LOCUS       AP022383               10123 bp    DNA     circular BCT 09-OCT-2020
DEFINITION  Citrobacter freundii STW0522-01 plasmid pSTW0522-01-3 DNA, complete
            sequence.
ACCESSION   AP022383
VERSION     AP022383.1
DBLINK      BioProject: PRJDB9036
            BioSample: SAMD00195843
            Sequence Read Archive: DRR199648
KEYWORDS    .
SOURCE      Citrobacter freundii
  ORGANISM  Citrobacter freundii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Citrobacter; Citrobacter freundii complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     complete genome sequences of Citrobacter freundii str. STW0522-01
            isolated from hospital sewage
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Sekizuka,T., Katagiri,M., Asai,K. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-FEB-2020) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v. 2.5; minimap v. 0.2-r124; racon v. 1.1.0;
            circlator v. 1.5.3; Pilon v. 1.18; SPAdes v. 3.11.1; A5-miseq v.
            20140604
            Genome Coverage       :: 356x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Citrobacter freundii"
                     /mol_type="genomic DNA"
                     /strain="STW0522-01"
                     /isolation_source="hospital sewage"
                     /db_xref="taxon:546"
                     /plasmid="pSTW0522-01-3"
                     /geo_loc_name="Japan:Tokyo"
                     /collection_date="2018-05-22"
     gene            complement(102..365)
                     /locus_tag="STW0522CIT01_P30010"
     CDS             complement(102..365)
                     /locus_tag="STW0522CIT01_P30010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33512.1"
                     /translation="MAAYTSETAKFLQSVSSDQTWWKSRYNPGDSVPHSGIYRCTVCGK
                     EITSNGNDPFPPQNHHQHSQQQAIKWQLIVRTDTKGDRFGIK"
                     /name="hypothetical protein"
     gene            complement(451..1017)
                     /locus_tag="STW0522CIT01_P30020"
     CDS             complement(451..1017)
                     /locus_tag="STW0522CIT01_P30020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33513.1"
                     /translation="MDKRTTLVALVSIIFFTSGCDDQKKGNGNAESTQITKNDITTQLK
                     LDKTQQPDDIEKKIKEIQANSTPEKIKKFNELKDDDPKILRESVVSEIKKKLPLLVDEA
                     TLMTDVSTDGGTFSYKYVTKGISASTMESDVWKDAMQKNIKNSYCSDDARLKVFRELFP
                     EGVIYNYYLSDKLIYTYKALPSICS"
                     /name="hypothetical protein"
     gene            complement(1139..1384)
                     /locus_tag="STW0522CIT01_P30030"
     CDS             complement(1139..1384)
                     /locus_tag="STW0522CIT01_P30030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33514.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEAAETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            1711..3396
                     /locus_tag="STW0522CIT01_P30040"
     CDS             1711..3396
                     /locus_tag="STW0522CIT01_P30040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33515.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSFSGDALSSAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADTVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPALQVSVPKGVPAAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKREYDKAKAELDA
                     EDKNITTLNGRITSTEKAIPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNAKLKAAQASLNAMNDALSRDKAALSGAMESRKQKEKKAKEAENKLNE
