LOCUS       AP022388               10123 bp    DNA     circular BCT 09-OCT-2020
DEFINITION  Citrobacter freundii STW0522-19 plasmid pSTW0522-19-4 DNA, complete
            sequence.
ACCESSION   AP022388
VERSION     AP022388.1
DBLINK      BioProject: PRJDB9036
            BioSample: SAMD00195860
            Sequence Read Archive: DRR199665
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Citrobacter freundii str. STW0522-19
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 364x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="STW0522-19"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:546"
                     /plasmid="pSTW0522-19-4"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            102..683
                     /locus_tag="STW0522CIT19_P40010"
     CDS             102..683
                     /locus_tag="STW0522CIT19_P40010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38515.1"
                     /translation="MRQNSRAAPVAFFHRLRPPARVHINRRFSGASVGAVRLNHESDST
                     GETRQDLKIPTVSGGSLPLALSCSDPAVYRIPVPPFSLTGSVALSHSSHTGISARCRSF
                     APSWAVSKNSPFSPTAAPYPVTVHLSPTRKSTVKRHWQQPLVTGSSQRICLAKHAVALE
                     VCAKVRLHWKDRFGCCALRKPVTTVKQFPN"
                     /name="hypothetical protein"
     gene            complement(786..935)
                     /locus_tag="STW0522CIT19_P40020"
     CDS             complement(786..935)
                     /locus_tag="STW0522CIT19_P40020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38516.1"
                     /translation="MKKAKAIFLFILIVSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="hypothetical protein"
     gene            complement(1020..1277)
                     /locus_tag="STW0522CIT19_P40030"
     CDS             complement(1020..1277)
                     /locus_tag="STW0522CIT19_P40030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38517.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKPWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="hypothetical protein"
     gene            complement(1287..2972)
                     /locus_tag="STW0522CIT19_P40040"
     CDS             complement(1287..2972)
                     /locus_tag="STW0522CIT19_P40040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38518.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSFSGDALSSAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPALQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNITTLNGRITSTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNAKLKAAQASLNAMNDALSRDKAALSGAMESRKQKEKKAKEAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="hypothetical protein"
     gene            3299..3544
                     /locus_tag="STW0522CIT19_P40050"
     CDS             3299..3544
                     /locus_tag="STW0522CIT19_P40050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38519.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            3666..4232
                     /locus_tag="STW0522CIT19_P40060"
     CDS             3666..4232
                     /locus_tag="STW0522CIT19_P40060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38520.1"
                     /translation="MDKRTTLVALVSIIFFTSGCDDQKKGNGNAESTQITKNDITTQLK
                     LDKTQQPDDIEKKIKEIQANSTPEKIKKFNELKDDDPKILRESVVSEIKKKLPLLVDEA
