LOCUS       AP026452                6062 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM1881 plasmid pMTY1881_NA3 DNA, complete
            sequence.
ACCESSION   AP026452
VERSION     AP026452.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510745
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 270x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1881"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1881_NA3"
                     /geo_loc_name="Japan"
                     /collection_date="2003"
     gene            complement(357..602)
                     /locus_tag="TUM1881_55160"
     CDS             complement(357..602)
                     /locus_tag="TUM1881_55160"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52942.1"
                     /translation="MAFYVDSTAKFLTKTTEPEGSVWRYYYKPGNTCPASGIYRCTNCG
                     DEVTCNKDDPLPPQNHRQHNVPGDIRWELVVMTKTK"
                     /name="hypothetical protein"
     gene            1301..2599
                     /locus_tag="TUM1881_55170"
     CDS             1301..2599
                     /locus_tag="TUM1881_55170"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002379013.1"
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="BDO52943.1"
                     /translation="MMVNFSLSNAFSFMEAQNMSMLAVSSSKDDINLANVVSVRDERIL
                     KSALIFGANASGKSNLMKTISLMKDIVLHSVQAIESSITKNVVPFLLDEAGPTKPSEME
                     MTFIADGIKYRYGLSLFQGKIKEEWLYYTPKTRETILFERDGQLIEINKAAFSESNLFI
                     KDGVIQKTREDVPFVSVVAGFNGEHSKRVVNWFSNVVFLSGSNEGMFTGITMKLINESE
                     DFKAWLKKILPAFQINDIYLDEVAPEFPFGNIKTDDDNLKNLFSSIQNFAKNQKVFSLR
                     VVKKTANGEISVPIDFESEGTKKIVYLLGPIYNAMINNNLLLVDEFDSKFHTLLSKFLF
                     RIFHAQSEGFSQVIAAVQDVNLMNTDCFRRDQIWFVDKDVKSGSSHLYSLVEYKEKQRS
                     LKPSYGNDYLSGAFDAIPLFDNLNEFEKLMPEG"
                     /name="abortive infection protein"
     gene            2604..3041
                     /locus_tag="TUM1881_55180"
     CDS             2604..3041
                     /locus_tag="TUM1881_55180"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52944.1"
                     /translation="MPKRGKSIRSSSRNEETKPSKSTAKTTDRFISISFKHFHNVDDVG
                     QSINTWKDDGSLPDLIEKLVYLTANGITKVISDGVFTNYTRFPESAVNDFRCPDEISSD
                     EDWGVIKNIGGQKRRVAGFLRDNVFYIVFFDRDHRFWKSQK"
                     /name="hypothetical protein"
     gene            3312..3473
                     /locus_tag="TUM1881_55190"
     CDS             3312..3473
                     /locus_tag="TUM1881_55190"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52945.1"
                     /translation="MSFRVGLKLIVTGKGAAVGLNGGFVHTVQLGANCLPGTECQAWNE
                     TNAAITAE"
                     /name="hypothetical protein"
     gene            3776..3955
                     /locus_tag="TUM1881_55200"
     CDS             3776..3955
                     /locus_tag="TUM1881_55200"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52946.1"
                     /translation="MSEEAEKRLDMHFLLTHLCGISHPAWRTFHTIRTDAAWLSQYTLR
                     YRYVTGSELRPDTR"
                     /name="hypothetical protein"
     gene            complement(4041..4232)
                     /gene="rop_2"
