LOCUS       AP022897                6009 bp    DNA     circular BCT 25-DEC-2021
DEFINITION  Bacillus cereus MRY14-0075 plasmid pMRY14-0075_3 DNA, complete
            sequence.
ACCESSION   AP022897
VERSION     AP022897.1
DBLINK      BioProject: PRJDB9236
            BioSample: SAMD00203326
            Sequence Read Archive: DRR206366, DRR206390
KEYWORDS    .
SOURCE      Bacillus cereus
  ORGANISM  Bacillus cereus
            Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Bacillus;
            Bacillus cereus group.
REFERENCE   1
  AUTHORS   Furuta,Y., Miyajima,Y., Maehana,S., Tsujinouchi,M. and Higashi,H.
  TITLE     Genomic sequence of Bacillus cereus isolated from bacteremia
            patients in Japan
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Higashi,H. and Furuta,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2020) Contact:Hideaki Higashi Hokkaido University,
            Research Center for Zoonosis Control; North 20, West 10, Kita-ku,
            Sapporo, Hokkaido 001-0020, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method       :: Canu v. 1.8
            Genome Coverage       :: 316x
            Sequencing Technology :: MinION R9.4 Flowcell; MiSeq
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.5 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Bacillus cereus"
                     /mol_type="genomic DNA"
                     /strain="MRY14-0075"
                     /isolation_source="human blood of bacteremia patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:1396"
                     /plasmid="pMRY14-0075_3"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2013"
     gene            complement(1087..1278)
                     /locus_tag="BCM0075_p31"
     CDS             complement(1087..1278)
                     /locus_tag="BCM0075_p31"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21383.1"
                     /translation="MFFDEANSRENDQIEELKTFSIEESVKMKKEQRNKRNEEFYKQLN
                     ELQKRSDELKQQIKDGRT"
                     /name="hypothetical protein"
     gene            complement(2045..2635)
                     /locus_tag="BCM0075_p32"
     CDS             complement(2045..2635)
                     /locus_tag="BCM0075_p32"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21384.1"
                     /translation="MSKDKVVDFNQAEKNARLRDLENSKILTEDDMKVADELQAKANAR
                     GMKLVPERKIKNRAKFAQLIQDNIDYLEEINYFDNKEIVFLWKISRKVGFLSNCIVDDI
                     HKKSPIPLTQTEIAAVVKRTKNNINPIINSLVDKGILAVSETGLLENNARANAYFMNPN
                     IIFAGNKDDVNLTLRAMFSKIPKELKKLPVKLF"
                     /name="hypothetical protein"
     gene            complement(2913..3371)
                     /locus_tag="BCM0075_p33"
     CDS             complement(2913..3371)
                     /locus_tag="BCM0075_p33"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21385.1"
                     /translation="MNIQKIVEKIETLKMAKIGNEKIKNILELEESVEDIMKKFEEEQK
                     LKTIDEIVEYFNKETKSEDWSYYKVFRLIKKGKLDSTLIKGKHKINIDDAEEFIGESKK
