LOCUS       AP023024                4630 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus 30102 plasmid p30102_10 DNA, complete sequence.
ACCESSION   AP023024
VERSION     AP023024.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203345
            Sequence Read Archive: DRR206385, DRR206409
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 482x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30102"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30102_10"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            595..936
                     /locus_tag="BC30102_p101"
     CDS             595..936
                     /locus_tag="BC30102_p101"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33073.1"
                     /translation="MMNLLSLQQEANSLIQDLNGKDISSMSLIQKASYSNRIVKVRMSL
                     EAYRNTPGVNEFTNALDYLRVKLAEVPQQETRQQANGKRAEVIQNGLKETPYAIPQSFY
                     TVFGQTGQR"
                     /name="hypothetical protein"
     gene            948..1253
                     /locus_tag="BC30102_p102"
     CDS             948..1253
                     /locus_tag="BC30102_p102"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33074.1"
                     /translation="MIVNKQYRIPKELHEKFKATTKKTGTTMNNALCHLIAEYVYRMEQ
                     LGLPEVKESTFFHVEPVEEEIKPKPKLRKPAKKKKDGEKNEGSKKDKAEAEKDSSE"
                     /name="hypothetical protein"
     gene            1201..1446
                     /locus_tag="BC30102_p103"
     CDS             1201..1446
                     /locus_tag="BC30102_p103"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33075.1"
                     /translation="MKEVKKIKLKRKKIVRNEEHFNLEFEKARLSIEIPVSTKENFKEA
                     VEKNGKTMKDVLEAYMQRYTMKVLQEEEKQDQMIKN"
                     /name="hypothetical protein"
     gene            1936..2136
                     /locus_tag="BC30102_p104"
     CDS             1936..2136
                     /locus_tag="BC30102_p104"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33076.1"
                     /translation="MDIEAKKEAFVTFLVEKVLFQTFAERTTSLEEKAVYEKFIKLYDQ
                     ELDRLNFELTIASVNRKRANL"
                     /name="hypothetical protein"
     gene            complement(2160..2282)
                     /locus_tag="BC30102_p105"
     CDS             complement(2160..2282)
                     /locus_tag="BC30102_p105"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33077.1"
                     /translation="MKKNKKQSKKQVKKAMKVVGITKDVVNFVGNLIRIYTFFF"
                     /name="hypothetical protein"
     gene            2713..2883
                     /locus_tag="BC30102_p106"
     CDS             2713..2883
                     /locus_tag="BC30102_p106"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33078.1"
                     /translation="MPKIMKASNVRVPKTFRIQQETLDSIEELQKKEGLSQGKILDLAI
                     QLLRREKELAQ"
                     /name="hypothetical protein"
     gene            2973..3689
                     /locus_tag="BC30102_p107"
     CDS             2973..3689
                     /locus_tag="BC30102_p107"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33079.1"