                     /locus_tag="TUM1881_55210"
     CDS             complement(4041..4232)
                     /gene="rop_2"
                     /locus_tag="TUM1881_55210"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BDO52947.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="rop_2"
     gene            4282..4620
                     /locus_tag="TUM1881_55220"
     CDS             4282..4620
                     /locus_tag="TUM1881_55220"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52948.1"
                     /translation="MKRERMLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSG
                     KLPTLAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVR
                     EQGVRDDS"
                     /name="hypothetical protein"
     gene            5298..5816
                     /locus_tag="TUM1881_55230"
     CDS             5298..5816
                     /locus_tag="TUM1881_55230"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52949.1"
                     /translation="MSNLLRTGAEFEKKLKERAESTEKMLNNEFRRLGESVSEAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDMLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQANLEDIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPCGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="hypothetical protein"
     gene            5823..6056
                     /locus_tag="TUM1881_55240"
     CDS             5823..6056
                     /locus_tag="TUM1881_55240"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52950.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="hypothetical protein"
     Typing          3869..3956
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.876"
                     /coverage="100.0"
                     /evalue="3.19e-37"
     Typing          complement(3318..3855)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.993"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          4611..6028
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.829"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 aacggttgat acaggagcgc gaacatgtgt tatccctgga acgggagcgt cagccggaaa
       61 tacaggaacg cacgctggat ggcccttcgc tgggatggtg aaaccatgaa aaatggcagc
      121 ttcagtggat taagtggggg gaatgtggcc tgtaccatct ggttgcatag gtattcatac
      181 ggttaaaatt tatcaggcgc gatcgcggca ttttttcggg tggtttgttg ccatttttac
      241 ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtacaatt cggatgttat
      301 ggtaaatcca gctgattggc aggtataaaa aagccgtccg tagacggcct agtaagttac
      361 ttcgtctttg tcatcactac caattcccat cggatgtcac ctgggacatt atgctgcctg
      421 tgattttgcg gtggcaaagg atcatcttta ttgcacgtca cttcatcacc acagttcgtg
      481 cagcggtaaa tgccggaagc cggacaagta tttcccggct tatagtaata ccgccataca
      541 ctcccttcag gctcggttgt ttttgttaaa aatttcgctg tgctatctac ataaaaggcc
      601 atgttgcttt ccttacataa actaagttag ggaccgaagc ttgcatgctt cggtggttat
      661 tgttttttct tttcccgatt aattgggatc ttaaacattc ccccgttagg acgggttatc
      721 cgtttgccct ttaccgttat gtaagggcga aaaatgtatg cctgaggctc gtccgtctgc
      781 atgttgcatt tcctattttc gccacatgaa tgttgatttt tgctatcaaa attgtactgt
      841 agcaaccaac agatcttgtg gctggcttta tcaaagcact tgaggtggta ctcaagcgcc
      901 gcatttgcgt atggtttgtt acgggttagt tctggtggta caggctaacc cacttacttc
