LOCUS       AP026451                6267 bp    DNA     circular BCT 23-JUL-2022
DEFINITION  Escherichia coli TUM1881 plasmid pMTY1881_NA2 DNA, complete
            sequence.
ACCESSION   AP026451
VERSION     AP026451.1
DBLINK      BioProject: PRJDB13833
            BioSample: SAMD00510745
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Ikegaya,K., Aoki,K., Komori,K., Ishii,Y. and Tateda,K.
  TITLE     Complete genome of blaCTX-M-2-producing Enterobacteriales
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Aoki,K. and Ishii,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JUL-2022) Contact:Kotaro Aoki Toho University,
            Department of Microbiology and Infectious Diseases Toho University
            School of Medicine; 5-21-16, Omori-nishi, Ota-ku, Tokyo 143-8540,
            Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.8-beta
            Genome Coverage       :: 270x
            Sequencing Technology :: Illumina MiSeq; ONT MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="TUM1881"
                     /db_xref="taxon:562"
                     /plasmid="pMTY1881_NA2"
                     /geo_loc_name="Japan"
                     /collection_date="2003"
     gene            477..920
                     /locus_tag="TUM1881_55080"
     CDS             477..920
                     /locus_tag="TUM1881_55080"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52934.1"
                     /translation="MTSGIFLSREDLMLLSDQAKEEVLALATGDSVSGVYEESEDGPTD
                     LSSLQAERLVRGLSDKSRTVLKAILESEDGERGFWCKSLAKELGFDVSELSGVWSGLTR
                     RTRTVSDDSEAYLISWAWDDDRDDYYGTLHPTTYRNCKKAMNF"
                     /name="hypothetical protein"
     gene            957..1139
                     /locus_tag="TUM1881_55090"
     CDS             957..1139
                     /locus_tag="TUM1881_55090"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52935.1"
                     /translation="MCGLFSLVRVVRRYGKSIWGGIGFWVALCPYDLVYESKVDMIFSA
                     IALITGTATEFPNCL"
                     /name="hypothetical protein"
     gene            complement(1058..2146)
                     /locus_tag="TUM1881_55100"
     CDS             complement(1058..2146)
                     /locus_tag="TUM1881_55100"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:A6H1I7"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine-specific methyltransferase"
                     /protein_id="BDO52936.1"
                     /translation="MTQKEFADALGLSKYGDRTIRRWERGETKPTGAELKAVMDFPDTP
                     PYPNNENGRYRMIDLFAGIGGTRLGFHQTNAVNVVFSSEWDKFAQKTYHANYGDFPDGD
                     ITKIDEKDIPDHEILVGGFPCVAFSQAGLKKGFNDTRGTLFFDIARIIKEKKPHAFLLE
                     NVKNLLGHDKGRTFSIIKNTLEELNYTVYYNIFAAKDFGVPQNRERIYIVGFNKEKVRN
                     HEHFTFPTPLKTKTRVGDILEKSVDNKYTLSDALWNGHQRRKLVNAAAGKGFGYGLFNE
                     NSPYTNTISARYYKDGSEILIEQKGSNPRKITPREASRLQGFPSDFIIPVSDTQAYKQF
                     GNSVAVPVINAIAEKIISTLDS"
                     /name="cytosine-specific methyltransferase"
     gene            2307..3224
                     /locus_tag="TUM1881_55110"
     CDS             2307..3224
                     /locus_tag="TUM1881_55110"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52937.1"
                     /translation="MQRLSPGEFKTLISKERKSHFITPFALVYKTFCDLGYDQKNSDYF
                     LNNPSEYIIAMRKNCWKEFEPFEKEFTTRMLSYLIDEERIKDMSPYDAIRDFTMEYPTH
                     IYDLALSNTQSRRSRAGKEFESILELLMMGAGIPVDVQGAIGKSFFQKNQIGKLVDLVM
