacaa acgatcaatt gcttcctgta gctgaacctc
      301 ggcatttttc cgccccatat cagggaatga gtcgaaatac tcttcggcgg taacagtcat
      361 ggtcttttgc aattcagtgg attctgcatc agaacccgac ttcaatcgac caatgcaaag
      421 taacaagaat cgctgttcct gaagagtcaa tttatagctg gcctcaacca gtgcattgga
      481 tttgtaggcg atcagatccg acattgtaaa tccctcaacc taaggcaatg agtaaaatat
      541 aaacattcct ttaggtaaag gcaatatgat ttaattttaa tatccctttt cctgtggata
      601 ttaccttaaa tcatattgcc tttaccttaa atcatattgc ctatccacct taaatcatat
      661 tgcctttacc ttaaattata ttacctatag tcttctaaac tattgaattc cataagaaaa
      721 ttcgacccta aaacaagtat taaaaacaag atcttaaaaa caagatctaa aaacttaaca
      781 gcgagcctgt ggaaaacttg gggggcgtaa aaggaaaaaa aagcaaaaac aacatgttac
      841 aaaaaacagg ttcgcggact ttagtaaaac tcccgctcaa ccctgtggaa atccagtatt
      901 catgcgggtt aagcgcatat cacctgtcaa aaaaccgtgc gtatgtgctc aacctggcta
      961 cggttcactt ccgcttcctc gcccactcgc tcgctaacgc tcggtcgctg gggctgcggc
     1021 gagcggacag tgcattcacg cctgttggtc gttttctgac acttctttgg ggactggttt
     1081 tggcaacgcg ttcagttcgt caatcaacgg ctgtatcctg ctcacatcag cctgccgtgt
     1141 tacctctgcc ttcaggaact ggagtaacca ggctcgcaac tgcacttcct ccggctttct
     1201 gacctttgcc agcaatgctg cacctacaac aaccttctgc gctgtgtcca ggctccggct
     1261 cttcgccttc aggcgctgta atctcgcctc agcgtcagca atcttctgtt cgagcgttct
     1321 gctcatgatg taactccgtg cgcgatgaaa aatcgcattt tagcgcgtca ctggtagttt
     1381 aaaaactgag ctggcataat gcacggcaca tcacgaagtg cgcacttata taatcttcac
     1441 tccgtttcga ttgtgtgcgc cctgcggggc tgaaagaaaa ctacaaaaag gcattacggc
     1501 agaaatggcg atttatcatc tcagcatgaa aattatctcc agaagcagcg gctacagcgc
     1561 cgtagcttcc gctgcctatc gttccggttc actgatgctt gatgagcgta ccggactgac
     1621 tcacgactac acccgcaaaa gcggtgtggc tgaagctgtg attctgaccc ccgcgacagc
     1681 ccctgcctgg tgtacgaacc gtgccgaact ctggaatgcc gttgagaagg ccgaacgccg
     1741 gaagaactca caacttgcgc gggaaattga actcgctatc ccccgtgaac tgcagcagga
     1801 tgccgcacgg gaaaccgttc tcgcctttgt cagagaaaat tttgtcagtc agggcatgat
     1861 tgctgatgtg gcgttccatc acatggataa aacaaatccc catgcgcaca tcatgctgac
     1921 cacgagggct gtcggacctg ctgggttcgg cggaaaggtc agggactgga acgaccggac
     1981 acatgccgag acgtggcgtg catcatgggc tgaccatgca aacagagcac tggcgaacgc
     2041 tggttatcag gaagagatag accatcgttc atacgagcga caggggcttg agaaaacgcc
     2101 tggtattcac ctcggaaagt ctgcctgtgc gatggaaaca cgcggtatcg aaacagaacg
     2161 cggcgaacag aatcgtctga ttaacaggct taacctggaa atacagattt cccgcactga
     2221 actcaggaat agcgggctaa cagagcctgc ccgccgtgtg gctgaccttc tgaatatcgt
     2281 gcttcctgac aacgcaactt cgtacacact acgtgacatc atcgaagcgt tgccgaagga
     2341 cagtaacgcg gcatggaaac tcaccagtaa cgccatgtcg atgatggcag atatgcaggc
     2401 gacgcgcaga cgctgggaag agctgaacac cgaacgaaag gaagcgatga gccatgctgc
     2461 cagcctgaaa aaatcatcgc cgttttcgtc cggtttttcc cgtatacccc tgatgcaatg
     2521 ggctgcaccg gaataccgta gtgagcaggg aaaaatccag tctctgagcc agcaaatgga
     2581 aaaattgcga cagcattacc ggaagatgga gaaacaggac attcccgcga gtcagacggc
     2641 atttgaaaca cagtggcagc agtggggcgc atccggcctt gcagctctca gagagcagtt
     2701 gcgtaaacgt gaagaggaat tgcgccggaa ggagcaggag gaaacagaac gtcgtcgcgc
     2761 ggagcgacgg gcggaacagc tcaggaaaaa tgacaatgcc gttatggatt caggggtact
     2821 gagcgcagtc gttacggggt atggcgaggc ccccatgcct ggtaacgggg aaatgacctg
     2881 ttaccttctg cttcacaacc ggaacggaga atatacgctc tgggggaacg agctggagaa