      961 aacaattttt gcttctcctc attcatgttt cactccctac gtattttttt acagtgtcag
     1021 cgccactgag cataaacgcg acaaaaagga accatcccca cccagatacc gagtgaaaag
     1081 ccagcactgc tgagctaatc actgaggcaa atgggaccat ttggtataga aaaaacttca
     1141 tatacacctc tttttaagct ggtcgttaac ttatctactc ataatgtgct gtctattgga
     1201 cccttttgtt gttttagtac ggggttatgc tattcttaat ctagaatttt gtcaatattg
     1261 tgcaattttt gagtttaagg gtgttgttag gggtgttctt atgatggtta actttagtct
     1321 ttcaaacgca ttttccttca tggaagctca aaatatgagc atgttggcgg tgtcgtcatc
     1381 gaaagacgat ataaacttgg ctaatgtagt gtcagtaagg gatgagcgga tactgaagtc
     1441 tgctttgatt ttcggagcta acgctagcgg aaaatctaat ctcatgaaga caatatcttt
     1501 aatgaaagat attgtcctgc attctgtgca agctatcgaa agtagcatta cgaagaatgt
     1561 agtccctttt ttacttgatg aggcggggcc tactaaacca tctgaaatgg aaatgacttt
     1621 tattgcggat gggattaaat acagatacgg attatcttta tttcaaggga agataaaaga
     1681 agagtggttg tactacacgc caaaaacaag ggagactatt ctctttgagc gagatggtca
     1741 acttattgaa ataaataaag cagcatttag cgagtcaaat ctttttataa aggatggggt
     1801 cattcagaag acaagagaag atgttccctt tgtttctgtt gttgctggat ttaatgggga
     1861 gcactcaaag cgtgttgtga attggtttag taatgtggta tttctttctg gttctaacga
     1921 aggaatgttt actggaatta ccatgaagct tataaatgaa tctgaagatt ttaaagcttg
     1981 gctaaaaaaa atattaccag cctttcagat taatgatatt tatcttgatg aagttgcgcc
     2041 tgaatttccg tttggcaaca taaaaacaga tgatgataat ttaaaaaatt tattcagctc
     2101 gattcagaat tttgcaaaaa atcaaaaagt attttctctt agagtcgtga aaaaaactgc
     2161 taatggggag atttcggttc ccattgattt tgaatcagag gggaccaaaa agatagtcta
     2221 tctccttggg cctatttata acgccatgat taacaataac ttattattgg ttgatgagtt
     2281 cgattcaaag ttccataccc ttttatctaa gtttttattt aggatatttc atgcgcaaag
     2341 tgaagggttt agccaagtaa tcgctgcagt tcaggatgtt aatttaatga atactgattg
     2401 ctttagacga gaccaaattt ggtttgttga caaggatgta aagtcaggga gtagccatct
     2461 ttattctttg gttgagtaca aagagaaaca aagatcattg aagccatcat atgggaatga
     2521 ttatttatca ggtgcttttg atgcaatccc gttatttgat aatcttaatg aatttgaaaa
     2581 gctaatgcct gagggatgat tatatgccaa agagaggcaa aagcattcga agctcttcga
     2641 ggaatgagga aacaaaacct tcaaagagca cagcgaaaac gactgataga tttatatcta
     2701 ttagttttaa gcattttcat aacgtcgatg atgttgggca gtccataaat acatggaaag
     2761 atgatggtag cctacctgat ctcattgaaa aattggttta tctaacggcg aatggaatca
     2821 ctaaagtgat ttctgacggt gtgtttacta actacactcg atttcctgag tcagctgtaa
     2881 acgattttag atgcccagat gagatatcct ctgatgagga ttggggggtt ataaaaaaca
     2941 ttggcgggca gaaaagaaga gtcgccggat ttctgagaga taatgtgttc tatatcgtgt
     3001 tttttgatag agatcatcgc ttctggaaga gtcaaaaata gatgagatac agttgtattg
     3061 tatgttaaga aaagatcttc ttgagattgt gatgctctcg tcgtaatctc ttgctctgta
     3121 aacgaaaaaa ccgccttggc gggcggtttt tcgacggttc taagagttgg cgctctgtga
     3181 accaaggtaa ctggcttatg aggagcgcag taaccaaatc tgttctttca gtttagcctt
     3241 aacaggcgca taacttcaag actaactcct ctaaatcagt taccagtggc tgctgccatt
     3301 ggcgttttgt cgtgtctttc cgggttggac tcaagttgat agttaccgga aaaggcgcag
     3361 cggtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac tgcctacccg
     3421 gaactgagtg tcaggcgtgg aatgagacaa acgcggccat aacagcggag tgacaccggt
     3481 aaaccgaatg gcaggaacag gagagcgcac gagggagtca ccagggggaa acgcctggta