                     PGVVQYTSNKRNTMLISAKTTLRERWQEVPEEVNRTGIREMYLATLDDSFSEETINILY
                     EANVVVVTTIENKNFKYKNNNRVLTFEDMLQSAMELSRKWNNVSYTDSEKEEIQQSILK
                     QIEKYSDFPYVVNYYRNRLSALVD"
                     /name="hypothetical protein"
     gene            complement(4247..4438)
                     /gene="rop_1"
                     /locus_tag="TUM1881_55120"
     CDS             complement(4247..4438)
                     /gene="rop_1"
                     /locus_tag="TUM1881_55120"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BDO52938.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="rop_1"
     gene            4580..4825
                     /locus_tag="TUM1881_55130"
     CDS             4580..4825
                     /locus_tag="TUM1881_55130"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52939.1"
                     /translation="MRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSGQ
                     WSSGDRVQVVAALMAIERELRSLRQVVREQGARDDC"
                     /name="hypothetical protein"
     gene            5503..6021
                     /locus_tag="TUM1881_55140"
     CDS             5503..6021
                     /locus_tag="TUM1881_55140"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52940.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLGESVSEAVTSNE
                     TKIRDAIALFTASTEKSLEKHREGVKEAMMQHRKDVLKLAGNTGMMLLGIVFLLFTASG
                     GTLWYLGGRIQTNLEEIRKQEETLQKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVIPFQ
                     GKEWVHLKE"
                     /name="hypothetical protein"
     gene            6028..6261
                     /locus_tag="TUM1881_55150"
     CDS             6028..6261
                     /locus_tag="TUM1881_55150"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BDO52941.1"
                     /translation="MTELEIHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDNA
                     MLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
                     /name="hypothetical protein"
     Typing          4077..4162
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.701"
                     /coverage="100.0"
                     /evalue="1.86e-34"
     Typing          complement(3506..4055)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.756"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          4473..4819
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.103"
                     /coverage="98.0"
                     /evalue="1.36e-63"
     Typing          4816..6233
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.252"
                     /coverage="90.0"
                     /evalue="0.0"
ORIGIN
        1 cacggctgat acaggagcgc gaacatgcgt tatccctgga acgggagcgt cagccggaaa
       61 tacaggaacg cacgctggat ggtccttcgc tgggatggtg aaatcatgaa aaatggcagc
      121 ttcagtggat taagaggggt taatgtggcc tgtaccatct ggttgcatag gtattcatgc
      181 ggttaaaatt tatcaggcgc gatcgcggca gtttttcggg tgatttgttg ccatttttac
      241 ctgtctgctg ccgtgatcgc gctgaacgcg ttttagcggt gcgtgtaatg cgacgttatg
      301 gtaaatccgg gctgttgagt gttgacctct cactatcgca acccagttaa tgcggtaaat
      361 caaacacaac tagctgttaa ttgatgttag ttgtattgac ttgtcacttg tcttgttgac
      421 aactatcttg tctatgttta ttctcctttt atcttaacaa gacaatggag tgtttcatga
      481 ctagtggtat tttcttatcc cgtgaagatt tgatgttgtt atcagaccaa gcaaaagaag
      541 aagtgctggc attggctact ggggattcgg tttctggtgt ttatgaggag tcggaagatg
      601 ggcctactga tctttcctcg cttcaggcag aaaggcttgt tcgtggttta agtgataagt
