LOCUS       AP024492               13360 bp    DNA     circular BCT 22-FEB-2024
DEFINITION  Providencia vermicola strain N3 plasmid pPRVEN3-2 DNA, complete
            genome.
ACCESSION   AP024492
VERSION     AP024492.1
DBLINK      BioProject: PRJDB11239
            BioSample: SAMD00281820
            Sequence Read Archive: DRR274671, DRR274672
KEYWORDS    .
SOURCE      Providencia vermicola
  ORGANISM  Providencia vermicola
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Morganellaceae; Providencia.
REFERENCE   1
  AUTHORS   Sekizuka,T., Haque,A., Matsumoto,T., Sah,R. and Kuroda,M.
  TITLE     complete genome sequences of blaNDM-1 and blaOXA-181 co-harboring
            pan-drug resistant Providencia vermicola str. N3
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13360)
  AUTHORS   Sekizuka,T., Haque,A., Matsumoto,T., Sah,R. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-FEB-2021) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: Canu v. 1.4; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 342x
            Sequencing Technology :: PacBio RS II; NextSeq500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..13360
                     /organism="Providencia vermicola"
                     /mol_type="genomic DNA"
                     /strain="N3"
                     /host="Homo sapiens"
                     /db_xref="taxon:333965"
                     /plasmid="pPRVEN3-2"
                     /geo_loc_name="Nepal"
                     /collection_date="2015"
     gene            101..898
                     /gene="blaOXA-181"
                     /locus_tag="PRVEN3_P2010"
     ARG             102..895
                     /name="blaOXA-181"
                     /gene="blaOXA-181"
                     /gene_name="OXA-48 family carbapenem-hydrolyzing class D
                     beta-lactamase OXA-181"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1273..2895
                     /locus_tag="PRVEN3_P2020"
     CDS             1273..2895
                     /locus_tag="PRVEN3_P2020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCT08335.1"
                     /translation="MEMYQNQLDNYEIRDFDISKFMLFGNVSNHYIDQCTLTNDYDRRF
                     IYRKEIEEIKNNILSGDNVIITSDLGNGKTILVKILMSIMTRVGFNCYLYSSNEYSFTK
                     DIEILSNNKNKNIIFIDNYDNSIDELKYILEQSHENIQVVITARHYGYENTKELLLPIG
                     LNKFKNYNLDYLLDDEVDSFINIVDNLGAWGDKAGLSKKRKLHGLDDEAKKQLSILLLS
                     VLESESIKTKINEITNNIFKNKGYKETIFAILLIDIIGHNMDRSIISDMAMNNVIFSTD
                     FIENKGVINLFRIEKGLIKTRSSTLSRFLISNQFEPMYIANQLLEIVNNLNKNSSDTKD
                     SSWQKIIKSLLRFSIVEKVLPQKRSAIKFYYEKLKVIIPRLIKEPHYWVQYAMSVIPFK
                     DYPNASIYLETAYSLAELKGSEYHTNNINTQQARLFFLKGLECKGAESFDLFTKGDSLM
                     QIIPNDIFKYRQISLYITFYEKVYATFNKGQKVEYERALKKIIKDSESRDLENGIYKSG
                     DKWIEETIEKMNNILNIIENNRK"
                     /name="hypothetical protein"
     gene            complement(2960..3226)
                     /locus_tag="PRVEN3_P2030"
     CDS             complement(2960..3226)
                     /locus_tag="PRVEN3_P2030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_010971059.1"
                     /codon_start=1
                     /transl_table=11
                     /product="translation repressor RelE"
                     /protein_id="BCT08336.1"
                     /translation="MVWTINYSERALKSLRKMDKQNARRIVDFMDLRIAVSDDPRQSGK