                     TREDYKEECEALKKQIEVLEKKIETLEAKQKKTTTRKRTAPKEEETAK"
                     /name="hypothetical protein"
     gene            complement(4388..4924)
                     /locus_tag="BCM0075_p34"
     CDS             complement(4388..4924)
                     /locus_tag="BCM0075_p34"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21386.1"
                     /translation="MEYGQFAKEVADKVGINPNTLRRWSLELEKLDYEFERNERSQRIY
                     YKADISVLEKMKEFLDKNFSLEESAKQSLEESKKNGVETVTVSEEMNVVATIQERSEEI
                     AIFQEKFVQMVDQQNKIVEQNQQILDLLLEEKREKEEAKREVKFLQDKLDKAIALLQQD
                     EEEKNKKRKKLFGIF"
                     /name="hypothetical protein"
     gene            complement(5055..5699)
                     /locus_tag="BCM0075_p35"
     CDS             complement(5055..5699)
                     /locus_tag="BCM0075_p35"
                     /inference="COORDINATES: ab initio
                     prediction:MetaGeneAnnotator"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BCC21387.1"
                     /translation="MNTKYLCQSWQALVQQVVYLCGRGYHYYHILYLPSHKEDKFLQID
                     KKLINKYQTDTKPYIRSRAKAKGVCNYFYIRYNNVALIMKTDGSIQDGIVEDDQFSDVR
                     YSPMILKISEIVSLSIHYFEKKRLTVKLEKECYETIKGNLLAAANTKNKYKMIKTFNMV
                     NGFPAWAGIIAQKRQLAKYLCAKARKNNVLLEQKELRIVDKRTPVKVWVES"
                     /name="hypothetical protein"
ORIGIN
        1 gcagtagttt gtaatcctta cggattggga cagaatatgg aagcttcccc ttactccccg
       61 ttacaacgta acggatttcg gtacactacg ccttcaaagc actcaaggtc tcgccactcc
      121 aaaatgaatt taccaggttg catccacgac atgactcgtt tcggacgctt tactatgcaa
      181 ctgtgcatag cctttatcgg gttgagctat ttagttacaa gtgttaccac cgcaacctcc
      241 cccgcctgga ccaacgttat aacatttctg ctatcccgca gtatggattc tcaccacttc
      301 gtttataaca aacacaaatc actaagttga ttcgcatttt ttcctagtcg cccataatca
      361 caaataccat cgagtagaag caagctttcg ctcacagccc tcgttgccac aacgcaagta
      421 cgtctccata cacgtcgcga cttccgaata ggttatagta cctcgattgg cggtcgagtt
      481 tttacaccta tccagcacac cccacagacc gtaaactgtg tgtgcacctg caaaaaataa
      541 attagcagat aaatatacat gttcaaattg gcaatttaaa caatttttag tttagtagtg
      601 gcgctactaa actgtggcag tttttaaagc ataatttccc ctttttcgtt agttttttat
      661 taatatcatc tccttttatt tttgttgttt cttcacaaat tttttcgttc caaagaccta
      721 agatcaagac ccaaagatta aaacattaaa agcctaaaaa cacaggtcaa aagaacggtt
      781 ttatgtcagc tccgctgcca tattccttac aagaaattgc aaatgcttta aaacctaaat
      841 ctaccaagct gttcaaataa taatcgaaaa aatcaatttg tgaagaaaaa ttatattttt
      901 cctctcttgc ataaattatt ttgccctatt attttagtaa gctgctttgc caaaaatgcc
      961 tgtctcgtta aaattttcat ttctgaaaga tattctatcg tcagaaacaa taaaaattga
     1021 aatttgagcg ttttttgttc gtttttcaca tatgccatgt ccgttggaca taaaaaaaag
     1081 cacacattaa gtgcgcccat cttttatttg ttgtttcaat tcatcagatc gtttctgcag
     1141 ttcatttaat tgtttataaa actcttcatt tcgtttgttt ctttgctctt ttttcatttt
     1201 gacagattcc tctatgctaa atgttttaag ttcttctatt tggtcatttt cacgagaatt
