LOCUS       AP024697                5593 bp    DNA     circular BCT 01-SEP-2021
DEFINITION  Escherichia coli FUJ80155 plasmid pFUJ80155-3 DNA, complete
            sequence.
ACCESSION   AP024697
VERSION     AP024697.1
DBLINK      BioProject: PRJDB11569
            BioSample: SAMD00317523
KEYWORDS    .
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1
  AUTHORS   Suzuki,M., Harada,Y. and Doi,Y.
  TITLE     Complete sequence of Escherichia coli strain FUJ80154
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5593)
  AUTHORS   Suzuki,M., Harada,Y. and Doi,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAY-2021) Contact:Masahiro Suzuki Fujita Health
            University, School of Medicine, Microbiology; Dengakugakubo 1-98,
            Kutsukake-cho, Toyoake, Aichi 470-1192, Japan URL
            :http://www.fujita-hu.ac.jp/~microb/index.html
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Unicycler v. 0.4.9b
            Genome Coverage       :: 106x
            Sequencing Technology :: MiSeq; MinION
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.13 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..5593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="FUJ80155"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pFUJ80155-3"
                     /geo_loc_name="Japan:Aichi"
                     /collection_date="2019-09-25"
     gene            complement(99..1079)
                     /locus_tag="FJMB80155_p310"
     CDS             complement(99..1079)
                     /locus_tag="FJMB80155_p310"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:Q50290"
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenine-specific methylase"
                     /protein_id="BCX41806.1"
                     /translation="MARNATNKLLHKAKKSKSDEFYTQYCDIENELQYYREHFSDKVVY
                     CNCDDPRVSNFFKYFAVNFDNLGLKKLIASCYVENKEGFSSSEAAKNGFYYEYHKENGK
                     KLVFDDISVSSFCGDGDFRSSESIDLLKKSDVVVTNPPFSLFREYLDQLIKYDKKFLII
                     ANVNSITYKEVFNLIKENKIWLGVHLGRGVSGFIVPEHYELYGTEARIDSNGNRIISPN
                     NCLWLTNLDVFIRHKDLPLTRKYFGNESSYPKYDNYDAINVNKTKDIPLDYNGVMGVPI
                     TFLHKFNPEQFELIKFRKGVDEKDLSINGKCPYFRILIKNKRLQK"
                     /name="putative adenine-specific methylase"
     gene            complement(1109..1942)
                     /locus_tag="FJMB80155_p320"
     CDS             complement(1109..1942)
                     /locus_tag="FJMB80155_p320"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCX41807.1"
                     /translation="MSNKKQSNRLTEQHKLSQGVIGIFGDYAKAHDLAVGEVSKLVKKA
                     LSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTLFVSNSSIKPDGGIVEVKDDYGEW
                     RVVLVAEAKHQGKDIINIRNGLLVGKRGDQDLMAAGNAIERSHKNISEIANFMLSESHF
                     PYVLFLEGSNFLTENISITRPDGRVVNLEYNSGILNRLDRLTAANYGMPINSNLCINKF
                     VNHKDKSIMLQAASIYTQGDGREWDSKIMFEIMFDISTTSLRVLGRDLFEQLTSK"
                     /name="hypothetical protein"
     gene            complement(2331..2528)
                     /locus_tag="FJMB80155_p330"
     CDS             complement(2331..2528)
                     /locus_tag="FJMB80155_p330"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCX41808.1"
                     /translation="MSYPWSVSASWKYRNGHWMALRWDGNQRQAKMLSARFRCIFWDNG