                     TLMTDVSTDGGTFSYKYVTKGISASTMESDVWKDAMQKNIKNSYCSDDARLKVFRELFP
                     EGVIYNYYLSDKLIYTYKALPSICS"
                     /name="hypothetical protein"
     gene            4318..4581
                     /locus_tag="STW0522CIT19_P40070"
     CDS             4318..4581
                     /locus_tag="STW0522CIT19_P40070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38521.1"
                     /translation="MAAYTSETAKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGNDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="hypothetical protein"
     gene            4927..5190
                     /locus_tag="STW0522CIT19_P40080"
     CDS             4927..5190
                     /locus_tag="STW0522CIT19_P40080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38522.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRSGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     gene            5180..5479
                     /locus_tag="STW0522CIT19_P40090"
     CDS             5180..5479
                     /locus_tag="STW0522CIT19_P40090"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38523.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHILYDVDDQARKVRVLAVVGQRQNPHTVS"
                     /name="hypothetical protein"
     gene            complement(5541..5918)
                     /locus_tag="STW0522CIT19_P40100"
     CDS             complement(5541..5918)
                     /locus_tag="STW0522CIT19_P40100"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38524.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETQERDGLRLPGETEASVSEALISEIKALREEVAGLRQEMQAMRLLEH
                     KPETADTHPETSRRWWHWGKR"
                     /name="hypothetical protein"
     gene            5987..6166
                     /locus_tag="STW0522CIT19_P40110"
     CDS             5987..6166
                     /locus_tag="STW0522CIT19_P40110"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38525.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="hypothetical protein"
     gene            complement(6194..6724)
                     /locus_tag="STW0522CIT19_P40120"
     CDS             complement(6194..6724)
                     /locus_tag="STW0522CIT19_P40120"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38526.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLSDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPSGKK"
                     /name="hypothetical protein"
     gene            complement(6731..7462)
                     /locus_tag="STW0522CIT19_P40130"
     CDS             complement(6731..7462)
                     /locus_tag="STW0522CIT19_P40130"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38527.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     gene            complement(7462..9423)
                     /locus_tag="STW0522CIT19_P40140"
     CDS             complement(7462..9423)
                     /locus_tag="STW0522CIT19_P40140"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV38528.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGSAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPNFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QPEARRQRPGDDDFAGLY"
                     /name="hypothetical protein"