     3541 tctttaagtc ctgtcgggtt tcgccacctc tgacttgagc gtcggttttt gtgatgctcg
     3601 tcaggggggc ggagcctatg gaaaaacgcc agcggcgcgg ccttcctcct gttcctggtc
     3661 ttttgctcac atgttctttc cggccttatc ccctgattct gtggataact gtgttaccgt
     3721 ttttgtgtga gtcagtaccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag
     3781 cgaggaagcg gaaaagcgcc tggacatgca ttttctcctt acgcatctgt gcggcatttc
     3841 acacccggca tggcgtactt ttcatacaat ccgcactgat gccgcatggt taagccagta
     3901 tacactccgc tatcgctacg tgactgggtc agagctgcgc cccgacaccc gctaaaacct
     3961 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg ctcacagaca agctgtgacc
     4021 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat ccccgaaacg tgcgaggcag
     4081 ctgcggtaaa gctcatcggc gtggtcgtga agcgattcac agatatctgc ctgttcatct
     4141 gcgtccagtt cgttgagctt ctccagcagc gttaatgtct ggcttctgat aaagcgggcc
     4201 atgttaaggg cggttttttc ctgtttagtc actgatgcct ccgtgtaagg gggatttctg
     4261 ttcacgggag taatgatacc gatgaaacgc gagaggatgc ttacgatacg ggttactgat
     4321 gatgaacatg cccggttact ggaacgctgt gagggtaaac aactggcggt atggatgcgg
     4381 cgggtctgcc tgggtgagcc ggttgcccgt tccgggaagc tgccgacact ggcaccgccg
     4441 ttactgcgtc agctggccgc catcggaaat aacctgaatc agacagcccg taaggtgaac
     4501 agcgggcagt ggtcttccgg tgaccgggtt caggtggtgg ccgcactgat ggccatcggg
     4561 gatgagctgc gccggctgcg tctggccgtc agggaacagg gagtgcggga tgatagttaa
     4621 gtttcatgcc aggggaaaag gtggtggcag tggtccggtt gattacctgc tgggaaggga
     4681 gcgtaaccgg gaaggggcaa cggtgcttca gggtgatccg gaagaagtcc gggaactcat
     4741 cgatgccacg ccatttgcga agaaatacac gtcaggtgtt ctgtcgttcg cggagaagga
     4801 gctgccgccg ggagggcgtg aaaaagtgat ggcgagtttt gagcgtgtac tgatgcccgg
     4861 tctcgaaaag aatcagtaca gcatcctgtg ggtggagcac caggacaagg gacggcttga
     4921 gctgaatttt gtcattccga acatggagct acagaccgga aaacgcctcc agccgtacta
     4981 cgaccgtgca gacaggccga gaattgatgc ctggcagacg ctggtgaatc accattacgg
     5041 gctgcatgac cccaacgccc cggagaaccg ccggacgctg actctccctg ataacctgcc
     5101 ggaaaagaaa caggcgcttg ctgagagcgt tacacggggg cttgatgtgc tttaccgtgc
     5161 cggagagata aaaggccgtc aggatgtgat tcaggcgctc acagaggtgg gacttgaagt
     5221 ggtcagggtg acgcgaagca gtatcagcat tgcagacccg aacggcggga ggaatatcag
     5281 actgaaagga gcgttttatg agcaatcttt tacggacggg cgcggagttc gagaaaaagc
     5341 tgaaagagag agccgaatct acagagaaaa tgctgaacaa cgagttcagg aggctcggcg
     5401 aatctgtaag cgaggctgtg acatcaaacg agacgaaaat caaagacgct atagccctgt
     5461 tcaccgcctc gacagaggag tcgctgaaaa aacaccggga aggggtgaaa gaggcgatga
     5521 tgcagcacag gaaggacatg ttaaagctgg cagggaatac gggcatgatg ttactgggga
     5581 tagtctttct cctgtttacc gcgagtggcg ggacgctctg gtatcttggc gggaggatac
     5641 aggcgaacct ggaagatatc aggaagcagg aagagacatt gcagaaactg aacgcgaaga
     5701 catggggcgt ggagtttgtg caggacggga acaggaaatt ccttgtcctt ccgtgcggga
     5761 aatcagcgga ggtgattccc tttcagggga aagagtgggt acatctgaag gagtgacaca
     5821 gagtgacaga gctggaaata catttgctga acgccttaga gcagctgcaa caggactata
     5881 tgcagcggct gagcgaatgg gagagcgcct tcgtggaatt gcagaagatg ttttcgctta
     5941 cgcaacggga caacgcgatg ctgaacgagc gggtcatgca gttgagtcag caggtgcagc
     6001 acttgagcga gcagacagaa cgcttgagcc agttatacag cgagaactgg agataagaga
     6061 gg
//