                     EKKKPRKGTKDYGHDYFPDPKTEDIKGLGELKEGKPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="hypothetical protein"
     gene            3406..3663
                     /locus_tag="STW0522CIT01_P30050"
     CDS             3406..3663
                     /locus_tag="STW0522CIT01_P30050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33516.1"
                     /translation="MGLKLHIHWFDKKTEEFKGGEYSKDFGDDGSVIESLGMPLKDNIN
                     NGWFDVEKPWVSILQPHFKNVIDISKFDYFVSFVYRDGNW"
                     /name="hypothetical protein"
     gene            3748..3897
                     /locus_tag="STW0522CIT01_P30060"
     CDS             3748..3897
                     /locus_tag="STW0522CIT01_P30060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33517.1"
                     /translation="MKKAKAIFLFILIVSGFLLVACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="hypothetical protein"
     gene            complement(4000..4581)
                     /locus_tag="STW0522CIT01_P30070"
     CDS             complement(4000..4581)
                     /locus_tag="STW0522CIT01_P30070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33518.1"
                     /translation="MRQNSRAAPVAFFHRLRPPARVHINRRFSGASVGAVRLNHESDST
                     GETRQDLKIPTVSGGSLPLALSCSDPAVYRIPVPPFSLTGSVALSHSSHTGISARCRSF
                     APSWAVSKNSPFSPTAAPYPVTVHLSPTRKSTVKRHWQQPLVTGSSQRICLAKHAVALE
                     VCAKVRLHWKDRFGCCALRKPVTTVKQFPN"
                     /name="hypothetical protein"
     gene            5383..7344
                     /locus_tag="STW0522CIT01_P30080"
     CDS             5383..7344
                     /locus_tag="STW0522CIT01_P30080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33519.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGSAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     VGIVLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPNFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QPEARRQRPGDDDFAGLY"
                     /name="hypothetical protein"
     gene            7344..8075
                     /locus_tag="STW0522CIT01_P30090"
     CDS             7344..8075
                     /locus_tag="STW0522CIT01_P30090"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33520.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="hypothetical protein"
     gene            8082..8612
                     /locus_tag="STW0522CIT01_P30100"
     CDS             8082..8612
                     /locus_tag="STW0522CIT01_P30100"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33521.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLSDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPSGKK"
                     /name="hypothetical protein"
     gene            complement(8640..8819)
                     /locus_tag="STW0522CIT01_P30110"
     CDS             complement(8640..8819)
                     /locus_tag="STW0522CIT01_P30110"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33522.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="hypothetical protein"
     gene            8888..9265
                     /locus_tag="STW0522CIT01_P30120"
     CDS             8888..9265
                     /locus_tag="STW0522CIT01_P30120"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33523.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQGETQERDGLRLPGETEASVSEALISEIKALREEVAGLRQEMQAMRLLEH
                     KPETADTHPETSRRWWHWGKR"
