LOCUS       AP024915                4096 bp    DNA     circular BCT 01-OCT-2021
DEFINITION  Enterobacter cloacae SL264 plasmid pSL264small DNA, complete
            sequence.
ACCESSION   AP024915
VERSION     AP024915.1
DBLINK      BioProject: PRJDB11945
            BioSample: SAMD00389785
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Mori,N., Tada,T. and Uehara,Y.
  TITLE     A transferrable IncL/M plasmid harboring a gene encoding IMP-1
            metallo-beta-lactamase
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Uehara,Y. and Tada,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2021) Contact:Yuki Uehara Juntendo University;
            Bunkyo-ku, Hongo 2-1-1, Tokyo 113-8540, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.7
            Genome Coverage       :: 104x
            Sequencing Technology :: Oxford nanopore MinION; Illumina Miseq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST https://dfast.ddbj.nig.ac.jp/
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="SL264"
                     /host="Homo sapiens"
                     /db_xref="taxon:550"
                     /plasmid="pSL264small"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-04-25"
     gene            complement(475..819)
                     /locus_tag="SL264_47850"
     CDS             complement(475..819)
                     /locus_tag="SL264_47850"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ55379.1"
                     /translation="MADKRSKMLTMWVTEDEHRRLLERCDGKQLAAWMRQTCLDEKPAR
                     AGKLPSISPALLRQLAGMGNNLNQIARQVNAGGSSGLDRVQVVAALMAIDAGLERLRHA
                     VLEKGADDDR"
                     /name="hypothetical protein"
     gene            2183..2416
                     /locus_tag="SL264_47860"
     CDS             2183..2416
                     /locus_tag="SL264_47860"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_013095533.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ55380.1"
                     /translation="MNKKTLLAAMMVALVTFAAAGCSSNQEIKTTDDRTIVTDGKPKVD
                     SDTGLVSYKDAHTGRTEQINRDKITNMSELDN"
                     /name="hypothetical protein"
     gene            2438..2665
                     /gene="ygdI"
                     /locus_tag="SL264_47870"
     CDS             2438..2665
                     /gene="ygdI"
                     /locus_tag="SL264_47870"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:P65292"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipoprotein YgdI"
                     /protein_id="BCZ55381.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPTES
                     DSGLLGYKDANGVKQQINKADVKEVSEIPH"
                     /name="ygdI"
     gene            complement(3195..3404)
                     /locus_tag="SL264_47880"
     CDS             complement(3195..3404)
                     /locus_tag="SL264_47880"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ55382.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMCGLMQRENA
                     ALSERVTDLSTQVLSLSEQLRRLS"
                     /name="hypothetical protein"
     gene            complement(3408..3893)
                     /locus_tag="SL264_47890"
     CDS             complement(3408..3893)
                     /locus_tag="SL264_47890"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCZ55383.1"
                     /translation="MNSLLTLAKDLEQKSKVQQQTTGEMLKAAFSEHEKSVRAELNESE
                     KRISAAIHDHDRMLSSAMSQRTKGMLRMVSQTWLTIVLVSVLLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLTTCGEERRRCVRVNPDAGRFGEDSSWMILAGK"
                     /name="hypothetical protein"
     Typing          complement(907..985)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.8899999999999995e-34"
     Typing          1061..1549
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.747"
                     /coverage="94.0"
                     /evalue="7.82e-151"
     Typing          complement(4..485)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="97.516"
                     /coverage="99.0"
                     /evalue="6.45e-99"
     Typing          complement(3086..4095)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.703"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 gcggacggcg tgaccagtgc ccgccggttt tccggcgcgt tcgggtcgtg cagccccaga
       61 ctgccgttca cgatggtctg ccaggcatca atgcgaggac ggtcggcgcg gtcatagtat
      121 ggctggagcc gtttgccggt cagcagctcc gtgttcggga tcaggaagtt cagctccagc
      181 cgccccttgt cccggtgctc aacccacagc acgctgtact ggtctttatc gagtccgggc
      241 atcagaaccc gctcgaagct cgccatcagc gtttcacgct gcccgggcgg taagtcctgt
      301 tcggcaaagg acagcacccc ggaggtgtac tttttggcgt agggcgaggc gtcgataagc
