LOCUS       AP022899                3869 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus MRY14-0075 plasmid pMRY14-0075_5 DNA, complete
            sequence.
ACCESSION   AP022899
VERSION     AP022899.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203326
            Sequence Read Archive: DRR206366, DRR206390
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 316x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..3869
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0075"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0075_5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            55..645
                     /locus_tag="BCM0075_p51"
     CDS             55..645
                     /locus_tag="BCM0075_p51"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:NP_832736.1"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="BCC21395.1"
                     /translation="MNILWDFDGTLFNTYPAYTKILSLVLGEEIDKQEIYKKLKISYSH
                     ALDYYNVSSDQKEKIKSLKKELSPKDMKPFDGVEEILKFADKNVIMTHKHRDGVLAILK
                     YYGWDKYFVDMVTIDNGFPRKPNPSAYTYLHQKHKIDLAIGDRELDLLPAKELGIITCM
                     FQGQSDVADYYLNDYSEFFNKGIPLSKQLYLQQ"
                     /name="phosphoglycolate phosphatase"
     gene            1275..1817
                     /locus_tag="BCM0075_p52"
     CDS             1275..1817
                     /locus_tag="BCM0075_p52"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21396.1"
                     /translation="MDKNSQEKITIELNADEFKKLQKLLELDEKNEQDQQKKESEKKNL
                     NFIQLYRDNMPELRWLMANHNFASSLLFFIIEHMDNKNALACSYSTFEDYFGKSKMTIY
                     RGIKVLEENGFLSVLKMGTSNVYLVNEDLAWTDSNDKKKFTKYDGKILVSKKENKDYIY
                     RKQFDRFKALRKRENLK"
                     /name="hypothetical protein"
     gene            complement(1854..3134)
                     /locus_tag="BCM0075_p53"
     CDS             complement(1854..3134)
                     /locus_tag="BCM0075_p53"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000119405.1"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="BCC21397.1"
                     /translation="MSMSVSLKKATNKTNIRHNNRTMSEKEKEQNSHIDYSRSDENKYL
                     VQKDLKKLYREEFGEVLENYNAKQKRNDRKIDDYYKHIQSSKKTSLQQEMIIQVGDKDD
                     FSSKQDFEKANEILEEWLLGFEKRNPNLKIYNAVIHNDEASPHMHLNFVPVASGYKRGL
                     EKQVSFDRAITQQDPKLDKTRPFDDWREKEVKILEEMLKERGIERKLVGSNNYKDVNEY
                     KEKKDLEKEILSLENQLFEKKNELLEMNERLPGEIKIKAEREKKTEVVNKGFLKKEKVE
                     KPTGNWIVETKELKRVQGIINAGYSVKKDYDRLQRMDLVKENKELCDKVDSLAEGYVKA
                     INENTDLENENRELRKEISSLKAHIRDLKENVKVLYHNTKKLLGRQFEGFRGLIKNELD
                     MKGIDNQFEREHKKEMKTKQRGYDMER"
                     /name="Plasmid recombination enzyme type 2"
     Typing          1276..1817
                     /name="rep_cluster_1931"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1858..3068)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aagagtatgg ctaccacatt cttgtgataa aatggaatag aaagggtgat ttaattgaat
       61 attttatggg actttgacgg aacacttttt aatacatatc ctgcctatac caaaatactc
      121 tctctagtac ttggagaaga gatagataaa caggagattt ataaaaaatt aaagatttca
      181 tactcacatg cactagacta ctataatgtt tctagtgacc agaaagagaa aattaaaagc
      241 ttaaaaaagg aattgtctcc aaaagacatg aaaccatttg atggcgtgga agaaattttg
      301 aaatttgcag ataagaatgt aatcatgaca cataaacaca gagatggagt tttagcaatt
      361 ttaaaatatt atggatggga caaatacttt gtagatatgg ttacgattga taatggtttt
      421 cctcgcaaac ccaacccatc agcatatact tacttgcatc aaaaacacaa aattgatcta
      481 gctattggag atagagaatt agatttgcta cctgcaaaag aattaggaat cataacatgc
      541 atgtttcaag gtcaaagtga tgtagcagat tattatttaa acgattactc agaatttttc
      601 aataagggta ttccattatc aaaacagttg tatcttcagc aataaatcgc tgaagatata
      661 tgatagattt attgacttcc gctttcgaat tttatgtaaa taagctgtcc atatggacag
      721 cttattttat tttttatttt aaacgatcgt cttgtttttg ggtggagtgg tgaaggctgg