                     /translation="MKKDFKTNVIDVAIREFTEAEMPNGKYYTIVPQALHFCPFLDANE
                     LRIILALSAFADGKGKDRTNQSRLKKRANISSPTTLRDKLISLEEKYFIEINSSENKNG
                     GVPLTYTLKDLHSNPYILLSEAMEEVEGLAKEYYNVSTDNIVKKLNKIVRNENVYSSMV
                     AEIPHSIEGYQVALEKVAEHIKNEFEEERAQKEEKISNVVRFKKKVAEYKKKKAQCKKT
                     TRRKKTTYTGSKSQSL"
                     /name="hypothetical protein"
ORIGIN
        1 cttaaatgat agaacaagag ctaaatataa gaagatgata ttagaagcaa gggaaagaag
       61 tggcgtggct ataaatcccg aaccaataga agtttataca aaagaaaaag acttgttagc
      121 tgagcaacta acaagcctgt tcgcacactg tgctaagagc gtagcacaag tgtaaacttt
      181 aaaacttata acaagtaaat atctaatgtt agtatagcaa aagaagagag ggaattcacc
      241 tctctttttc tttgttttaa ggggattcgt taagattata tgtatttcct ttaagattat
      301 tgagttagct tgtgttttag gattaatagt tgctatttag gttactgttt gaaaaacacc
      361 gtacgtaccc tttattgact tctacacccg aaaaccgttg cctacaccct ctcgatttta
      421 ttgagtttat ataggtacgg tgacaattta aaaataaatt caaattcttt tagtaaccca
      481 gtatacagat ttttagaaaa gggttactat gatggcaaga agaaaatatg tagattgcat
      541 agcacatcat atagcagatt tcatattttc taatcaaagg gggaagttat tacgatgatg
      601 aatttattaa gtttacaaca ggaagctaac agtttgattc aagatttaaa cggaaaggat
      661 atttcttcaa tgagtttgat ccagaaggct agttattcta atcgaatagt gaaagttaga
      721 atgagccttg aggcatatag aaatactccg ggagtaaatg aatttacaaa tgcattagac
      781 tatttgcgag ttaagttagc tgaagtacca caacaagaaa cacgacaaca ggctaatgga
      841 aaaagagcag aagtaattca aaatgggtta aaggaaacac cttatgcaat tccacaaagt
      901 ttttacactg tatttggaca aaccgggcaa cgataggagg ataaataatg atagttaaca
      961 agcaatatag aatacctaaa gagttacacg aaaaatttaa ggctactaca aaaaagacgg
     1021 ggacgactat gaataatgcc ctttgtcatt tgatagctga atatgtgtac agaatggagc
     1081 aattaggttt accagaagtg aaagaatcta ctttttttca cgttgaacca gtggaagagg
     1141 aaattaaacc gaaacctaaa ttaagaaagc cggctaagaa gaaaaaggac ggtgaaaaga
     1201 atgaaggaag taaaaaagat aaagctgaag cggaaaaaga tagttcggaa tgaggaacac
     1261 tttaacttag agtttgaaaa agcacggtta agtatagaga ttcccgtatc aactaaagag
     1321 aacttcaaag aagcggtaga aaaaaacggg aagacaatga aggatgtatt agaagcatat
     1381 atgcaacgat acacaatgaa agtattgcag gaagaagaaa aacaagatca aatgattaaa
     1441 aactaatagt aaagaagctg aattcctcga aatgataggg gataagggaa ctttttgtac
     1501 gttttagttt ttatatggta gaagtgcaaa aaacacgaca cttctttctg taggacgcta
     1561 atttacgcta aatgtattag agaaccccgc aaggaatagc ggtgaaccag gaggcattca
     1621 tgcgagggca tagtcctcgc ggtttacggt atgtcggttt ttagggaaga aatggcgagg
     1681 tttaacgagg attggcgaag aattaagggt taaattaatt ggttttcatc gagtattagc
     1741 gaagattagg gaagaccact aaggaatcaa agcgtagcgg ttcatttaca atgagaatgt
     1801 ccgtggtagc ctattatggc ttgccaatcc ttgtttgagc gttagcgagt tgaaggagtg
     1861 gggcattggc ttatgaccca cggactggag gctcgttgtc aatgattgaa ttccgtttat
     1921 tggaggaagc aagttatgga tatagaggct aagaaagaag catttgtgac ttttcttgta
     1981 gagaaagttc tatttcaaac atttgcggaa aggactactt ctttagaaga aaaggcggtg
     2041 tatgaaaagt ttattaagct atatgatcaa gaattagata gactaaattt cgaattgaca
     2101 atagcatctg taaatagaaa aagagctaac ctatagtgtt atagattagc tctttttgtt
