LOCUS       AP022522                4956 bp    DNA     circular BCT 09-OCT-2020
DEFINITION  Enterobacter cloacae STN0717-73 plasmid pSTN0717-73-3 DNA, complete
            sequence.
ACCESSION   AP022522
VERSION     AP022522.1
DBLINK      BioProject: PRJDB9036
            BioSample: SAMD00195991
            Sequence Read Archive: DRR199796
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str. STN0717-73
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 236x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="STN0717-73"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:550"
                     /plasmid="pSTN0717-73-3"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-07-17"
     gene            101..1657
                     /locus_tag="STN0717ENT73_P30010"
     CDS             101..1657
                     /locus_tag="STN0717ENT73_P30010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_011817287.1"
                     /codon_start=1
                     /transl_table=11
                     /product="cell signaling regulator"
                     /protein_id="BBW48692.1"
                     /translation="MRIKKPHLRPIVITLSVGGILLTSIFLIAVIMVFQRENIEKDLLY
                     ANSSYAMKMSDVMSGYIEMAQGELAYGAKKIKSTNDIDSLKDEADRLRLQSGMFNSVLV
                     VSNDAVVLATSPESLDLVGTRLNSSASKLAIEEKKPFISSPLKSVTGNLIILLSHPIYD
                     KGGNYIGYVGGSIYLKKHNLFGDVLSRHFFKNNTEISIVSDDGNVIFNNDKSAVDRPMV
                     MSDDLKSKLTASDRGEGAFFSSGHRYLLGYASMKNTAWNVLVYSRADSVTTILIAYAKS
                     VLVLLIIIIIVFSLISYFIASSISLPLEKLAISTNAKDIGASLVYIKAINAWYAEADRL
                     KKALFTYIKVMMERVDFLNEVALKDPLTGVNNRLGFLSKIQNHKRDSGYSVIVIDVDFF
                     KEINDSFGHGVGDEVLVSLAHIINSCCRSEDVVCRFGGEEFVVFLPNTSVVTAKCVAER
                     IRSVIESTVFPNNLRVTISAGVATQDDPLGRIDFLLKNADDALYQAKREGRNKVIVYSA
                     I"
                     /name="cell signaling regulator"
     gene            1669..1926
                     /locus_tag="STN0717ENT73_P30020"
     CDS             1669..1926
                     /locus_tag="STN0717ENT73_P30020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48693.1"
                     /translation="MLYALVCRFFCGEEWARSGSSCYPFDVVVISYPVNKKTTATNGGS
                     FAGIQSYQLFLPKVTGFHGAQVPKLSFQCSRSQATTSRTL"
                     /name="hypothetical protein"
     gene            2715..3038
                     /locus_tag="STN0717ENT73_P30030"
     CDS             2715..3038
                     /locus_tag="STN0717ENT73_P30030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48694.1"
                     /translation="MLTMWVTEDEHRRLLERCDGRQLAAWMRQTCLDEKPARSGKLPSI
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLGVRLE
                     KGK"
                     /name="hypothetical protein"
     gene            4007..4492
                     /locus_tag="STN0717ENT73_P30040"
     CDS             4007..4492
                     /locus_tag="STN0717ENT73_P30040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48695.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQNTGEMLKAAFSEHEKSVKAELNESA
                     KRISAAILDHDRKLSSAMSQRTKGMVRMVSQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIREQKSTQAMLSERNSGVQLSTCGEQRRRCVKVNPEAGRFGEDSSWMILAGK"
                     /name="hypothetical protein"
     gene            4496..4711
                     /locus_tag="STN0717ENT73_P30050"
     CDS             4496..4711
                     /locus_tag="STN0717ENT73_P30050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBW48696.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWESAFAEWRTMSGLMLRENA
                     ALSERVTGLSRQVQSLSEQLRRLSSD"
                     /name="hypothetical protein"
     Typing          2429..2602
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="98.857"
                     /coverage="100.0"
                     /evalue="5.54e-83"
     Typing          complement(1953..2505)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.741"
                     /coverage="91.0"
                     /evalue="0.0"
     Typing          2716..3032
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="90.566"
                     /coverage="95.0"
                     /evalue="4.2500000000000003e-57"
     Typing          3320..4815
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.182"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 accgggctga cgccgcattt gttccgaacc cgtccggggc ggtgctgata agggatgtta
       61 tggtaaatgc ggcatttttt attatatgga gtgtattttt atgagaatta aaaagcctca
      121 ccttcgtccg atagtcataa ccttgtccgt tggtgggatt ttgttaactt ctatttttct
      181 gattgccgtt attatggttt ttcagcgcga aaatatagaa aaagatttgc tttatgctaa
      241 ttcatcgtac gctatgaaaa tgtcagatgt aatgagtggt tatattgaaa tggctcaagg
      301 tgaattagca tatggagcaa aaaaaataaa atcaactaat gatattgact cacttaaaga
      361 tgaggcggat aggttaagat tgcagtctgg tatgtttaat tcagttcttg ttgttagtaa
      421 tgatgctgtt gttctggcga cttcgccaga atctcttgat ttagttggta cccgcttgaa
      481 ttcaagtgcc agcaaattgg cgattgagga aaagaaacca ttcatatctt caccgttaaa
      541 atcagttact gggaatttga ttattttgtt gtcacatcct atttatgata agggtggcaa
      601 ttacataggg tatgtcggtg ggtctatata tctaaaaaaa cataatttat ttggcgatgt
      661 tttgagcagg cattttttca agaacaacac tgaaattagc attgttagtg atgatgggaa
      721 tgtgatcttt aataacgata aaagtgctgt tgatcggcct atggttatgt ctgatgattt
      781 gaaatcaaag ttaactgcgt ctgatagagg tgagggggca tttttttctt cggggcatag
      841 atatcttctt ggttatgcta gtatgaaaaa tactgcttgg aatgtgttgg tttatagtcg
      901 tgctgacagt gtgactacta ttcttattgc ttatgctaaa agtgtacttg ttctgctaat
      961 tataataatt attgtgtttt cacttatttc atatttcatt gcctccagta tttcacttcc
     1021 tttagaaaag cttgctatat ctaccaatgc taaagatatc ggagcttcgc tagtttatat
     1081 taaagctatc aatgcttggt atgccgaagc tgataggctt aaaaaggcgc tttttacgta
     1141 tattaaggta atgatggaaa gagttgattt tctaaatgag gtggcgctta aagatcccct
     1201 taccggcgtg aacaatagat tggggttttt aagtaaaata cagaatcata agcgggattc
     1261 agggtattca gttattgtaa ttgatgttga tttttttaaa gagataaatg acagtttcgg
     1321 gcatggggtc ggagatgagg tgcttgtttc tttggctcat attattaatt cctgttgccg
     1381 tagtgaagat gttgtttgtc ggtttggtgg cgaagagttt gttgttttcc tgccgaatac
     1441 atctgtggta acagctaagt gtgttgcgga gcgtattagg agtgttattg aaagtactgt
     1501 cttccctaat aacttgcggg tcaccatatc tgctggtgtg gcaacacaag atgatcctct
     1561 tggtaggatc gattttttgc tgaaaaatgc tgacgatgct ctttatcaag ccaaaaggga
     1621 ggggcgtaat aaagtgattg tatatagcgc tatttgagtt ttttgatgat gctttacgcg
     1681 ctagtgtgtc gttttttttg tggcgaagag tgggcaagaa gcggatcctc ttgttatccc
     1741 ttcgatgtcg tcgtaatctc ataccctgta aacaaaaaaa ccaccgctac caacggtggt
     1801 tcgtttgccg gaattcagag ctaccaactc tttcttccga aggtaactgg ctttcacgga
     1861 gcgcaggtac caaaactgtc cttccagtgt agccgcagtc aggccaccac ttcaagaact
     1921 ctgtagaaca ctctactact ccgctccgtg aatcctctta ctaatggcta ccgccactgg
     1981 cgatttttcg tgtctgttcc gggttggact caagatgata gttaccggga caacgtggta
     2041 gtcgggctga acggggggtt cgtgcataca gtccagcttg gagcgaactg ccttcccgga
     2101 accgagtgtc aggcgtggaa tgagataaac gcggccataa cagcggaatg acaccggtaa
