LOCUS       AP022423               12436 bp    DNA     circular BCT 09-OCT-2020
DEFINITION  Klebsiella sp. STW0522-44 STW0522-44 plasmid pSTW0522-44-4 DNA,
            complete sequence.
ACCESSION   AP022423
VERSION     AP022423.1
DBLINK      BioProject: PRJDB9036
            BioSample: SAMD00195884
            Sequence Read Archive: DRR199689
KEYWORDS    .
SOURCE      Klebsiella sp. STW0522-44
  ORGANISM  Klebsiella sp. STW0522-44
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Klebsiella sp. STW0522-44 isolated from
            hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12436)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 360x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..12436
                     /organism="Klebsiella sp. STW0522-44"
                     /mol_type="genomic DNA"
                     /strain="STW0522-44"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:2681387"
                     /plasmid="pSTW0522-44-4"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            101..415
                     /locus_tag="STW0522KLE44_P40010"
     CDS             101..415
                     /locus_tag="STW0522KLE44_P40010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68954.1"
                     /translation="MRTDSLGNTYVTDDNGNQTSYRTDSLGNTYGSDGSMSRTDSLGNT
                     YVTDGNGNQTQYRTDSLGNTYGSDGSSARTDSLGNTYITSPNGDQTSCRTDSLGNTYCN
                     "
                     /name="hypothetical protein"
     gene            complement(466..1149)
                     /locus_tag="STW0522KLE44_P40020"
     CDS             complement(466..1149)
                     /locus_tag="STW0522KLE44_P40020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68955.1"
                     /translation="MPDLKEILKNSLFNQVDDSVSAPISIRLPTVLNNQLEELSQSMKC
                     SKASLITEFIKGGVAVTNELLKEDSLISDELKEQMIAGQREESDVFSLDRKTFMLNTNY
                     NNDEETHFDMLKNQEAAAFCKGWKEYICQLSKGDTVYLYQSGVGIIASGVVDGELVKSE
                     HYGVADDKYAKPLKDFKTGFKAISARRFKELTGGGANFRRTMIELTSMQAHAIAQEIEK
                     LTAKQ"
                     /name="hypothetical protein"
     gene            1309..1779
                     /locus_tag="STW0522KLE44_P40030"
     CDS             1309..1779
                     /locus_tag="STW0522KLE44_P40030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68956.1"
                     /translation="MRYTPEQKEQALTLSRQPGATAGSVSRQMGIPARAIQRWIAADAK
                     QKAAPDNVTSLMEQRERARQMLEETPSAKIRQLRNQFTEQQFTLLQRHATDLTALRSRQ
                     LKAVLDNDPQMTRAVASAILALLKAQEAERQMYDIKPSSEPGIMRRGMNRQN"
                     /name="hypothetical protein"
     gene            complement(2307..3890)
                     /locus_tag="STW0522KLE44_P40040"
     CDS             complement(2307..3890)
                     /locus_tag="STW0522KLE44_P40040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68957.1"
                     /translation="MSISEQDNAVQTEAKPASPLTIALSEVFAGMLSEYLPLHDADKCR
                     TRLQELGVNTQAVTALMRSTLWHTPTKKYMHLKGNEVLDIVQNDFNLLERPGVKLWRCN
                     QAVKDILSEQGVGTVKKIEEFRLKMAKAIREAISGELRYSQQAKQVRLEIDMFAQEPRL
                     KKERGGAVFYLCHTPYGNFDKDGNPAGYDQAIADDYLDHFPTFPAMLDAIVAARFASSR
                     KSAYILLQAISDWGKDFLLGPKGVFASLGVAICLSEKDVIKILSGANSGHLAEEFLGCL
                     MLVINEPTQVTKIQYRLEESLTITSKFLAAVNVPLFTKIFTSADPVPGLSDADGVDSQI
