LOCUS       AP023023                5288 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus 30102 plasmid p30102_9 DNA, complete sequence.
ACCESSION   AP023023
VERSION     AP023023.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203345
            Sequence Read Archive: DRR206385, DRR206409
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 482x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30102"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30102_9"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            complement(331..585)
                     /locus_tag="BC30102_p91"
     CDS             complement(331..585)
                     /locus_tag="BC30102_p91"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33067.1"
                     /translation="MVEVVQLKPNRYGRFRKEDVLKNGYEIVEYGITATEIEKQLGVKL
                     TMKQRLKHCIGFRCEKSWRGCVPVFDILDIALKNDRNQL"
                     /name="hypothetical protein"
     gene            complement(599..862)
                     /locus_tag="BC30102_p92"
     CDS             complement(599..862)
                     /locus_tag="BC30102_p92"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33068.1"
                     /translation="MKERYSVTQKYMVAGVDEKEKVSLYTLIEVPAIKKRMVVCIGNKV
                     DGLVVGNTYEVELDISIETKKFDTKDGVKYVDVASKFVRDIR"
                     /name="hypothetical protein"
     gene            complement(1098..3638)
                     /locus_tag="BC30102_p93"
     CDS             complement(1098..3638)
                     /locus_tag="BC30102_p93"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33069.1"
                     /translation="METHECGKQSVLVQDKATKEMYGFVAQCKRMDCDSCGTRIQTYVQ
                     NKIAMYTKKFDLKHFATITMGDSDIKNLLKKFGSLVDKTRELEKEYFIKRYISRKRSTR
                     EIAEKKYYEYVHSLYDQEHYYRWLYGKDLSKKEKNINYVPISNIGYQITKMITQRPARL
                     QDQIKIILEMKQGKKLRKNQLEKYYKDNVESQRKKWVGWSYADTKKEGRREEFLNDFHV
                     LLTDIAMNMYTQLYPNEEERTIYRSKIPKENTEMAYIRTIEFHNTVDAAHIHSLFNFYI
                     PIKLLKSVMGNHVYDMAEISNLASENLEEKCEEEHDFHQYVARYVSKYITKSSLEVYEK
                     LKEEGEDRVNIISSSDNISIVLEEAFETPYKGKFQLLSVVKGVLPQHYMKFSTIQEFQY
                     FILDSIADEHPNQYIDKTISCFKDYFKEARKKGLSNRDREILKDELKANENELLQNALK
                     LELERRSKEIFVPKQLKLRKTLKQGSLTTEQFEALQGITSSDLFSFLIGYAGTGKTYTL
                     STLLDYIDFEKHDVAVVSYTAQATQRVRSMLKEQNINVDNLEINTIHKLCGAKHSMIDY
                     PIFYNDWSKPLPYSFVIIDEFSLIDIRVLTSFLASLKPTTKVLFVGDPKQLNPIHSKHP
                     YFYLKDFAGVYELTKVLRSGDRIIDVATSVRNGDIEKVPFEVMDFKNSEEKIAEYKKRG
                     YQVLSNSVALKDWINNLFQKDKKQIQSKFNYAQFDNIIFIKNDQYKRYVNGEKGQIIDY
                     NEFGVTIRTENNNEVVLKDYEFFNRVEPNHCLTVHKSQGSEYDKVLVLLDATKTLLLNR
                     NLLYTAITRAKTDVVVMINKVDDKVKTLLKQEQE"
                     /name="hypothetical protein"
     gene            complement(3814..4005)
                     /locus_tag="BC30102_p94"
     CDS             complement(3814..4005)
                     /locus_tag="BC30102_p94"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33070.1"
                     /translation="MQKAKRGRPRMNKTYRQNVVRFSYMEDTIKNIMDLVDSEERDKIN
                     ALLSNLVDHEKQKVAMKS"
                     /name="hypothetical protein"
     gene            complement(4499..4840)
                     /locus_tag="BC30102_p95"
     CDS             complement(4499..4840)
                     /locus_tag="BC30102_p95"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33071.1"
                     /translation="MYFENATHEQNYNDMMKFYSLAFGENPAFESAIYIAAYSQIHDKI
                     SKSKMDKNANPLAIALESDYLSNSAESLALLGQSLFNGEKVDVSSVVTRGKEIFEIFVQ
