LOCUS       AP024920                4096 bp    DNA     circular BCT 01-OCT-2021
DEFINITION  Klebsiella aerogenes SL269 plasmid pSL269small DNA, complete
            sequence.
ACCESSION   AP024920
VERSION     AP024920.1
DBLINK      BioProject: PRJDB11945
            BioSample: SAMD00389787
KEYWORDS    .
SOURCE      Klebsiella aerogenes
  ORGANISM  Klebsiella aerogenes
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Mori,N., Tada,T. and Uehara,Y.
  TITLE     A transferrable IncL/M plasmid harboring a gene encoding IMP-1
            metallo-beta-lactamase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Uehara,Y. and Tada,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2021) Contact:Yuki Uehara Juntendo University;
            Bunkyo-ku, Hongo 2-1-1, Tokyo 113-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Genome Coverage       :: 90x
            Sequencing Technology :: Oxford nanopore MinION; Illumina Miseq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Klebsiella aerogenes"
                     /mol_type="genomic DNA"
                     /strain="SL269"
                     /host="Homo sapiens"
                     /db_xref="taxon:548"
                     /plasmid="pSL269small"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-23"
     gene            complement(158..502)
                     /locus_tag="SL269_47930"
     CDS             complement(158..502)
                     /locus_tag="SL269_47930"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ65009.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="hypothetical protein"
     gene            1866..2099
                     /locus_tag="SL269_47940"
     CDS             1866..2099
                     /locus_tag="SL269_47940"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_013095533.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ65010.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="hypothetical protein"
     gene            2121..2348
                     /gene="ygdI"
                     /locus_tag="SL269_47950"
     CDS             2121..2348
                     /gene="ygdI"
                     /locus_tag="SL269_47950"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:P65292"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipoprotein YgdI"
                     /protein_id="BCZ65011.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="ygdI"
     gene            complement(2765..2992)
                     /locus_tag="SL269_47960"
     CDS             complement(2765..2992)
                     /locus_tag="SL269_47960"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ65012.1"
                     /translation="MADDVWSYATGERSAERARHGLEHAGAEFKRAAAPVVVRLNDIEA
                     HREQERAAQHQKALELERSQRQQEYDGPSL"
                     /name="hypothetical protein"
     gene            complement(2878..3087)
                     /locus_tag="SL269_47970"
     CDS             complement(2878..3087)
                     /locus_tag="SL269_47970"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ65013.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="hypothetical protein"
     gene            complement(3091..3576)
                     /locus_tag="SL269_47980"
     CDS             complement(3091..3576)
                     /locus_tag="SL269_47980"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ65014.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="hypothetical protein"
     Typing          complement(590..668)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          744..1232
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(162..481)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.196"
                     /coverage="100.0"
                     /evalue="2.88e-62"
     Typing          complement(2769..4096)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.323"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cccggaggtg tactttttgg cgtagggcga ggcgtcgata agctcccgga cttcctccgg
       61 cttcccctga agaacgctgg caccgtcgcg ctggcggtct ttccccagca gataatcaac
      121 cgggccggca ccgcctccgc gtccccgggg atgaaactta acgatcatcg tcagccccct
      181 tttccagtac ggcatgccgc agccgctcga gtccggcatc gatggccatc agcgcggcga
      241 cgacctgcac gcggtcgaga ccgctactgc caccggcgtt aacctggcgg gcaatctggt
      301 tgaggttgtt ccccatgccg gcaagctgac gaagcagcgc cggcgagatc gagggaagtt
      361 tgccggcccg ggcgggcttc tcgtccaggc acgtctgccg catccaggcc gcgagctgtt
      421 taccgtcgca gcgttccagc agacggcggt gctcatcttc tgttacccac atcgtgagca