     1261 tgcttcatca aaaaacataa aagtacctcc caataaagaa aacaacagtt ataaaaaact
     1321 gttgtttccc aatcctagtg tatgcaggcc cccatccact aatattgtat gtaattcaac
     1381 cccaaaaaaa cacctttccc ttgggtttag gttccaagat accaaacaag gtcctgatct
     1441 tcacgagcga agcggactga tcttaaaagc agaatcccga tctttgataa attaatcctg
     1501 tttttttggt ttctgcactt gtgttttagc ggttttccca ttactttttg ggttgaggtt
     1561 ggctcgtagt gtgcggacga aaccgcacgg ttttaaaagg ttttttacac tacatttgga
     1621 gtaagaatat atgtatgcac agcgtttttc gtcgtacaat tggtagccaa gccgataaat
     1681 tgcataaaat cctttttggt atatcggctt ggcaaagcca ttcacacgac gaaaattttt
     1741 tgtgtcaaga acgcaacacg agcttgcgag attgcgaggg tggagatttt ttgcatgttc
     1801 ttcgcaaaaa atactacctg cttctttact caaaaaaagc aaaacaatat atagagtgag
     1861 agagtagtac tttgtaaaag tggtatgaag tattgatatt actggttttt ttggatattt
     1921 cgctgttctt atttttaaga actttagttc tcgattttaa gaacagcaaa aaaagactga
     1981 tatatcaacg tataccagtc tttttccata tttttacttt aaaattattt ttccatattt
     2041 tcacttaaaa gagttttaca ggtaattttt ttaattcctt tggaatcttt gaaaacattg
     2101 ctcgtaatgt taaattaaca tcgtctttgt ttccagcaaa aatgatatta ggattcataa
     2161 aataagcatt tgctcttgca ttattttcaa gtaatcctgt ttcacttaca gcaagaatcc
     2221 ctttgtctac taatgaatta ataattggat ttatattgtt tttagttctc tttacgaccg
     2281 ctgcaatctc tgtttgagtc aaaggaattg gactcttctt atgaatatca tctacaatac
     2341 aatttgataa aaaaccaact tttctactta tcttccaaag aaacacaatc tctttattat
     2401 caaagtaatt gatctcttcg agataatcta tattatcttg tattaattga gcgaatttcg
     2461 ctctattttt aattttacgt tctggaacaa gcttcatacc acgagcattc gctttggctt
     2521 gcaattcatc tgctaccttc atatcatctt ctgttaatat cttagaattc tctaaatcac
     2581 gcagtcttgc atttttttca gcttgattaa aatctacaac tttatcttta ctcatatgta
     2641 ccacatctcc tattttgata aaagtttaac aaaaagggag attactcaca tttttccctg
     2701 caaaaattaa gcaggacata ttgatttttc aaagaatata cattaaagta taaagtagaa
     2761 aataacgttt tggtgagagt aatcacccga caagtgttga cgcacttgtc tttttttttg
     2821 tctaaagtta ctataccgcg tattttcaaa aaataccata caacctataa aaccgacact
     2881 acaccgtatc aaaattgata cggttttgtc gtttatttag ctgtttcttc ttctttaggt
     2941 gcagttcttt ttcttgttgt tgttttcttt tgctttgctt ctaatgtttc gatttttttc
     3001 tctaaaacct ctatttgctt cttcaatgct tcgcattctt ccttataatc ttctcttgtt
     3061 ttcttgcttt ctccaatgaa ttcttcggca tcatcaatgt tgatcttatg ctttcctttg
     3121 attaatgtag aatccaattt tcctttttta ataagtcgaa agactttgta ataactccag
     3181 tcttcactct tcgtttcttt attgaagtat tcaacgattt catcgattgt ttttaatttc
     3241 tgttcttctt cgaatttctt cataatgtct tcaacggact cttcgagttc taagatattc
     3301 ttaatctttt catttccgat ttttgccatt ttcaaagttt cgattttttc gactattttc
     3361 tgaatattca tacttttcat tcctcctaat tttgcaaaac gtattttcca aatttcttac
     3421 tttaaatata actgaattga aaaaatattg caaggttttt gcaaaacttt tattttgcat
     3481 tttacgacaa aacgcaaaaa caatcgtttt gtatttactt caaacacaaa aatgtttttc
     3541 cttatttttt cttttataaa aatataaata caataatttt gcacttcata caaaatttaa
     3601 aaatgaaaaa aatgttgtta aacaaacgtt tataggcttt ttatattttg cattttgcta
