LOCUS       AP023013                3169 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus 30090 plasmid p30090_8 DNA, complete sequence.
ACCESSION   AP023013
VERSION     AP023013.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203344
            Sequence Read Archive: DRR206384, DRR206408
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 503x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30090"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30090_8"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            335..523
                     /locus_tag="BC30090_p81"
     CDS             335..523
                     /locus_tag="BC30090_p81"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD26960.1"
                     /translation="MARPKEGKLYRQRKIRFSYLEDAFRRVLDRVDDPRSRMEIQKELA
                     TLIGKEKVIEKEKEMVG"
                     /name="hypothetical protein"
     gene            562..1761
                     /gene="nicK"
                     /locus_tag="BC30090_p82"
     CDS             562..1761
                     /gene="nicK"
                     /locus_tag="BC30090_p82"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:UniProtKB:P96635"
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA relaxase NicK"
                     /protein_id="BCD26961.1"
                     /translation="MGNKQLDPRICNTGVLNTSESGLRACVDWVQATLKIAEVEHVISN
                     ILGLKNADFVEQETSKFGYKKMVRFGSIQIYYEGHSEDMGIHIHMGGQACREYEEYQVR
                     TWAQLFALIFGQGHFTRIDIAIDDFKGYFKISAIERKIKTGQLSSKFKKARHMESIEIK
                     EGKSLGRTVYFGSEHSDVQIRMYDKKLEREGKGKEVTEDFWVRTEIQTRDLHADKIARR
                     IIAGGSNVSEVNGEDDNCEMTVGETVKETLANYLRFLVKPKDGGDSNKGRWKTAPFWDK
                     FLGDVEKLKLTDVAPDRTVERSIQWLEKQVAPTLGLVFEAYDNDAVKILQVMREGVERL
                     GSKERAMLNRFRLENPDFVEKLEKKHESIAEQKQKIRKFNEEREQKKKSDSALTPYDFM
                     "
                     /name="nicK"
     gene            1906..2262
                     /locus_tag="BC30090_p83"
     CDS             1906..2262
                     /locus_tag="BC30090_p83"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD26962.1"
                     /translation="MFFIDKEHENNFNEMMDLYDLSVGQDVEYESAIYVAAHPEIFKCY
                     DLNKLDTEFGPLGCLVNGDDTHNVGALTGTTRALAQMGHALYGGNEMYVVDMCIYGEET
                     FEVFVQACRIRARL"
                     /name="hypothetical protein"
     gene            2568..2834
                     /locus_tag="BC30090_p84"
     CDS             2568..2834
                     /locus_tag="BC30090_p84"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD26963.1"
                     /translation="MKFEINGVYHFVSVKEAGKSNMYVLLDENSNKISTFGVDPEVEFE
                     KFEKVEVAITVNLKKTKTGVKNGKDNYEDVARLYLTSIKKVAE"
                     /name="hypothetical protein"
     gene            2840..3100
                     /locus_tag="BC30090_p85"
     CDS             2840..3100
                     /locus_tag="BC30090_p85"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD26964.1"
                     /translation="MQITDELRHLDVPLFLEYVCVSGDDEFDIDWWTTSELKELFGIKL
                     TDKERKTRIQAFKWGMGYRQWAAVYSKEDILNHRLVDSERA"
                     /name="hypothetical protein"
ORIGIN
        1 agcgtacttt tgacctcgaa cagtttccga cgcgggagga gtgataagat gtttggagag
       61 ccaaatggtt ggtttgaaat tgtgtttaaa atattagtct tgtgtgtatt gcctagtttc
      121 attctagtta gaatggtgta cctggactgg aaggaatcgc gaagccaaaa ggcatcgcca
      181 aaacctgacg aagatgtggc gaaacatcgg gaagattagg gagtctttag gggtcttgac
      241 gaagactctt ttaatctatc aaaaaaaatt gcaagaaata caagaatcta gtaacatagt
