LOCUS       AP026453                3751 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM1881 plasmid pMTY1881_NA4 DNA, complete
            sequence.
ACCESSION   AP026453
VERSION     AP026453.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510745
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3751)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 270x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..3751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1881"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1881_NA4"
                     /geo_loc_name="Japan"
                     /collection_date="2003"
     gene            213..1139
                     /locus_tag="TUM1881_55250"
     CDS             213..1139
                     /locus_tag="TUM1881_55250"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52951.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMPE
                     WARDNPSHFWQAADQFERANGSTYRELEIALPRELTAEQRLELVQDFVRQEAGERHAWS
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERTPEQYFRRYNARYPERGGAKKDSGSLT
                     LTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEAAGRL
                     DDAQRHDIMQKRSEPDYSRHLEQARTVGYDIREWLDTPVKQHELVRRLQHDVAREHGFD
                     MRDRVSRQNRTEPERVKSPSRGFSMGF"
                     /name="hypothetical protein"
     gene            1142..1675
                     /locus_tag="TUM1881_55260"
     CDS             1142..1675
                     /locus_tag="TUM1881_55260"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52952.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAMEQQKAVADSLAVARETLRGYRD
                     ISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIATIH
                     SNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTMLDRNYNATAAVYQKVVTLED
                     KLAEKPVTGKSRKN"
                     /name="hypothetical protein"
     gene            2109..3128
                     /locus_tag="TUM1881_55270"
     CDS             2109..3128
                     /locus_tag="TUM1881_55270"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52953.1"
                     /translation="MSAALQYFEENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQP
                     HAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASVKA
                     LKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREDAYTLDELADYLDLSASE
                     RRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPVPLSL
                     AECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSDKGKKS
                     AAVRWTANDDKRRRALDMYILGASTEDIAVAVGVSSRTIRRWMDNSGEWLTKKQIIKC"
                     /name="hypothetical protein"
     Typing          2110..2884
                     /name="Col156"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.907"
                     /coverage="83.0"
                     /evalue="0.0"
     Typing          214..1136
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.727"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 ccgtttcttc agccattcga tgttgctgat gcgcatgttc gtggtgctcc ctggtgtttt
       61 tttgcggtgt gttgtggtcg tgcagtatat cgcttgcggt cggcgggtgc agggggtatc
      121 ttccgtgcta attcgtaaaa ttagaaggaa gcgcacttat atgtttctct ccttcgtcga
      181 gaaacagtgc gctcctgcgg agggctgaaa ccgtggcaac ctatcacctc agcgttaaat
      241 ttggtggcaa ggggcaggct gcaaaccacg ccgattacat cgagcgcaaa gagaaatacc
      301 gcgaccgtca ggatttggaa tattccgcac acggaaacat gccggaatgg gcgcgagata
      361 atccctcaca tttctggcag gctgctgacc agtttgaacg tgcgaacggt tcgacctatc
      421 gggaacttga aattgcatta ccccgtgaac tgacagcgga acagcgcctt gaactggtgc
      481 aggattttgt acgtcaggaa gctggcgaac ggcacgcctg gagttttgcc attcacaatc
      541 cgaaagcgag cattgacggc ggcgaacagc ctcatgcgca tatcatgatg agccagcgtg
      601 tgaatgatgg cattgaacgt acaccggaac agtatttccg gcgctataac gccaggtatc