                     PLKGELGEFWRYRVGDYRILCQIIDDELIILAATIGHRKEVYL"
                     /name="translation repressor RelE"
     gene            complement(3210..3434)
                     /locus_tag="PRVEN3_P2040"
     CDS             complement(3210..3434)
                     /locus_tag="PRVEN3_P2040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_011390943.1"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="BCT08337.1"
                     /translation="MLAIRLSDDIETRLESLAKQTGRTKTFYAREAILTHLEDLEDYYL
                     SAETVAKIRRGEELVHSSEDVRKALGLDD"
                     /name="CopG family transcriptional regulator"
     gene            3622..3750
                     /locus_tag="PRVEN3_P2050"
     CDS             3622..3750
                     /locus_tag="PRVEN3_P2050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001347255.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCT08338.1"
                     /translation="MGLKPGPKPIAKSTGKPDQRRRDNKDTPGNTPELKPSKSTGK"
                     /name="hypothetical protein"
     gene            3995..5398
                     /locus_tag="PRVEN3_P2060"
     CDS             3995..5398
                     /locus_tag="PRVEN3_P2060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000974326.1"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="BCT08339.1"
                     /translation="MSAESSGVFTLKEINRIKIIQDVIERRITTRRAAEHLGISDRQCR
                     RLLARYREGGPLGMASRRCGMRGNRQLPPGLADQALELIKTRYADFGPTLAREKLEELH
                     GLFLGKETVRRIMVRAGLWVPRKQRAARIPQPRYRRPCTGELIQIDGCDHDWFEGRGPA
                     CTALVYVDDATSKLMELLFVKSESTFSYFEATRRYIDKHGKPLALYSDKAGVFRVNNKH
                     ATGGDGHTQFGRAMHELNIQTICAETSPAKGRVERAHLTLQDRLVKELRLQGICSMEAA
                     NDFAEAYMADYNRRFGKVPRHDFDVHRAVEHDEDLGLIFTVREKRKVSKSLTIQYDKML
                     YLIEDSELSRRAIGKYIDVYHYPDGRKELRLNGTLLPYSTYDRLSEIDQGAIVDNKRLG
                     RTLEFISLVQSKRDNTRSQSIPAGDGPSRRRPKQEGKKSQRSLDNDDMLEALKQLQSRS
                     EDIFGKRAR"
                     /name="transposase"
     gene            complement(5427..6059)
                     /locus_tag="PRVEN3_P2070"
     CDS             complement(5427..6059)
                     /locus_tag="PRVEN3_P2070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCT08340.1"
                     /translation="MLRYELTPNNAGFILWGDSEALNELHELIHYIVDESPLIKVKDGF
                     MLSLAYDIRKAREGNRRVEQHQYDQHDTYKLYGVELLWPLVLVQSSILRNSMGYIQTDK
                     NQLSVMYAFEYLIESALTESERTTSNDIMLTVKYASDSDFNFIEDNIDSRCCYFISLSP
                     EQRKKQLISIVRSFHSLWGKYAREKQDIKMLNEMNNTSWVWPDNINW"
                     /name="hypothetical protein"
     gene            6179..7102
                     /locus_tag="PRVEN3_P2080"
     CDS             6179..7102
                     /locus_tag="PRVEN3_P2080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_013096391.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="BCT08341.1"
                     /translation="MAKQKFKITNWPTYNKALINRGSITFWLDDEAIQAWYESATPSSR
                     GRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLRCPDYSCVSRRAKSVN
                     VSFKTFTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRKLHLAVDSNTHEIICA
                     DLSLNNVTDSEAFPGLIRQTHRKIRAASADGAYDTRLCHDELRRKKISALIPPRKGAGY
                     WPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVKQLFGGSLTLRDYDGQ
                     VAEAMALVRALNKMTKAGMPESVRIA"
                     /name="IS5 family transposase"
     gene            complement(7382..7810)
                     /locus_tag="PRVEN3_P2090"
     CDS             complement(7382..7810)
                     /locus_tag="PRVEN3_P2090"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCT08342.1"
                     /translation="MALVSISEAARLTGKARSTLHKYIKQGRLSTTTDQNTGKKTIETS
                     ELIRVFGKISNLPTTISDSITSVSKLQPETQNDTQSLQAKLQLLEQENTHLKAEKELLS
                     KNLDDIRQAMLLIESKLPTTPEPVAPVPTKKPWQFWKK"
                     /name="hypothetical protein"
     gene            complement(7813..8511)
                     /locus_tag="PRVEN3_P2100"
     CDS             complement(7813..8511)
                     /locus_tag="PRVEN3_P2100"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCT08343.1"
                     /translation="MSELIAYKSNALIEASYKLTLQEQRLLLMCIGKLNPRDKTPQKTF
                     KITSGDFYLAFPDMGKSNAERHLKEAIDKLAERWIYIQNNNERRRIRWIQEEAEYFEGE
                     GKIEIVFADSIMPYLTQLQGQFTKIVIKNVSTLKSVYSIRLYELLMQFKVIGDRLISID
                     DFRSMLGLDEKKYATFKSLNQWVIKPAIKELNQKSNLSVTVDTVKKGRSVFALHFHFKE
                     DQQIKMMLED"
                     /name="hypothetical protein"
     gene            complement(10278..10514)
                     /locus_tag="PRVEN3_P2110"
     CDS             complement(10278..10514)
                     /locus_tag="PRVEN3_P2110"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCT08344.1"
                     /translation="MQFFTAHEVIIISAKYCDRCHLHAEKDDPEFHEFLSIDRQAGFGS
                     TFGDGNNLKLDLCQHCVKELLNPWLSVSEPDSF"
                     /name="hypothetical protein"
     gene            10804..11766
                     /locus_tag="PRVEN3_P2120"
     CDS             10804..11766
                     /locus_tag="PRVEN3_P2120"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCT08345.1"
                     /translation="MENSELMKLKSLIGTQLESDLDLDKIERLILNGNAILFVGAGFSL
                     ESINVDGYKLPLAKDLAKIISKENSRYFQSIGADENYIKQVENCDDLMISSDIFLKEVP
                     QKNNLLNILKRTFSVKEVTDEQVSICKNSWRKIYTTNYDNAIELASLKSGKTITSLDLT
                     DDTGKYRRHKDICLHINGKIDGANERDLDNKIKLTTSSYLSSDQFLNSPWYSQFKNDIE
                     HCSAIFFVGYSMYDLDVQKILFNNKEIKSKTFFITRENSSKFDNYKLSIFGNILNIGIN
                     GFSKILNELSPRFYVSTSKSIIKALINMRSFEIQLSPIG"
                     /name="hypothetical protein"
     gene            11863..13125
                     /locus_tag="PRVEN3_P2130"
     CDS             11863..13125
                     /locus_tag="PRVEN3_P2130"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000608644.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="BCT08346.1"
                     /translation="MINKIDFKAKNLTSNAGLFLLLENAKSNGIFDFIENDLVFDNDST
                     NKIKMNHIKTMLCGHFIGIDKLERLKLLQNDPLVNEFDISVKEPETVSRFLGNFNFKTT
                     QMFRDINFKVFKKLLTKSKLTSITIDIDSSVINVEGHQEGASKGYNPKKLGNRCYNIQF
                     AFCDELKAYVTGFVRSGNTYTANGAAEMIKEIVANIKSDDLEILFRMDSGYFDEKIIET
                     IESLGCKYLIKAKSYSTLTSQATNSSIVFVKGEEGRETTELYTKLVKWEKDRRFVVSRV
                     LKPEKERAQLSLLEGSEYDYFFFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGH
                     LLLKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTAR
                     YVIMKLSENYPYKGVYEKCLV"
                     /name="IS1380 family transposase"