                     ANRFAQVKGDGDIKLRPLFMQDKGHYFDNLCQFTASEINSRIAEYVAMGEKVARKAADD
                     ALGKWHQKHGIANHAGDITASYEQVREAWYAFALDRVVAKHPDFIGFNTVGGFALRSSS
                     ACWTQFVDWYAGKEDGIKRQRLLLDRDVLIGSAKVRQSRSVNGKREGGSTVLLPEIPMS
                     IPDNVKSFKR"
                     /name="hypothetical protein"
     gene            4555..5067
                     /locus_tag="STW0522KLE44_P40050"
     CDS             4555..5067
                     /locus_tag="STW0522KLE44_P40050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68958.1"
                     /translation="MKPKKTASFTLRLDADLLAQIEQLAEQQHRNRTGIITEALRRYVE
                     TSTEGEQEPAGEVQKLDQVIALLGTISGQLGAQAKPAPAVKAERTAPAPQREAPQGVAV
                     HNGDPRFVTISGDADGLIVSMTEQRASLEDIVAALNAAGYRTATGQEWTRSRCRDYRHK
                     LKDLGKL"
                     /name="hypothetical protein"
     gene            complement(5121..5261)
                     /locus_tag="STW0522KLE44_P40060"
     CDS             complement(5121..5261)
                     /locus_tag="STW0522KLE44_P40060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68959.1"
                     /translation="MDAAVKAICGAIRDGLPFEEVRELPEVRTVAEQGLLSLDRLRDWW
                     M"
                     /name="hypothetical protein"
     gene            complement(5263..5400)
                     /locus_tag="STW0522KLE44_P40070"
     CDS             complement(5263..5400)
                     /locus_tag="STW0522KLE44_P40070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68960.1"
                     /translation="MLELFGITDRDPILAQLPHLDALELLTVEDSDKKTIAEAFGEWIK
                     "
                     /name="hypothetical protein"
     gene            5735..5950
                     /locus_tag="STW0522KLE44_P40080"
     CDS             5735..5950
                     /locus_tag="STW0522KLE44_P40080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68961.1"
                     /translation="MSEFWFNALQSYAAFALLFGAAVLMIDFLADKLGIGGPRFPPVMF
                     VPAMLLWPATLGVLIATIAFLINSRK"
                     /name="hypothetical protein"
     gene            5994..6218
                     /locus_tag="STW0522KLE44_P40090"
     CDS             5994..6218
                     /locus_tag="STW0522KLE44_P40090"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68962.1"
                     /translation="MAFTIDDTVNQGSPPGYAPAANVLIVPNYSAGHFLPDGSQQIKTL
                     SATGHTMAPPVSFDFPAPEIFTQDQNARF"
                     /name="hypothetical protein"
     gene            6399..7619
                     /locus_tag="STW0522KLE44_P40100"
     CDS             6399..7619
                     /locus_tag="STW0522KLE44_P40100"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68963.1"
                     /translation="MKRTALVIALTLATTSAMAAPVFYTHPWGNSVEANLNGKTALLND
                     HQDQITQNRVDSESRDTLLGDRITNVDADLQSAKLGAIVINDKADAALAGLNGKVDQAT
                     YDAGQQAQDDHITAVEGAAQTANDRATNLETRADSSEQAIRDTQNAAQTANDRATNLET
                     RADTSEQNIRDVQGAAQIANDRASSLETRADGVEAVNAQQDSTLADHDTRITSNTSALT
                     TKVDKQVFADDQARQDAALKSETTTRTSQVAKLQSGVDQAQATGDYANSRIDVANQNIE
                     ANRQASIATNKRVAANTAQLANHEQRITTLEQNTSARFSDLNSKIDDNRKRASAGIAGV
                     AAMANIPQVLEHQTFAIGAGAGNTDGESALAVGFSARASQNTVVKASVSNDTQHNFVVG
                     AGVAFGW"