      661 ccgagcgggg aggtgcgaaa aaggattccg gaagcctgac tctcactcag caaaaagagc
      721 agttgcggga attgcgaaaa cgctgggagg tgaagcacaa cgaacatatg agaaagcatg
      781 gctttgaaag gggcttcatt gactgccgga cgctgaaaga acagggcatt gaacgacgtc
      841 ccgaagtgca tctgggtttt gaagctgccg gacggcttga tgatgcccag cgtcatgaca
      901 tcatgcaaaa acggtctgaa ccggactatt cccgccatct tgaacaggcg cgaacggtcg
      961 ggtatgacat cagggaatgg ctggacactc cggttaagca acatgaactg gtcagaaggc
     1021 tgcaacatga tgttgcgcgg gagcatggct ttgacatgag agacagagtg agcagacaga
     1081 accgaactga acctgaacgg gttaaatcac cgtcacgtgg attttctatg ggattctgaa
     1141 catggccagc acagaagaaa aattactcac gctcctgtta tcggtggagc atctgcaaca
     1201 taccgcaatg gagcaacaga aagctgttgc tgattctctg gctgtggcaa gggaaacact
     1261 tcgcggatat cgggatattt ctgacggtgt ggcaaaacag gtcagggatg aagtcagaaa
     1321 cgaggtgcag aagctggatg ttgcagggct gataggtgag cgcatcagcc aggattttca
     1381 gcggctggaa gagtcagtcg caaacatgaa ggcaaacgcg gatgccctga acagggatat
     1441 tgcgacaatt cacagcaata tcgtgcagga atacaacacc cttaaagggt atagctggaa
     1501 atggctgggt ggggtgtcag tggcttttct ggttatgttc tgtgtgcttg gatggggaat
     1561 gaaaacaatg ctcgacagga actacaacgc cacagcggcg gtgtatcaga aagttgtgac
     1621 actggaagat aaactggcgg agaaacctgt cacgggcaag agccggaaaa actgacgcgc
     1681 ttcgcttgtc caacgcctca cgacccgcga aaaaattccg cgtcccgttc accgttttct
     1741 gatgagaaag tttgctgttt tgacccgttc gcccttctga acgctcgaaa cagatcgcgc
     1801 atcgctttcg tgcatagtta tgcaacgcgc taaaaacgat tctgacgcat ttttctggtt
     1861 tagcctggtg tttccttgtc tttttgcgtt ttttgcgcca gagagcgact gagggcgttt
     1921 taaggggggc gtacaacggg agttatggta aatggatcgg ttttgcggga aggctcgaca
     1981 ggttttgttg ttgggggtag tgtaagcgac tgaaaaacaa acgcccctga aattcgtgtc
     2041 tctccgccaa gattgtcacg agaatacagg ggcgaggtcg gtcgataacc tggaaggaat
     2101 cgtaacacat gagcgccgcg cttcaatact tcgaagaaaa tttaccccac cgcccctatc
     2161 acacggatga tctcgctttc ggccttcgca tctccggcaa agggcgtgcg cttcttgcgc
     2221 ggtacatcca gcagaaccag cctcatgcgc agttctggct ggtttttgac gttgaccgcg
     2281 agggggctgc gattgactgg agcgaccgga acgcccccgc gccgaacatc accgttaaaa
     2341 atcctgtgaa cggacacgct cacctgctct acgcactcaa catcgccgtg agaaccgcgc
     2401 ctgatgcctc ggttaaggcg ctgaaatatg ccgcagcggt tgaacgttcg ctgtgtgaga
     2461 aactttgtgc tgatgtgaac tacagcggcc tgatttgcaa aaatccgttc cacctggaat
     2521 ggcaggtgat ggagtggcgc gaggacgcct ataccctcga tgaactggca gattatctcg
     2581 atttgagcgc ctcagagcgt cgtagcatcg ataaacatta cgggatgggg cgaaactgcc
     2641 acctgttcga aatgacgcgc aaatgggctt acagggcgat tcgtcagggc tggccagcgt
     2701 tctcacagtg gcttgaagcg gtgatccagc gtgtcgaaat gtacaatgca tcgcttcccg
     2761 ttccgctttc actggctgaa tgtcgggcta ttggcaagag cattgcgaaa tacacgcaca
     2821 ggaacttcac gccggaaact ttcgcacagt atgtggctga tacgcacacg ccagaaattc
     2881 aggcagcacg cggtcgcaag ggcgggaagg ccaattcgtc agaaaatcag tcggacaaag
     2941 ggaaaaaaag cgccgctgtt cggtggactg ctaacgacga caaacgtcgt agggcgcttg
     3001 atatgtatat cttgggagcg tcaacggaag atattgcagt ggcggttggt gttagcagcc
     3061 ggactattcg acgctggatg gataattctg gagaatggct aaccaagaaa caaataatta
     3121 aatgttagtt aacaaagaag taacactacc ccccgttttt tgttaatggg gggttaaatt
     3181 cgcggacatg aacaagccta tatcagataa cagcgccttt cggcgtcttt ttgagcagcg
     3241 ggctttccgg cggttttctc ctctcagccc agcaatggtg cggcttaccg catgattgag
     3301 gtggtcgtgc tcgccgcagt ctcatgaccg agcgtagcga gcgaatgagc gaggaagcac
     3361 cagggaaccg gaacacggat ggaggcactt acgcgtctgg gtttggggat gtctccagat
     3421 cgtacttaat ttccttaagt gcctgtaata tcctgtcttt tttatagcat agctcgcctg
     3481 tttttgtgtc taccagtcct gcttcgatca gtagcccagc cgcgagaaag agtcggtgat
     3541 tgcgttctcg tcgttgtttt tttccctcga ttcgtttttg gaccgcctgt tttcgttcgc
     3601 tctcctgtgc ctgtaagtcg tttttttccg ctgttgccag tacgtggagg agtttgcgtt
     3661 cctgttctgt gagtcctgct tcgttgtcga gcaggtcaat aatctgtctc tgtcgtgcgg
     3721 tctgttctcc gagcttcctg atgaatccga t
//
