LOCUS       AP022900                3462 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus MRY14-0075 plasmid pMRY14-0075_6 DNA, complete
            sequence.
ACCESSION   AP022900
VERSION     AP022900.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203326
            Sequence Read Archive: DRR206366, DRR206390
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 316x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0075"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0075_6"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            92..661
                     /locus_tag="BCM0075_p61"
     CDS             92..661
                     /locus_tag="BCM0075_p61"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_010942355.1"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="BCC21398.1"
                     /translation="MAGSKVAYIRVSTVDQNLEQQKELLGKYGIDRWYEEKASAKDTNR
                     PKFQEMMDWVREGDTVYIRDFSRLARSTQDLLNITTTLKEKGVNLVSDKENIDSSTPHG
                     ELMLTMIAAINQFERANMLERQREGIAIAKERGAYKGRKHVEKPSNWDEVYEKWKSRGM
                     TAKQAMEELGLKRTTFYKLVKEVEEQ"
                     /name="integrase"
     gene            complement(790..1842)
                     /locus_tag="BCM0075_p62"
     CDS             complement(790..1842)
                     /locus_tag="BCM0075_p62"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21399.1"
                     /translation="MDYKEQHGYKKKLQRYQEMMIEQETAYEKTKESYPNYVEPYDEKL
                     VISGFHIIHSQYEKPIWRGFKRNDEAVEQGAKNRKISMTIRKELDNELQNYVDRFPSAT
                     KHDKQAMKELKRIKDAYKDVLQIREDNEKRKIRTLKNKIQANFDEWTHFLTLTFKENLI
                     DITRAKDKLKEWVKKMKVLYPDFLYVYVMEFQERVAIHFHVLCRMDKGKVVPKKKFMQT
                     RKTWDKLGNIDIKGIRYKYLPKKHVNEAKTELQELSASERMVTIWSVGNYLTSYLEKGA
                     DDVLLFGSKMYGASSGLKEEIVLRDKKRIAAILLELGVERLKEKTYEIKQTETDNTITM
                     KCYNQLILKN"
                     /name="hypothetical protein"
     gene            complement(2790..3008)
                     /locus_tag="BCM0075_p63"
     CDS             complement(2790..3008)
                     /locus_tag="BCM0075_p63"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21400.1"
                     /translation="MKMDNYEQWLINENLNPFTSEGQREANHNLLRLYKAVTREKPLHE
                     SYKGLDVITERLEDDSYRCTPNKLLQS"
                     /name="hypothetical protein"
     gene            complement(2974..3231)
                     /locus_tag="BCM0075_p64"
     CDS             complement(2974..3231)
                     /locus_tag="BCM0075_p64"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21401.1"
                     /translation="MSNVEGLRVLVDIDKLPIHQQIRVKRVAEGLTQTQIVELLGLPDV
                     PFLSRIETGEKPIPKRFMERILDYLYEEEYEDGQLRTMAD"
                     /name="hypothetical protein"
ORIGIN
        1 attaatgaat aagtgtacgt ggatatcctt gagtctaaac ggataaattg atataattaa
       61 actataaata agacggaaag agagtgatat tatggctggt tcaaaagtag cttacatacg
      121 tgttagtaca gtagaccaaa acttggagca acaaaaagaa ttgttaggaa agtacggaat
      181 tgatagatgg tatgaggaaa aagcatctgc aaaggataca aatagaccta agtttcaaga
      241 aatgatggat tgggttcggg aaggagatac agtatacata agagattttt cacgcctagc
      301 aagaagcaca caagacctac tcaatatcac cacgacattg aaagagaaag gcgttaatct
      361 tgttagcgat aaagaaaata ttgatagtag tacgccacat ggagaattaa tgctgacaat
      421 gatcgctgca attaatcagt ttgaaagagc aaatatgttg gaacgccaac gagaagggat
      481 tgccatagca aaagaaagag gagcatataa aggacgtaag catgtagaaa agccgtctaa
      541 ttgggacgaa gtatatgaga aatggaaaag tagaggaatg accgccaagc aagccatgga
