LOCUS       AP022898                4796 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus MRY14-0075 plasmid pMRY14-0075_4 DNA, complete
            sequence.
ACCESSION   AP022898
VERSION     AP022898.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203326
            Sequence Read Archive: DRR206366, DRR206390
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 316x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0075"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0075_4"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            complement(464..658)
                     /locus_tag="BCM0075_p41"
     CDS             complement(464..658)
                     /locus_tag="BCM0075_p41"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21388.1"
                     /translation="MYDYQSARKQQIQAKIDDLQREIDMLKQEQEDIYDRRKADELQTQ
                     RVFGGIAWDNPVEVARMFR"
                     /name="hypothetical protein"
     gene            complement(670..912)
                     /locus_tag="BCM0075_p42"
     CDS             complement(670..912)
                     /locus_tag="BCM0075_p42"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21389.1"
                     /translation="MMQFKHTEEERQFLQDKIAKLKRENLLGSTLESQVCANEHVIKQL
                     EMFMNQKVCVDEKNVYFNPIKEGLNNPDFRFPYAE"
                     /name="hypothetical protein"
     gene            complement(941..1387)
                     /locus_tag="BCM0075_p43"
     CDS             complement(941..1387)
                     /locus_tag="BCM0075_p43"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21390.1"
                     /translation="MGIGVGTIKKVGSSILSGGKVSKAGIGFGALDVSMNLQNGDDLGT
                     SVAKASVTGALASSNPGLFFLGTAASLGQESYWGLQQFNYQKQQWWNGQYATNNRIGGN
                     YVDTQRALTMRQASVQAIQGSKMNARSALGGEAKILNPYSSRRY"
                     /name="hypothetical protein"
     gene            complement(1378..1656)
                     /locus_tag="BCM0075_p44"
     CDS             complement(1378..1656)
                     /locus_tag="BCM0075_p44"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21391.1"
                     /translation="MQKTTEGIGAVLRQKRMNQLLSLRDLSGILDIAKTLLQKYETGKR
                     NISRNHFPIIVDYLEGRWDQEIMQNRLEGLRQKYEHLTGKRGLEFWE"
                     /name="hypothetical protein"
     gene            2109..2711
                     /gene="res"
                     /locus_tag="BCM0075_p45"
     CDS             2109..2711
                     /gene="res"
                     /locus_tag="BCM0075_p45"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000023352.1"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="BCC21392.1"
                     /translation="MNTMKKFGYVRVSSKEQNESRQKEAMLEQGIAERDIYTDKQSGKD
                     FNREQYQTLKNQLRAGDILYIHSLDRFGRNKDEIEKEWKEITKDIKADIVVLDMPLLDT
                     TKHKDDMGSFVSDLVLQILSWMAEEERKKIKQRQREGIDVALRNGTQFGRPKAEADEKF
                     VTAYNKWKAGEITATKAIEEAGVKRTTFYKLAKGMEE"
                     /name="res"
     gene            2770..3132
                     /locus_tag="BCM0075_p46"
     CDS             2770..3132
                     /locus_tag="BCM0075_p46"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21393.1"
                     /translation="MFNNIKPTATEPWRQLVEVLRYHEKETFQFFIESKDKSLSLSYKI
                     PNMRVSEHEGHEVLGKIISIASDTTEHAHSILTLDKESIINVRSTYFPEEITWRQDGFE
                     IEYKNDIKITFDVLED"