                     WFGGTGNALQSDLAENTGTR"
                     /name="hypothetical protein"
     gene            complement(2559..2792)
                     /locus_tag="FJMB80155_p340"
     CDS             complement(2559..2792)
                     /locus_tag="FJMB80155_p340"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCX41809.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQQDNA
                     MLNEQVMQLSQQVQHLSERTERLSQLYSENRR"
                     /name="hypothetical protein"
     gene            complement(2799..3317)
                     /locus_tag="FJMB80155_p350"
     CDS             complement(2799..3317)
                     /locus_tag="FJMB80155_p350"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCX41810.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETMLNDEFSKLEKSVSKAVTSNE
                     TKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTVSG
                     GTLWYLGGMIQANLEEIRIQEETLQKLNAKTWGVEFVQDGRRKFLVIPQGKSATVIPYQ
                     GKDWVQLTE"
                     /name="hypothetical protein"
     gene            complement(3995..4240)
                     /locus_tag="FJMB80155_p360"
     CDS             complement(3995..4240)
                     /locus_tag="FJMB80155_p360"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCX41811.1"
                     /translation="MRRVCLGEPVARSGKLPTLAPPLLRQLAAIGNNLNQTARKVNSEQ
                     WSSGDRVQVVAALMAIERELRSLRQVVREHGARDDS"
                     /name="hypothetical protein"
     gene            4382..4573
                     /gene="rop"
                     /locus_tag="FJMB80155_p370"
     CDS             4382..4573
                     /gene="rop"
                     /locus_tag="FJMB80155_p370"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_002213433.1"
                     /codon_start=1
                     /transl_table=11
                     /product="rop protein"
                     /protein_id="BCX41812.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
                     /name="rop"
     gene            complement(4596..4841)
                     /locus_tag="FJMB80155_p380"
     CDS             complement(4596..4841)
                     /locus_tag="FJMB80155_p380"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCX41813.1"
                     /translation="MSEEAEERRKLHFLLTHLCGISHPAWRTFHTIRTDAAWLSQYTLR
                     YRYVTGSGLRPDTRKVNGLTRPDGLVCSRHPLTDKL"
                     /name="hypothetical protein"
     Typing          complement(4666..4749)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="99.0"
                     /evalue="1.09e-36"
     Typing          4760..5316
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.464"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(2456..4005)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.979"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accttcgaaa aaaccaccag gagaggtggt tttttcgttt ctggtaccga agcttacgac
       61 gaagctaatg atctcaagaa aaaactaaca aacatcaatt acttttgtaa tcgtttgttt
      121 tttatcaaaa ttctgaaata agggcattta ccatttatag acaaatcttt ttcatcaaca
      181 ccctttctaa attttattaa ctcaaattgc tcagggttaa acttatgcaa gaatgtgata
      241 ggaaccccca taaccccatt gtaatctaat ggaatatcct ttgttttgtt tacatttata