      601 agaactaggg ctgaagcgta caacgttcta taagctagta aaagaagtag aagaacaata
      661 aaaagccctg tctaagcgac agagccatgt aagtttatga caagttatgt gatgttattg
      721 tattatacat aaaggttata atatactatc agtatcatat tagtatttca ctgtttgccg
      781 acagtggaat tagttcttga gtattaactg attgtagcac ttcattgtaa ttgtgttatc
      841 agtctcagtt tgttttatct cataagtttt ttccttgagt cgctctactc ctaactccaa
      901 taggattgca gcgattcttt ttttatctct taaaacaatt tcttctttca aaccacttga
      961 agccccatac attttagaac caaacaagag tacatcatcc gcacccttct ccaaatagct
     1021 ggtgagataa ttaccaacac tccatatagt aaccatacgt tcactcgcag aaagttcctg
     1081 taactccgtt tttgcttcat tcacgtgctt tttaggaaga tatttgtatc taatcccttt
     1141 tatatcaata ttccctagct tgtcccatgt ctttctagtc tgcataaact tctttttcgg
     1201 taccactttc cctttatcca ttctacataa cacatgaaaa tgaatagcca ctcgctcttg
     1261 aaactccata acgtacacat acaagaaatc aggatataac actttcattt tctttaccca
     1321 ttctttcaat ttatctttag ctcgtgttat atcaattaaa ttttctttga atgttaatgt
     1381 gagaaaatga gtccattcat caaaattagc ttgtatctta tttttcaatg tacgtatctt
     1441 tctcttttcg ttatcttctc gaatctgcaa gacatctttg taagcatctt ttattctttt
     1501 cagttctttc attgcttgtt tatcgtgttt agtagctgaa gggaaacgat ccacgtaatt
     1561 ttgaagctca ttatccaact ctttacgtat agtcatacta attttacggt tcttagctcc
     1621 ttgttctacc gcttcatcat tccttttaaa tccacgccat ataggttttt catattgact
     1681 gtgtatgatg tgaaagccag atataacaag tttctcatca taaggttcaa cgtaatttgg
     1741 ataagattct tttgttttct cgtaagcagt ttcttgttca atcatcattt cttgataccg
     1801 ttgaagcttt ttcttgtatc catgttgttc tttgtaatcc acctcagtgc aaacctcctt
     1861 ttcgtttaaa attagctcaa attagctcaa aattagtgca aaagattttt ctgttaaggt
     1921 ggcttaaacc cttatggttc taaggttcaa gagctctaca gggagaatag ttttttcccc
     1981 tgatttgagt tgtatattaa atctattacc cccgccccca cagtctcact tttaaccaat
     2041 gcatcgtgaa accgcacttc agatatggcg gttaaatccc acccttgtat ttcacattaa
     2101 ctcaatatca ttacaaaaga caaatgttat tttctataca tcgtagcgaa gccgtagagc
     2161 acggcaatgg caccgcccaa ggatttgtcc tgtctccgct aaaagtcgga acatgaagct
     2221 tctagtggtg ttttagggaa tcataaagaa ctttgctgaa tgatagggaa gacatttgcc
     2281 attacctcta tcttatcaga gtttgcgatt tgaacccctt ctacgaccca cctgctactt
     2341 taatagctag gaatgttgtt atatcaacgt ttatcagaac tcttcttaca aaccaatctg
     2401 aaccgtttaa atgtcatcac ttacaccctt acaaaaaatt tgcttatagg ataaaaaaat
     2461 accctcgcca catagtgcaa ggtatacgtt actcattgcc ccaaacgaag aaacgagggt
     2521 acccagggta agccgtcggc tgtatacacg tcccataata ttgatggctg ctgccgttat
     2581 gatccacgct ctaaaataaa gagacctttg gcgtactatc gttgttcgtt ccactattta
     2641 tccttcgact ccagataatg agcagagact ctgaataaga cgagcatttg ccgtactgcc
     2701 agccgtttga aagaacacgg caagcattcc gccaaggcta acgcctattc cactctttct
     2761 cataactgcc ttcgaacata atcgtttcac tatgattgga gtagtttatt tggcgtacat
     2821 ctgtaagaat catcttctag acgctctgta atgacatcta agcccttgta ggactcatgt
     2881 agtggctttt cccttgtcac cgctttatac agtcttaaaa gattgtgatt ggcttctctt
     2941 tgcccttcac ttgtaaatgg atttaagttc tcattaatca gccattgttc gtaattgtcc
     3001 atcttcatat tcctcctcgt aaagatagtc taaaatgcgt tccatgaacc gtttagggat
     3061 aggcttttcc cctgtctcaa tcctagaaag aaacggcaca tcaggtaagc ctagtaattc
     3121 tacaatttga gtttgtgtca gcccctccgc tacacgcttt acccttatct gttggtgaat
     3181 cggtaattta tctatatcca ctaatacacg tagtccttct acgttactca tctattatca
     3241 aatcctttcg gagtgtagtt atgtagccca ctcagcctag gaagtgggct ctatgacaat
     3301 aaccaaggag agcggtcagt acattgacta atgtacaatt tatttgtaac ccttagttgg
     3361 cattagttca atacttttta caattaattt gttcatttag gacgtagtct aagcagacat
     3421 ttaaatatgc ataaatacag tccgtttaga gttgtttttc ga
//
