LOCUS       AP022524                3875 bp    DNA     circular BCT 09-OCT-2020
DEFINITION  Enterobacter cloacae STN0717-73 plasmid pSTN0717-73-5 DNA, complete
            sequence.
ACCESSION   AP022524
VERSION     AP022524.1
DBLINK      BioProject: PRJDB9036
            BioSample: SAMD00195991
            Sequence Read Archive: DRR199796
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str. STN0717-73
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 236x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="STN0717-73"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:550"
                     /plasmid="pSTN0717-73-5"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            101..424
                     /locus_tag="STN0717ENT73_P50010"
     CDS             101..424
                     /locus_tag="STN0717ENT73_P50010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48704.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="hypothetical protein"
     gene            1102..1593
                     /locus_tag="STN0717ENT73_P50020"
     CDS             1102..1593
                     /locus_tag="STN0717ENT73_P50020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48705.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="hypothetical protein"
     gene            1600..1815
                     /locus_tag="STN0717ENT73_P50030"
     CDS             1600..1815
                     /locus_tag="STN0717ENT73_P50030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48706.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="hypothetical protein"
     gene            2011..2130
                     /locus_tag="STN0717ENT73_P50040"
     CDS             2011..2130
                     /locus_tag="STN0717ENT73_P50040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48707.1"
                     /translation="MKLLNLIESLLNCLYLAAELWDWLEKHLSVFKALARILV"
                     /name="hypothetical protein"
     gene            complement(2358..2783)
                     /locus_tag="STN0717ENT73_P50050"
     CDS             complement(2358..2783)
                     /locus_tag="STN0717ENT73_P50050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48708.1"
                     /translation="MGSYVDQSLTRNESVISRAQTSWIPTIIPVIIGILLLPFYGLGLL
                     IIIPVLLRVWSTELALTNQRVIAKVGLIRRNTVELRIDKVESLGIHQGILGRIFNYGSI
                     VIKGTGGTNTPIPNIKAPMQFRSIVNNHIEEMGSKQQ"
                     /name="hypothetical protein"
     Typing          3691..3863
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="95.977"
                     /coverage="100.0"
                     /evalue="4.31e-74"
     Typing          complement(3228..3766)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.404"
                     /coverage="92.0"
                     /evalue="9.000000000000003e-166"
     Typing          415..1907
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.6"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gacgcgcctg cggcttgtcc aaccccgcgc cgaccgggaa aggctttccc gcccgtcccg
       61 gcgttatcag gaggccggtt tgtcagagaa acggagcaag atgctcacca tgtgggtgac
      121 ggaggacgag caccggcggc tgctcgagcg ctgtgacggc aggcagctgg cggcgtggat
      181 gcggcagatc tgcctggacg aaaagccggc acgttccgga aagctcccct cactctcgcc
      241 ggcgctgctg cgccagcttg ccggcatggg caataacctg aaccagatag cccgccgtgt
      301 taacgccggc ggcggcaccg gccatgacag ggtacagatt gtggcggcgc tgatggccat
      361 cgatgccggc cttgagcggc tgcggcacgc cgttcttgag aaaggaacgg acgatgatcg
      421 ttaaatttca tccgcgcggc cgcggcggtg gcgcctggcc ggtggactat ctgctgggta
      481 aagaccggca gcgtgaaggc gccaccgtcc tgcaggggaa gccggaagaa gtccgggagc
      541 tgattgacgc gtcgccttac gttaagaaat acacctccgg cgttctgtcg tttgcggagg
      601 ccgatctgcc tcccggccag cgggaaaaac tgatggccag cttcgagcgg gttctgatgc
      661 ccggactcga taaagatcag tacagcattc tgtgggtgga gcacgcggac aaggggcgtc
      721 tggagctgaa ctttctgatc cccaataccg agctgctgac cggcaggcgg ctccagcctt
