LOCUS       AP022523                4858 bp    DNA     circular BCT 09-OCT-2020
DEFINITION  Enterobacter cloacae STN0717-73 plasmid pSTN0717-73-4 DNA, complete
            sequence.
ACCESSION   AP022523
VERSION     AP022523.1
DBLINK      BioProject: PRJDB9036
            BioSample: SAMD00195991
            Sequence Read Archive: DRR199796
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str. STN0717-73
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 236x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="STN0717-73"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:550"
                     /plasmid="pSTN0717-73-4"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            101..424
                     /locus_tag="STN0717ENT73_P40010"
     CDS             101..424
                     /locus_tag="STN0717ENT73_P40010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48697.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQICLDEKPARSGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="hypothetical protein"
     gene            1102..1593
                     /locus_tag="STN0717ENT73_P40020"
     CDS             1102..1593
                     /locus_tag="STN0717ENT73_P40020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48698.1"
                     /translation="MSSLLTLAKDLEQQSKAQQQSTGEMLKAAFSEHERSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWLTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESSDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="hypothetical protein"
     gene            1600..1815
                     /locus_tag="STN0717ENT73_P40030"
     CDS             1600..1815
                     /locus_tag="STN0717ENT73_P40030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48699.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="hypothetical protein"
     gene            2011..2130
                     /locus_tag="STN0717ENT73_P40040"
     CDS             2011..2130
                     /locus_tag="STN0717ENT73_P40040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48700.1"
                     /translation="MKLLNLIESLLNCLYLAAELWDWLEKHLSVFKALARILV"
                     /name="hypothetical protein"
     gene            2383..2778
                     /locus_tag="STN0717ENT73_P40050"
     CDS             2383..2778
                     /locus_tag="STN0717ENT73_P40050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48701.1"
                     /translation="MNADEVTRILAQSSQNDWIVDDETGAFTYKNDLALHIKRADYDDY
                     NSFNEPWAVRHPDSNACSVEYTVHYGSSFVDRKTLVSVDGHRATLPMPASATDLNVSSA
                     DVNFAKIVNIGDRVDEYIQRSGLTIKP"
                     /name="hypothetical protein"
     gene            complement(2835..3263)
                     /locus_tag="STN0717ENT73_P40060"
     CDS             complement(2835..3263)
                     /locus_tag="STN0717ENT73_P40060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48702.1"
                     /translation="MSYAIEKKWKAGGLSLGWTAVPTVLFFIQNEKKLSPVAFNTLLNL
                     IVHWWNLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDNRYGGRNI
                     YDLTKLVDYLDIIGPSVSEQVRKPRHKKPIYTVRKKAD"
                     /name="hypothetical protein"
     gene            3548..3976
                     /locus_tag="STN0717ENT73_P40070"
     CDS             3548..3976
                     /locus_tag="STN0717ENT73_P40070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48703.1"