     Typing          complement(7814..8511)
                     /name="rep_cluster_1791"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gccacaattg gtcagcagtt tgctagggaa tgagagggca gagaatgata agcttagcac
       61 ttaatttgta tggatatatt gcattaagca aggggacgtt atgcgtgtat tagccttatc
      121 ggctgtgttt ttggtggcat cgattatcgg aatgccagcg gtagcaaagg aatggcaaga
      181 aaacaaaagt tggaatgctc actttactga acataaatca cagggcgtag ttgtgctctg
      241 gaatgagaat aagcagcaag gatttaccaa taatcttaaa cgggcgaacc aagcattttt
      301 acccgcatct acctttaaaa ttcccaatag cttgatcgcc ctcgatttgg gcgtggttaa
      361 ggatgaacac caagtcttta agtgggatgg acagacgcgt gatatcgccg cttggaatcg
      421 tgaccatgac ttaattaccg cgatgaagta ctcagttgtg cctgtttatc aagaatttgc
      481 ccgccaaatt ggtgaggcac gtatgagtaa aatgctgcac gccttcgatt atggcaatga
      541 ggatatctcg ggcaatgtag acagtttttg gctcgatggt ggtattcgca tttcggctac
      601 ccagcaaatc gcttttttac gcaagctgta tcacaacaag ctgcacgttt ctgagcgtag
      661 tcagcgcatc gtgaaacaag ccatgctgac cgaagccaat ggcgactata ttattcgggc
      721 taaaacggga tactcgacta gaatcgaacc taagattggc tggtgggttg gttgggttga
      781 acttgatgat aatgtgtggt tttttgcgat gaatatggat atgcccacat cggatggttt
      841 agggctgcgc caagccatca caaaagaagt gctcaaacag gagaaaatta ttccctagaa
      901 gtggttagcg cgtatttgtg tgaaatagcc gtcatataag ctgtaaagat atatggacaa
      961 aatgctgatg ctcgatgcgc ttgtagttaa tggctcatac ttaatcggtc aggcctaatc
     1021 gacttaagct ccgcgcgtta actttcagcc tgagcattag catcacattc ctaaacgtaa
     1081 aaagcctagc caatttgcta ggcttttttg ttatctcaat ggccatggtc actcaacatg
     1141 ggggtgtgaa taaaggagtg gcgatgtcag taaaggtgaa tgccgctgcg ataaagtgcc
     1201 tagcccatca aggattgtag gctttcttgg ctatcaaatg atgggaaaaa agaagttgaa
     1261 attgttagtt ctatggaaat gtatcagaat caattagata actatgaaat aagagatttt
     1321 gatatatcga aatttatgtt atttggtaat gtaagtaacc attatattga tcagtgcact
     1381 ttgacaaatg attatgatcg tagatttatc tatagaaaag aaatcgaaga aataaaaaat
     1441 aatattttat ctggcgataa tgttatcatt acaagtgact taggtaatgg gaagacgata
     1501 cttgtaaaaa tattaatgtc aataatgaca agagtcgggt ttaattgtta tttatattca
     1561 agtaatgaat attcttttac taaagatatt gagattttat caaataataa aaataagaat
     1621 ataattttta ttgataatta cgacaattct attgatgaac tcaaatatat acttgaacag
     1681 tctcatgaaa atatacaagt tgtaattact gctcgacatt atgggtatga aaatacaaag
     1741 gagctattac ttccgatagg gctaaataaa tttaaaaact ataatttgga ttacttgtta
     1801 gatgatgagg ttgatagttt tatcaatata gttgataatt tgggagcttg gggggataag
     1861 gccggtttat ctaagaagag aaagctacac gggctagatg atgaagcaaa aaaacaattg
     1921 tcaatattat tgctttcagt attggagtca gaatcaataa aaacaaaaat aaatgagata
     1981 actaataata tatttaaaaa taaaggatat aaggaaacta tttttgcaat tttattaatt
     2041 gatattattg gtcataatat ggaccgttct ataatttcag atatggcaat gaataatgtg
     2101 attttttcaa ctgatttcat tgaaaataaa ggagttatca atttatttag aattgaaaaa
     2161 ggcttaataa aaacaaggtc aagtactcta tcaaggtttt tgatttcaaa tcaatttgag
     2221 cctatgtata tagctaatca attactggaa atagttaata atcttaataa gaacagctct
     2281 gatactaaag atagcagttg gcagaaaata ataaagtctt tattgagatt ttcaatcgtt
     2341 gaaaaggtat tgccacagaa aagaagtgca attaaatttt attatgaaaa acttaaagtt
     2401 atcattccta ggttgattaa agaacctcat tactgggttc aatatgcaat gtcagtaata
     2461 ccatttaaag attatccaaa tgcttcaatt tacttggaaa cagcatatag tttagcagag
     2521 cttaaaggta gtgaatatca cacaaataac ataaataccc agcaagcaag gttgtttttt