     2941 ataccgcacc agaatctttg agtgtgttga tttaatgcgt gacaggagtg gttatatctg
     3001 tgaccgaagc gaaatcgtcc aacatccacc actgcaacgt gtttattcct ctgcgacctt
     3061 tgagcaactt ctgacgcagg tttgccagac atggccgcaa tatacccgca atctccgcca
     3121 gccgaagaca tggccggagt ctttctgtct tggagaagac cgccagccag ccataccatc
     3181 accagcggca aggaaggtgg attttatgca gggacgtctg ccaccgacgc tgatgccggt
     3241 catgagttct gtcgaccggg aaacccgcca gcttcagctt ttgctggtca tgggcgtgga
     3301 tgactctctg ggcggtgttg tccggcttaa tggtacgctg tatcccgcgt ttgccgttcc
     3361 ctcagccgat aacaggcatc tggtcatcag tgcgctgacc gacaaggggt tacggtatgc
     3421 agggtatggt gttgccgtca attacgatgc agacagccag atatcacctg caccggaact
     3481 tatgaagttt catctgaaaa cccgtgaggc accgcttttt gctgccgtca atacaccaga
     3541 aaaacagccg gatcatctgt tccgctctct gggttttaat cgtacctggg acgaatggcg
     3601 gcgcgatgaa cagcacagaa aacaggaaag atatcctgga aaccacaggg gaatgagcat
     3661 gtaggtatct ttggtgttat ccggatcgcc gtaaaccctg ccatgaagga cagattttga
     3721 cggcgatcag gataaaaaat gccataatga ttgtggcctt aaaattatcg aaaacacgaa
     3781 tttagtttta catttgtgcg cgtatgtacg cctcaaaaaa acaaccaacg aaaaacaact
     3841 catcaacaaa ataataacaa aaccttcttg ttttgtgatt tatgttgcac agtttgattg
     3901 taagtcacat ctgtgaaacg aaattgtttt gcgctatggc atctgatgag ataaaagaaa
     3961 gaggggtgtt tctgtatcgt aaagtctcta gccggatagc ttttcattgg tagtacagaa
     4021 acatccccac tcatcaggat tcaggatcga tagccattcg atataagact gctgattgtg
     4081 tcttcgttaa aatctatctt ttcacgtgag aattaagaac tgttaattca tcgtttgtat
     4141 tccctatcat taggataggg gatattttta tcgcaaaata cacaccaaat gtagtgttat
     4201 ataagttttc aatgaccgtt tcttactgaa aagaagatag gggattcgtg ttgacatcct
     4261 ccatgccctg aaggacgggg ttttacggcg caccgagtaa atcttccccc cgaaagccca
     4321 gtatcggggg gaagatgcat taacggttcg tcttcggata ctaccgtgat tgcggtgatg
     4381 agcatatcag ctcaacggga gaaattgaac gcgtttctga cagtgtggct aacacggcga
     4441 aatagacctc agatatctgc cagaaacgcc tcacgtattt ttaacgtgta tcaatatcca
     4501 tgtcgagata gttatccggt gtgctgtaaa accccgtcct tcagggcatg gaggatgtca
     4561 acttagatat ggataaaaaa aaccctcaat gaatgagggt aagtgtaagg aagtacctga
     4621 tttattttac atattgacca tcaataactg agcaaaaaaa aaataacaaa accatgacat
     4681 agatacaacc tatttttcct gtgcaagatt acctcgtttg ccataaaact ccgttcttca
     4741 gctcaaggaa aattttgaaa accatagctt atatcactat atgaatgaat atattgttgc
     4801 catattgcag tgtaatattt attatttttt gattgttttt agtttattat tttatttttt
     4861 ttctatattt ccaaagataa tctttttata ttctgtttct cgatactaga cataaaatca
     4921 aattcacgat aaatattaat tgcgcgtttc tgcccccccc acacctacta attactacct
     4981 gtgtttagtt acacaatcag aaacgcgctt tttaagcgtt aaaatagcgt gatgaactcc
     5041 tacagatatt caaatacctc tacgtcatcg tgaaaggacg cgctttgcca gaggcgaggt
     5101 tttgttttca agtgtgccct ggtgcttgat atccatcccg tcctgaaggt tggagattcc
     5161 tacgttgctc aggcttgctc tttagtcacg catgagtcac ttcgatgggg gactgctgct
     5221 ggcggcatta acgcatctct cacgtcaagt aaaacaagta atgttcgaca tggtttgcca
     5281 taccgctctc taaaatcgat tttgacgcaa tttatttgct tggtagtacc atcgcatacc
     5341 tgacaccaaa aaaacgaacc agatggcgtt ttagatgcct taaaatcgat tttagatcgt
     5401 catcttgatc tgtttgtctt ccttgaagcg aaaatgcagg gctacaaccg tgcgcccctt
     5461 cttgatcgtc tcaagcgtta cagccaagtc gcttttttga ttcagttcgt caacgcaagg
     5521 tcttaataat tgctggttca gagacttata tgtttgatat ttatgctcta tatcaagcat
     5581 tgagcgaaa
//