     BGC             1..7972
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          103..683
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(6735..7462)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.473"
                     /coverage="100.0"
                     /evalue="7.29e-144"
ORIGIN
        1 cgctcactcg gtcgctacgc tccgggcgtg agactgcggc gggcgctgcg gacacataca
       61 aagttaccca cagattccgt ggataagcag gggactaaca tgtgaggcaa aacagcaggg
      121 ccgcgccggt ggcgtttttc cataggctcc gccctcctgc cagagttcac ataaacagac
      181 gcttttccgg tgcatctgtg ggagccgtga ggctcaacca tgaatctgac agtacgggcg
      241 aaacccgaca ggacttaaag atccccaccg tttccggcgg gtcgctccct cttgcgctct
      301 cctgttccga ccctgccgtt taccggatac ctgttccgcc tttctccctt acgggaagtg
      361 tggcgctttc tcatagctca cacactggta tctcggctcg gtgtaggtcg ttcgctccaa
      421 gctgggctgt aagcaagaac tccccgttca gcccgactgc tgcgccttat ccggtaactg
      481 ttcacttgag tccaacccgg aaaagcacgg taaaacgcca ctggcagcag ccattggtaa
      541 ctgggagttc gcagaggatt tgtttagcta aacacgcggt tgctcttgaa gtgtgcgcca
      601 aagtccggct acactggaag gacagatttg gttgctgtgc tctgcgaaag ccagttacca
      661 cggttaagca gttccccaac tgacttaacc ttcgatcaaa ccacctcccc aggtggtttt
      721 ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
      781 cttttctact gaaccgcgat ccccgtcagt tcagaagagg acgatggtgc caccgttcca
      841 ccctgaacat cccggatata gtttgcctga catgcgacca gaagaaagcc tgaaactatc
      901 aatataaaaa gaaaaatagc ctttgccttt ttcatatacc ttccttgtat tcagaggctt
      961 cggcattcat ccgtgaacac cggaagcctg tatttcaact catccttgat aacttttgct
     1021 taccagttac catcccggta aacaaaggat acaaagtaat caaatttact aatatcgatt
     1081 acatttttaa agtgtggctg taatatcgaa acccatggtt tttcaacatc aaaccaacca
     1141 ttattaatat tatcctttaa aggcatcccc agactttcaa tgacagaacc atcatcaccg
     1201 aagtcttttg agtattcacc gcctttaaac tcttcggttt tcttatcaaa ccaatgaata
     1261 tgtaatttaa gccccatatt tacctcttaa agatattttt taatgtttcg ttttggatcc
     1321 ggccctttaa cctgcttacc cgtttttgga tcgaatgcgc cgaggtgttc gccatcactg
     1381 gcgcggtatc cttcaagctc accgtgctgg gaatcccatt cataaatttt acgcttttta
     1441 tcaccatacc atcgggcccg cttaccacct cctccttgtt taggggtttt aggttttccc
     1501 tctttcaact ctcccaaccc cttaatatct tcagtcttgg gatcaggaaa ataatcatgg
     1561 ccataatctt tagttccctt gcgaggcttt ttcttttcct catttaattt attttctgcc
     1621 tccttcgctt ttttctcctt ttgtttccgg ctctccatcg ccccggaaag cgcagcctta
     1681 tctctggata aggcatcatt cattgcgttt aaagatgcct gagcagcttt taatttcgca
     1741 ttctgattct gaatatcctt atcaagatag cgaacctgat cctgccaccc ggagccatat
     1801 tcatgaggag ggttataagt cacaaaatca tccttattcg cctccgcctc tttcaccttt
     1861 ttatcagctt cctggacagc agccctggcg ccaggaatcg ctttttctgt cgatgtaatc
     1921 ctgccgttta aagtcgtaat atttttatct tcggcatcca gctcagcttt cgctttatca
     1981 tattctcttt tcagcccctc ttccggatga gcggcgtccc atgcctgctg gcgacgcttt
     2041 tcttcctcct gacgctgttt tacctgtgcc ggggtaagaa catctgtcac cgacacatag
     2101 accggcttct gtccggtctc tttcgggaaa cgaataacgg cctcacggga gttaccaccg
     2161 gccgtaaaac cagccggacg tgaatcacct ttttcagcaa caatgccttt tggcggggct
     2221 ttcgctgccg gaacaccttt cggtacgctc acctgcagag ccgggagacc cggaatagac
     2281 acactgaata cccccggacg ttttgtcggt ttcgcatcca ccacaggaac actcattggc