     2581 ttaaaagggt tagaatgtaa aggagctgaa tcatttgatc tatttactaa aggcgattcc
     2641 ttaatgcaaa taatcccaaa tgacatattt aaatataggc aaatatctct ttatattact
     2701 ttttatgaaa aggtatatgc tacctttaat aaaggacaaa aagttgaata tgaaagagca
     2761 ttaaagaaaa taataaaaga ctcggaaagt agagatcttg aaaatggtat ctataaatct
     2821 ggagataaat ggatagaaga gactatagaa aaaatgaaca atatattaaa tataatagaa
     2881 aataatagaa aataatagaa aaaaatagta tttaactaca gcgctctata gtctaaagaa
     2941 agcccgcatt gcgggctaat cataaataaa cttctttacg gtgccctata gtagctgcaa
     3001 gaatgataag ctcatcatct attatttggc atagtatccg ataatcacca actctataac
     3061 gccagaactc acctagttca cctttgagtg gtttgcctga ctgtcgagga tcatctgaaa
     3121 ctgcaatacg taaatccata aaatctacaa tacgacgcgc attttgttta tccatttttc
     3181 gtagagattt taacgcccgt tcagaataat taatcgtcca gaccaagagc tttcctcacg
     3241 tcttcagacg aatgaactaa ctcttcaccg cgacgaattt ttgcaacggt ttcagcggaa
     3301 aggtagtagt cttccaaatc ttccaaatga gtgaggatag cttctctcgc atagaatgtt
     3361 ttggttcttc ctgtctgttt tgcgagtgac tccagacgtg tttcaatgtc atcagataaa
     3421 cgaatagcta acatggctac ctcttgatct acttttgcta tacatgtata gcaaattata
     3481 gtcgaaaact atgaaaaaga ccagaagcac ctttttctgt tagaaattaa tgtggttata
     3541 tattacccga cttgattatt tttaatgcgg tctcatatgt tcatattgag ggctttcctc
     3601 ttacaaagaa aggagattac tatgggtctt aaacctggtc cgaaacccat cgcaaaatcg
     3661 acgggaaaac cagatcaacg tcgacgcgac aataaagata ctccgggaaa tactcctgag
     3721 ttaaaaccga gcaaatccac aggtaagtga agcaatcagg gtatattatc tataccctga
     3781 cattaatgaa tgagcgaata atgtaagata caatcctaca atttacaaag cttggcgtaa
     3841 gttaaattca gtatattgtg tataatttaa cataatcgtg cttaagcgca cacccgctgt
     3901 aatggctcaa tagtaatgtc cgcctgtggc tcaattcaga tgtccgcgct atggtaagct
     3961 tcactggtcc gtttaaacta ccgggaggca tatcatgagc gcagaaagct caggagtgtt
     4021 tactttgaaa gagatcaacc ggatcaagat tatacaggac gtcattgaac gtcgcatcac
     4081 aacgcgccgt gcggccgagc acctcggtat cagcgacagg caatgccgca gacttcttgc
     4141 ccgttaccgt gaaggcggac cgcttggtat ggccagcaga cgatgtggca tgcgtggtaa
     4201 ccgccagttg ccacccgggc tcgcagatca ggctctggaa ctgatcaaga cgcgttatgc
     4261 tgatttcggt ccgactctgg cgcgtgaaaa gctcgaagaa ctccacggac tgtttcttgg
     4321 caaagaaact gtccggcgca tcatggtgcg ggctggctta tgggttcccc gtaaacaacg
     4381 tgccgcaagg atccctcaac cacggtaccg gcgtccgtgt actggtgagc tgatacaaat
     4441 agatggctgt gatcacgact ggtttgaagg ccgtggcccg gcctgcaccg cgctggtcta
     4501 tgttgatgat gcaaccagca aactgatgga actgttgttt gttaaatcgg agtccacgtt
     4561 ttcttacttc gaagccacgc ggcgctatat cgataagcat ggtaaaccgc tggcactgta
     4621 cagcgataaa gccggtgttt ttcgtgttaa caataaacac gccacaggcg gagacgggca
     4681 tactcagttt gggcgagcca tgcatgaact gaacatccag actatctgtg cagaaaccag
     4741 tcccgccaaa gggcgtgtag aacgagctca cctcacttta caggatcgtc tggtcaaaga
     4801 gctgcggtta cagggcattt gttcaatgga ggctgcaaat gacttcgctg aggcctatat
     4861 ggctgactat aaccgccgtt ttggcaaagt accgcgacat gattttgacg tacaccgtgc
     4921 tgtagaacat gatgaggacc tggggcttat tttcactgtt cgtgaaaaac gtaaagtctc
     4981 aaaatcgttg acgatacaat atgataaaat gttgtacctg attgaagaca gcgaactgag
     5041 tcgccgtgca ataggtaaat atatcgatgt gtatcactat cctgatggca gaaaagagct
     5101 gcgcctgaac ggtacgctac ttccctactc tacctacgac cgactgtcag aaatcgacca
     5161 gggcgcgatt gtcgataaca agcgtcttgg ccgaaccctg gagtttatca gtctggtgca
     5221 gagcaagcgg gataacacgc gctctcagtc aattcccgct ggagatggcc cttcccgacg