      301 gcatcataat tatcatattt tggatatgaa ctttcattcc caaaatattt tcttgtaaga
      361 ggcaagtctt tatgcctaat aaagacatct aggttagtta gccataagca gttgtttggc
      421 gagataattc tattaccatt agaatcaatt ctcgcctcag taccatataa ttcataatgc
      481 tctggaacaa taaatccaga aacacctctc ccgagatgaa ccccaagcca aatcttattt
      541 tccttaatta gattaaacac ctctttatat gttattgaat taacatttgc aattataagg
      601 aatttcttat catacttaat tagttgatca agatactctc taaataacga gaatggagga
      661 ttcgtaacaa caacatctga tttttttagc agatcaatgc tctccgaact gcgaaaatcg
      721 ccatcgccac agaaagaaga aacactaata tcatcaaaaa ctaatttctt tccattttct
      781 ttatgatatt catagtaaaa tccgttcttc gcggcttcgc tactagaaaa accttcttta
      841 ttctctacat agcaagatgc tattaacttt ttcaagccaa gattatcaaa attcactgca
      901 aaatatttaa agaaattgct tactctagga tcatcacaat tgcaataaac aaccttatca
      961 gagaagtgct ctctgtagta ttgcagttcg ttctcaatat cacaatactg agtgtaaaat
     1021 tcgtcgcttt tcgatttttt agctttgtgc agtaacttgt ttgttgcatt tctagccatt
     1081 aattaaaacc ttattaaaat aaaaaatatc acttagatgt aagctgttca aacaagtcac
     1141 gccccaacac tctgagcgaa gtcgttgata tatcaaacat tatttcaaac ataattttcg
     1201 aatcccactc cctcccatct ccttgagtat atatagatgc tgcttgtagc ataatgcttt
     1261 tgtctttatg atttacaaat ttgttaatac atagattact atttataggc attccataat
     1321 tagctgcagt tagtcgatct aacctattta atataccaga attatactca agattaacaa
     1381 cccttccatc tggtcttgtt attgagatat tttctgttaa aaagttagac ccctctaaga
     1441 aaaggacgta aggaaagtgg ctctcagaga gcataaaatt cgctatctct gatatattct
     1501 tatgagatct ttcgatagca ttaccagcag ccattaaatc ttgatctcct cttttcccaa
     1561 ctaacaaacc attccttata tttataatat ctttaccttg gtgtttggct tcagcaacaa
     1621 gtacaactct ccattcacca taatcatctt tgacctctac aattccacca tcaggtttga
     1681 tgctggaatt tgaaacaaat aaagtaccgc caagatcagg gtcaattttt tttaaagctt
     1741 catttatttc tgttttcttt atactatctc tatatcgaaa tgataattga gggtattcgt
     1801 tgctaagagc tttctttact aattttgaaa cctcaccaac agcgagatca tgagcttttg
     1861 cataatcccc aaaaatccca attacacctt gagataactt atgttgttca gttagcctat
     1921 ttgactgttt tttattagac atgaatccat gtttccactg ttattttata aagttacata
     1981 tgtcgataga ttaacatacg tttgatttac cataatccct tattgtacgc accgctgaaa
     2041 cgcgttcggc gcgatcacgg cagcagacag gtaaaaatgg caacaaacca cccgaaaaac
     2101 tgccgcgatc gcacccggta aattttaacc acatgcatag ctatgcagcc atgtgaatca
     2161 cgcaggaatt gccagccaga gacagctgaa acggattttt ttcatgcttt cggaagcgaa
     2221 gaagacggga ccggagcggg aaaacagaag ttcaggggaa tgagcgcgat ctggcaatag
     2281 aggcaaaaca cagcaacaaa agacacacca gaatcgcgcc cgtatgcgtt ttaacgcgtt
     2341 cctgtgtttt cagcaaggtc tgactgaagc gcgttcccgg tgcccccgaa ccaaccatta
     2401 tcccaaaaaa tgcaccggaa acgcgctgac aacattttag cctgtctctg attaccatcc
     2461 cagcgaaggg ccatccagtg tccgttcctg tatttccagc tggcgctcac gctccaggga
     2521 taactcatgt tcgcgctcct gtatcagccg ttcgtccctt atctcctgtt ctcgctgtat
     2581 aactggctca agcgttctgt ccgctcgctc aagtgctgca cctgctgact caactgcatg
     2641 acctgctcgt tcagcatcgc gttgtcctgt tgcgtaagcg aaaacatttt ctgcaattcc
     2701 acgaaggcgc tctcccattc gttcagccgc tgcatatagt cctgttgcag ttgctctaag
     2761 gcgttcagca aatgtctttc cagctccgtc actctgtgtc actccgtcag ttgtacccag
     2821 tccttcccct gatagggaat caccgttgcc gatttcccct gcgggatgac cagaaatttc
     2881 cttctcccgt cctgcacaaa ctccacgccc catgtcttcg cgttcagttt ctgcaatgtt
