LOCUS       AP022901                3287 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus MRY14-0075 plasmid pMRY14-0075_7 DNA, complete
            sequence.
ACCESSION   AP022901
VERSION     AP022901.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203326
            Sequence Read Archive: DRR206366, DRR206390
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 316x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0075"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0075_7"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            complement(3..572)
                     /locus_tag="BCM0075_p71"
     CDS             complement(3..572)
                     /locus_tag="BCM0075_p71"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010942355.1"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="BCC21402.1"
                     /translation="MAGSKVAYIRVSTVDQNLEQQKELLGKYGIDRWYEEKASAKDTNR
                     PKFQEMMDWVREGDTVYIRDFSRLARSTQDLLNITTTLKEKGVNLVSDKENIDSSTPHG
                     ELMLTMIAAINQFERANMLERQREGIAIAKERGAYKGRKHVEKPSNWDEVYEKWKSRGM
                     TAKQAMEELGLKRTTFYKLVKEVEEQ"
                     /name="integrase"
     gene            895..1152
                     /locus_tag="BCM0075_p72"
     CDS             895..1152
                     /locus_tag="BCM0075_p72"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21403.1"
                     /translation="MSNVEGLRVLVDIDKLPIHQQIRVKRVAEGLTQTQIVELLGLPDV
                     PFLSRIETGEKPIPKRFMERILDYLYEEEYEDGQLRTMAD"
                     /name="hypothetical protein"
     gene            1118..1336
                     /locus_tag="BCM0075_p73"
     CDS             1118..1336
                     /locus_tag="BCM0075_p73"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21404.1"
                     /translation="MKMDNYEQWLINENLNPFTSEGQREANHNLLRLYKAVTREKPLHE
                     SYKGLDVITERLEDDSYRCTPNKLLQS"
                     /name="hypothetical protein"
     gene            2085..3191
                     /locus_tag="BCM0075_p74"
     CDS             2085..3191
                     /locus_tag="BCM0075_p74"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_011861953.1"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="BCC21405.1"
                     /translation="MGEKMKKEYLHPYSVPPYTNRGVERTWESGLRACVDWVEATFKIV
                     DSNQLILDILQMKIDDFFLAGGKNGYRQSLQCGSISIYFDGREDMGIHLEMKGRGCREY
                     ERYGKRTWKDLFSSIRENQGYFTRLDPAIDDFDGYFTVKGLVRKVKNRELVSPFKMANN
                     MESIQISTGEVGGVTLYFGSPRSDIRIRMYDKLSERKDGNYNVSEDIKVWNRTELQLRD
                     EKATAVAEIIATKEDGEETIGQTVCGILKHYLRFTVKGKDTNRRRWKTAPFWDKFLGKV
                     EKLPLTTVIEDKTVFDKQEWVRKQIAPTLGLIYQAYDGDMEQIEQIVKEGCHRLKGKDY
                     DMIRRHKEDIKKDSQRLPNGESLTDNAI"
                     /name="Cro/Cl family transcriptional regulator"
ORIGIN
        1 ttttattgtt cttctacttc ttttactagc ttatagaacg ttgtacgctt cagccctagt
       61 tcttccatgg cttgcttggc ggtcattcct ctacttttcc atttctcata tacttcgtcc
      121 caattagacg gcttttctac atgcttacgt cctttatatg ctcctctttc ttttgctatg
      181 gcaatccctt ctcgttggcg ttccaacata tttgctcttt caaactgatt aattgcagcg
      241 atcattgtca gcattaattc tccatgtggc gtactactat caatattttc tttatcgcta
      301 acaagattaa cgcctttctc tttcaatgtc gtggtgatat tgagtaggtc ttgtgtgctt
      361 cttgctaggc gtgaaaaatc tcttatgtat actgtatctc cttcccgaac ccaatccatc
      421 atttcttgaa acttaggtct atttgtatcc tttgcagatg ctttttcctc ataccatcta