      661 cgcgaacagt gctgaaagct attcttgaaa gcgaggatgg tgagcgagga ttctggtgca
      721 agagcctcgc aaaggagctt ggttttgatg tctctgagct ttcaggtgtc tggtcagggc
      781 ttacacgcag aactcgcacg gtaagtgatg attctgaggc ttatttgatt agctgggctt
      841 gggatgatga ccgtgatgat tactatggaa cgctacatcc aacaacctat cgcaactgca
      901 agaaagctat gaacttttga ttgttctcgc tgtgatactt tccctgtgag cccgccatgt
      961 gcgggctttt ttcattggtt cgtgtagtga gacgttatgg taaatccata tgggggggaa
     1021 ttggattctg ggtcgcattg tgtccttatg acttagttta tgaatctaag gtcgatatga
     1081 ttttttctgc tattgcattg ataacaggga ctgcgactga attcccaaat tgcttataag
     1141 cctgtgtatc cgatacaggg ataataaaat cactaggaaa cccttgaagt cttgacgctt
     1201 cccgcggagt tatttttctt gggttacttc ctttttgctc tattaatatt tcgctaccat
     1261 ctttatagta cctcgcactt atggtattag tatatgggct attttcatta aataaaccat
     1321 acccaaagcc tttaccggct gctgcattta caagtttcct gcgttggtgt ccattccaga
     1381 gcgcatctga tagcgtatat ttattgtcta cgctcttttc aagaatatct ccaactcttg
     1441 tttttgtttt taatggggtt ggaaaagtaa aatgttcatg atttcttact ttttctttat
     1501 taaagcctac aatgtatatg cgttctctat tttgtggaac accaaagtct tttgcagcga
     1561 agatattgta atacacagtg taattcaatt cttcaagtgt gttttttatt attgaaaaag
     1621 ttctaccttt atcatgtcct agcaagtttt ttacattttc aagtaaaaaa gcatgaggtt
     1681 ttttttcctt tattatcctt gctatatcaa aaaacaaagt tcctcttgta tcattaaatc
     1741 cttttttgag ccctgcttga gagaatgcta cgcaaggaaa cccaccgaca agaatttcat
     1801 gatcaggaat atctttttca tcgatttttg ttatgtcccc atcagggaaa tcaccataat
     1861 ttgcatgata tgtcttttga gcaaacttat cccattcgct ggaaaaaaca acatttacag
     1921 cgttagtttg atgaaatcca agtctggttc caccgattcc agcaaataaa tcgatcatgc
     1981 gatatctgcc attctcgttg tttgggtaag gtggtgtgtc tggaaagtcc ataactgctt
     2041 ttagttccgc cccagtaggt tttgtttccc cgcgctccca tcttcttatg gttctgtctc
     2101 catattttga aagaccaagg gcgtctgcaa actctttttg agtcatatga agtctttctc
     2161 tttttccttt gattgttgct gcgatattat cagtcatgca tgtctaccag aattaagtgt
     2221 tggttttagg acaatttgtc ctgttttgat tccaattatg aacgtgcatg aaaaaacttt
     2281 caagtagata taaaaggtta ttttttatgc aaagattgag tcctggtgaa tttaaaactc
     2341 taatttctaa agagagaaag tcgcatttta ttacgccatt tgctttggtt tataaaacat
     2401 tttgtgattt agggtatgat caaaaaaata gtgattattt tttgaataat ccaagtgaat
     2461 atataattgc aatgagaaaa aactgttgga aagaatttga accatttgaa aaagaattca
     2521 caacaagaat gttatcatat cttattgatg aagaaagaat aaaagatatg agtccttatg
     2581 atgccattcg tgattttacg atggaatacc ctacacacat atatgatttg gcactgtcta
     2641 acactcaatc cagacgtagc agggcaggaa aagagtttga gtctattctt gagctattaa
     2701 tgatgggggc aggaattcct gttgatgtgc agggggctat tgggaagtca ttttttcaaa
     2761 aaaaccaaat aggtaaactt gtagatcttg ttatgccagg tgtagtccag tacacatcta
     2821 acaaaagaaa tacaatgttg atttccgcta aaacaacgct gagggagcga tggcaagaag
     2881 tacctgaaga ggttaataga acaggaatta gagaaatgta tctagctaca ctagatgatt
     2941 cttttagcga ggagacaata aatatattat acgaggctaa tgtggtcgtt gtaacgacga
     3001 tagaaaataa aaatttcaaa tataaaaata ataatagggt gctaaccttt gaagatatgc
     3061 tacagtcagc aatggaatta tcaagaaagt ggaataatgt aagttacaca gactctgaaa
     3121 aagaggaaat tcaacagtca atattaaaac aaatagaaaa atatagtgat ttcccttatg
     3181 ttgttaatta ttatagaaat aggcttagtg ctcttgttga ctaaaaaact aaaaggatct
     3241 cttgagatcc tttttttctg cgcgtaatct tggacctgta aacgaaaaaa ccaccctggc
     3301 aggtggtttt ttcgaaggtt agctaatcct ggcagattat ctaaccgagg taatctggct
     3361 tcagcagagc acagatacca aatactgtcc ttccagtgta gccgtagtta ggccatcact