      301 ttgtggggca caaaaaccaa gaggagggat tcctatggca cggccaaaag aaggaaagct
      361 ttatagacaa agaaaaattc gttttagtta tttggaagat gcgtttcgta gagttttgga
      421 tagagtcgat gatccacgct caagaatgga gattcaaaaa gaattagcga cattaattgg
      481 taaagagaaa gtgatagaaa aagagaagga aatggttggt taaatgtttg ttttttttct
      541 tagttggaag aggtgaaaaa aatggggaat aaacaactcg acccccgtat ctgtaacacg
      601 ggggtgctaa atacatcgga aagtggcctg agggcttgcg tagactgggt tcaagccact
      661 ttaaaaattg cagaagttga acacgtaatt tcgaacattt tagggttgaa aaatgctgac
      721 ttcgtagagc aagaaactag taaatttggc tataaaaaaa tggttaggtt tgggagtatt
      781 caaatctatt atgaaggtca ttctgaagat atggggatac atatacatat gggtggacaa
      841 gcttgtcgtg aatacgaaga atatcaagtg agaacttggg ctcaattgtt cgcgttaatt
      901 tttgggcaag gtcattttac aagaatagat atagcgattg atgactttaa gggctatttt
      961 aagatttcgg caattgagag gaaaattaaa acggggcagt tatcatctaa gttcaaaaaa
     1021 gcgaggcata tggagtccat agaaatcaaa gaggggaagt cattagggcg gacggtttat
     1081 tttggttctg agcattcaga tgtgcaaatt cgtatgtatg ataaaaaatt ggaaagagaa
     1141 ggcaagggta aagaagtaac tgaagatttt tgggttcgaa ctgagataca aacaagagat
     1201 ttgcatgcag ataagattgc tagacggata attgcaggtg gatctaatgt gtcggaagta
     1261 aatggtgaag atgataattg tgaaatgact gttggtgaga ctgttaaaga gactctagct
     1321 aattatttgc gttttttggt aaaaccaaaa gacggtggag attcgaataa aggtagatgg
     1381 aaaacagcac cgttttggga taaatttttg ggagatgtag aaaaactaaa actgacagat
     1441 gtcgctcctg accgcactgt tgaaaggtcg attcagtggc tggagaaaca agtggctccg
     1501 acgttggggt tggtgtttga agcgtatgat aatgatgcag ttaaaattct tcaagtgatg
     1561 cgtgaagggg ttgaaaggtt aggttcaaaa gaacgtgcaa tgttgaatcg atttagatta
     1621 gaaaatccgg attttgttga aaaattagag aaaaaacatg aatctattgc ggaacaaaaa
     1681 caaaaaatta ggaagttcaa tgaagaaaga gaacaaaaaa agaagtcaga tagtgcgcta
     1741 acaccatacg acttcatgta aaaaaacgat aaaattctac ttaaattata tacagagata
     1801 cagatagatt caaggtaaag ctgaacgtta tcgttcggct ttatttattg agagggcttg
     1861 ggggtaacgt taaaaaactg ttaccctaaa aaaagaggtg attatatgtt ttttatagat
     1921 aaagaacacg aaaataattt taacgaaatg atggatttat atgatttgag tgttggacaa
     1981 gacgtggaat atgagagtgc aatttatgtt gcagcgcatc cagagatttt caaatgctat
     2041 gatttaaata aattggatac agagttcgga cctttgggat gcctggtgaa tggagatgat
     2101 actcacaatg taggagcttt aacaggtact acacgggctt tagcccagat ggggcacgca
     2161 ttgtatggtg gtaacgaaat gtatgttgtg gatatgtgta tttatggaga agagacattt
     2221 gaagtgtttg tacaagcgtg tagaattcgt gcgagattat aagtgttgga atgaaaggtg
     2281 tcaccgagag gtggcatctt tttttattgg agagatcgga gtgaaacggt tatgtcgcaa
     2341 aagcgtattt attcatagtg agtctttgct ttacactcgt gaaagtgtag ccgagaaggc
     2401 tggttttgcc ggaacggtta tggtttctaa gagtgcagag actggaatct gtgtgtaaat
     2461 atgactgtga aataaaagag tagcggataa aatgcataac aaataattga caggtttaaa
     2521 tatcaaaata caatattaaa aacttttaaa aggtgaggtg attgattatg aaatttgaaa
     2581 tcaatggtgt gtatcatttt gtgagtgtga aagaagctgg taagtcaaat atgtatgttt
     2641 tgttagatga aaattcaaat aaaatatcta cttttggagt agatccagaa gttgaatttg
     2701 agaagtttga aaaagttgaa gtagctatta ctgtgaatct gaagaagaca aaaacgggag
     2761 ttaaaaacgg taaagacaat tatgaagatg ttgctagatt atatctaacg tctattaaga
     2821 aagtagcgga gtaatagata tgcagattac tgatgagttg aggcatttag atgttccctt
     2881 gtttctcgaa tatgtgtgtg tgagtggtga tgacgagttt gatatagatt ggtggactac
     2941 gtctgaactg aaagagctct ttggaataaa gttaactgat aaagagagga aaactagaat
     3001 acaagcgttt aaatggggta tgggctatcg acagtgggca gcggtttaca gcaaggaaga
     3061 tatattaaat catcgtttag tagattcaga aagggcgtag cgcgtagctg tcctttgttc
     3121 ccgtggtcgt agactgggcg agtgcaaagg gagctcagct ttgcgataa
//
