LOCUS       AP023011                5188 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus 30090 plasmid p30090_6 DNA, complete sequence.
ACCESSION   AP023011
VERSION     AP023011.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203344
            Sequence Read Archive: DRR206384, DRR206408
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5188)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 503x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..5188
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="30090"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="p30090_6"
                     /geo_loc_name="Japan:Tottori"
                     /collection_date="2016"
     gene            complement(54..1166)
                     /locus_tag="BC30090_p61"
     CDS             complement(54..1166)
                     /locus_tag="BC30090_p61"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD26951.1"
                     /translation="MSKITQHIGMKIDQVYGKVKTDKLTFDDKNKNKKQLSDRTINTYC
                     GIVKSYNNWLEKTHGISVDRAKPRHAMEYLETKIQAYEKGNGSAFSMRQLAHALHSFRE
                     ASHKTGVFKHQCKVADKNKMLGTLTERGIFRKAGDSTSLKANHNDYEKVQKEADKSNTN
                     RHLKQEIKTVHELQRYCGMRVHEAVKLKVKDIDLANGTVYIKGKGGHERTVKIENKELL
                     DKLKDLCKGKNANSQVIIARKKDGNQLTNSEFGKRVKQEIRGAAEKAGVDRNGKKYTTH
                     SGRKAYAQAQMDKYKSYSKTQLKKEMAKRCQDKKTKNKYDKVLETIRKKITEGSENKER
                     KLTHKEMCQFLVSLDLGHYRLDVIRYYADY"
                     /name="hypothetical protein"
     gene            2407..2751
                     /locus_tag="BC30090_p62"
     CDS             2407..2751
                     /locus_tag="BC30090_p62"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD26952.1"
                     /translation="MDLATDKPKSSLFGLSAWHKPCKAPDFFSQDFKGGDFLARFMQEN
                     TTCVLSEKIALTIVIKGRIHKKVVKSQSYQGLKALFRVRGNPHTFLFFCKDILSPIFFQ
                     LLKKRLQKMN"
                     /name="hypothetical protein"
     gene            complement(2816..3499)
                     /locus_tag="BC30090_p63"
     CDS             complement(2816..3499)
                     /locus_tag="BC30090_p63"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD26953.1"
                     /translation="MAVLDLKAKLDEVERKAKSRDFANQSESEINEIKASLLEMINELT
                     GEEHYIAKSSDPLKKEPFQQMITKNLQLLVKIDYLTQAEESFLFRVQNFLEFGSNVIIC
                     KEDKNKTKKQKKKEAEELGIEVLPKAATITDIAEMVGRSRRQTSEIMNSLREKEILLNP
                     EGAGQVTEGGRTVTARTWVVNPHIILCAPRGKIDRLTQHLFRYSLKKLKDKNGKKVELP
                     VRFFV"
                     /name="hypothetical protein"
     gene            complement(3759..4244)
                     /locus_tag="BC30090_p64"
     CDS             complement(3759..4244)
                     /locus_tag="BC30090_p64"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCD26954.1"
                     /translation="MEIEIIKKVLEEQGIKGKKLEEIVAKTVLEQEKQREEELKNEEML
                     KTVEETAKILEKALDSKWNSAKVRRFIASDELKVIDPNPAKRIGFRIHTDEINRFIEWE
                     SMTKEDWKKRCVATENDLKKLEKELEIVKEENEKLKAAKTTRVKKTETNVKKDADEK"
                     /name="hypothetical protein"
ORIGIN
        1 ccagtaccaa gagcgctcag tgtctcgtct tcccgaattt gcgccaatac tcatcaataa