     3421 tcaagaactc tgtaagcatc tggataaatc tcgctctgct aatccggtta ccagtggctg
     3481 ctgccagtgg cgttaaggcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     3541 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     3601 cctacaccgg gccgagatac caacagcgtg agctatgaga aagcgccacg cttcccgaag
     3661 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     3721 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     3781 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatgggaa aacgccagcg
     3841 gcgcggcctt ttgttgccct ttgccttttt actcacatgt tctttccggc cttatcccct
     3901 gattctgtgg ataaccgtat ttccgccttt gagtgagccg gcaccgcttg ccgcagccga
     3961 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt
     4021 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     4081 actgatgccg catggttaag ccagtataca ctccgctatc gctacgtgac tgggtcaggg
     4141 cttcgccccg acacccccta aaacctgctg acgcgccctg acgggcttgt ctgctcccgg
     4201 catccgctca cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     4261 cgtcatcccc gaaacgtgcg aggcagctgc ggtaaagctc atcggcgtgg tcgtgaagcg
     4321 attcacaaat atcggcctgt tcatccgcgt ccagttcgtt aagcttttcc agcagcgtta
     4381 atgtctggct tctgataaag cgcgccatgt taagggctgt tttttcctgt ttagtcactg
     4441 atgcctccgt gtaaggggga tttctgttca tggggtaatg ataccgatga aacgcgagag
     4501 gatgctcacg atacgggtta ctgatgatga acatgccagg ttactggaac gctgtgaggg
     4561 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     4621 gaagctgccg acactggcac cgccgttact gcgtcagctg gctgccatcg ggaataacct
     4681 gaatcagacg gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     4741 ggtggccgca ctgatggcca tcgagagaga gttgcgcagc ttacggcagg tcgtcaggga
     4801 acagggagcg cgggatgatt gttaaatttc atgccagggg aaaaggcggt ggcagtggtc
     4861 cggttgatta cctgctggga agggagcgaa accgggaagg cgcaacggtg cttcagggta
     4921 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtcgg
     4981 gtgttctgtc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaag gtgatggcga
     5041 gttttgagcg tgtactgatg cccggtctcg aaaagaatca gtacagcatc ctgtgggtgg
     5101 agcaccagga caagggacgg cttgagctga attttgtcat tccgaacatg gagctacaga
     5161 ccggaaaacg cctccagccg tactacgacc gggcagacag gccgagaatt gatgcctggc
     5221 agacgctggt gaatcaccat tacggactgc atgaccccaa cgccccggag aaccgccgga
     5281 cgctgaccct tcctgataac ctgccggaaa cgaaacaggc acttgctgag agcgttacac
     5341 gcggtataga cgcactttac catgccggag agataaaagg ccgtcaggat gtgattcagg
     5401 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     5461 acccgaacgg cgggaggaat atcagactga aaggagcgtt ttatgagcaa tcttttacag
     5521 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     5581 aacgacgagt tcaggaagct cggcgagtct gtcagcgagg ctgtgacctc aaacgagacg
     5641 aaaatcagag acgctatagc cctgttcaca gcctcgacag agaaatcgct ggaaaaacac
     5701 cgggaagggg tgaaagaggc gatgatgcag caccggaagg acgtattaaa gctggcaggg
     5761 aatacgggca tgatgttact ggggatagtc tttctcctgt ttaccgcgag tggcgggacg
     5821 ctctggtatc ttggcggacg gatacagacg aacctggaag aaatcaggaa gcaggaagag
     5881 acattgcaga aactgaacgc gaagacatgg ggcgtggagt ttgtgcagga cgggaacagg
     5941 aaattccttg tccttccgta cgggaaatca gcggaggtga ttccctttca ggggaaagag
     6001 tgggtacatc tgaaggagtg acacagagtg acagagctgg aaatacattt gctgaacgcc
     6061 ttagagcagc tgcaacagga ctatatgcag cggctgagcg aatgggagag cgccttcgtg
     6121 gaattgcaga agatgttttc gcttacgcaa cgggacaacg cgatgctgaa cgagcgggtc
     6181 atgcagttga gtcagcaggt gcagcacttg agcgagcaga cagaacgctt gagccagtta
     6241 tacagcgaga actggagata agagagg
//