     2341 cgacccgcga caaccgcaat atgctgccgc tcatccttca ccacatccac aacccgttgt
     2401 ctgacacgaa ccgtcgcttg gtccagcggt aaagtacttg ccggagtctc cgtcaccgta
     2461 tcggccggta atgacgtcat aatttttgac atcattttcg gatcatcttt tgcaatctca
     2521 gaaggcagta caccatagat cgcaatcccc cacagaccaa acttaaacgg acctttcagg
     2581 gcagccagca catcggcaac ggctgacgac agcgcatcgc cggaaaagga taaagccagt
     2641 ccttcagccc ccggtgtagc cagggcaggc agaccgaagg ccatcgcggt ggccgaagac
     2701 tgtccaccac cagagctacc gctatgattg ccgctgttac caccattacc accgggttta
     2761 ctcccgccgt taccaaactc ccccggatta tagttatgaa tatctccatt cggcgtatgc
     2821 gtcgtacccc agcccggccc gctgtttgga tccgtaccac cacctgatga agggccacct
     2881 ttaccagacg taccgttcac attcccactg gcctgaccac cattatgtcc cagacctgaa
     2941 ttacccggac ctcgaccgtc tccaccactc atatcgtttt cctctttgac atttaaaaca
     3001 ataaattaaa accaaaatac tgtatatata cacagtatga ttattacctt actcctccag
     3061 ttttcaggtg tcaatatatg agattagttc tcatcttcag gcacaaaaaa gcgcccggag
     3121 ccgcctgtaa gcagatcgcg tcttttcccg aaaatttctc atggcaacaa attttattat
     3181 ttgagatatt catcacactt acttttatat tggtatagat gtacctgaaa gcgaaatggc
     3241 atgaggagag gacctgcggt taaactgttc gggaaacctg agacgcccgg aggccactat
     3301 gacgcacctg aaactgactc ccgctgaaga aaagcgcctg gcggacgcct ttgtcgctca
     3361 ggcactggcc atgggcctga aagggaacaa aacgctgcca aaggcggtaa aacaacaggt
     3421 gcgggaaagg gtgcttcgtg ccattcagga cgaacgtgaa gccaggcggg cgcaggaaat
     3481 gcgtcgggag aaatacgagg cggcggaaac caccttcacc tggcagccgc agcgccgccg
     3541 gtgaaaacga tttaaggttc tactttcagg tgtatccact tcggaagcat tttagggata
     3601 tggtattatc gaaaatcatg ctttcggcaa tccaatgggt ttatacgatt taaaaaggga
     3661 aaaacatgga caaaaggaca acgttagtag ctctggtttc tatcatcttt ttcacttctg
     3721 gatgtgatga ccagaaaaaa gggaatggca acgctgaatc gacacaaatc actaaaaacg
     3781 atataacaac tcaacttaag ttagataaaa cgcaacaacc tgacgatata gaaaagaaaa
     3841 taaaagaaat ccaggccaac tccacccctg aaaaaataaa aaaattcaat gaattaaaag
     3901 atgacgaccc taaaatccta cgagagtcgg ttgtttcaga aattaagaaa aaactaccac
     3961 tattggttga cgaagccaca ttaatgacag atgtatcaac tgacggaggt acattctcat
     4021 acaaatatgt aaccaaaggg atttcagcca gcacgatgga aagtgatgtg tggaaagatg
     4081 ccatgcaaaa aaacataaaa aatagctact gctctgacga tgccagacta aaagtattca
     4141 gagagctatt tcctgaaggt gtcatttaca actactactt aagtgacaaa ttaatctaca
     4201 catacaaggc acttccatcg atttgttcct aaagtattta ccataacgtc gcaagctggg
     4261 cgccccagcg ggcggctttt ctatatctgt cctataaatt cagaaaagga aagctaaatg
     4321 gctgcatata ccagcgaaac agcgaagttt ttacaaagcg tcagtagcga tcaaacatgg
     4381 tggaagtcac gatataatcc aggagacagc gttccccatt ctggcattta tcgatgtaca
     4441 gtctgcggaa aagagatcac cagtaacggc aacgacccgt tcccgccgca aaaccaccat
     4501 cagcacagtc agcaacaagc aatcaaatgg caactgattg tcagaacgga cactaaaggt
     4561 gaccgtttcg gaatcaaatg attgagagcc atccggagct ttttttgtac ctgaaactcg
     4621 aagatattta ccataacatc ccttatcggt accgccagta aggcactggc ggcgtttccg
     4681 gcggctacgg gataaaacaa gcaacaaccc acccggaaaa ccgccggaaa cgcgcccggt
     4741 aaattttatc ctaatgcatg cctatgcacg caactgcata gcatatgttt tgacgtaaaa
     4801 tggatgccgt aagccctttt tttaccgact ttctgcttca tcagcctgac tgacacgacc
     4861 atattctttc agaccgcccc ctgctaaact tcttcatatc accgcattaa cccgggaggc