                     /name="hypothetical protein"
     gene            complement(7718..7897)
                     /locus_tag="STW0522KLE44_P40110"
     CDS             complement(7718..7897)
                     /locus_tag="STW0522KLE44_P40110"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68964.1"
                     /translation="MRAHAVFMVNQAGPFTCHVVPCQTVCIMPLIHQANFNVTIFLQRS
                     CYVSRFYLPALLIN"
                     /name="hypothetical protein"
     gene            7962..8327
                     /locus_tag="STW0522KLE44_P40120"
     CDS             7962..8327
                     /locus_tag="STW0522KLE44_P40120"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68965.1"
                     /translation="MSKITGAEVRDARIASGLTQQAMADLLGHTLRNYQRKEEGRPDPS
                     GSGEMLSTIMPGEFYFMQSLAIGSLPAIVKTVNSARALLKYTTDLARDPSANPRFVKVV
                     ENMEQALAELEHSKPTR"
                     /name="hypothetical protein"
     gene            8338..8808
                     /locus_tag="STW0522KLE44_P40130"
     CDS             8338..8808
                     /locus_tag="STW0522KLE44_P40130"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68966.1"
                     /translation="MEQNHQPQGEVSNARIAATQLGFDGAAMFGTLQLQAQGFGVGFSG
                     HVLHVEAQTQEQADYQTSFGMDYIAQVLRIVGVQHWESLKGQPVRIAREGNQVIAIGNL
                     IEEIWFEPRKLGEQAQEKAELQAAAPPVPLAWQGLARHPAGPAFAPSPDAAD"
                     /name="hypothetical protein"
     gene            complement(8630..9268)
                     /locus_tag="STW0522KLE44_P40140"
     CDS             complement(8630..9268)
                     /locus_tag="STW0522KLE44_P40140"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68967.1"
                     /translation="MSGNFQKLINMLNAAGGTLTKSMDAADGFPLSKQSLDQALKSCGD
                     MGYGQQTQQLLKDMHAAGLSSGLSDKELEGHIAETMRGLASKRALTKSLTPGDALADAM
                     QSTAAAPQFNPNQITVAPSVEAVHRARAQQVSDAQFLQEVADRGVPFEQVNQLHQVMEQ
                     TRGLPDDVRAPAMQAVRAALPLAARLSPEPARPACEARTRFLRSGCRWR"
                     /name="hypothetical protein"
     gene            complement(9265..9903)
                     /locus_tag="STW0522KLE44_P40150"
     CDS             complement(9265..9903)
                     /locus_tag="STW0522KLE44_P40150"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68968.1"
                     /translation="MSKKTYLAELGERLASEQLLEHPELPQLSLSPDVPAVAAVLNKMR
                     QRSEMEMCLADLESAIPAKQATSQQLRSRIVEAERLIAEAKADLLAGRQPDPNAALQSL
                     IIDDLRQQLIEAQDEQQRAESEISRVRGELSAANAVIDRAMTEYANDVRISRAYALQKA
                     LIEELTSLADADGGRGQYGRKFSVSTSLAAIVNSHGFIEILRTRRGMVS"
                     /name="hypothetical protein"
     gene            10116..10679
                     /locus_tag="STW0522KLE44_P40160"
     CDS             10116..10679
                     /locus_tag="STW0522KLE44_P40160"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000447713.1"
                     /codon_start=1
                     /transl_table=11
                     /product="multiple promoter invertase"
                     /protein_id="BBV68969.1"
                     /translation="MIFGYVRVSTVEQNLGGQIDAILQRYPDARILSDEGVSGITPVME
                     RPGFSALMKVAGSGDVIVVAAIDRIGRNAVSTLEVVEDLKAKGITLISLREGFDATTPA
                     GKLMLTMLAGIAEMERTSMLERQRAGIDSAKKEGKHLGRPIKVTPEIRKKILEKREIGI