                     /name="hypothetical protein"
     gene            complement(9327..9626)
                     /locus_tag="STW0522CIT01_P30130"
     CDS             complement(9327..9626)
                     /locus_tag="STW0522CIT01_P30130"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33524.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHILYDVDDQARKVRVLAVVGQRQNPHTVS"
                     /name="hypothetical protein"
     gene            complement(9616..9879)
                     /locus_tag="STW0522CIT01_P30140"
     CDS             complement(9616..9879)
                     /locus_tag="STW0522CIT01_P30140"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBV33525.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRSGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     BGC             1..8396
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(4001..4581)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          7345..8072
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.473"
                     /coverage="100.0"
                     /evalue="7.29e-144"
ORIGIN
        1 gccggaaacg ccgccagtgc cttactggcg gtaccgataa gggatgttat ggtaaatatc
       61 ttcgagtttc aggtacaaaa aaagctccgg atggctctca atcatttgat tccgaaacgg
      121 tcacctttag tgtccgttct gacaatcagt tgccatttga ttgcttgttg ctgactgtgc
      181 tgatggtggt tttgcggcgg gaacgggtcg ttgccgttac tggtgatctc ttttccgcag
      241 actgtacatc gataaatgcc agaatgggga acgctgtctc ctggattata tcgtgacttc
      301 caccatgttt gatcgctact gacgctttgt aaaaacttcg ctgtttcgct ggtatatgca
      361 gccatttagc tttccttttc tgaatttata ggacagatat agaaaagccg cccgctgggg
      421 cgcccagctt gcgacgttat ggtaaatact ttaggaacaa atcgatggaa gtgccttgta
      481 tgtgtagatt aatttgtcac ttaagtagta gttgtaaatg acaccttcag gaaatagctc
      541 tctgaatact tttagtctgg catcgtcaga gcagtagcta ttttttatgt ttttttgcat
      601 ggcatctttc cacacatcac tttccatcgt gctggctgaa atccctttgg ttacatattt
      661 gtatgagaat gtacctccgt cagttgatac atctgtcatt aatgtggctt cgtcaaccaa
      721 tagtggtagt tttttcttaa tttctgaaac aaccgactct cgtaggattt tagggtcgtc
      781 atcttttaat tcattgaatt tttttatttt ttcaggggtg gagttggcct ggatttcttt
      841 tattttcttt tctatatcgt caggttgttg cgttttatct aacttaagtt gagttgttat
      901 atcgttttta gtgatttgtg tcgattcagc gttgccattc ccttttttct ggtcatcaca
      961 tccagaagtg aaaaagatga tagaaaccag agctactaac gttgtccttt tgtccatgtt
     1021 tttccctttt taaatcgtat aaacccattg gattgccgaa agcatgattt tcgataatac
     1081 catatcccta aaatgcttcc gaagtggata cacctgaaag tagaacctta aatcgttttc
     1141 accggcggcg ctgcggctgc caggtgaagg tggtttccgc cgcctcgtat ttctcccgac
     1201 gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc accctttccc
     1261 gcacctgttg ttttaccgcc tttggcagcg ttttgttccc tttcaggccc atggccagtg
     1321 cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt ttcaggtgcg
     1381 tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg tcctctcctc
     1441 atgccatttc gctttcaggt acatctatac caatataaaa gtaagtgtga tgaatatctc
     1501 aaataataaa atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct gcttacaggc
     1561 ggctccgggc gcttttttgt gcctgaagat gagaactaat ctcatatatt gacacctgaa
     1621 aactggagga gtaaggtaat aatcatactg tgtatatata cagtattttg gttttaattt
     1681 attgttttaa atgtcaaaga ggaaaacgat atgagtggtg gagacggtcg aggtccgggt
     1741 aattcaggtc tgggacataa tggtggtcag gccagtggga atgtgaacgg tacgtctggt
     1801 aaaggtggcc cttcatcagg tggtggtacg gatccaaaca gcgggccggg ctggggtacg