     3661 caaaatataa aaaccaactt taatattttg caacaaaacg caaaattaac ttattgcatt
     3721 tattattatt tgcaaaatca atctaatata atttttattc cattgcaatt tttcactttt
     3781 aataaaaata caaaattgca atttgtattt ttatataatt tttcattctt tgcaatttgc
     3841 ttttccttat tacttgcaat ttgtaaaaat aataatttgc acacattgca aaattgtaaa
     3901 atgttaattt ttcatcaatt aaaatttttc attacttgca attttgtatt cattgcaatt
     3961 tatactattt ttcaatttgc aaaaacatgc aaaattactc attttgaaat tttcatcatt
     4021 tgcgatttta taaatcatgc aaaattttca gtgtgtcatt tttgaaaaaa cgtgcaaaat
     4081 taccactttc gattttaaag catttgcaaa atcccaaaaa acgttgaaaa tactggtgtt
     4141 tctcaaatca aaaaatccat ttttcaagaa aaaaatgcac atttccaatt tttaggaaat
     4201 ccgtttgagg cactagaact ggcaaagcct aacaggctaa cagatcgccc tctcacagtc
     4261 aaggcgggaa agagtacctt gacagcgagc ctctagcgct ccgttttcct agccagtgcc
     4321 tcagttcata acgtgccttg atcttgctca aaaataaaaa aaagactatc tttcgatagt
     4381 ccaaaaatta aaaaatccca aataatttct ttcttttttt gtttttttct tcttcatctt
     4441 gttgaagtaa ggcaatagct ttatctagct tatcttgaag gaatttcact tctcttttcg
     4501 cttcttcttt ttcccttttt tcttcaagca gtaaatctaa aatctgttga ttctgctcta
     4561 caatcttgtt ttgttgatct accatttgta cgaatttttc ttgaaaaatc gctatctctt
     4621 ctgaacgttc ctgaattgtt gcgacaacgt tcatttcttc ggaaacggtg accgtttcta
     4681 ctccgttttt cttagattct tcaaggcttt gctttgcaga ttcttctaaa ctgaaatttt
     4741 tatctagaaa ttctttcatt ttctcaagaa cgcttatatc tgctttataa taaatccttt
     4801 ggcttcgctc atttctttcg aactcataat ctagcttttc aagctccagg gaccacctac
     4861 gcagcgtgtt tggattgatt ccaaccttat ccgccacttc ctttgcaaat tgcccgtatt
     4921 ccacccgttt cacccccgtt cttcgaacgt ttctattaag taaatttttc tttattttac
     4981 tttactttac ctttatttta acgcaaaaaa aaagactttg atccataagg acaaaagtct
     5041 attagctata ttgtctatga ttcaacccaa acctttacag gagtgcgttt gtctacaatt
     5101 cgtagttctt tttgttctag aagaacgttg ttctttcttg ctttagcaca taaatacttc
     5161 gctaattgcc gtttctgtgc aataatgccc gcccaagcag ggaagccatt taccatatta
     5221 aatgtcttaa tcattttgta tttattcttc gtatttgcag cggccaataa attgcccttt
     5281 atcgtctcgt aacactcctt ttctagtttg actgtaagtc tctttttctc aaaataatgt
     5341 atactcaaag acactatttc gctgattttt aaaatcattg gcgaatagcg tacgtctgaa
     5401 aattgatcgt cttcaacaat accatcttgt atagacccgt cggtcttcat aatcaatgct
     5461 acgttgttat aacgaatata gaaataatta cacacgccct tcgctttcgc acgagagcgt
     5521 atgtatggtt tagtatctgt ctgatatttg tttattaatt tcttatcaat ctgtaaaaat
     5581 ttatcctcct tatgacttgg taaatacaag atatgatagt agtgataccc cctgccacag
     5641 agatacacca cttgttgtac aagtgcttgc caactttgac atagatattt agtgttcata
     5701 cagatcacct cccgatgaaa aaaaacctag aacacgacag ccatgtcggt taaaaaataa
     5761 ccctgctaat tttttttgct taggaaacac ccttttagcc tagtcacaat gtgaagtgcc
     5821 aacctcacga ctaatgcgaa ttccactacc tcaagcagaa ctcatcactc tccgaagaga
     5881 aacctaatca agtgtgctct caatcctcgc atctcaaatt caaattgggg ccaaccaatt
     5941 ctcctaaaag accgttcgga ccttgcctag ttagtttata aacttcattc atttggtctg
     6001 gtcgctcca
//