                     /name="hypothetical protein"
     gene            complement(3580..4341)
                     /locus_tag="BCM0075_p47"
     CDS             complement(3580..4341)
                     /locus_tag="BCM0075_p47"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /inference="similar to AA sequence:RefSeq:WP_000121259.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21394.1"
                     /translation="MSIQAYNIKAIVSGGIYEFYRYEDTIFRGFTSTKSSGTVNTYHDA
                     DGNPVILDAQTGELIPKQLNSRAKSNIRARNQLRRLALANFGNKSKFLTLTFKEHINDL
                     EESNKLFKAFIRKLKKEQADLKYIAVIEFTKIGRIHYHVLCNLKYIKAKRIEELWGQGF
                     IKINRINHVDNIGAYIIKYMTKEDADPRLQGKKMYQTSQKLDKPKELVGKQAEYLYEQM
                     LKEKRKKVYSSSYENKQTSNQIIYEEYNLVR"
                     /name="hypothetical protein"
ORIGIN
        1 tttaattatc ggttcattag gagaagaatg ggccacatac accccatctg ggaacccact
       61 ttcaaactct tccatttctt taattaactt ttcacgcttt ctagctgcat gtgctttcgc
      121 aaaaattgca ttgatttctt cccatctgtc ttttttactc attcgaaata cctctctcac
      181 ttatctatca aaacaataga atacaatcgt aagcggacaa ataaaagagg ggtggaattt
      241 ttacacccct agtccgtttc actatccaaa cttcaaataa tcaatcccca accccttatc
      301 caaggggctc cctcgccggt aggcaccccc aaaacgatgt tttggagagc ataaaagggt
      361 cttggtatag tgcaccagtc cactccttat gctcactccg ttccgctacc atggacttat
      421 atcatatatc cgtctctact gtttatcagc tcatgcagta cgtttaacga aacatcctag
      481 ctacctcaac agggttgtcc catgcgatac caccaaatac acgttgagtc tgtaattcat
      541 ccgctttacg tctgtcatag atgtcttctt gctcctgctt caacatatct atttcacgtt
      601 gcaaatcatc tattttggct tgtatttgtt gtttacgtgc cgattgatag tcatacatgt
      661 acatttctcc tattctgcat atggaaaacg aaaatcagga ttattaagcc cctctttaat
      721 tggattgaaa tacacgtttt tctcgtctac acatactttt tgattcatga acatctccaa
      781 ttgcttgatt acatgctcgt ttgcacagac ttgagattcc aatgttgaac ccaataaatt
      841 ttctcgcttc aatttagcga ttttgtcctg taaaaattgt ctttcttctt cagtatgttt
      901 aaattgcatc attttttatt gctccttcag ttagttgttt ttaatatctt ctagatgaat
      961 aaggattcaa tatcttagct tctccaccaa gagcggaacg tgcattcatc ttactacctt
     1021 gtatcgcttg tacagaagcc tgtctcatag taagggcacg ttgcgtatct acatagttac
     1081 caccgattct attatttgtg gcatattgtc cattccacca ctgttgcttt tggtaattga
     1141 actgttgtaa tccccaataa ctctcttgtc ctaagcttgc agcagtcccc aaaaagaaaa
     1201 gacctgggtt cgacgaagcc aatgcacctg ttacagaagc ttttgccact gatgtaccta
     1261 aatcatcacc attttgcagg ttcatcgaaa catcaagggc accaaatcca atacctgcct
     1321 tactgacttt tccacccgaa agaatactgc tcccaacctt tttaatcgtt ccaacgccta
     1381 ttcccaaaac tccaaacctc ttttccctgt cagatgctcg tatttctgtc ttagaccttc
     1441 taaacgattc tgcattatct cttgatccca acgcccttcc aagtaatcaa cgataatcgg
     1501 aaagtgattt cgagagatat ttcgcttacc tgtttcgtac ttttgtaaca atgttttcgc
     1561 tatatctaaa attcccgata aatcacgtaa cgaaagtaat tgattcattc gtttttggcg
     1621 caatactgct ccaattccct ctgttgtttt ctgcatatta ttgctccttt ttcatttgat
     1681 gaaaggaaca taagcatttg ataaggattt gtctacccaa tgggtacgaa gtttccaaac
     1741 ggatacaaat gactcctcga tgagggtcta atagctaaat catgtaaaaa acgcctatat
     1801 agacataaaa aaattgaaaa attttgtgac aggctatttt atatggattt gctttaccgc
     1861 aatcttttcg agcccgcccc gtctatgacg atgtcaaata aaaggagatg agagaaatgt
     1921 agaatccaat attcaatcaa agtttatggt tgttcgtatt tgtatacttt tacgaacact
     1981 taaaaatcat ctaaaaacgg tcgtatttct tgttcgtatt catccgaaaa tactttacga
     2041 atgttcgttt agttgatggg tgttttacga atagaaatga tacaattaaa ttactggagg