     4921 tccgcaatga gcaacactac acactacgaa aacgccaact tcctgcggga actggctgaa
     4981 aacctgcccc gcatactgcc ggaaggtggc ccggacaaag ctgcgctgtt gcaacgcctg
     5041 gctaacgaag aactggccca ggcagagtat gaagatcagg ttcgcgctaa agtgaccgcc
     5101 gcccgcgctg acacgcgctc tggcatgacc acggaacaac ttcgccagag actgcatgga
     5161 cgctatcagg aactacgcga tgccgtatga ggtggaatgg aagcagggag ccatcgagga
     5221 tgtgacagca ttgtttgact acattgcgga aaattcatcc ctgtgggatg cgcagaacgt
     5281 taccgatcgc gttctcgctg cggcggacaa actggccgac ttcccgcgcc tctatgaagt
     5341 ggactcacgc tatggcgagg acgttcgccg gatcagcctg atgggacagc atatcctcta
     5401 tgatgttgat gaccaggccc ggaaagtccg ggttctggct gtggtcggcc agcgccagaa
     5461 tccccatacc gttagctaat aaccgatgac gggggcgcaa ttcattgcgt ggccgtcgcc
     5521 ggttggacaa gcgcagcgcg tcagcgtttt ccccagtgcc accaccggcg cgacgtttcg
     5581 ggatgtgtat ctgctgtctc cggtttgtgt tccagcagtc tcatggcctg catttcctgc
     5641 cgcagtccgg ctacctcttc ccgcagcgct ttaatctccg aaatcagtgc ctcagacacc
     5701 gatgcttcag tctcacccgg cagtctcaac ccgtcccgct cctgagtctc accctgtctt
     5761 aattcaccgt acacccggat aagctcgctg gtatccacca cccgaccacc atcagcttcc
     5821 gtacggtatg atacgcggcc tttcgccata tcacgataca gagaactccg ggctttcccc
     5881 gtcagttccg tcgcctgtct caccgtcacc cacgccattg tctcacctcc ggttttgaga
     5941 cagtataccc gacaggaagc acaccacacc cggaaggaca gaaaacatga cgaagcagga
     6001 aaaaactgcc ctcaatatgg ctaaattcat taaggaccag tcgctgctgt tgctcgagaa
     6061 actgaacgag ctggatatgg ataccccggc cgacatgtgc gagcggcttc acgaggatgc
     6121 ggaaacactc tggcaggtga tgcgggataa gctcgggaag gaataacagc accggccatg
     6181 ccggtgctgc cgtttatttt ttgccggacg gccaccaccg gcgggcgggt ttgtcctccg
     6241 gcagttcagg caccggcacg ttaaaatagc ggctgttata ttcccgcatc agctccgctt
     6301 tcagcttctc gttcaccgcg ctttccccgt gccgctcgct taccacctca atcatcgcgt
     6361 taatctgctc cggtgagcac ttcccggcat caaccacctg cacaccgttc tcgatggcga
     6421 accgctccac cttgcgatag aggtaatcac tcagcttcat tcctgtgggt ttgtcccgtt
     6481 cccattgcgg attttcaccg gtgaaaaagt acgtggcttt ttcgtcgctg ttgagccgct
     6541 cgtcatacat cccgatggat ttcatcatcg ccagatgctg ggcccggtcg acaaaacaga
     6601 aaacatacgc cccggtctgc caggcgagca ccaccatatc ttcaagatgc tcgctggtcg
     6661 gcatgatgca gcgctcgccg tcccggtcct ccgcgtcttc aataaatacc atgtacagcg
     6721 ccataatccc tcactccgtt gtcataaatt taaagcacgc aaacagccgt tcacgatcca
     6781 gctccgccgt tttgcgggcc tcgccggtct gtggattcac gatttcatcc accgacagga
     6841 tcgcccgctg caccctgacc tgcgtcgccc ggtccgggca gatgtaccag atttcatcga
     6901 tgccgttcgc ccggcggtta aacagatacc cggacaccac ctcctgatag cgcctgcggc
     6961 tcttcacggt gcgctccacc tcaaaagcca ccgttctgcc gtccagctcc gccagcgcgt
     7021 ccggcacctt catccctttc agggcaatac ggtgcaggta ccgccagccg gtggcccccc
     7081 gggcctccat ggcgaggcgc agggactgca cggccagccg gtgcggcagc tgcgccgccg
     7141 acacccgcga cggctgaaac ggaataatgt ccgtcagcgg ctcgtcctcg tcgaacgaga
     7201 acgccacccc gtgcggcgtc agcccccaca cgctgacctg acggccgtca accgggtatt
     7261 tcgcccggac cagcagccct ttcgccacca ggctgtccag gaagcggtaa accggctgta
     7321 ccgccgcaat ccccagccgt cgcgcaacca gctccgccgt ggaacacagc tcttcccgca
     7381 gccagcgcag ggtcagccgt gttttctccg cgttccgcgc ctgccgcttc gcgtggctga
     7441 cgttgtatcg ctccagtgcc atcagtacag ccccgcaaaa tcatcatcac ccggccgctg
     7501 ccgcctggcc tccggctggt actccgccag cgggtccgtt ttcgccagca ccgggaaggt