     1861 acgcatacgc cgaatggaga tattcataac tataatccgg gggagtttgg taacggcggg
     1921 agtaaacccg gtggtaatgg tggtaacagc ggcaatcata gcggtagctc tggtggtgga
     1981 cagtcttcgg ccaccgcgat ggccttcggt ctgcctgccc tggctacacc gggggctgaa
     2041 ggactggctt tatccttttc cggcgatgcg ctgtcgtcag ccgttgccga tgtgctggct
     2101 gccctgaaag gtccgtttaa gtttggtctg tgggggattg cgatctatgg tgtactgcct
     2161 tctgagattg caaaagatga tccgaaaatg atgtcaaaaa ttatgacgtc attaccggcc
     2221 gatacggtga cggagactcc ggcaagtact ttaccgctgg accaagcgac ggttcgtgtc
     2281 agacaacggg ttgtggatgt ggtgaaggat gagcggcagc atattgcggt tgtcgcgggt
     2341 cggccaatga gtgttcctgt ggtggatgcg aaaccgacaa aacgtccggg ggtattcagt
     2401 gtgtctattc cgggtctccc ggctctgcag gtgagcgtac cgaaaggtgt tccggcagcg
     2461 aaagccccgc caaaaggcat tgttgctgaa aaaggtgatt cacgtccggc tggttttacg
     2521 gccggtggta actcccgtga ggccgttatt cgtttcccga aagagaccgg acagaagccg
     2581 gtctatgtgt cggtgacaga tgttcttacc ccggcacagg taaaacagcg tcaggaggaa
     2641 gaaaagcgtc gccagcaggc atgggacgcc gctcatccgg aagaggggct gaaaagagaa
     2701 tatgataaag cgaaagctga gctggatgcc gaagataaaa atattacgac tttaaacggc
     2761 aggattacat cgacagaaaa agcgattcct ggcgccaggg ctgctgtcca ggaagctgat
     2821 aaaaaggtga aagaggcgga ggcgaataag gatgattttg tgacttataa ccctcctcat
     2881 gaatatggct ccgggtggca ggatcaggtt cgctatcttg ataaggatat tcagaatcag
     2941 aatgcgaaat taaaagctgc tcaggcatct ttaaacgcaa tgaatgatgc cttatccaga
     3001 gataaggctg cgctttccgg ggcgatggag agccggaaac aaaaggagaa aaaagcgaag
     3061 gaggcagaaa ataaattaaa tgaggaaaag aaaaagcctc gcaagggaac taaagattat
     3121 ggccatgatt attttcctga tcccaagact gaagatatta aggggttggg agagttgaaa
     3181 gagggaaaac ctaaaacccc taaacaagga ggaggtggta agcgggcccg atggtatggt
     3241 gataaaaagc gtaaaattta tgaatgggat tcccagcacg gtgagcttga aggataccgc
     3301 gccagtgatg gcgaacacct cggcgcattc gatccaaaaa cgggtaagca ggttaaaggg
     3361 ccggatccaa aacgaaacat taaaaaatat ctttaagagg taaatatggg gcttaaatta
     3421 catattcatt ggtttgataa gaaaaccgaa gagtttaaag gcggtgaata ctcaaaagac
     3481 ttcggtgatg atggttctgt cattgaaagt ctggggatgc ctttaaagga taatattaat
     3541 aatggttggt ttgatgttga aaaaccatgg gtttcgatat tacagccaca ctttaaaaat
     3601 gtaatcgata ttagtaaatt tgattacttt gtatcctttg tttaccggga tggtaactgg
     3661 taagcaaaag ttatcaagga tgagttgaaa tacaggcttc cggtgttcac ggatgaatgc
     3721 cgaagcctct gaatacaagg aaggtatatg aaaaaggcaa aggctatttt tctttttata
     3781 ttgatagttt caggctttct tctggtcgca tgtcaggcaa actatatccg ggatgttcag
     3841 ggtggaacgg tggcaccatc gtcctcttct gaactgacgg ggatcgcggt tcagtagaaa
     3901 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatcttttgc cctgtaaacg
     3961 aaaaaaccac ctggggaggt ggtttgatcg aaggttaagt cagttgggga actgcttaac
     4021 cgtggtaact ggctttcgca gagcacagca accaaatctg tccttccagt gtagccggac
     4081 tttggcgcac acttcaagag caaccgcgtg tttagctaaa caaatcctct gcgaactccc
     4141 agttaccaat ggctgctgcc agtggcgttt taccgtgctt ttccgggttg gactcaagtg
     4201 aacagttacc ggataaggcg cagcagtcgg gctgaacggg gagttcttgc ttacagccca
     4261 gcttggagcg aacgacctac accgagccga gataccagtg tgtgagctat gagaaagcgc
     4321 cacacttccc gtaagggaga aaggcggaac aggtatccgg taaacggcag ggtcggaaca
     4381 ggagagcgca agagggagcg acccgccgga aacggtgggg atctttaagt cctgtcgggt
     4441 ttcgcccgta ctgtcagatt catggttgag cctcacggct cccacagatg caccggaaaa
     4501 gcgtctgttt atgtgaactc tggcaggagg gcggagccta tggaaaaacg ccaccggcgc
     4561 ggccctgctg ttttgcctca catgttagtc ccctgcttat ccacggaatc tgtgggtaac