     2101 aataaaaaat gaacactatg aaaaaatttg gttatgtacg agtaagtagc aaagagcaaa
     2161 atgaaagcag acaaaaagaa gccatgcttg agcaaggtat tgcagaacgt gacatttaca
     2221 ctgacaaaca aagcggtaaa gacttcaatc gtgaacaata ccaaacatta aaaaatcaat
     2281 taagagcagg cgatatacta tacatacatt ctttggatcg ctttggaaga aacaaagatg
     2341 agatagaaaa agagtggaaa gaaattacta aagatatcaa agctgatatt gttgtactgg
     2401 atatgccatt actagataca acaaaacaca aagacgatat gggttcattt gtatcagacc
     2461 ttgtattaca aatactttca tggatggcag aagaagaacg taagaaaatc aaacaaagac
     2521 aacgtgaagg aatagacgta gctttaagaa acggtacaca attcggcaga ccaaaagcag
     2581 aagcagacga aaagtttgta accgcatata acaaatggaa agcaggagaa atcaccgcta
     2641 caaaagccat agaggaagca ggagtaaagc gcacaacatt ctataagcta gcaaaaggga
     2701 tggaagaatg atgaaagtga gatacaattt ggaagagtgc aatcaatatt aaaaccggag
     2761 gtaatattca tgttcaacaa tatcaaacca actgcaacag aaccgtggag acaacttgta
     2821 gaagtattaa gataccacga aaaagaaaca ttccagttct tcatagaatc caaggacaag
     2881 tctctatctc tatcatacaa aataccaaat atgagagtat cagaacacga aggacatgaa
     2941 gtattaggga aaatcatctc tattgcaagt gacaccactg aacatgctca ttcaatttta
     3001 acgttggata aagaaagcat catcaatgtt agaagtacat actttccaga agaaatcaca
     3061 tggagacagg acggtttcga aatagaatac aagaacgata tcaaaatcac attcgatgtt
     3121 ctagaagatt aagaaccaca ccaacgcatg ttatatcact ctataacgga tatacaggca
     3181 ccgcacatgt cttcgtcacg aatcgtcaat aaagtcgaac acagacgaat ctgaaggata
     3241 tgagccatat tattaggagc catgtagagc cttgtaacaa tttgccgagc atatcgcaaa
     3301 tctaagggca ccgcacacgc aatccaatgt aatagatccc tatgaaatat aatagttcta
     3361 tatagattag aatagattga atggttatat ttatatcaat ccttcagtag tttgattcaa
     3421 cgggcgacac cgtcacttgt cgtcctatac aacttgtaag acataataaa aagagcctgt
     3481 cttatagacg gctcttttct tgtaagttat aattcagtta tctaattagt ttttaaacac
     3541 tcattattga gtatgctttc tagtttgccg actagaaagt tatcttacga ggttgtactc
     3601 ttcgtaaata atttggtttg aggtctgttt gttctcataa gaggatgaat agaccttttt
     3661 tcttttctct tttagcattt gctcatacaa atactccgct tgttttccaa ccaattcttt
     3721 aggcttgtcc aacttttgtg aagtctgata catctttttc ccttgtagtc gtgggtcagc
     3781 atcttcttta gtcatgtatt tgattatata tgccccaata ttatctacat gatttatacg
     3841 gtttatcttt ataaaacctt gtccccataa ttcttctata cgtttggctt ttatatattt
     3901 gagattacac aacacatgat aatgaattct accaatctta gtgaattcaa taacggctat
     3961 atatttcaaa tccgcttgtt ctttctttaa cttccgtata aacgccttaa atagtttgtt
     4021 agattcctct agatcattaa tatgttcttt aaacgtgagc gtaaggaatt tgctcttgtt
     4081 accaaagtta gctaacgcca atcttcttaa ctgattacga gcacgtatgt tgctctttgc
     4141 ccttgaattc aactgttttg gtataagctc accagtttga gcatctaaaa tcacaggatt
     4201 gccgtccgca tcatgatatg tattaacagt tccactactc tttgtagatg taaatcctct
     4261 aaaaattgta tcttcatatc tgtaaaactc atatattcca ccagatacaa tagccttaat
     4321 attgtatgcc tgtatactca ttacatagca gtcctccttc tgcctttcgt tgatgaaaga
     4381 ttattataaa actcacctgc ttatcagctg gctcatttta tatattttga atacaaaaat
     4441 gtaaactttc atttttagac caaacccaac gaaccttaca accccaacgg tttaacagca
     4501 ttgtgggaac acttaatttg gttctttagt gtacgtacaa tcaagttaag tagaccataa
     4561 accccaaacg aagccttctc ctcaataaaa tccgttaaat ctatcatgct acaaatacat
     4621 gaaacttgtc catatgaaac ttaaaacctt cggtcggcct tcggcaaaaa ccccattcct
     4681 catcgtaaag ttttctcatc atcattcgga tacaccaaaa actgttctat ttcttcttct
     4741 gttaaatcat ttggatcaat tcctttctct ctgcaatacc gatacatctc ttttaa
//