     2161 tagaagaaga atgtataaat tcggattaga tttccaacaa aattgaccac gtcttttgtt
     2221 attccgacta ccttcattgc ttttttcact tgtttcttag attgcttttt atttttcttc
     2281 ataaattaat tcctcctata tagtagttat tcgtctttct atctattact tgtgacgttt
     2341 tttgagaaac ccttattttt tttaaaagaa atttttttct tacgttttta taggcgcagc
     2401 cattctgatt taatggcgtg atatatataa aacctgagta aaaatatcaa tttggattgt
     2461 caatcagaaa aggagttaag gatttatttt ttaggagatt actctatttg gggactgggt
     2521 gctgtaacac ccagtttact ctgtactttt tacgaccact aaactaagaa gggttttgat
     2581 ttttagttaa aactcgactg gagcggtagc ggaaagagag cattctatct tttttattag
     2641 tattagtaga caagataaat cattgatgtt aagctgtttt tatgtattac attttaagaa
     2701 aggagtgata ttatgcctaa aattatgaag gctagtaacg tacgagttcc taaaactttt
     2761 aggattcaac aagaaacatt ggattctatt gaagaattgc agaaaaaaga aggtttgagc
     2821 caagggaaga ttttagacct tgctattcaa ttattaagaa gagaaaaaga gttagcacaa
     2881 taaaatgtat atcccatcgc aaataacgta gcatactaac atagcaaaca agataacaag
     2941 gagggaaaat taaaaggaaa aggtgatcta tagtgaaaaa agattttaaa acgaatgtta
     3001 ttgatgtggc tataagggaa ttcacagaag cggaaatgcc taatggaaag tattatacga
     3061 ttgttccaca agcgttgcat ttctgtcctt tcttagacgc taatgaatta cgtattattt
     3121 tagccttgtc ggcttttgct gatggaaaag gtaaggatag aactaatcaa tcgagattga
     3181 aaaagcgggc taatattagc agtcctacga ctttaaggga taagttaatt agtttagaag
     3241 agaaatactt catagagata aattctagtg agaataagaa tggtggtgtg ccattaactt
     3301 acactctgaa ggatttacat agtaatcctt atattcttct tagcgaagcg atggaagagg
     3361 tagaggggtt agctaaggag tattacaatg tttctactga taacattgta aagaagttaa
     3421 ataaaatcgt gcgtaatgaa aatgtttatt ctagtatggt agctgaaata cctcatagta
     3481 ttgagggata tcaagtggca ctagaaaagg tagctgaaca tatcaagaat gaatttgaag
     3541 aagaacgagc gcagaaagaa gaaaaaattt cgaatgtagt gaggtttaaa aagaaggtag
     3601 ctgaatataa aaagaaaaaa gcccagtgta agaaaacaac taggcgcaag aaaacgactt
     3661 atacgggaag taagtcgcaa tctttataaa acatattgaa ctaaacaact aaggtcatta
     3721 tatattatct tttgtaaaat aaatacctaa ccaaaaattg attaggtact tagtcaaaaa
     3781 ttgattacct tttaattaaa cctaaccaat ttttgattac aactaataag atattatata
     3841 agactactaa atatatttat tctcaaaact ctctcgcaga ggttttgagt acattctata
     3901 actaaagaaa ttactaaagc atactaagat agcaaataaa acggaaaaga gtgttaggta
     3961 tggaaacaga tattagtata gaccgcctaa ctttgctagg atatgattcc ggaatgatag
     4021 agagttacct taaatatagt gagtacattt cattcgtagg attttcaaag aactatagtt
     4081 ttcgtcatat gtatcaagga ctaaacgggg agttattaga aataggcgag aaggggaaga
     4141 tacgcttaga tttcaaccct aatagagcaa atatgccaca agttagagaa atcgtttcta
     4201 gactgaaata cccctattta acgagacttg atgtagcggt agattatttt ggtatcgact
     4261 tgtctgagat tgaatggaca agtattaaaa gacggaagcg gaatttatgg ttaagccaat
     4321 cgaatgaggt agagacatta tacataggtg caccaacgag cgataaacgg tatcgtattt
     4381 ataacaaaag actagagcgg ttagagaaga aagaagaacc ggaccataga gcagttaatg
     4441 gacattggcg ggtagaggta caacaacgtt ttcaagagtc caacaacata ttagacccgg
     4501 tagattattt tgcggcggat ttattcgaca ttaagcctta tagaaaggtg gtcaatttag
     4561 atcatataga aagtgctaag gaaagaataa tgttaagagg tttattagca gagcctaagg
     4621 agctattaaa
//