                     PAARIARDLNISRASVFRVFKEEN"
                     /name="multiple promoter invertase"
     gene            10775..11011
                     /locus_tag="STW0522KLE44_P40170"
     CDS             10775..11011
                     /locus_tag="STW0522KLE44_P40170"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68970.1"
                     /translation="MPRHCQNQPNGTNIMIIASQRHTAQTFPGLSIEHGQIYQHGKPRS
                     VAMNGRGIHQVSLRTTEGRVLTLPVCRLFEWQA"
                     /name="hypothetical protein"
     gene            complement(11524..11760)
                     /locus_tag="STW0522KLE44_P40180"
     CDS             complement(11524..11760)
                     /locus_tag="STW0522KLE44_P40180"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV68971.1"
                     /translation="MTRLTNLTPAEKKFIDDAIAAAERAAGKKLNQPNRHIVLNRARAQ
                     IESQRYADRQRALREDERQQSDFAWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          complement(11925..12098)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.5600000000000002e-86"
ORIGIN
        1 gtcttgatat ttcaaactgt tagtttctta acgtggtttt tcgcacgggt agccctcgaa
       61 ccccaccttt ggctgcatcg ttaccgtaca ggccagcact atgcgtacag attcgctcgg
      121 taatacctat gtaaccgatg acaacggcaa ccagaccagc tatcgcacag attcgctcgg
      181 taatacatac ggatcagatg gctcaatgtc tcgcactgac agccttggaa acacctacgt
      241 aacagacggg aacgggaacc agacacagta tcggactgac tcattaggga acacttacgg
      301 cagtgatggg agtagtgccc ggactgattc actgggaaat acctacatca catcaccaaa
      361 tggggatcag accagctgcc ggactgacag cctgggaaat acttactgta attaatacta
      421 caaataaaaa gggcccttcc gggccccttt cttatctgag gattatcact gcttagccgt
      481 taatttctca atctcttgag caatagcatg agcctgcata gaggtgagtt caatcatcgt
      541 tctacggaaa ttagcgccgc ccccggttaa ctctttaaag cgacgcgcgg agatagcctt
      601 aaagccggtt ttaaaatctt tgagtggctt agcgtattta tcatcagcta cgccgtaatg
      661 ctcgcttttc accagctcac catccacaac accagaagca atgatgccta cgccactctg
      721 atacagatag acggtatcgc ctttggaaag ctgacagatg tactctttcc atcccttaca
      781 aaaggccgct gcctcttggt ttttcagcat atcgaaatgc gtttcttcat cgttgttgta
      841 gttcgtattt agcatgaaag tttttctatc gagtgagaaa acgtcacttt cctcacgctg
      901 accagcgatc atctgctctt tcagctcatc actaatcagt gagtcctctt ttaatagctc
      961 gtttgtaacg gctaccccgc ctttaatgaa ctcggtaatc agcgaggctt tagagcattt
     1021 catactctgc gatagttctt ctaactgatt gtttaatacg gtaggaagac gaatagatat
     1081 gggtgcagaa acgctatcat caacttgatt aaaaaggcta ttttttaaaa tttctttcag
     1141 gtctggcatg agtttaatct cgctatcata aaaactacat tttgtagttt tgtaagtgta
     1201 atacaatttg tattgtatat caatagccat cggtatggcg gcagctgcgc tgccacacaa
     1261 agcagtcgtg atgccaccat ggggcaccat cccacaggag ccatcaccat gcgttacaca
     1321 ccagagcaaa aagagcaggc cctcaccctc agcaggcaac ccggcgcaac cgctggcagt
     1381 gtctcccgcc agatggggat ccccgcccgt gcgattcagc gctggattgc cgccgatgcc
     1441 aaacagaaag cagcgccgga caacgtgacc agcctcatgg agcagcgcga acgcgcccgg
     1501 cagatgctgg aggagacgcc cagcgcgaag atccgacagc tgcggaacca gtttacagag
     1561 cagcaattca ccctactgca acgccatgcc accgatttaa ccgccctcag aagccgccag
     1621 ctcaaagccg tgctggataa tgacccgcag atgacccgcg ccgtcgccag tgcgatcctt
     1681 gccctgctca aagcgcagga ggcagagcgc cagatgtacg acatcaaacc cagctcagag
     1741 ccaggcatta tgcgacgtgg aatgaaccgc cagaattgac gcattgacgc aacaagtgac