     2161 accgaaaggc aggaacagga gagcgcacga gggagccacc agggggaaac gcctggtatc
     2221 tttaagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt gatgcttgtc
     2281 aggggggcgg agcctatgga aaaacggctt cgccgcggcc ttctcgcttc cctgttacgt
     2341 tccttcctgg catcttccag gaaatttccg ccccacctgt aagccggcac cgctcgccgc
     2401 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaatatat cctgtatcac
     2461 atattctgct gacgcgccgg tgccgccttt tttctcctgc cacatgaagc acttcactga
     2521 caccctcatc cgtgccaaca tattaagcca gtatacactc cgctagcgct acgtgactgg
     2581 ttcagggctg cgccccgaaa cccgccaaaa gcctgacgcg cctgcggctt gtccaacccc
     2641 gtgccgaccg ggaaaggttt tcccgcccgt cccggcgtta tcaggagacc ggtttgtcag
     2701 agaaacggaa caagatgctc accatgtggg tgacggagga cgagcaccgg cggctgcttg
     2761 agcgctgtga cggcaggcag ctggcggcgt ggatgcggca gacctgcctg gacgaaaagc
     2821 cggcacgttc cggaaagctc ccctcaatct cgccggcgct gctgcgccag cttgccggca
     2881 tgggcaataa cctgaaccag atagcccgcc gtgttaacgc cggcggcggt accggccatg
     2941 acagggtgca gattgttgcg gcgctgatgg ccatcgatgc cggacttgag cggctgcggc
     3001 acgccgttct tggggtccgc ttggaaaagg gaaaataacc tacgctaaga ctacttttta
     3061 accaatcggc tattggatat cactcggttc gcggggctac agcgtttctg tgtccgaaac
     3121 cgaacgattg cgtacaccat gccaataaca tgtacgaaag taagcaaaac tgttaacgga
     3181 cagtcagatc gttccgtaca tcccttacgg tcaggggttc gcgctcaatg gaacgaaatc
     3241 actcgttaag ctcaaaacgc ttaacgtagg aaaatttccg ttttccaagc ggaccccttc
     3301 ttgagaaagg aacggacgat gatcgttaaa tttcatccac gcggtcgcgg cggtggcgcc
     3361 gggccggtgg actatctgct gggtaaagac cggcagcgtg aaggcgccac cgtcctgcag
     3421 gggaagccgg aagaagtccg ggagctgatt gacgcgtcgc cttacgttaa gaaatacacc
     3481 tccggggtac tgtcgtttgc ggaagcggat ttaccttccg gccagcggga aaagctgatg
     3541 gcaagctttg agcgggttct gatgcccgga cttgataaag accagtacag catcctgtgg
     3601 gtggagcatc gtgacaaggg gcggcttgag ctgaattttc tgatcccgaa cacggagctg
     3661 ctgaccggca gacggctcca gccgtattac gatcgcgccg accgtccacg catcgatgcc
     3721 tggcagacca tcgtgaacgg cagactgggg ctgcacgacc cgaacgcacc ggagaaccgg
     3781 cgggcactgg tcacaccgtc tgcactgcct aaagcgaagc aggaggctgc agaagcgatt
     3841 acacgcggtc tgcttgctct tgcctcatcc ggggagctga aaacccgtca ggatgtcact
     3901 gaggcgctgg aaagcgcagg ttttgaggtg gtccgcacca caaaaagcag tatcagcatt
     3961 gccgacccgg acggggggcg aaacatccga ctgaagggag ccatttatga acagtctttt
     4021 aacgctggcg aaggacttag agcagaaatc gaaagcgcag cagcagaata ccggcgagat
     4081 gctgaaagcc gcattcagcg agcacgagaa atctgtaaaa gcggaactga acgaaagcgc
     4141 gaagagaatc agcgccgcca tcctcgacca cgaccggaaa ctgtcctcag ccatgagcca
     4201 gcgcacgaaa ggcatggtgc gcatggtcag ccagacgtgg ctgaccatcg tcctggtctc
     4261 cgcgctgctg atcgcctcga gcgcgggcat tctgtggtgg caggggcagc agatactcga
     4321 caactacacg accatccggg agcagaagag cacgcaggcc atgctgtcag agaggaacag
     4381 cggcgtgcag ctctcgacct gcggcgagca gagacgccgc tgcgtgaagg tgaacccgga
     4441 agcgggacgg ttcggagagg attcgagctg gatgatactg gcggggaaat agcacatgac
     4501 ggagctggaa aaacagttgc tgagcgcatt agagcagcta cagcaggact actcgaaaag
     4561 gctggacgag tgggagagcg ccttcgcgga atggcggacg atgtctggtc ttatgctacg
     4621 ggagaacgca gcgctgagcg agcgcgtcac gggcttgagc aggcaggtgc agagtttgag
     4681 cgagcagctg cgccggttgt cgagcgactg aatgccgtcg agctgcaccg ggagcaggag
     4741 cgcgtggtgc agcatcagaa agcgctggag ctggagcgat ctcagcgaca gcggacgtat
     4801 aacagccctt cactgtgata acctgataaa acggggcttt cggcggacat ctgcgctgaa
     4861 agccggagcc attcacccga ctgcatagcc atgcagccgg gtgaaattta tctggcgcag
     4921 aatgcgcggt ttagcggaga gtttgctgcc ggtttt
//