     5281 acggccaaag caggaaggga agaaatccca gcgctcactg gataatgatg acatgctcga
     5341 agcactcaaa cagcttcagt cacgttcaga ggacattttt ggtaaaagag cccgctgatg
     5401 gtactcactg gccggacagt ggttgctcac cagttgatat tgtccggcca gacccatgat
     5461 gtattattca tttcgttcag catctttatg tcctgctttt cacgggcata cttaccccat
     5521 aatgaatgaa aagaacgaac aatactgatt aactgctttt ttctttgctc cggagatagg
     5581 ctgataaaat agcagcacct gctgtcaata ttatcctcaa tgaaattaaa atcagagtct
     5641 gatgcatatt ttactgttag cataatatca ttcgacgttg ttctctcaga ctctgttaat
     5701 gctgattcta tcaggtattc aaaggcatac atgacagaca gctggttttt gtctgtctga
     5761 atataaccca ttgagtttct gagtattgag gactgtacca ggaccagagg ccataaaagc
     5821 tcaacaccat aaagcttata tgtatcatgt tgatcatact gatgttgctc aacacgacga
     5881 ttaccttccc gtgctttacg aatatcatag gcaagggata acataaatcc gtctttaact
     5941 ttaatcagtg ggctttcatc cacgatgtaa tgaatgagtt catgtaattc attcagggct
     6001 tctgaatctc cccacagtat aaaacctgca ttgttcggcg ttaactcgta tcgaagcatg
     6061 ggctcaatca tcctgttgtt tcagtcggag gaaagtctat cggctttgtt gaataaatcg
     6121 aacttttgct gagttgaagg atcagatcac gcatcttccc gacaacgcag accgttccgt
     6181 ggcaaagcaa aagttcaaaa tcaccaactg gcccacctac aataaagccc tcatcaaccg
     6241 tggctccata actttctggc tggatgatga agctattcag gcctggtatg agtcagcaac
     6301 accttcttca cgaggcagac ctcagcgcta ttctgacctt gccatcacga ctgtgctggt
     6361 cattaaacgc gtattcaggc tgaccctgcg cgctgcgcag ggctttattg attccatttt
     6421 ttctctgatg aacgttccgc tacgctgccc ggattacagc tgtgtcagca ggcgggcaaa
     6481 gtcggttaac gtcagtttca aaacgttcac ccggggtgaa atcgcgcatc tggtgattga
     6541 ttccaccggg ctgaaggtct ttggtgaagg cgagtggaaa gtcaaaaagc atggccagga
     6601 acgccgtcgt atatggcgaa agttgcatct ggcagttgac agcaacacac atgaaatcat
     6661 ctgtgcagac ctgtcgctga acaatgtgac ggactcagaa gccttcccgg gtcttatccg
     6721 gcagactcac agaaaaatca gggcagcatc ggcagacggc gcttacgaca cccggctctg
     6781 tcacgatgaa ctgcggcgta agaaaatcag cgcgcttatc ccaccccgaa aaggtgcggg
     6841 ttactggccc ggtgaatatg cagaccgtaa ccgtgcagtg gctaatcagc gaatgaccgg
     6901 gagtaatgcg cggtggaaat ggacaacaga ttacaaccgt cgctcgatag cggaaacggc
     6961 gatgtaccgg gtaaaacagc tgttcggggg ttcactgacg ctgcgtgact acgatggtca
     7021 ggttgcggag gctatggccc tggtacgagc gctgaacaaa atgacgaaag caggtatgcc
     7081 tgaaagcgtg cgtattgcct gaaaacacaa cccgctacgg gggagactta cccgaaatct
     7141 gatttattca acaaagccaa gtctatcagt aaagagaatg agagatcaaa gtggtcattt
     7201 taattgagct ggataacgga catttcaatt gagccttgac acccgcttct ggtttcatcc
     7261 tgaaaacacc ccgaacagtt caaaaccgcc tcttttcctg ccgaaaacac actgttttat
     7321 tgttactcat ttgttatcac aatagcggtt tcggtttcgc ggtaaaaatg acaccaaccg
     7381 gctatttttt ccagaattgc cacggttttt ttgttggtac tggtgcaacg ggttcaggtg
     7441 ttgtcggtag cttggattca attagaagca ttgcctgtcg tatgtcatct aaattttttg
     7501 aaagtagctc tttttcggct tttaaatgtg tattttcttg ttcaaggagt tgaagtttag
     7561 cttgtagtga ctgtgtatca ttttgtgttt ccggttgtag ttttgataca ctagtgatgc
     7621 tgtcactgat tgtagttggt aaattactta ttttgccaaa tactctaatt aattcagatg
     7681 tttcaatggt ttttttacca gtattctgat ctgtagtcgt agacagtctt ccttgtttta
     7741 tatacttgtg tagtgtgctt cttgcttttc ctgtcagtct tgccgcttca ctgattgata
     7801 ccaaagccat aatcaatcct ctaacatcat ttttatttgt tgatcttctt tgaagtgaaa
     7861 gtgtagtgca aatacactcc tgcctttttt tacggtatca actgttactg atagattgct
     7921 tttttggttt aactctttta ttgctggttt tattacccac tgattaagtg atttgaaggt