      481 tcaattccgt actttcctaa caattctttt tgttgctcca agttttggtc tactgtacta
      541 acacgtatgt aagctacttt tgaaccagcc ataatatcac tctctttccg tcttatttat
      601 agtttaatta tatcaattta tccgtttaga ctcaaggata tccacgtaca cttattcatt
      661 aattcgaaaa acaactctaa acggactgta tttatgcata tttaaatgtc tgcttagact
      721 acgtcctaaa tgaacaaatt aattgtaaaa agtattgaac taatgccaac taagggttac
      781 aaataaattg tacattagtc aatgtactga ccgctctcct tggttattgt catagagccc
      841 acttcctagg ctgagtgggc tacataacta cactccgaaa ggatttgata atagatgagt
      901 aacgtagaag gactacgtgt attagtggat atagataaat taccgattca ccaacagata
      961 agggtaaagc gtgtagcgga ggggctgaca caaactcaaa ttgtagaatt actaggctta
     1021 cctgatgtgc cgtttctttc taggattgag acaggggaaa agcctatccc taaacggttc
     1081 atggaacgca ttttagacta tctttacgag gaggaatatg aagatggaca attacgaaca
     1141 atggctgatt aatgagaact taaatccatt tacaagtgaa gggcaaagag aagccaatca
     1201 caatctttta agactgtata aagcggtgac aagggaaaag ccactacatg agtcctacaa
     1261 gggcttagat gtcattacag agcgtctaga agatgattct tacagatgta cgccaaataa
     1321 actactccaa tcatagtgaa acgattatgt tcgaaggcag ttatgagaaa gagtggaata
     1381 ggcgttagcc ttggcggaat gcttgccgtg ttctttcaaa cggctggcag tacggcaaat
     1441 gctcgtctta ttcagagtct ctgctcatta tctggagtcg aaggataaat agtggaacga
     1501 acaacgatag tacgccaaag gtctccttat ttcagagcgt ggatcataac ggcagcagcc
     1561 atcaatatta tgggacgtgt atacagccga cggcttaccc tgggtaccct cgtttcttcg
     1621 tttggggcaa tgagtaacgt ataccttgcg acatatggcg aggatatttt tttgtcctag
     1681 tagcaaattt tttgtaaggg tgtaagtgat gacatttaaa cggttcagat tggtttgtaa
     1741 gaagagttct gacaaacgtt gatataacaa cattccttgt tattaaagta gcaagtgggt
     1801 cgcagaagga ggtcaaatgg caaactctga taagatagag gtaatggcaa atgtcttccc
     1861 tatcattcag caaagttctt tatgattccc taaaacacca ctagaagctt catgttccga
     1921 cttttagcgg agacaggaca aatccttggg cggtgccatt gccgtgctct acggcttcgc
     1981 tacgatgtat agaaaataac atttgtcttt tgtgatgtga ttgagttaat gtgaaataca
     2041 agggagtttt tctccttttg ttatttgata atttgatact ggaaatgggt gagaaaatga
     2101 agaaagaata tctgcatccg tacagcgtac ccccgtacac taacaggggg gtggagcgta
     2161 catgggaaag tgggctgaga gcttgtgtag actgggttga agcgactttc aaaattgtag
     2221 actcgaatca attaattttg gacattttac agatgaaaat tgatgacttt ttcttggctg
     2281 gtggaaagaa tggttacaga caatctttac aatgtggaag tatctcaatt tacttcgatg
     2341 ggcgtgaaga tatgggtatc catcttgaaa tgaaaggtcg aggatgtaga gaatacgagc
     2401 gctacggaaa acgaacatgg aaagacttgt ttagttccat cagagagaat caaggttact
     2461 ttactagatt agaccctgcg attgatgact ttgacggcta tttcacggta aagggacttg
     2521 tgaggaaggt aaaaaataga gagttggttt ctccgtttaa aatggctaac aatatggagt
     2581 cgatacaaat atcaactgga gaagtcggtg gagtgacact gtattttggt tccccacgat
     2641 ctgatattag aatccgaatg tatgataagt tatctgaacg taaagatggc aactacaatg
     2701 tatctgaaga catcaaggtg tggaatagaa cagagttgca attgagagat gagaaagcga
     2761 ctgcggtggc tgaaataatc gctacaaaag aagatggaga ggaaactatt ggacaaactg
     2821 tatgcggtat attgaagcat tacctccgtt ttacggtaaa agggaaagat accaatagac
     2881 gtagatggaa aactgctccg ttttgggaca agttcctcgg caaagtagaa aaactacctt
     2941 taacaacggt tattgaagat aagactgtgt ttgataaaca agaatgggta aggaaacaga
     3001 ttgctcctac attagggctc atatatcagg cttatgatgg cgatatggag caaatagaac
     3061 agattgtaaa agagggttgt catcgcttga aaggaaaaga ctatgatatg atacgcaggc
     3121 ataaagaaga cataaaaaaa gactctcaac gtctcccaaa cggtgaaagt ctcactgata
     3181 atgctatctg attagctact gatagtatat tataaccttt atgtataata caataacctc
     3241 acataacttg tcataaactt acatggctct gtcgcttaga cagggct
//