     1801 gcagcacgac agtaaacccc cgccactcct taccccgccg ttgccaaaat gtaaccgtat
     1861 catgaggcac tgagccgccg atctggctcc gggccagtga tttttgcagt gtccgtgcga
     1921 aagcacggca tctaccagta acccccacac ctcagaaccg cgtcacgcag ctgcacaccc
     1981 ctgcggggcg atgcactttg cgacgctatg cggcagctgt taacccctgt taacccctgt
     2041 taacctgcct gttaagcacc taacttactg atatttatat actgttaacc tgttaaccta
     2101 ttttaattaa aagatagtga gtaaaaaaaa gataagaaac gtatttatat atagggagca
     2161 gaggcaaaaa aggttaacag cttaacagtt gccacatttt cattaattat catgcgttta
     2221 tcctgttaat tttcgacgtt aacaggccaa actgttaacc ctcagcgttt aacacccaat
     2281 aaaaaacccg ccgaagcggg ttagtgttaa cgtttgaagc tcttaacatt gtccgggata
     2341 gacatcggaa tctcaggcag cagcacggta gagccgccct ctcgcttgcc gttaacgctc
     2401 cgggactgtc tgaccttggc gctgccaatc agcacatcac ggtcaagcag caggcgctga
     2461 cgtttaattc catcctcttt ccctgcgtac cagtcaacga actgcgtcca gcaagcagag
     2521 gaggagcgaa gtgcaaagcc gccaacggta ttaaagccga tgaagtcagg atgttttgcc
     2581 acaacgcgat ccaatgcaaa ggcataccac gcctcgcgca cctgctcgta actggcggta
     2641 atgtcgcctg catgattggc aatgccatgt ttctggtgcc atttgcccag agcatcatcg
     2701 gcagctttgc gggccacctt ctcacccata gcaacgtatt cagcaatgcg gctgttaatc
     2761 tcggaggcgg tgaactggca gaggttatcg aaataatgcc ccttgtcctg catgaacagc
     2821 gggcgcagtt taatatcccc atccccttta acctgcgcga agcggttagc gatctggctg
     2881 tctacgccgt cagcatcact gaggccggga accggatcgg cagaggtgaa aattttggtg
     2941 aacagcggca cgttaacagc cgccagaaat ttagaggtga tggtcagtga ctcctccaga
     3001 cggtactgga ttttcgtcac ctgcgtcggc tcattgataa ccagcatcaa gcagcccaga
     3061 aactcctccg ccagatgccc gctgttagcc ccgctgagga ttttaatcac atctttctca
     3121 ctcaggcaga tagccacgcc cagcgaggca aaaacgcctt tcgggcccag cagaaaatcc
     3181 tttccccagt cagaaatcgc ctgcaaaagg atgtatgcgg atttgcgaga ggaggcgaag
     3241 cgagccgcca caatggcgtc cagcattgcg gggaaggtag ggaagtgatc cagataatca
     3301 tcggcaatgg cctgatcgta acccgcagga ttgccgtctt tgtcgaaatt gccataaggc
     3361 gtatggcaga ggtagaacac cgccccgccc cgctcttttt tcaggcgtgg ctcctgcgca
     3421 aacatatcaa tctcaagccg cacctgcttg gcttgctggg agtaacgcag ctcaccgctg
     3481 atcgcctcac ggatagcctt tgccatcttg aggcgaaact cctcaatctt tttgacggtg
     3541 cccacgccct gctcgctcag gatgtctttt actgcctgat tacagcgcca gagtttcact
     3601 ccggggcgct ccagcaagtt aaaatcattc tgtacgatgt ccagcacctc attccctttc
     3661 aggtgcatgt atttttttgt cggcgtatgc cagagggttg agcgcataag agccgtgacg
     3721 gcctgcgtgt taacgcccag ctcttgcagg cgagtgcggc atttatctgc atcatgcagg
     3781 ggcaggtatt cactgagcat cccggcgaaa acttcactta gagcgatagt taacgggctc
     3841 gctggtttag cttctgtctg cactgcattg tcttgctcgg aaatactcat tttatcgttt
     3901 ccataactca gagttttttt cgttatggtc gatatagcgg ggcgtcttac gagggaatac
     3961 ggcttgaagg gcgtcaaaat tgacgctaaa ctagcagttg agtaaccgac caacccaagg
     4021 taaagttatc tcttagtatt cccccggaat gatacgcctc acgcatcgac cggaatgcac
     4081 agaaggctcg aaccttctaa gcgattaaaa accagctgcc cggctggttt tttttcgcct
     4141 gtagtctgcc ccgttcctta agcgcagcag acaacatgca gccaatagta tggcgtcgaa
     4201 atgcgatcca aagcaagccc gcagatcttc atctacgtca catgcagcgc tgttaattgc
     4261 gtggataagt ttttgtactt gcccacaatt tcaccctccc cagtggataa gccagagccc
     4321 gcaagcgtgc cgcgccgcac catgggcgct aatggctgga attacggcgc tcatgccgct
     4381 gctgctctcc gggcctgtcc tcgtccgtgc cgaaaggcgc ggcccctacc gctcaccctt