     7981 ggcgtatttt ttttcgtcca aacctagcat cgagcgaaaa tcatcaatgc tgatcaatcg
     8041 atctccaatc actttaaatt gcattaataa ctcatataaa cgaatgctat agacactttt
     8101 tagtgttgat acatttttga tcacgatttt agtaaattga ccttgaagct gagttaagta
     8161 gggcattatg gagtcagcaa aaacaatttc tatttttccc tcaccctcaa aatattctgc
     8221 ttcttcttgg atccagcgaa tacgtcgtct ttcattattg ttttgtatat atatccaacg
     8281 ttcagcgagc ttatctatag cttcttttaa atgtcgttct gcattacttt ttcccatatc
     8341 aggaaatgct agataaaaat cgccagatgt aattttgaat gttttttgag gagttttatc
     8401 ccgagggttt aactttccaa tacacatcaa tagcaagcgc tgttcctgta aagttagttt
     8461 gtaactagct tcaatcaacg cattggattt atatgctatt agttcagaca tgtcgcttcc
     8521 tactcaatgg gatagacaaa aaattagtct atccctacat taaaggcaag tcttttattt
     8581 tatctatgcc taatttgtga gtattaccgt aaatttctat gcctattacc gtaaatttct
     8641 atgcctatta ccgtaaattt ctatgcctat taccgtaaat ttctatgcct attagggttt
     8701 taattcttat aaaacaaaag gatatacata gataaaacaa gatttaaaaa caaaaaataa
     8761 tttaaaacag aaaagccctg tggataaatc aactcaatct gtcagataca tcacgttttc
     8821 ccgtattcaa aaggatgtcg caaactctgt ttatttcttt tcaaaacata ttatcaaacc
     8881 ctggtagctc ccccgcaatg cgggggcata tcgccgacag tattttttgt ctccgaccat
     8941 caagccactg aaccgctgat tcttccgtgc aattaagttc gctacgctca cggctcaatc
     9001 agaatagtgc tgtatgagct tataagcgac tttcttcgct tgtgacgatg aattccatat
     9061 tcaagatata aaaaccactc ctgattcgtt ctgtggcttt ttattgctat aaccgatatt
     9121 gcagtaagac tgtatttttc attctcaggc atattcccag aatttatgct gccgttcaga
     9181 aaacggttca tgtgactttc aaaatctgtt ttttcaaaag aacagatgca gaatcaggaa
     9241 tgaaggtggt taagaatcaa gcaatccgcc gcacagacgt caacctttca ctggattttg
     9301 atttcagatc attttaaatc aaaaggttat gatgtttttg atatccgatg aatattggta
     9361 tttacgatga tgaaagaatt tgaacagcaa gaataatgtt atttaaatta ttatttatca
     9421 aatggataaa ctaaagattg ccggttgtaa gacatgcagc taaaagattg ttctctgcaa
     9481 ggcaacaggt tcattactga gtttaaaaac caatttttca ttttcagacc agaacaggtg
     9541 ctggcatttg tcggtttaaa cgttctaagt tcaggttcga cgtctactcc atagttctga
     9601 gattgtatta atcaaaatgg caaaaacccc gaacaggata ttcatctcca ttcagggcag
     9661 tctacaaacg ttgcaatttg attttgtctc tcttcattct tgacgtcaat actaatccat
     9721 caggaatttc acccagttaa tcaccggtac aaactacgtc gccttcggct cacagtaccc
     9781 cctgtaaacc ggaaaatgac aaggttatta acccagccta aaaccctgtc tggtcataaa
     9841 ctaattatac cgtaactacc ctagtagtga cttaatacag ccaatacatt aagtcgtcac
     9901 tagggtcttg gtgacttaat aacgttatgc attaagtcac cattagggtt ttggtgactt
     9961 aatgtctttg ataattttta aaacaaacat ctctgatgaa aaagtctggt tttcatcaaa
    10021 aagacgagga taagtgtgcg gttgtttttt agccggaata tcttgtatgt atcaaccttt
    10081 cgctgaaaat acattaaacc agactgtaag ctaatgatta aaaatcaatt aattgcgatt
    10141 aattttcgtt tttttgtatc caaatttaac gattttccgc atgtgtttat cagaagtaaa
    10201 atttataaaa atattaatta tcaattattt ataatttgaa aggttgatta agggattcaa
    10261 aaatcaatct ttcattgtca aaatgaatct ggttcactaa ctgataacca cggattcaac
    10321 agttctttca cgcaatgctg acataaatcc agcttcaagt tatttccatc accgaaggta
    10381 gatccaaaac cagcctgccg atcaatcgac agaaattcat gaaattcagg atcgtctttt
    10441 tcagcgtgca aatgacatct gtcacaatat ttggcggaaa taatgataac ttcgtgcgca
    10501 gtgaaaaatt gcatatgacc tctatttgat gtcagtgtaa tctctattca atataactat
    10561 gttattgttt tttttaataa tatccatctg atatctagta attgatgcac atttccaaca
    10621 tattttgctg tatttttcac ttagtttatc tacagtattt atggttttcc tactctaact