     4621 tttgtatgtg tccgcagcgc ccgccgcagt ctcacgcccg gagcgtagcg accgagtgag
     4681 cgaggaggcg caatttcccc ggcacctgca agacggtcac cggacgcgct gatgtgggat
     4741 attgtcccct gattacgtct ttctattccc tgacgatcaa ttatcaggat aggtgaacca
     4801 tgtcggtttt tcgctgaaca ttttacgtta acctatctct gttgcttttt tctccttgcg
     4861 gagaaatgca agtcatcccc gctggggcgt ggctgacttc agggggaaat cccctgctta
     4921 cgctggctgg acgccaccga cgcacggtct ttccccctct ttctggctct gtccggctgg
     4981 acgccggcca cgccagaaat tcacccggcc cccgtcaggc tcacgcccga ccacccacaa
     5041 aaatccacgg tgctggacgc acctgacgac gcagcaaagc tgcctctgat tttgcgggtg
     5101 gtcagccgct cgctatggcg aactctgacg ggggccggaa caccggaaaa gaccgcactg
     5161 gggtgccgcc ttttcctctc acacgctggg gcgtgctcgt tgttcctccg gggctgcgcc
     5221 ttttccgcat ggggcggaaa gaggctggcc tccgtggtga aagctgaacg cagtcaccac
     5281 ggacgtgaac gccgccgcca tcccttgggg catgtccgcg gcttgtgcag cgggggctgc
     5341 acaccacgcc ggtaaacccg tcgtctgaag gagagagatt acatgtttaa tacggattcg
     5401 cttgcctggc agtggcatat cccggagctg gcccggcaga tttacccgtg ggtcagctgg
     5461 ctttacaggg agtattacgg tctggccggg gctttcctgg ttatcggcct gtttttcggt
     5521 ctggctgaac tctgggccgg acggatgcgt tttgtggggc tgagtcataa ggagcgacgg
     5581 gagtatgccg atcgctataa gcgcgataat aaccggactg cgccattttc ggtgctggcc
     5641 tcgctggatt cgcccgggcg gatggtcagg gcacggcggc atgtctggaa ctacgcgtac
     5701 tggctgaccg gcagcgccgg tctggccctt ctgggggggc tggttgtcgc cgtgtttatc
     5761 gagagctggg gagaaaccgt cctgcgtccg gacctctacg cgaacgcaga ttattacctg
     5821 ctgcagccgg ctaacctgtt cgggattgcc tggtgcgcgg gcgggctggt tgtcgggatt
     5881 gtgttggcgc ggctgctggc gttcctgctg cttgacggtc attttgcggc tgctgacgag
     5941 gcggtgaacg cgaaactgaa ccaggagagc cggcgcagca gccagcggac cggtgaaatg
     6001 accgacgtcc gccacctgca tttcggggag ccggtaccgg tcaacgcgct ggccgatttt
     6061 tccacggagc aggcccggaa acagcaggcg gtgtttctgg gtaaggatga gcagggccag
     6121 ccggtgctgg tcccgcgtga tacctggcgc aagaccaaca ttcagatcct cggcctgccc
     6181 gggagcggca agagcgtgat ggccaccaac gcgctcattc gctgcgtacg cgatttcggg
     6241 gacgcggtgg tgtacttcga ccccaaaggg gacgcgtggg cgccgcacgt cttccgggcg
     6301 cactgcccaa acttcaccct gctggatctg cggccgggga aaccggcgca gctgaatctg
     6361 ttccgcgatc tcgaccagta cgccctgaag aatctgctgg ttgccgggtt taacctgagt
     6421 gagaccggcg acgtggcgga ccactaccgc atcagcgagc agaaagcggc gaagctgatt
     6481 gcggagcagt ttccgcaggg ggcaaacatt cagcaggtgc tggcggcggc gtacgcgctg
     6541 ccggaggccc tgaaaaagga cgtgaagggg ctgatcacca agctggagaa cgtggccgac
     6601 ctgagcgtcc tgcagacgga cagcggcatc gacgtggcgg ggataatcaa cggcggcggc
     6661 tgcctgtacg tcatcggctc gatggacgat gaggcggtga tccgggtgca gaagatgctg
     6721 tttgcccgct gcgcacaaat catcattgcg cgggatgagt tccggaggtg gccgcacgcg
     6781 agcatcatgc tcgatgagat taaatacctg ctgtcgaagt acgtgctgaa cgcgctgggg
     6841 acgctgcgca gccgggactg caacctgctg ctcgcgcacc agtcgctggg cgacttcggg
     6901 cagtgcggcc aggatctgcc cgctgatttt gtgaaaacca cggtgctgga taacacgccg
     6961 atccgctggt tctaccgggc ggccagccag gagtccgcgc agtgggcggc gggccagacc
     7021 ggggaaatcc gggtggacgt ggagcgccgc cgggccagcc gggaggcggg gaacgtggag
     7081 catatcagcg gggacagctt catccagaag gaggcgcggc cgctgtttga cgtgaacacc
     7141 ctgcaacatc tgcctgacgg gttcgcggtg atgaccgggc tgggcgtggc gcggctggcg
     7201 ttcagcagcc cgctgcgggt tgatcgccgg gaaattccgc tgaaaacctt cccggtgctg
     7261 gcgaaaacgg acccgctggc ggagtaccag ccggaggcca ggcggcagcg gccgggtgat
     7321 gatgattttg cggggctgta ctgatggcac tggagcgata caacgtcagc cacgcgaagc