     4441 cgcaaatttg cagtgcattg cattgcattg cggtacatgt gacgcgcttt gcatgacgta
     4501 tgcgccgcgt ttatactgca ttacaccgca ctgcaccgcg aatgagggac aaccatgaaa
     4561 ccaaagaaaa cggcgtcgtt tacgctgcgt ctggatgccg atttgctggc ccagattgag
     4621 cagctggcag agcagcagca ccgtaaccga acgggcatta tcactgaggc gctgcggcgc
     4681 tacgtggaga ccagcacgga gggcgagcag gagcccgctg gagaggtgca gaagctggat
     4741 caggtcattg cattgctggg caccatatcg ggccagctgg gcgcacaggc gaagccagcc
     4801 ccggcagtaa aagcagagcg caccgcacct gcaccacaaa gagaggcacc gcagggcgtt
     4861 gcggtgcata acggtgatcc gcgtttcgtc accatatcgg gcgatgcaga cgggctgata
     4921 gtcagcatga ctgagcaacg ggccagcctt gaggatattg tggcggcact gaatgctgcc
     4981 gggtatcgca cggcgaccgg gcaggagtgg acgcgcagcc gctgccggga ctatcggcat
     5041 aaattaaagg atttggggaa gctctagcgg taggctgcgg cgggggccgc aaaaaaagaa
     5101 agggccgtaa tggcccatta tcacatccac caatcccgga gcctatcgag gctgagcagc
     5161 ccctgctccg cgaccgtgcg aacttccggc agctcccgaa cctcctcaaa gggcagtccg
     5221 tcacgaattg ccccgcatat ggcctttaca gctgcatcca tatcacttga tccactcacc
     5281 gaacgcctcc gcgatggtct ttttgtcgct atcctctacc gtcagcagct ccagtgcatc
     5341 gaggtgcggc agctgggcca gaatcggatc gcggtcagtg atgccgaaca gctccagcat
     5401 tagggcagtg gcgagcgctt cacgctccgc atcgcagaca ccgacaaaag accgttcacg
     5461 gcggtgaatc tgcttaaagg ccagcgcttt gcgggcatcg gcggcaaatt gtggcgtgcc
     5521 gccccagatg gcgttggtaa agctggcccg gtcgataacg tacagctgcc cggtatgggg
     5581 ctggcttttt catcaccctg ctgctgggcc tgattaagtg gtttccacgg atgcacccga
     5641 agcgcaaagc cattggctac ctgctggcgc aggccgtact ctggcccatt accgcatatg
     5701 tggcgctgtc gttctggtat gaggagccat ggcaatgagt gaattttggt ttaacgcgct
     5761 gcaatcctac gcagcgtttg ccctgctgtt tggcgctgcg gttctgatga ttgactttct
     5821 cgcggataag ctgggtatcg gtggcccgcg cttcccccca gtgatgttcg tcccggcgat
     5881 gctgctctgg cctgccactc tgggcgtgct gatagccacc atcgctttcc tgatcaactc
     5941 ccgcaagtag tgacggcata ctggagccct cagacataac gagggctcta cttatggcat
     6001 tcactatcga cgacacggta aaccaaggct ccccaccggg ttacgcgcca gcggcaaacg
     6061 tgctgatcgt cccgaactac agcgccggac acttcctgcc agatggctca cagcagatta
     6121 aaacgctgag cgctaccggg cacacaatgg caccgcctgt atcgtttgac ttcccggcac
     6181 ctgaaatctt cacgcaagac cagaacgccc gcttttaata ttgaccccca aagcaccggg
     6241 ggttaaataa ataccctttc caatcagtca cctgaatcat tcccgcaaat aaatcataaa
     6301 aaagtatcat tgcgctattt ctgacgcgtc aactatgacg tatattctcc tcgtcaacac
     6361 aaacaaaacc gataaaccac caaatgatga ggctttacat gaaacgaact gctttggtaa
     6421 ttgcgcttac gctggctacc acctccgcaa tggctgcgcc tgtgttttac actcatccat
     6481 ggggtaacag cgtcgaagcc aacctgaacg gcaaaaccgc tctcctgaac gaccaccaag
     6541 accagatcac gcaaaaccgc gtggattctg aaagccgcga taccctgctg ggcgaccgca
     6601 tcaccaatgt ggatgccgac ctgcaaagcg cgaagctggg cgctatcgtc atcaacgata
     6661 aggccgatgc tgcactggct ggattgaatg gcaaagttga ccaggctact tacgatgcag
     6721 gccagcaggc gcaggatgac catatcaccg ctgtagaagg tgcggcacaa acggctaatg
     6781 accgtgcgac caatctggaa acccgcgctg attcgtccga gcaggcgatt cgtgatacgc
     6841 agaacgctgc gcagactgcg aacgaccgcg ctactaacct cgaaacccgc gccgatacct
     6901 ctgagcagaa catccgtgat gtgcagggtg ccgcgcaaat cgcaaatgat cgcgcctcgt
     6961 cactggaaac tcgcgccgat ggcgtggaag cggtgaacgc gcagcaggat tcgactctgg
     7021 cggatcacga tacccgcatc accagcaata ccagtgcgtt gactaccaaa gtcgataaac
     7081 aggtatttgc tgatgaccaa gcccgtcagg atgctgcgct gaaaagcgaa acaaccacgc