       61 tcagcataat accttatcac gtccaaacga taatgtccca ggtctaagct aactagaaat
      121 tgacacatct ctttatgagt aagcttgcgt tctttgttct ccgaaccttc ggttattttt
      181 ttacgaatcg tttcaagcac tttatcatat ttatttttgg tctttttatc ttggcaacgt
      241 tttgccattt ccttttttaa ttgagtctta ctatagcttt tgtatttatc catttgcgct
      301 tgtgcgtagg cttttcgacc gctatgtgtt gtgtattttt ttccgttacg atccacgcca
      361 gctttttctg ctgcacctcg tatttcttgt tttacacgct ttccaaattc agagtttgtt
      421 aattgatttc cgtctttctt tctagcaatg attacttgac tgttagcatt tttccccttg
      481 cataagtctt tcaatttatc tagtaactct ttattttcga ttttaacggt tctctcgtgc
      541 ccgcccttgc cttttatata gacagttcca ttagcaaggt ctatatcttt tacttttagt
      601 tttacggctt cgtgaacacg cattccgcaa tatcgctgta attcgtgtac ggttttgatt
      661 tcttgcttca aatgtctatt tgtatttgat ttatctgctt ctttttgcac tttttcatag
      721 tcattgtgat ttgccttcag cgaggttgaa tcgcccgctt ttcggaaaat cccacgctct
      781 gtaagtgttc cgagcatttt atttttatca gcgaccttgc attgatgctt gaacacgccc
      841 gttttatgac ttgcttccct aaatgaatgt aacgcatgcg ccagttggcg catggagaag
      901 gcggagccat ttcctttttc ataagcttga atttttgttt cgagatattc cattgcgtga
      961 cgtggtttcg ctcggtctac gctaattcca tgcgttttct cgagccaatt gttatatgat
     1021 ttcacgattc cacaatatgt gtttattgtt ctatcgctca attgcttttt gtttttgttc
     1081 ttatcatcaa aagttaattt gtctgtttta acttttccat agacttggtc tattttcatt
     1141 ccaatatgtt gcgtaatttt gctcatttcg tcacccccta aacccaaacc caagagcaaa
     1201 gacccaaccc cacgaacctt acaatatcaa ggcacgagaa aaaggaggtc tcatttgggt
     1261 gggttcgatc cattttttta aaaagcaaaa aaaatagagc gaaccaccca aatcaaagag
     1321 catacaaagc aatcgcggat tctcccttcg ggcgaattat gctcacacaa accaaaatca
     1381 aaatcaaaag accaagagca aaagacaaag gtcaaaagac aaaggtcaaa agattaaaag
     1441 ataaaggctc atgatagcta cgccatcatg ttgcgattgg cgtctataaa tagactccaa
     1501 ggttctctcc tatacgtatt gcaccaatca cgtatagtca aacgagtggt cttgcggtct
     1561 ggaggttttg agtaacccag cgttactgtt ctttgcgatt gtattttgta gctaaaaacc
     1621 tttttgtttc agtaaatcta tttagttgta aatctctttt ccatcgtatt ttcgattatc
     1681 atctccttaa atttttattt tttataatat ttttttccat tcttgcaagc aagaattcta
     1741 ttttctttta aagctattga taaaactact tgggaaagct ttatttcgct ccttcttaga
     1801 ttattttttt ccacatctta aaaatatttt gctttgctat tgtaatgatc ccctttcatt
     1861 tttatgacgt gaatttttta tttttgtaaa acacacgatt acttgaatca cgtcccaaga
     1921 ttcatgagta aaatttcgta tttcatattt aattgaccac acgtgttcaa tcaaataagt
     1981 aaatggtttt aaaattacta tgaatatata caaattttac cataaatcaa ctgattttga
     2041 taaaaaaaac cttatttttc atgcttttga aaaggtcttg cgaccttaat tcttttatgt
     2101 tttttcctaa gcaaaaaagt gtttttggtt tttatgattc tgaacgtaaa aaatccgtaa
     2161 ttcttaaaat caagaaccac ggattttgaa ccgtatcaaa tttaatacgg ttcaaaaact
     2221 acgaaaaagc ttgttatatc aatctatttt gcggttatca aaattggtaa ctacgaattt
     2281 atggttttac cttgcttttt gggttgaggc tggcgcgtag tgtggtgtca agacaccacg
     2341 aacttttctt ttcaaacaaa aatattgata gaacacaaat gtacgctcgg cgttgaggtg
     2401 cttacaatgg atttggcaac cgataagccg aaaagctcgc tttttggttt atcggcttgg
     2461 cacaagccat gtaaagcacc ggattttttt agtcaagact ttaaaggtgg agattttctt
     2521 gcacgtttta tgcaagaaaa tactacctgt gtcttgagtg aaaaaatagc cctaacaata
     2581 gtgataaaag gaagaataca taaaaaagta gtaaaaagtc agtcatacca agggttgaag