     7381 ggcaggcgcg gaacgcggag aaaacacggc tgaccctgcg ctggctgcgg gaagagctgt
     7441 gttccacggc ggagctggtt gcgcgacggc tggggattgc ggcggtacag ccggtttacc
     7501 gcttcctgga cagcctggtg gcgaaagggc tgctggtccg ggcgaaatac ccggttgacg
     7561 gccgtcaggt cagcgtgtgg gggctgacgc cgcacggggt ggcgttctcg ttcgacgagg
     7621 acgagccgct gacggacatt attccgtttc agccgtcgcg ggtgtcggcg gcgcagctgc
     7681 cgcaccggct ggccgtgcag tccctgcgcc tcgccatgga ggcccggggg gccaccggct
     7741 ggcggtacct gcaccgtatt gccctgaaag ggatgaaggt gccggacgcg ctggcggagc
     7801 tggacggcag aacggtggct tttgaggtgg agcgcaccgt gaagagccgc aggcgctatc
     7861 aggaggtggt gtccgggtat ctgtttaacc gccgggcgaa cggcatcgat gaaatctggt
     7921 acatctgccc ggaccgggcg acgcaggtca gggtgcagcg ggcgatcctg tcggtggatg
     7981 aaatcgtgaa tccacagacc ggcgaggccc gcaaaacggc ggagctggat cgtgaacggc
     8041 tgtttgcgtg ctttaaattt atgacaacgg agtgagggat tatggcgctg tacatggtat
     8101 ttattgaaga cgcggaggac cgggacggcg agcgctgcat catgccgacc agcgagcatc
     8161 ttgaagatat ggtggtgctc gcctggcaga ccggggcgta tgttttctgt tttgtcgacc
     8221 gggcccagca tctggcgatg atgaaatcca tcgggatgta tgacgagcgg ctcaacagcg
     8281 acgaaaaagc cacgtacttt ttcaccggtg aaaatccgca atgggaacgg gacaaaccca
     8341 caggaatgaa gctgagtgat tacctctatc gcaaggtgga gcggttcgcc atcgagaacg
     8401 gtgtgcaggt ggttgatgcc gggaagtgct caccggagca gattaacgcg atgattgagg
     8461 tggtaagcga gcggcacggg gaaagcgcgg tgaacgagaa gctgaaagcg gagctgatgc
     8521 gggaatataa cagccgctat tttaacgtgc cggtgcctga actgccggag gacaaacccg
     8581 cccgccggtg gtggccgtcc ggcaaaaaat aaacggcagc accggcatgg ccggtgctgt
     8641 tattccttcc cgagcttatc ccgcatcacc tgccagagtg tttccgcatc ctcgtgaagc
     8701 cgctcgcaca tgtcggccgg ggtatccata tccagctcgt tcagtttctc gagcaacagc
     8761 agcgactggt ccttaatgaa tttagccata ttgagggcag ttttttcctg cttcgtcatg
     8821 ttttctgtcc ttccgggtgt ggtgtgcttc ctgtcgggta tactgtctca aaaccggagg
     8881 tgagacaatg gcgtgggtga cggtgagaca ggcgacggaa ctgacgggga aagcccggag
     8941 ttctctgtat cgtgatatgg cgaaaggccg cgtatcatac cgtacggaag ctgatggtgg
     9001 tcgggtggtg gataccagcg agcttatccg ggtgtacggt gaattaagac agggtgagac
     9061 tcaggagcgg gacgggttga gactgccggg tgagactgaa gcatcggtgt ctgaggcact
     9121 gatttcggag attaaagcgc tgcgggaaga ggtagccgga ctgcggcagg aaatgcaggc
     9181 catgagactg ctggaacaca aaccggagac agcagataca catcccgaaa cgtcgcgccg
     9241 gtggtggcac tggggaaaac gctgacgcgc tgcgcttgtc caaccggcga cggccacgca
     9301 atgaattgcg cccccgtcat cggttattag ctaacggtat ggggattctg gcgctggccg
     9361 accacagcca gaacccggac tttccgggcc tggtcatcaa catcatagag gatatgctgt
     9421 cccatcaggc tgatccggcg aacgtcctcg ccatagcgtg agtccacttc atagaggcgc
     9481 gggaagtcgg ccagtttgtc cgccgcagcg agaacgcgat cggtaacgtt ctgcgcatcc
     9541 cacagggatg aattttccgc aatgtagtca aacaatgctg tcacatcctc gatggctccc
     9601 tgcttccatt ccacctcata cggcatcgcg tagttcctga tagcgtccat gcagtctctg
     9661 gcgaagttgt tccgtggtca tgccagagcg cgtgtcagcg cgggcggcgg tcactttagc
     9721 gcgaacctga tcttcatact ctgcctgggc cagttcttcg ttagccaggc gttgcaacag
     9781 cgcagctttg tccgggccac cttccggcag tatgcggggc aggttttcag ccagttcccg
     9841 caggaagttg gcgttttcgt agtgtgtagt gttgctcatt gcggagcctc ccgggttaat
     9901 gcggtgatat gaagaagttt agcagggggc ggtctgaaag aatatggtcg tgtcagtcag
     9961 gctgatgaag cagaaagtcg gtaaaaaaag ggcttacggc atccatttta cgtcaaaaca
    10021 tatgctatgc agttgcgtgc ataggcatgc attaggataa aatttaccgg gcgcgtttcc
    10081 ggcggttttc cgggtgggtt gttgcttgtt ttatcccgta gcc
//