      481 ttttgctgcg tttgtctgcc aatccggcct cctgataacg ccgggacggg cgggaaatct
      541 tttcccggtc ggcgcggtgt tgggcaagcc gcaggcgcgt cagtggcatt tagcgggttt
      601 cggggcgcag ccctgaacca gtcacgtagc gctagcggag tgtatactgg cttactatgg
      661 tggcacgacg ggaatcggga tgaaatagtc agtcatgaaa actgtgagat gaggctcagg
      721 tgcgtaagag gctgatgcct gtcccggtgg atattccgct tcctcgctca ctgactcgct
      781 acgctcggtc gttcggctgc ggcgagcggt acggactcac ggataaagac gtggcgggga
      841 aatacgtgaa tgcaggaggg gaagaaagga tgcgccgctg ccggttttcc ataggctccg
      901 cccccctgac aagcatcaca gaatctgacg ctcaaatcag tggtggcgaa acccgatgcg
      961 gcttaaagat accaggcgtt tccccctgat ggctccctcg tgctcccccg cattcctgcc
     1021 tttcggttta ccggtgtcat tccgctgtta tggccgcggt ttctcattcc acgcctgaca
     1081 ctcagttccg cgaaggcagt tcgctccagg attgactgta tgcacgaacc ccccgttcag
     1141 tccgactgct gcgccttatc cggtaactat cgtcttgagt ccaacccgga aagacacgaa
     1201 aaatcgctac tggcagcagc cattagtaac tggaggtata cgagagacaa attaagaggt
     1261 cttgaagtat gggcctgaga gaggctacac tgaaagaaca gttttggttt ctgcggtctc
     1321 gtatacccag ttacctggtt aagcagttcc ccaactgact taaccttcga tcaaaccacc
     1381 tccccaggtg gttttttcgt tttcagagca agagattacg actgtaacga caggatcaca
     1441 agaagatcat ttactcagag agactaacta ccactatgtt tgcctatcaa catacttagg
     1501 aaacattact tgagttaacg actccacctt acttttagta tctcgcaatg aatcttccga
     1561 ccgccgttgt gttgaagttc tattgcgcca ccggggtata aatcactttc gtttaactca
     1621 attaaaagct cgttcttgtg agcatcctct aaataaaggt aataacaccc accataaagg
     1681 ttctgcgtat ctggcacggc aacaaaagat ttctttccga acagtaaaac agcctctttt
     1741 tctccatgct ctgtcagcaa taatcgaaat tcttgtccat tcaatcctct tttcgcatga
     1801 aaaaacgacc tatacttaga tggttagtga ccatatccac tttaccttta agcagggaac
     1861 ctattatgaa taaaaaaacg ctgttggcgg ccatgatggt cgcgttggtg acctttgccg
     1921 ctgctggatg ttcttcaaac caagaaataa aaaccacaga tgatcgcact atcgttacag
     1981 acggcaaacc caaagttgac agtgatacgg gattagtatc ctacaaagat gcccatacag
     2041 gccgaaccga acagatcaac cgtgacaaaa ttaccaacat gagtgaacta gacaactgat
     2101 acggataaaa ggagctggct ttgaaaaaga tttttgtttc agtgttcgca gcggcagttg
     2161 cgctttcagc cctaaccggc tgcacgcgca caagctatgc catccacacc aacgacgggc
     2221 gtacaattgt cagcgacggc aagcctacag aatcagactc aggcctgctg ggctataaag
     2281 acgcaaatgg tgtcaaacag cagatcaata aagccgacgt taaagaagtc tctgaaattc
     2341 cgcattgagg catcgctctc tccgaaaagg gccgctaatg cggccctttt ctttatctgc
     2401 cccaacaaca ggcaaaaaaa gcccccgctg ggggccaaat acctgcagtg gctggatagt
     2461 actgcattcc tgatgaagga gacgaataag tatgggccgg atttttgcgt ctgcaggctc
     2521 ggtcgccaca atttttccgt acccgccagg catcatgctt ttaccataac atcccttgcc
     2581 cgcacctctc cggacgggtt cagcgcgaat gcggcgtcag tcctgcaaaa ccgacagtaa
     2641 accaccccgc aaaccgtgca ttctgcgcct gataaatctc acccgactgc atagccatgc
     2701 agccgggtga atggcaccgg gtttcggcgc agatcctcaa tgaaagcccc gttttatccg
     2761 gttatcacag cgacggaccg tcatactcct gctgacgctg cgatcgttcc agctccagcg
     2821 ctttctggtg ctgcgctgcg cgctcctgct cccggtgtgc ctcgatgtcg ttcagcctta
     2881 cgacaaccgg cgcagctgct cgcttaaact cagcacctgc gtgctcaagt ccgtgacgcg
     2941 ctcgctcagc gctgcgttct cccgttgcat aagaccacac atcgtccgcc attccgcgaa
     3001 ggcgctctcc cactcgtcca gccttttcga gtagtcctgc tgtagctgct ctaatgcgct
     3061 cagcaactgt ttttccagtt ccgtcatgtg ctatttcccc gccagtatca tccagctcga
     3121 atcctctccg aaccgtcccg cgtccgggtt caccctcacg cagcggcgtc gttcctctcc
     3181 gcaggtcgtg agctgcacgc cgctgttcct ctcggacagc atggcctgcg tgctcttctg
     3241 ctcccggatg gtcgtgtaat tgtcgagtat ctgctgcccc tgccaccata gaatgcccgc
     3301 acttgaggct atcagcagca cggagaccag gacgatggtc agccacgtct ggctgaccat
     3361 gcgcagcatc cctttcgtac gctggctcat ggctgaggac agcatccggt cgtggtcgtg
     3421 gatggcggcg ctgattctct tctcgctttc gttcagttcc gctctgacag acttctcgtg
     3481 ctcgctgaat gcggctttca gcatctcgcc ggtagtctgc tgctgcactt tcgatttctg
     3541 ctctaagtcc ttcgccagcg ttaaaagact gttcatagat ggctcccttc agtcggatgt
     3601 ttcgcccccc gtccgggtcg gcaatgctga tgctgctttt tgtggtgcga accacctcaa
     3661 aacctgcgct ttccagcgcc tcagtgacgt cctgacgcgt tttcagctcc ccggacgagg
     3721 ctaaggccag taaaccccgc gtaatcgcct gagcggcttc ctgcttcgtt tccggcagcg
     3781 cggacggcgt gaccagtgcc cgccggtttt ccggcgcgtt cgggtcgtgc agccccagac
     3841 tgccgttcac gatggtctgc caggcatcaa tgcgaggacg gtcggcgcgg tcatagtatg
     3901 gctggagccg tttgccggtc agcagctccg tgttcgggat caggaagttc agctccagcc
     3961 gccccttgtc ccggtgctca acccacagca cgctgtactg gtctttatcg agtccgggca
     4021 tcagaacccg ctcgaagctc gccatcagcg tttcacgctg cccgggcggt aagtcctgtt
     4081 cggcaaagga cagcac
//