                     ACKIRGSLN"
                     /name="hypothetical protein"
     gene            complement(4859..5212)
                     /locus_tag="BC30102_p96"
     CDS             complement(4859..5212)
                     /locus_tag="BC30102_p96"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD33072.1"
                     /translation="MASKNKHKIIEENRDIFLKRTSGMAHDLESVSNILVNFGLAKNRR
                     IAQRWVRENRIKAEWLSGEDDRRAGKIVKESEIFKTITTEIPALKVMFNAIEELQKEND
                     ELKKQIEQSSKQK"
                     /name="hypothetical protein"
ORIGIN
        1 atcagaaatg gataatatgt cataatgctt cttcaagtcc tcaatcattt cctgcatttc
       61 ttgagataaa ccttgcaaac gaattttgat catattcaat tcctcccata attccatttt
      121 acaacaaagt catacaaagt tcaaccttaa actttatatt ttatttatat atgtatcatg
      181 caaggcatga tggttgtgag tggtacggtg taggtgtgtc tttttagctg tatagctgtg
      241 cttcccaagc acggcacagt aatccagtga caccccagca ccccacctct agcaacgcta
      301 cgcaaattta atggtaagct tacatgtgtt ttataattga tttctatcat tcttcaacgc
      361 tatatctaga atgtcaaaga caggcacaca gccacgccat gatttctcac aacggaatcc
      421 tatacaatgt ttcaatcgtt gcttcatggt caattttacg cctaattgct tctcaatttc
      481 cgttgctgtt atgccatatt ccacgatttc ataaccattc ttcaaaacat cttccttgcg
      541 gaatcgccca taacgattag gtttcaattg aaccacctcc accatgaaaa ttcataaatt
      601 agcgaatatc acgaacaaac ttactagcaa catctacata ttttactcca tcttttgtat
      661 caaacttctt tgtttcaatt gaaatatcta actcaacctc atatgtattc cctactacta
      721 atccatcaac tttattacca atacaaacta ccatacgttt cttaattgct ggtacttcaa
      781 tcaatgtata aagtgaaact ttctctttct catcaacacc tgcaaccata tacttttgtg
      841 taactgaata acgttctttc atgttttaag cacccctttt ttaatataaa tttgttaaat
      901 tataaataca tttttcttct tgtcaagttt ttctttcttt ttcctttata taacatacaa
      961 agttgaactt tatacgtttt acatttattt attttaattt tatttttatt ttatcctttt
     1021 ttttttatat ttttttactc agaaaaagac aaggtttccc ttgcctttta tagaaattaa
     1081 tataagataa aataaattta ctcttgttct tgttttaaca atgtttttac cttgtcatcc
     1141 actttgttta tcattacaac aacatcagtt tttgccctgg tgatcgctgt atacaatagg
     1201 tttctattga gtaataatgt tttggttgca tccagtaata ccaatacttt gtcatactct
     1261 gaaccttgtg atttatgaac agttaagcaa tgatttggct ctactctatt aaagaattca
     1321 taatctttta gaacaacctc attattgttt tccgttctaa ttgttacgcc aaattcattg
     1381 taatcaataa tttgcccttt ttcaccattc acataacgtt tatattggtc atttttgata
     1441 aatatgatat tatcaaattg agcataatta aattttgatt gaatttgttt tttatctttt
     1501 tgaaataaat tgtttatcca gtcttttaac gctacggagt tcgatagaac ttggtagcct
     1561 ctttttttat attcagcaat tttttcttcg ctgtttttaa aatccatcac ttcaaatgga
     1621 actttttcta tatctccatt cctcacagaa gttgctacat caatgattct gtcaccgctt
     1681 cgcaatactt ttgttaattc ataaacacca gcaaaatcct ttaaataaaa gtatgggtgt
     1741 tttgaatgta ttggattgag ttgctttgga tcacctacaa acaatacttt tgttgttggt
     1801 ttcaatgaag ccaaaaatga agttaacact cttatatcaa tcaatgagaa ctcatcaatg
     1861 attacaaatg aatatggcaa tggtttgctc cagtcattat aaaatattgg ataatcaatc
     1921 attgaatgtt ttgccccaca gagtttgtgt attgtgttta tttccaaatt atcaacatta
     1981 atattttgtt ctttcaacat gcttctaact ctttgtgttg cttgtgctgt atagcttaca
     2041 acagctacat catgtttttc aaagtctata taatccaaca atgttgatag tgtataagtt
     2101 tttcctgttc ctgcatatcc aatcaaaaat gaaaataaat cacttgaagt aatcccttgt
     2161 aaggcttcaa attgttctgt tgtcaatgaa ccctgtttca atgtttttcg caacttcaat
     2221 tgttttggta caaaaatttc tttacttctt ctttcaagtt ctaacttcaa agcattttgt
     2281 aacaattcat tttcatttgc cttcaattca tcttttaata tttctctatc tctatttgat
     2341 aatccctttt tccttgcttc tttgaagtaa tctttaaaac aagaaattgt tttatcaata