     7141 gcacttcaca ggtagctaaa ctgcaatccg gcgtggatca ggctcaggct actggtgatt
     7201 acgctaactc tcgcattgat gttgccaatc agaacattga agcgaaccgc caggcatcca
     7261 tcgcaaccaa taagcgcgtc gcggctaaca cggcacagct ggctaaccat gagcaacgca
     7321 tcaccacgct ggagcagaac accagtgcgc gtttctctga cctgaatagc aagattgatg
     7381 acaaccgcaa acgtgcgtca gcaggtatcg caggcgtcgc ggcaatggcg aatatcccgc
     7441 aggtactgga gcaccagacc tttgcaatcg gcgctggcgc tggtaacacc gatggcgagt
     7501 ctgcgctggc tgtaggcttc tccgctcgcg cttctcagaa caccgttgtg aaggcgtccg
     7561 tatcgaacga cacccagcat aactttgtgg taggcgctgg cgtggcattt ggatggtaag
     7621 aaaatcagca agaaccaaag gggctacggc ccctttccca ctgtctgaat taaaatcact
     7681 gctgcattac gatgaggcga cgggaatatt tacgtggtta gttgatcaaa agcgcaggca
     7741 agtaaaatct ggagacatag caggaacgct gcaaaaaaat ggttacattg aaattggcct
     7801 gatgaatcaa cggcattatg cacaccgtct ggcatggtac tacgtgacag gtgaatggcc
     7861 ccgcctgatt gaccataaag accgcgtgcg ctcgcataac tggttctcta acttacgaaa
     7921 agccacccct tcagaaagcg ttgcctaagc aggaaaaacc aatgagcaaa atcaccgggg
     7981 cagaagtccg cgacgcacgt atcgcgtccg gtctcaccca gcaggcaatg gctgacctgc
     8041 tgggccatac cctgcgcaac taccagcgca aagaagaagg ccgaccggat cctagcggca
     8101 gcggcgaaat gctgtctacc atcatgccgg gcgagtttta tttcatgcag tctctggcta
     8161 tcggctcact gccagccatc gtgaagaccg ttaattcggc tcgcgctctg ctgaaataca
     8221 ccaccgatct ggcccgtgat ccgagcgcta acccgcgctt tgtgaaggtc gtcgaaaaca
     8281 tggagcaggc gcttgccgag ctcgaacact ccaaacctac ccgataagga tttacccatg
     8341 gaacagaacc accagccgca gggtgaagtc tccaacgcac gtatcgcagc cacccagctg
     8401 ggctttgacg gcgctgcaat gttcggcacc ctccagctgc aagctcaggg ctttggcgtc
     8461 ggattcagtg ggcacgtact gcacgtcgaa gcgcagacgc aggagcaggc cgactaccag
     8521 accagcttcg gcatggacta catcgcgcag gtgctgcgca tcgtcggcgt gcagcactgg
     8581 gagagcctga aaggtcagcc cgtccgcatc gcccgtgaag gcaatcaggt tatcgccatc
     8641 ggcaacctga tcgaagaaat ctggttcgag cctcgcaagc tgggcgagca ggctcaggag
     8701 aaagccgagc tgcaagcggc agcgccgccc gtaccgcttg catggcaggg gctcgcacgt
     8761 catccggcag gccccgcgtt tgctccatca cctgatgcag ctgattgacc tgctcgaacg
     8821 gcacgccgcg atctgccacc tcttgcaaaa actgggcatc actgacttgc tgcgctcgcg
     8881 cacggtgtac ggcctcgact gatggggcaa cggtgatctg gttagggtta aattgcggcg
     8941 cagctgcggt gctttgcatt gcatccgcca gcgcatcacc cggcgtcagg cttttggtta
     9001 acgctcgttt actggcaagg ccgcgcatag tctccgcaat atgcccctcc agctctttgt
     9061 cactcaggcc ggaactgagg cccgccgcgt gcatatcttt gagcagctgc tgcgtctgct
     9121 gcccgtagcc catatcaccg cagctcttga gggcctgatc gaggctttgt ttgctcagcg
     9181 ggaagccgtc agcggcgtcc attgatttgg tcagcgtgcc acccgccgca ttcagcatgt
     9241 tgatcagctt ttggaaatta ccgctcatga caccatcccc cggcgagtgc gcaggatctc
     9301 aatgaagcca tgcgaattga caatagccgc cagcgaggtg ctgacggaga atttacgccc
     9361 gtactgacca cgcccgccgt ctgcatccgc cagagaggtg agctcctcga tcagcgcttt
     9421 ctggagggca taggcgcggc tgattcgcac atcgttggcg tattcggtca tggcccggtc
     9481 aatcacggcg tttgcggcgg agagctcgcc acgcacgcgg ctaatttcac tctccgcacg
     9541 ctgctgctcg tcctgcgcct caatcagctg ctggcgcagg tcatcaataa tcaggctttg
     9601 cagggcggca tttggatcag gctggcgacc agccagcaga tcggctttag cctcagcaat
     9661 caggcgctcc gcctccacaa tacggctgcg cagctgctgg gatgtcgctt gcttggcagg
     9721 aatggcagat tccagatctg caaggcacat ttccatttca gagcgctggc gcattttgtt