     2641 gcacttttta gggtgagagg aaatccgcac acttttttgt ttttttgtaa ggatatcctc
     2701 tcacctattt ttttccaatt actaaaaaaa cgactacaaa aaatgaatta atatacaaaa
     2761 aaatgaatta atatactgtt tgaattataa aaaaatgcta ctgaaaagta gcattttaaa
     2821 caaaaaatct aacaggcaat tcaacttttt tgccattttt gtcttttaat ttcttcaatg
     2881 aatatctgaa cagatgttga gtcaatctat caattttccc tcgtggtgcg caaagaataa
     2941 tatgaggatt cacaacccaa gttcttgcgg taacagttct accaccttct gttacttgcc
     3001 cagccccttc gggatttaat aaaatctctt tttctcgtaa agaattcatg atttctgatg
     3061 tttgtcttct tgaacgtcca accatttcag caatatcagt aatagttgcc gcttttggta
     3121 aaacttcaat acccaattct tcagcttctt ttttcttttg cttttttgtt ttgtttttgt
     3181 cttctttgca aataattaca ttacttccga actcaagaaa attttgaaca cgaaacaaaa
     3241 acgattcttc agcttgtgtt aaataatcaa tctttacaag aagttgtaaa ttcttcgtaa
     3301 tcatttgttg gaacggctcc tttttgagtg gatcactgga ttttgcaatg taatgttctt
     3361 cgcctgttaa ttcattaatc atttctaaaa gtgaagcctt aatttcattg atttcacttt
     3421 cactttgatt cgcaaaatct cttgattttg ctttacgttc cacttcatca agctttgctt
     3481 ttaaatctaa aacagccatg tttcttgctc ctctcctaaa tgcgacaagc caaaaatagg
     3541 agatttcacc acgtttttat ttatccttaa gagcttaatt gcccttgctt tttctctata
     3601 aagttattaa aattaaagta gaaaatcatc gtttttgttg gtgaattgaa tcacctgttg
     3661 tggtgaaaaa tcacctagtc gagtgttgac gcactcgact ttttttatgc ttgtctactt
     3721 acaattctag cttaattttt cgaattttgt aagatgcttt acttttcgtc agcatctttt
     3781 tttacgttcg tttcagtttt ctttactcta gttgtttttg ctgcttttaa tttttcgttt
     3841 tcttctttta caatctctaa ttctttttct agctttttaa ggtcattttc cgttgctaca
     3901 caacgttttt tccaatcttc cttagtcata ctttcccatt cgataaaacg gttgatttcg
     3961 tccgtatgaa tgcggaatcc gattcttttt gctgggtttg gatcaattac ttttaattca
     4021 tctgaagcga taaatcgacg cactttcgca ctattccact tgctatcaag cgctttttca
     4081 agaatttttg ctgtttcttc aaccgttttt agcatttctt cgttttttaa ttcttcttca
     4141 cgttgttttt cttgctctaa cactgttttt gcaacgattt cttcaagttt tttgcctttg
     4201 attccttgtt cttctaaaac ttttttaatg atttcgattt ccataaaatt tattcccctt
     4261 tcgtatatcc aactgttttt ataattaata ttaattcgtg atgtattttt aatatcaatg
     4321 ttttatttca ttttgttttg ttattaattt tattatacat cttttatttt taaagtcaat
     4381 ggtaattaat ataaattttt atagataatt attttcgatt ttcgtaaatt cggttgacgt
     4441 taacgaattg aaatttatta aaaatagcga taaatcaacg atttatcgct atttttgact
     4501 ttcgattttc gtatcaatta ccgaaaacaa acctcatttt catctcaaaa atatttgata
     4561 taaaatttaa tattgttttt atttacgtta attttgtttc atatcaattt tgtttcatat
     4621 taaaaaaata tatcttttta ttctcaaaaa gttttatatt tttatattat tttaaaatat
     4681 ttattgatat taattttatt aaaaaataat caattttaac acaaaaataa attgtcataa
     4741 aataattata ttaacaaact attttaatat aaaatatatt tgttataaat attattttac
     4801 aacaatttta tttaatgttg attttgattg atattaaatt attttgactt tgttaacggt
     4861 tatttcacaa aaaattgatg aaaaaagagc attttttttt tgaaaatcga aaaaatgccg
     4921 tcaaatcaac gaaaattaca actctgacgt atcttcttag ttcatctacc catttgacga
     4981 ggattggcgg agacatggcg agacgtggcg aagatattca ctttgctttt gtatcttttt
     5041 agcgcatcgt ttcgaggttt ggcgataccc tcacgaccgc gcaaggaact ggcaatgcct
     5101 gcacaggcta acacggagat tctcgttata aaggcggtaa agcatgcctt gacaacgagc
     5161 ctccactccg tgtttcttag ccagtgcg
//