     2401 tattgatttg ggtgttcatc tgctatgcta tcaagaataa aatattgaaa ttcttgaatc
     2461 gttgaaaact tcatataatg ttgtggcaac acacctttta caacactcaa caattgaaat
     2521 ttccctttat atggagtttc gaaagcttct tccaacacaa ttgaaatatt atcactggaa
     2581 gatataatgt tcacacggtc ttcgccttct tctttcaatt tctcataaac ttctaaacta
     2641 gattttgtaa tgtactttga tacatatcta gcaacatatt gatgaaaatc atgttcttct
     2701 tcacatttct cttccaaatt ttcacttgct aaattactaa tttcagccat gtcatacaca
     2761 tgatttccca tgacactttt caataattta attgggatat agaaattaaa aagagaatga
     2821 atgtgtgctg cgtcaacagt attgtgaaat tcaatcgttc gaatgtacgc catttctgta
     2881 ttttcctttg gaattttcga acgataaatc gttctttctt cttcatttgg atacaattgt
     2941 gtgtacatat tcattgcaat atcagtaagc aaaacatgaa aatcattcaa aaattcttca
     3001 cgtcttcctt cttttttagt atctgcataa ctccatccaa cccacttttt tctttggctt
     3061 tcaacattgt ctttataata tttttctaat tggttttttc ggagtttttt gccctgcttc
     3121 atttccaaaa ttattttaat ttggtcttga agtcttgctg gacgttgcgt tatcattttt
     3181 gtgatttgat aaccaatgtt actgattgga acataattta tatttttctc ttttttactc
     3241 aaatcttttc catataacca tcgataataa tgttcttgat cataaagaga atggacatat
     3301 tcataatact ttttctctgc aatttctctt gttgagcgtt ttcgacttat ataacgtttg
     3361 ataaaatatt ctttttctaa ttctcttgtt ttatcaacca aacttccaaa tttcttcaaa
     3421 agatttttta tgtctgaatc acccattgtg attgttgcaa aatgtttcaa atcaaatttt
     3481 ttggtgtaca ttgcaatttt attttgtaca tatgtttgta ttcttgttcc acaagaatca
     3541 caatccattc tcttgcattg tgcaacaaaa ccatacatct cctttgttgc tttatcctgt
     3601 accaaaacac tctgtttccc acattcatgc gtttccatcg actctccctc ccttcttgtt
     3661 gtctaattga gaatgaaaaa aaacgtacat ggcttttttg ccccgaaacc caacgggctg
     3721 taaggctcat agctgttttc taattgagaa agtgactttt gtgcgggtat tttcgccatt
     3781 actgtaatca agttaaggga aaagtcgctc acctcaactt ttcattgcaa ctttttgttt
     3841 ctcatggtct actaaattac ttaataaagc attgatctta tcacgttctt ctgaatctac
     3901 taaatccata atgtttttta ttgtatcttc catgtacgag aaacgaacta cgttttgacg
     3961 atatgtttta ttcattcttg gtcttcctct tttggctttc tgcatctatc acacctcctt
     4021 tctatttttt tgttatacaa taaatagttt agcgcaaaat tgcaaaattc ctagtttttt
     4081 ttgcaaaaat ttttactttc atagggaatc cgcttgttcc tcgtcaaatg ataggtagta
     4141 tttttgcgaa gaccatgcaa aaatctccac ctaaacatca tttgacaagt accattttgg
     4201 aacgcttttt gtcatgcacc aagccgacat accaaaaagc tcactaattt gttttcgtct
     4261 tgtttgccta ttgtagcgtc ccaaaaacgc tcggcaaaag caaaaccttg ctgactcgtc
     4321 agcaaaccac gaaccagtac ctctacccaa aaagtctcga aaacccttct tctatgtatt
     4381 ttaacaaagt tcaatgttaa actttagact ttttaattat accaattccc tcaaagttca
     4441 accttataca ttgtatatat tatattttag taaaaaacac ttagtttatt actaagtgtt
     4501 aattcaaact accacggatt ttacaagctt gaacaaaaat ttcaaagatt tctttacctc
     4561 ttgtaacaac agaagaaaca tctacttttt ccccattgaa aagcgattgc cccaataatg
     4621 ccaatgattc tgctgaattt gataagtaat cagattccaa tgcaattgcc aatggattcg
     4681 catttttgtc cattttactt ttagaaattt tatcatgaat ttgcgaataa gctgcgatat
     4741 aaatcgcact ctcaaatgct ggattttcac caaaagctag tgaataaaat ttcatcatat
     4801 cattataatt ttgctcatga gttgcatttt caaagtacat acaaacaccc ctattttctt
     4861 atttttgttt gctcgattgt tcgatttgct tttttaactc atcattttct ttttgtaatt
     4921 cctcaatggc attgaacatt accttcaacg ctggaatctc agttgtaatt gttttgaata
     4981 tctcactctc tttaacaatt ttccctgctc ggcgatcatc ctcgccactt agccattcgg
     5041 ctttaatacg attttctcgc acccaacgtt gtgcaatcct acgattttta gctagtccaa
     5101 aattcactaa aatatttgaa actgattcta aatcatgtgc catgccactt gttcttttta
     5161 agaaaatgtc acgattctct tcaatgattt tgtgcttatt ttttgatgcc ataataacaa
     5221 cctccctatg attttaaacg catttctata taacatcgta tgtattggct attgcggttt
     5281 tggtagaa
//