      781 ggtgaagcga agcgaggggc tagcccctaa gaacttaacg taactgaata tagagtaagc
      841 ttattgctta gatcgataaa actgggtggt tcggctggta caattgctgg tccaagtcgg
      901 gcaacttaac ctcgtttttg gtctgttcgg cttggcgaaa gctaaaaacc agccgaattt
      961 cggaatgcaa tggaaaaaag gtggagattt tttgagtggg tttctcaaaa aatactacct
     1021 gcccattgcg tggagaaaaa ccctattagg agatatagaa agggaacgta agagaagcct
     1081 tgatatataa gggtttcaga gtttaatagg tatcatagtg tgatacctat aggtaacata
     1141 gcttgtgata cctatttgta gaagaatata acaaatttgt ctttacatat atcaggtcat
     1201 gttataggat ataacaaaga ttatacatat aggtatcata gtgtgatacc ttcttgcaaa
     1261 aaaggggtgg aaaaatggac aagaactccc aagaaaaaat cactatagaa ttaaacgcgg
     1321 atgaatttaa aaaacttcaa aaattattag aactagatga aaaaaatgaa caagatcaac
     1381 agaaaaaaga aagtgaaaaa aagaatctta atttcattca actttatcga gataacatgc
     1441 cagaactaag atggttaatg gcaaaccaca atttcgctag ttctctttta tttttcataa
     1501 tcgaacacat ggataacaag aatgcattag catgctcata ttcaactttt gaagattact
     1561 ttggaaaatc aaaaatgact atttatagag gaatcaaagt acttgaagag aacggatttt
     1621 tgagtgtact aaaaatgggt acatcaaatg tttatttagt aaatgaagat ttagcgtgga
     1681 ctgattcaaa cgacaagaaa aaatttacaa aatacgacgg gaaaatctta gtcagcaaaa
     1741 aagaaaataa agactatata tatcgtaaac aatttgatag atttaaagct ttgcgaaaac
     1801 gggaaaacct aaaataaagc gggtagtaaa gtctactcgc tttatttatg ttttcatcgc
     1861 tccatgtcat accctcgttg tttagtcttc atttcttttt tatgttcacg ctcaaattga
     1921 ttatctatcc ctttcatatc caactcgttc ttaataagac ctctaaagcc ctcaaattgc
     1981 cttcctaaga gtttttttgt gttatggtat aaaaccttta cattttcctt taaatctctt
     2041 atatgggctt ttaacgagct tatttcctta cgcaattctc tattctcatt ttccaaatct
     2101 gtattttcgt taatagcttt aacataacct tcagctaaac tatcaacttt gtcacataat
     2161 tctttgtttt ctttaactaa atccatacgc tgcaaacgat cataatcttt tttcactgaa
     2221 tatccagcgt taattattcc ttgcactctt tttaattctt tagtttcaac aatccaattc
     2281 cctgttggtt tttctacttt ttcttttttt aaaaaacctt tattaacaac ctctgttttc
     2341 ttttctctct cagctttaat cttaatttct cctggcaatc gttcgttcat ttccagtaat
     2401 tcatttttct tttcaaaaag ctggttttcc aaagaaagaa tttccttttc taaatccttt
     2461 ttttctttgt attcattcac atctttataa ttatttgaac caactaattt ccgttcgata
     2521 ccacgctctt ttaacatttc ttctaatatc ttcacttctt tttctcgcca atcatcaaaa
     2581 ggacgagttt tatctaattt tggatcttgt tgcgtaattg ctcggtcaaa tgatacttgc
     2641 ttttcaagtc cacgtttata cccgcttgct actggaacaa aattcaaatg catgtgtggg
     2701 ctcgcttcat cattatgaat gactgcgtta taaattttta aatttggatt tcgtttttca
     2761 aatcccaata accattcttc taaaatttcg ttagcctttt caaaatcttg tttactagaa
     2821 aaatcatctt tatcgccaac ttgaataatc atttcttgct gaagtgatgt ttttttactg
     2881 gactgaatgt gtttataata gtcatcaatt tttcggtcgt tacgtttttg tttggcgtta
     2941 tagttctcca acacttctcc aaattcttct cgatataatt tctttaaatc tttctgaaca
     3001 agatatttat tttcgtcaga ccttgaataa tctatgtgcg aattttgctc tttttccttc
     3061 tcgctcattg tcctgttatt gtgtctaatg ttggttttgt tcgttgcctt cttgagagaa
     3121 actgacatac tcataacgaa tcaactcctt ggttccacta taccaaaagc tttgttacct
     3181 tggcaaagta accttttctg tattgtgaca ttccgctacg ctccatgtac acaatgaaaa
     3241 ggctctgcga ggctgttttg gctatcgcca agccatgctt tgcatgacaa aaaacaaaac
     3301 cttggactct gtccaaacct gctggggaac tcttccccag acccccttaa tccaactccc
     3361 caacccctca atccttgagg ggctccctcg ccggttggca gagtcaaaag ggtacctttt
     3421 gacaaccaca agagggttgc atagtgagtg tgtcaactcc ttatcctccc gcccccttac
     3481 gggttcggtc cggtaccgtt gacacacgct atgcagctac ctctacctaa ttaaaaacct
     3541 aattttctac tcaagtagca ctacataggt tcccctctct tttgatttat aaaatcaaca
     3601 ttctatgaac ttgttattaa ggagtaccac cagcattttg aaaaaaacca cgctaagagt
     3661 atgcaagatt ttatgcaaaa aatcggaaga catagggaac tatggggagg ccgaaaagcc
     3721 cgccataata ggaatgtata aaaaaatgca tagaatcata gaaaaagaca aaggcggatt
     3781 ccttgtggga atagggatcc gcctttgtct tcactaccga taatgagacg ttatgttaac
     3841 tgatagtgta tggagtatac aaagaaaac
//