     7561 tttcagcgga atttcccggc gatcaacccg cagcgggctg ctgaacgcca gccgcgccac
     7621 gcccagcccg gtcatcaccg cgaacccgtc aggcagatgt tgcagggtgt tcacgtcaaa
     7681 cagcggccgc gcctccttct ggatgaagct gtccccgctg atatgctcca cgttccccgc
     7741 ctcccggctg gcccggcggc gctccacgtc cacccggatt tccccggtct ggcccgccgc
     7801 ccactgcgcg gactcctggc tggccgcccg gtagaaccag cggatcggcg tgttatccag
     7861 caccgtggtt ttcacaaaat cagcgggcag atcctggccg cactgcccga agtcgcccag
     7921 cgactggtgc gcgagcagca ggttgcagtc ccggctgcgc agcgtcccca gcgcgttcag
     7981 cacgtacttc gacagcaggt atttaatctc atcgagcatg atgctcgcgt gcggccacct
     8041 ccggaactca tcccgcgcaa tgatgatttg tgcgcagcgg gcaaacagca tcttctgcac
     8101 ccggatcacc gcctcatcgt ccatcgagcc gatgacgtac aggcagccgc cgccgttgat
     8161 tatccccgcc acgtcgatgc cgctgtccgt ctgcaggacg ctcaggtcgg ccacgttctc
     8221 cagcttggtg atcagcccct tcacgtcctt tttcagggcc tccggcagcg cgtacgccgc
     8281 cgccagcacc tgctgaatgt ttgccccctg cggaaactgc tccgcaatca gcttcgccgc
     8341 tttctgctcg ctgatgcggt agtggtccgc cacgtcgccg gtctcactca ggttaaaccc
     8401 ggcaaccagc agattcttca gggcgtactg gtcgagatcg cggaacagat tcagctgcgc
     8461 cggtttcccc ggccgcagat ccagcagggt gaagtttggg cagtgcgccc ggaagacgtg
     8521 cggcgcccac gcgtcccctt tggggtcgaa gtacaccacc gcgtccccga aatcgcgtac
     8581 gcagcgaatg agcgcgttgg tggccatcac gctcttgccg ctcccgggca ggccgaggat
     8641 ctgaatgttg gtcttgcgcc aggtatcacg cgggaccagc accggctggc cctgctcatc
     8701 cttacccaga aacaccgcct gctgtttccg ggcctgctcc gtggaaaaat cggccagcgc
     8761 gttgaccggt accggctccc cgaaatgcag gtggcggacg tcggtcattt caccggtccg
     8821 ctggctgctg cgccggctct cctggttcag tttcgcgttc accgcctcgt cagcagccgc
     8881 aaaatgaccg tcaagcagca ggaacgccag cagccgcgcc aacacaatcc cgacaaccag
     8941 cccgcccgcg caccaggcaa tcccgaacag gttagccggc tgcagcaggt aataatctgc
     9001 gttcgcgtag aggtccggac gcaggacggt ttctccccag ctctcgataa acacggcgac
     9061 aaccagcccc cccagaaggg ccagaccggc gctgccggtc agccagtacg cgtagttcca
     9121 gacatgccgc cgtgccctga ccatccgccc gggcgaatcc agcgaggcca gcaccgaaaa
     9181 tggcgcagtc cggttattat cgcgcttata gcgatcggca tactcccgtc gctccttatg
     9241 actcagcccc acaaaacgca tccgtccggc ccagagttca gccagaccga aaaacaggcc
     9301 gataaccagg aaagccccgg ccagaccgta atactccctg taaagccagc tgacccacgg
     9361 gtaaatctgc cgggccagct ccgggatatg ccactgccag gcaagcgaat ccgtattaaa
     9421 catgtaatct ctctccttca gacgacgggt ttaccggcgt ggtgtgcagc ccccgctgca
     9481 caagccgcgg acatgcccca agggatggcg gcggcgttca cgtccgtggt gactgcgttc
     9541 agctttcacc acggaggcca gcctctttcc gccccatgcg gaaaaggcgc agccccggag
     9601 gaacaacgag cacgccccag cgtgtgagag gaaaaggcgg caccccagtg cggtcttttc
     9661 cggtgttccg gcccccgtca gagttcgcca tagcgagcgg ctgaccaccc gcaaaatcag
     9721 aggcagcttt gctgcgtcgt caggtgcgtc cagcaccgtg gatttttgtg ggtggtcggg
     9781 cgtgagcctg acgggggccg ggtgaatttc tggcgtggcc ggcgtccagc cggacagagc
     9841 cagaaagagg gggaaagacc gtgcgtcggt ggcgtccagc cagcgtaagc aggggatttc
     9901 cccctgaagt cagccacgcc ccagcgggga tgacttgcat ttctccgcaa ggagaaaaaa
     9961 gcaacagaga taggttaacg taaaatgttc agcgaaaaac cgacatggtt cacctatcct
    10021 gataattgat cgtcagggaa tagaaagacg taatcagggg acaatatccc acatcagcgc
    10081 gtccggtgac cgtcttgcag gtgccgggga aattgcgcct cct
//