                     /translation="MGSGSSTQNPQLNVVSFSETFNLYYTSRFSQKTLDKIDDFIEHFE
                     KYNLFGDALKGHPAWIGKVSPSWNVPDSYENKEAIEEHARANKLWHAHIGDPVFIDTFH
                     GKYKVSDWVIHFQRLAPNHIKLLELGYHDPMQLPQNFE"
                     /name="hypothetical protein"
     Typing          4673..4846
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="93.714"
                     /coverage="100.0"
                     /evalue="5.6599999999999995e-68"
     Typing          complement(4204..4749)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.091"
                     /coverage="90.0"
                     /evalue="0.0"
     Typing          415..1907
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="96.2"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gacgcgcctg cggcttgtcc aaccccgcgc cgaccgggaa aggctttccc gcccgtcccg
       61 gcgttatcag gaggccggtt tgtcagagaa acggagcaag atgctcacca tgtgggtgac
      121 ggaggacgag caccggcggc tgctcgagcg ctgtgacggc aggcagctgg cggcgtggat
      181 gcggcagatc tgcctggacg aaaagccggc acgttccgga aagctcccct cactctcgcc
      241 ggcgctgctg cgccagcttg ccggcatggg caataacctg aaccagatag cccgccgtgt
      301 taacgccggc ggcggcaccg gccatgacag ggtacagatt gtggcggcgc tgatggccat
      361 cgatgccggc cttgagcggc tgcggcacgc cgttcttgag aaaggaacgg acgatgatcg
      421 ttaaatttca tccgcgcggc cgcggcggtg gcgcctggcc ggtggactat ctgctgggta
      481 aagaccggca gcgtgaaggc gccaccgtcc tgcaggggaa gccggaagaa gtccgggagc
      541 tgattgacgc gtcgccttac gttaagaaat acacctccgg cgttctgtcg tttgcggagg
      601 ccgatctgcc tcccggccag cgggaaaaac tgatggccag cttcgagcgg gttctgatgc
      661 ccggactcga taaagatcag tacagcattc tgtgggtgga gcacgcggac aaggggcgtc
      721 tggagctgaa ctttctgatc cccaataccg agctgctgac cggcaggcgg ctccagcctt
      781 actacgaccg cgccgaccgg ccgcgcatcg atgcctggca gactatcgtg aacggcagac
      841 tggggctgca cgacccgaac gcgccggaaa accgccgggc gctggtcacg ccgtccgggc
      901 tgccgaaaac gaagcaggag gccgcagaag ccattacccg gggactgctg acccttgctt
      961 cgtccgggga gctgaaaagc cgtcaggatg tcactgacac tcttgaaggt gcaggttttg
     1021 aggtggtgcg caccacgaaa agcagtatca gcattgccga cccggacggg gggcgaaaca
     1081 tccgactgaa gggagccatc tatgagcagt cttttaacgc tggcgaagga cttagagcag
     1141 caatcgaaag cgcagcagca gagtaccggc gagatgctga aagccgcatt cagcgagcac
     1201 gagcggtctg tcagagcgga actgagcgca agcgcgagga gaatcagcga cgccatcctc
     1261 gcccacgagc agagtatgag cgaggcgatg gagaaaaacc ggcggagcgt actgcggact
     1321 gccgggcgga catggctcac catcctgatg gtgtcagcgc tgctgatcgg cacgagcggg
     1381 agcattctgt ggtggcaggg gcagcagata accgacaatt acacgcatct tcgtcagcag
     1441 gaagataccc tggcgaaaat gaccgcccgg acgtggggag tgcgctatca ggagagcagc
     1501 gacggccgga ggtttcttat cctgccgccg gggatgcaaa ccgaagccat cccctacgac
     1561 gggacgacat ggatccgcct gaaacaggag tagcggaaaa tgacagagct ggaaaagcag
     1621 ttgctgagcg cattagagca gctacagcag gactactcgc aaaggctgga cgaatgggag
     1681 agcgccttcg cggaatggcg gagcatgtgt gggcttatgc aacgggagaa cgcggtgctg
     1741 aacgagcgcg tgtcggactt gagcacgcag gtgctgagtt taagcgagca gctgcgccgc
     1801 ttgtccggga actgaatgcc accgagctcc ggcacaggga gaatgagtat cagaaaacac
     1861 tggagctgga gcgccagccg cagaaaacct accgcgggcc gacactttaa accatccagg
     1921 cagctgacaa cccgcacgaa gaggggctct tacttctccc gctaaatagg ttcgtgatca
     1981 aaaaggtcac gccttattca ggagcgggaa atgaagctgt taaatcttat tgaatcattg
     2041 cttaactgcc tgtatctggc ggcggagctg tgggactggc tggagaagca tttgtcggtt