    10681 acgagagtag cctattcagg aacagaaagc gccaggctac aataagcgtt ttatgggata
    10741 tttttgtatg taattaatat ttttttaaaa atatcatttt taatttaatt aatggattgt
    10801 gaaatggaaa atagtgaact gatgaaactt aaatcactta ttggtactca gttagaaagt
    10861 gatttagatt tagataaaat tgaaagatta attttaaatg gaaatgctat tttatttgta
    10921 ggggctggtt tttcgttaga gtctattaat gttgatggtt ataaattgcc attggcaaaa
    10981 gatttggcaa agataatttc aaaagaaaat tctagatatt ttcaaagcat aggagctgat
    11041 gaaaattata ttaagcaggt ggaaaattgt gatgacctaa tgattagctc tgatatcttt
    11101 ttaaaagaag taccacaaaa aaataattta ttaaatattt taaaaagaac tttttccgtt
    11161 aaagaagtaa ctgatgaaca agtgtctatc tgtaaaaata gttggagaaa aatatataca
    11221 acaaattatg ataatgcaat tgaattagct agcttaaaaa gtggaaagac tattacgtct
    11281 ttagatttaa ctgatgatac gggaaaatat agaagacata aagatatatg tttgcatata
    11341 aatggaaaaa tagacggcgc taatgaaaga gatctagata ataaaataaa attaacaaca
    11401 tcgtcatact tatcatctga ccaattttta aattcaccat ggtattctca atttaagaat
    11461 gatatagagc attgcagtgc tatttttttt gttggctatt caatgtatga tttagatgtg
    11521 caaaaaatac tctttaataa taaggaaatt aaaagtaaaa catttttcat aactagagaa
    11581 aattctagta aatttgataa ttataagtta tctatatttg gtaatatatt aaatatagga
    11641 attaatggtt tctcgaaaat attaaatgaa ttatcaccta gattctacgt cagtacttca
    11701 aaaagcataa tcaaagcctt gataaatatg cgttccttcg aaattcagct ttcacccatt
    11761 gggtgaaaga aaagtgctca aaaatatgtt aaattatcag cttttatgac tcgatatatg
    11821 gtaaaataat agtaagaaaa gtagtaaaaa ggggttctaa ttatgattaa taaaattgat
    11881 ttcaaagcta agaatctaac atcaaatgca ggtctttttc tgctccttga gaatgcaaaa
    11941 agcaatggga tttttgattt tattgaaaat gacctcgtat ttgataatga ctcaacaaat
    12001 aaaatcaaga tgaatcatat aaagaccatg ctctgcggtc acttcattgg cattgataag
    12061 ttagaacgtc taaagctact tcaaaatgat cccctcgtca acgagtttga tatttccgta
    12121 aaagaacctg aaacagtgtc acggtttcta ggaaacttca acttcaagac aacccaaatg
    12181 tttagagaca ttaattttaa agtctttaaa aaactgctca ctaaaagtaa attgacatcc
    12241 attacgattg atattgatag tagtgtaatt aacgtagaag gtcatcaaga aggtgcgtca
    12301 aaaggatata atcctaagaa actgggaaac cgatgctaca atatccaatt tgcattttgc
    12361 gacgaattaa aagcatatgt taccggattt gtaagaagtg gcaatactta cactgcaaac
    12421 ggtgctgcgg aaatgatcaa agaaattgtt gctaacatca aatcagacga tttagaaatt
    12481 ttatttcgaa tggatagtgg ctactttgat gaaaaaatta tcgaaacgat agaatctctt
    12541 ggatgcaaat atttaattaa agccaaaagt tattctacac tcacctcaca agcaacgaat
    12601 tcatcaattg tattcgttaa aggagaagaa ggtagagaaa ctacagaact gtatacaaaa
    12661 ttagttaaat gggaaaaaga cagaagattt gtcgtatctc gcgtactgaa accagaaaaa
    12721 gaaagagcac aattatcact tttagaaggt tccgaatacg actacttttt ctttgtaaca
    12781 aatactacct tgctttctga aaaagtagtt atatactatg aaaagcgtgg taatgctgaa
    12841 aactatatca aagaagccaa atacgacatg gcggtgggtc atctcttgct aaagtcattt
    12901 tgggcgaatg aagccgtgtt tcaaatgatg atgctttcat ataacctatt tttgttgttc
    12961 aagtttgatt ccttggactc ttcagaatac agacagcaaa taaagacctt tcgtttgaag
    13021 tatgtatttc ttgcagcaaa aataatcaaa accgcaagat atgtaatcat gaagttgtcg
    13081 gaaaactatc cgtacaaggg agtgtatgaa aaatgtctgg tataataaga atatcatcaa
    13141 taaaattgag tgttgctctg tggataactt gcagagttta ttaagtatca ttgcagcaaa
    13201 gatgaaatca atgatttatc aaaaatgatt gaaaggtggt tgtaaataat gttacaatgt
    13261 gcgagaagca gtctaaattc ttcgtgaaat agtgattttt gaagctaata aaaaacacac
    13321 gtggaattta ggtaatagtt aaagcttgtg cctgattgtt
//