     2941 tcttcctgta tcctgatttc ttccaggttc gcctgtatca tcccgccaag ataccagagc
     3001 gtcccgccac tcacggtaaa caggaaaatg accatcccca gtaacatcat gcccgtattc
     3061 cctgccagct ttaacacgtc cttcctgtgc tgcatcatcg cctctttcac cccttcccgg
     3121 tgttttttca gtgattcctc tgtcgaagct gtgaacaggg ctatagcgtc tttgattttc
     3181 gtctcgtttg atgtcacagc cttgcttaca gatttttcga gcttgctgaa ctcgtcgttc
     3241 agcattgtct ctgtagattc ggctctctct ttcagctttt tctcgaactc cgcgcccgtc
     3301 tgtaaaagat tgctcataaa atgctccttt cagcctgata ttcttcccac cgttcgggtc
     3361 tgcaatgctg atactgcttc gcgtcaccct gaccacttca agccctgcct ctgtgagcgc
     3421 ctgaatcaca tcctgacggc cttttatctc cccgacatga taaagagcgt ctatcccgcg
     3481 tgtgacgctc tctgcaagcg cctgtttcgt ttccggcagg ttatcaggaa gggtcagcgt
     3541 ccggcggttc tccggggcgt tggggtcatg cagcccgtaa tggtgattca ccagcgtctg
     3601 ccaggcatca attctcggcc tgtctgcccg gtcgtagtac ggctggaggc gttttccggt
     3661 ctgtagctcc atgttcggaa tgacaaaatt cagctcaagc cgtcccttgt cctggtgctc
     3721 tacccacagg atgctgtact gattcttttc gagaccgggc atcagtacac gctcaaagct
     3781 cgccatcacc ttttcacgtc ctcccggcgg cagctccttc tccgcgaacg acagaacacc
     3841 tgacgtgtat ttcttcgcaa atggcgtggc atcgatgagt tcccggactt cttccggatc
     3901 accctgaagc accgttgccc cttcccggtt acgctccctt cccagcaggt aatcaaccgg
     3961 accactgcca ccgccttttc ccctggcatg aaacttaact atcatcccgc gctccatgtt
     4021 ccctgacgac ctgccgtaag ctgcgcaact ctctctcgat ggccatcagt gcggccacca
     4081 cctgaacccg gtcaccggaa gaccactgct cgctgttcac cttcctcgcc gtctgattca
     4141 ggttattccc gatggcggcc agctgacgca gtaacggcgg tgccagtgtc ggcagcttcc
     4201 cggaacgggc aaccggctcc cccaggcaga cccgccgcat ccataccgcc agttgtttac
     4261 cctcacagcg ttcaagtaac cgtgcatgtt catcatcagt aacccgtatc gtgagcatcc
     4321 tctcgcgttt catcggtatc attaccccat gaacagaaat cccccttaca cggaggcatc
     4381 agtgactaaa caggaaaaaa cagcccttaa catggcgcgc tttatcagaa gccagacatt
     4441 aacgctgctg gaaaagctca acgaactgga cgcggatgaa caggcagaca tctgtgaatc
     4501 gcttcacgac cacgccgatg agctttaccg cagctgcctc gcacgcttcg gggatgacgg
     4561 tgaaaacctc tgacacatac agctcccgga gacggtcaca gcttgtctgt gagcggatgc
     4621 cgggagcaga caagcccgtc agggcgcgtc aggccgttga ccttgcgggt gtcggggcga
     4681 agccctgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacca tgcggcatca
     4741 gtgcggattg tatgaaaagt gcgccatgcc gggtgtgaaa tgccgcacag atgtgtaagg
     4801 agaaaatgca acttccgacg ctcttccgct tcctcgctca ctgactcgct acgctcggtc
     4861 gttcggctgc ggcgagcggt atcagctcac tcaaagacgg taatgcggtt atccacagaa
     4921 tcaggggata acaccaaaag aaacatgtga gcaaaaaaca agaacccaga gaaggccacg
     4981 ccgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5041 agtcagaggt ggcgaaaccc gacaggactt aaagatacca ggcgtttccc cctggtggct
     5101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5161 cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcagt tcggtgtagg
     5221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     5281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttaacg ccactggcag
     5341 cagccactgg taactggaca aggtggattg agatattaag agttcttgaa gtggtggcct
     5401 aactgcggct acactagaag gacagtattt ggtatctgcg ctccaccaag ccagttacct
     5461 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgttggt agcggtggtt
     5521 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     5581 tcttttctac tga
//