      781 actacgaccg cgccgaccgg ccgcgcatcg atgcctggca gactatcgtg aacggcagac
      841 tggggctgca cgacccgaac gcgccggaaa accgccgggc gctggtcacg ccgtccgggc
      901 tgccgaaaac gaagcaggag gccgcagaag ccattacccg gggactgctg acccttgctt
      961 cgtccgggga gctgaaaagc cgtcaggatg tcactgacac tcttgaaggt gcaggttttg
     1021 aggtggtgcg caccacgaaa agcagtatca gcattgccga cccggacggg gggcgaaaca
     1081 tccgactgaa gggagccatc tatgagcagt cttttaacgc tggcgaagga cttagagcag
     1141 caatcgaaag cgcagcagca gagtaccggc gagatgctga aagccgcatt cagcgagcac
     1201 gagcggtctg tcagagcgga actgagcgca agcgcgagga gaatcagcga cgccatcctc
     1261 gcccacgagc agagtatgag cgaggcgatg gagaaaaacc ggcggagcgt actgcggact
     1321 gccgggcgga catggctcac catcctgatg gtgtcagcgc tgctgatcgg cacgagcggg
     1381 agcattctgt ggtggcaggg gcagcagata accgacaatt acacgcatct tcgtcagcag
     1441 gaagataccc tggcgaaaat gaccgcccgg acgtggggag tgcgctatca ggagagcagc
     1501 gacggccgga ggtttcttat cctgccgccg gggatgcaaa ccgaagccat cccctacgac
     1561 gggacgacat ggatccgcct gaaacaggag tagcggaaaa tgacagagct ggaaaagcag
     1621 ttgctgagcg cattagagca gctacagcag gactactcgc aaaggctgga cgaatgggag
     1681 agcgccttcg cggaatggcg gagcatgtgt gggcttatgc aacgggagaa cgcggtgctg
     1741 aacgagcgcg tgtcggactt gagcacgcag gtgctgagtt taagcgagca gctgcgccgc
     1801 ttgtccggga actgaatgcc accgagttcc ggcagaggga gaatgagtat cagaaaacac
     1861 tggagctgga gcgccagccg cagaaaacct accgcgggcc gacactttaa accatccagg
     1921 cagctgacaa cccgcacgaa gaggggctct tatttctccc gctaaatagg ttcgtgatca
     1981 aaaaggtcac gacttattca ggagcgggaa atgaagctgt taaatcttat tgaatcattg
     2041 cttaactgcc tgtatctggc ggcggagctg tgggactggc tggagaagca tttgtcggtt
     2101 tttaaggcat tggcgcggat tttagtgtga ctttaacatc catgcatctg gctgcatgac
     2161 tatgcaccag tgtaatattc agcggctgcg tttctggtgt tttttcgtta tgtctgtcgc
     2221 cactttctcg cgtctggcac cggaaacgca gccaaccccg tttcagcggt gcgggcaacg
     2281 gatgttatgg taaattcagc tgattggcag gtacaaaaaa agccccgtag ggcttttttt
     2341 gtaatgctac gaggaagcta ctgctgctta gaacccattt cctcaatatg attgttcaca
     2401 atcgatctga attgcattgg cgccttgatg tttggaattg gcgtgttggt tccgccagtg
     2461 cctttaatca cgatagaccc atagttaaag attcgtccca gtatcccttg atgtatgcca
     2521 aggctttcaa ctttatcaat acggagttcc accgtattgc gtcggatcaa acccactttt
     2581 gcgatgactc tttggttagt taatgcaagc tccgttgacc aaactctaag gagcacaggg
     2641 attattataa gtaaccctaa gccgtaaaaa ggcagcaaca agatcccgat aattactggg
     2701 atgattgttg gaatccagga ggtttgagcg cgagatatta cggattcatt tctcgtcaat
     2761 gattggtcta cgtagcttcc cataaggtta tatccattta gaaaaaatag gatttttccc
     2821 tttttaaggc gagtcagcaa tagaaacttt atgtctccgt ggtaggtata caaaatttac
     2881 ggagaacgta gcgaccgtcg gacagttcgg ttctttcctt tcgtgccgca actggcttga
     2941 ttctgtggtg gtggtggtgg cggctgctat gcctcatata ctcctggcgt tgcaatcctt
     3001 tatcccatat ctgtcgaata tcacagatga tcttcttgag atcctgtccg ttgcgtcgta
     3061 atctcttgct ctgaaaacga aaaaaccgcc ctgcaaggcg gtttttcgaa ggttctcaga
     3121 gctaccaact ctttgaaccg cggtaactgg cttggaggag cgcagtaacc aaatctgtcc
     3181 tttcagttta gccttaacag gcgcataact tcaagactaa ctcctctaaa tccgttacca
     3241 atggctaccg ccactggcga tttttcgtgt ctgttccggg ttggactcaa gatgatagtt
     3301 accgggacaa cgtggtagtc gggctgaacg gggggttctt gcacacagcc cagcttggag
     3361 cgaactgtct aaacggaacg ggacgtggtg atttaggtaa agcttccacc acaacacgga
     3421 tgccgcaggg cgggaacagg agagcgcaag agggagccac cagggggaaa cgcctggtat
     3481 ctttatagtc ctgtcgggtt tcgccaccac tgatttgagc gtcagatttc gtgatgcttg
     3541 tcaggggggc ggagcctatg gaaaaacggc ttcgccgtgg ccttatcgct tccctgttaa
     3601 gcttcttcct ggcatctccc aggaaatttc cgccccgcct gtaagccggt accgctcgcc
     3661 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agtggaatat attctgtatc
     3721 acatattcca cttacgctcc gctgctgcat ttttctcctg ccacatgaag cacttcactg
     3781 atatccgcat ccgtgccaac atagtaagcc agtatacact ccgctagcgc tacgtgactg
     3841 gttcagggct gcgccccgaa acccgccaaa agcct
//