     2101 tttaaggcat tggcgcggat tttagtgtga ctttaacatc catgcatctg gctgcatgac
     2161 tatgcatcag tgtaatatcc agcggctgcg ttttcggtgt tttttcgtca tgtctgtcgc
     2221 cactttctcg cgtctggcac cggaaacgca gccaaccccg tttcagcggt gcgggcaaca
     2281 gatgttatgg taaatcaacc gtccgttgat aaaggttctg ataataccca tgtaggtgat
     2341 gttgtttgct ttttatctac acactgcctg atggagctat ttatgaatgc tgatgaagtt
     2401 acacgaattc ttgcgcaatc gagtcaaaat gattggattg tggatgatga gacaggggcg
     2461 tttacttata aaaatgattt agcactgcac attaagagag ctgattacga cgactacaac
     2521 tcgtttaatg agccttgggc tgttcgtcat cctgatagta atgcgtgttc agtcgagtac
     2581 accgttcact atggttcttc gtttgttgat agaaaaacct tggtttccgt cgatggtcat
     2641 cgtgcaacct tgcctatgcc tgcatccgca acggatctaa acgtgtcatc ggctgatgtg
     2701 aatttcgcca aaattgtaaa cattggcgat cgcgttgatg aatacattca acgttctggt
     2761 cttacgatta agccctaatt taacaataag cccggcctcc aaatgcgggc tgttcctgca
     2821 tgtaaccagc ataactaatc ggcttttttc cttactgtat atattggctt tttatgtcgc
     2881 ggctttctta cttgctcgga cacagaagga ccaattatgt ctaaataatc tacaagcttt
     2941 gtaaggtcgt atatgttgcg gccaccatat cggttgtcgc tttttgatgt cggtttttta
     3001 tctaagagat tggctttttc taggtcatta atagcccttt gcaccgtcct tactgacacc
     3061 cccatccgta tagccaggct ctccattgag gggtgcggcc actcctgtag attccaccag
     3121 tgtactatga gattcagcaa ggtattgaat gctacagggc tcaatttctt ctcattttgt
     3181 ataaaaaaca acacagtagg tactgcagtc catcctaatg acagacctcc agccttccac
     3241 tttttttcta ttgcgtaact cattgattat cctgtaaata ttttttttga gatatatata
     3301 atataagtat tcatatgaat agggtgacac cagcgccatt attacacaac tataaaaggc
     3361 taatctgaga acagaaaaac atttctatct gcattaaata cggagttagt gatatgaaaa
     3421 ccaaacaaca tcatgatctt gtaaaagata ctgaaatatt tggtgtatcc ttgtctctgt
     3481 tgatttccag agcgaatgag agatctgtta caatgccatc tggtctaagt agggagcaga
     3541 gaagagcatg ggcagcggat caagcacgca aaatccacaa ttgaatgttg tctctttctc
     3601 tgagacattc aatctttatt acacatcccg cttttctcaa aaaacactcg ataaaatcga
     3661 tgacttcatc gaacatttcg agaagtacaa tctttttggc gatgccttaa aaggccatcc
     3721 ggcttggatt ggcaaggtat cgccatcttg gaacgtgcct gacagttacg aaaacaaaga
     3781 agctattgaa gaacatgcca gggctaataa attgtggcat gcccatatag gcgatcccgt
     3841 tttcatcgat acatttcacg ggaagtacaa agtctctgat tgggtcatcc acttccaacg
     3901 tctagcgccg aaccatataa aactgttaga gcttgggtat catgacccta tgcaactccc
     3961 ccaaaatttc gaatgatctc ttgttcggat gaatgatccc ttgagattcg ttgaccctcg
     4021 tcgtaatctc ttgcggtgaa aacgaaaaaa ccacccggca aggtggcttt ttcgaaggtt
     4081 cgcaagagtt gacgctcttt tgaaccgcgg taactggctt ggaggagcac agtcaccaaa
     4141 actgtccttt cagtgtagcc ttatcgcacc cgaaacttca agactctcct cctctaaacc
     4201 tgttaccagt ggctgctgcc agtggcgctt tgtcgtgttt ttccggattg gactcaagtt
     4261 gatagttacc ggaaaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag
     4321 cttggagcga actgccttcc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc
     4381 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     4441 caccaggggg aaacgcctgg tatctttaag tcctgtcggg tttcgccacc actgatttga
     4501 gcgtcagatt tcgtgatgct tgtcaggggg gcggagccta tggaaaaacg gcttcgccgc
     4561 ggccttatcg tttccctgtt aagtttcttc ctggcatcta ccaggaaatt tccgccccat
     4621 tcgtaagccc gcaccgctcg ccgcagccga acgaccgagc gtagcgagtc agtgagcgag
     4681 gaagcggaat atatcctgta tcacatattc tgctgacgca ccggcgccgc cttttttctc
     4741 ctgccacatg aagcactttc ccgacaaccg cactcatgcc aacataataa gccagtatac
     4801 actccgctag cgctacgtga ctggttcagg gctgcgcccc gaaacccgcc aacagcct
//