      361 tcccggactt cctccggctt cccctgaaga acgctggcac cgtcgcgctg gcggtctttc
      421 cccagcagat aatcaaccgg gccggcaccg cctccgcgtc cccggggatg aaacttaacg
      481 atcatcgtca gccccctttt ccagtacggc atgccgcagc cgctcgagtc cggcatcgat
      541 ggccatcagc gcggcgacga cctgcacgcg gtcgagaccg ctactgccac cggcgttaac
      601 ctggcgggca atctggttga ggttgttccc catgccggca agctgacgaa gcagcgccgg
      661 cgagatcgag ggaagtttgc cggcccgggc gggcttctcg tccaggcacg tctgccgcat
      721 ccaggccgcg agctgtttac cgtcgcagcg ttccagcaga cggcggtgct catcttctgt
      781 tacccacatc gtgagcattt tgctgcgttt gtctgccaat ccggcctcct gataacgccg
      841 ggacgggcgg gaaatctttt cccggtcggc gcggtgttgg gcaagccgca ggcgcgtcag
      901 tggcatttag cgggtttcgg ggcgcagccc tgaaccagtc acgtagcgct agcggagtgt
      961 atactggctt actatggtgg cacgacggga atcgggatga aatagtcagt catgaaaact
     1021 gtgagatgag gctcaggtgc gtaagaggct gatgcctgtc ccggtggata ttccgcttcc
     1081 tcgctcactg actcgctacg ctcggtcgtt cggctgcggc gagcggtacg gactcacgga
     1141 taaagacgtg gcggggaaat acgtgaatgc aggaggggaa gaaaggatgc gccgctgccg
     1201 gttttccata ggctccgccc ccctgacaag catcacagaa tctgacgctc aaatcagtgg
     1261 tggcgaaacc cgatgcggct taaagatacc aggcgtttcc ccctgatggc tccctcgtgc
     1321 tcccccgcat tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcggtttc
     1381 tcattccacg cctgacactc agttccgcga aggcagttcg ctccaggatt gactgtatgc
     1441 acgaaccccc cgttcagtcc gactgctgcg ccttatccgg taactatcgt cttgagtcca
     1501 acccggaaag acacgaaaaa tcgctactgg cagcagccat tagtaactgg aggtatacga
     1561 gagacaaatt aagaggtctt gaagtatggg cctgagagag gctacactga aagaacagtt
     1621 ttggtttctg cggtctcgta tacccagtta cctggttaag cagttcccca actgacttaa
     1681 ccttcgatca aaccacctcc ccaggtggtt ttttcgtttt cagagcaaga gattacgact
     1741 gtaacgacag gatcacaaga agatcattta ctcagagaga ctaactacca ctatgtttgc
     1801 ctatcaacat acttaggaaa cattacttga gttaacgact ccaccttact tttagtatct
     1861 cgcaatgaat cttccgaccg ccgttgtgtt gaagttctat tgcgccaccg gggtataaat
     1921 cactttcgtt taactcaatt aaaagctcgt tcttgtgagc atcctctaaa taaaggtaat
     1981 aacacccacc ataaaggttc tgcgtatctg gcacggcaac aaaagatttc tttccgaaca
     2041 gtaaaacagc ctctttttct ccatgctctg tcagcaataa tcgaaattct tgtccattca
     2101 atcctctttt cgcatgaaaa aacgacctat acttagatgg ttagtgacca tatccacttt
     2161 acctttaagc agggaaccta ttatgaataa aaaaacgctg ttggcggcca tgatggtcgc
     2221 gttggtgacc tttgccgctg ctggatgttc ttcaaaccaa gaaataaaaa ccacagatga
     2281 tcgcactatc gttacagacg gcaaacccaa agttgacagt gatacgggat tagtatccta
     2341 caaagatgcc catacaggcc gaaccgaaca gatcaaccgt gacaaaatta ccaacatgag
     2401 tgaactagac aactgatacg gataaaagga gctggctttg aaaaagattt ttgtttcagt
     2461 gttcgcagcg gcagttgcgc tttcagccct aaccggctgc acgcgcacaa gctatgccat
     2521 ccacaccaac gacgggcgta caattgtcag cgacggcaag cctacagaat cagactcagg
     2581 cctgctgggc tataaagacg caaatggtgt caaacagcag atcaataaag ccgacgttaa
     2641 agaagtctct gaaattccgc attgaggcat cgctctctcc gaaaagggcc gctaatgcgg
     2701 cccttttctt tatctgcccc aacaacaggc aaaaaaagcc cccgctgggg gccaaatacc
     2761 tgcagtggct ggatagtact gcattcctga tgaaggagac gaataagtat gggccggatt
     2821 tttgcgtctg caggctcggt cgccacaatt tttccgtacc cgccaggcat catgctttta
     2881 ccataacatc ccttgcccgc acctctccgg acgggttcag cgcgaatgcg gcgtcagtcc
     2941 tgcaaaaccg acagtaaacc accccgcaaa ccgtgcattc tgcgcctgat aaatctcacc
     3001 cgactgcata gccatgcagc cgggtgaatg gcaccgggtt tcggcgcaga tcctcaatga
     3061 aagccccgtt ttatccggtt atcacagcga cggaccgtca tactcctgct gacgctgcga
     3121 tcgttccagc tccagcgctt tctggtgctg cgctgcgcgc tcctgctccc ggtgtgcctc
     3181 gatgtcgttc agccttacga caaccggcgc agctgctcgc ttaaactcag cacctgcgtg
     3241 ctcaagtccg tgacgcgctc gctcagcgct gcgttctccc gttgcataag accacacatc
     3301 gtccgccatt ccgcgaaggc gctctcccac tcgtccagcc ttttcgagta gtcctgctgt
     3361 agctgctcta atgcgctcag caactgtttt tccagttccg tcatgtgcta tttccccgcc
     3421 agtatcatcc agctcgaatc ctctccgaac cgtcccgcgt ccgggttcac cctcacgcag
     3481 cggcgtcgtt cctctccgca ggtcgtgagc tgcacgccgc tgttcctctc ggacagcatg
     3541 gcctgcgtgc tcttctgctc ccggatggtc gtgtaattgt cgagtatctg ctgcccctgc
     3601 caccatagaa tgcccgcact tgaggctatc agcagcacgg agaccaggac gatggtcagc
     3661 cacgtctggc tgaccatgcg cagcatccct ttcgtacgct ggctcatggc tgaggacagc
     3721 atccggtcgt ggtcgtggat ggcggcgctg attctcttct cgctttcgtt cagttccgct
     3781 ctgacagact tctcgtgctc gctgaatgcg gctttcagca tctcgccggt agtctgctgc
     3841 tgcactttcg atttctgctc taagtccttc gccagcgtta aaagactgtt catagatggc
     3901 tcccttcagt cggatgtttc gccccccgtc cgggtcggca atgctgatgc tgctttttgt
     3961 ggtgcgaacc acctcaaaac ctgcgctttc cagcgcctca gtgacgtcct gacgcgtttt
     4021 cagctccccg gacgaggcta aggccagtaa accccgcgta atcgcctgag cggcttcctg
     4081 cttcgtttcc ggcagc
//