     9781 gaggacagcc gccacagcgg gcacatccgg ggagagactt aactgcggga gctccgggtg
     9841 ctcaagcagc tgctcagaag ccagacgttc gcccagctcc gcgagatagg ttttcttgct
     9901 catcggtttt ttctctttta gtcagcggaa tgctgcccgg acagtttgcc gtcacgactt
     9961 gagcggtagg gggcgtgccg ttggctcgca taccgtcaaa aaggcatccc ttatgcgacg
    10021 ccccaattag aggggcttta cgatgatttg agattgcttt atgcgtctaa agcgtccatt
    10081 atcttttaaa tagacctttt tagacggtaa cgataatgat ttttggctat gtacgcgtat
    10141 caaccgtaga gcagaacctt ggcgggcaaa tagacgccat cctgcaacgc tatcccgatg
    10201 cacgaatcct gagtgatgag ggagtgagcg gcatcacgcc cgtaatggag cgccccggat
    10261 tctccgccct gatgaaggta gccgggagcg gtgacgtgat cgtggtggcg gctatcgacc
    10321 gtataggacg taatgccgtc agcacgctgg aagtggtgga agacctgaaa gcgaagggca
    10381 tcaccctgat cagcctccgg gaaggattcg acgcaacaac cccagcgggc aagctcatgc
    10441 tcaccatgct ggcggggatc gcggaaatgg aacgcacctc gatgctggaa cgtcagcggg
    10501 ccgggattga ttcagcgaag aaagagggca agcaccttgg caggccgata aaggtcacgc
    10561 cggagatacg caaaaagatt ctggagaagc gggagatcgg catacctgct gcacgtatcg
    10621 ccagagattt gaacattagc cgggccagtg tgttccgcgt gttcaaagag gagaattaag
    10681 agcgacgggc cagactggga aggcgtggcc cgtctaagag attggaaccc ctgcgctaac
    10741 aggggaacca aaagcggcgt ggagtcctcg caagatgcca cgccattgtc agaaccaacc
    10801 caatggaacc aacattatga ttattgcatc acagcgtcat acggcgcaaa ccttccccgg
    10861 actcagcatt gagcatgggc agatctacca gcacggcaaa ccccgcagcg ttgcgatgaa
    10921 cgggcgcggc attcatcaag taagcctcag aactacagag gggcgggtgc tgaccctgcc
    10981 cgtctgccgc ctgttcgagt ggcaggcata acaaccgggc cccttcgggg gcctatatga
    11041 taatcaatat ggtgtgacgg attatttatt atgaaaatca atataccttt gctgtttgct
    11101 ttaaccttgg ggttttaagt ccagtggtgt gaaaaaccac gctaagcggt atcagaaatc
    11161 gattttgata cgatccagtc tcaaaatgtc caataaccga tatttggaag atcttttccg
    11221 ccccaaacac atagcctcat aagtcttgat atttcaaact gttagtttct taacgtggtt
    11281 tttcgcacgg gtagccctcg aaccccatta actaccaata tttaccataa tgtctcatta
    11341 tcagcacccc ctgacaggcc ctcagcgcaa ttctggcgca tcagagccgt ttccggcaca
    11401 tttcctcacc cgcatcccgg aaaaacgccc ctgatgattt ctgcacgact gcatggctat
    11461 gcacgcgggt gaatcgcagg tgatttcgca gcgtcagcac cactaaaccg ccaaaatccg
    11521 caatcagcgg cgcggcgcgc gcggacgtga ccaggcaaaa tcggactgct gccgctcatc
    11581 ctcacgtaat gcacgctggc ggtcggcgta acgctgcgac tcaatctgcg cacgcgctct
    11641 gttcaggacg atgtgccggt taggctgatt gagcttcttc ccggccgcac gttcggcagc
    11701 ggctatagcg tcgtcgataa actttttctc agcgggggtc aggtttgtca gtctcgtcat
    11761 gtttatctcc gtatgagaat agtccctgca gtctgccaga cacagtagga acgatgaagc
    11821 gtaagcgacg gtgaaaggca gtcagaaacg gtgaacggga gcggatttta atccggtgcc
    11881 gtgaggcgtt gggcaagccg caggcgcgtc agtgctttta gcgggtttcg gggcgcagcc
    11941 ctgaaccagt cacgtagcgc tagcggagtg tatactggct taatatgttg gcacggatgc
    12001 ggatgtcagt gaagtgcttc atgtagcagg agaaaaaggc ggcaccggcg cgtcagcaga
    12061 acatgtagta acgggagatg tgccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    12121 cggctgcggc gagcggtacc ggcttacggg tgaggtggaa atttcctggt ggatgccagg
    12181 aaggaactta acagggaaac gatgaggctg cggcgaagcc gtttttccat aggctccgcc
    12241 cccctgacaa gcatcacgaa atctgacgct caaatcagtg gcggcgaaac ccgacaggac
    12301 taggggtttt aagtccagtg gtgtgaaaaa ccacgctaag cggtatcaga aatcgatttt
    12361 gatacgatcc agtctcaaaa tgtccaataa ccgatatttg gaagatcttt tccgccccaa
    12421 acacatagcc tcataa
//
