LOCUS       AP022280                4450 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W19-CRE-02_6 DNA, complete genome,
            strain: WP8-W19-CRE-02.
ACCESSION   AP022280
VERSION     AP022280.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194638
            Sequence Read Archive: DRR199474
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W19-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 360x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W19-CRE-02"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W19-CRE-02_6"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2019-02-05"
     gene            101..316
                     /locus_tag="WP8W19C02_P60010"
     CDS             101..316
                     /locus_tag="WP8W19C02_P60010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93303.1"
                     /translation="MITKQQKTALNMAKFIQAQSLLLLEKLNELDLDAEADMCEKLHED
                     AEMLCRTLSVQLNALQDAVNGKNIDQ"
                     /name="hypothetical protein"
     gene            294..680
                     /locus_tag="WP8W19C02_P60020"
     CDS             294..680
                     /locus_tag="WP8W19C02_P60020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93304.1"
                     /translation="MAKISISEAARLTGKSRTTLHRLIKTGELSTCHGARNSRLVDVSE
                     LIRVFGPLEYSPVEHVTAQASEQSDTGISPQSEQVIAQLRQEVEHLKTLVSAKDSHIDS
                     LKQAMLLIEHKHIGDSPWWKFWKR"
                     /name="hypothetical protein"
     gene            1664..1900
                     /locus_tag="WP8W19C02_P60030"
     CDS             1664..1900
                     /locus_tag="WP8W19C02_P60030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93305.1"
                     /translation="MGHPVGKYISTDEEHELNYWLKKNGFKESESNRKSLVTLIYAAKS
                     GLDMDSGDHLEHVELDEYFEQSKSAWLDDFEKK"
                     /name="hypothetical protein"
     gene            3079..3570
                     /locus_tag="WP8W19C02_P60040"
     CDS             3079..3570
                     /locus_tag="WP8W19C02_P60040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93306.1"
                     /translation="MTSVFQSSDLSKGVMSARTTKNAFVINPNTGGLSFGKIPANNFGI
                     AEGEIYLKLGLIGFGKSREGAFGARHVWEKHKKDLAIPSPEDTAQVVADILQEGVEVIV
                     NFNAKKQDIRPVVLNTTKGRVVLEEKVIDGVACYTIISAYGNKNAPGTVIGNLEKPTED
                     "
                     /name="hypothetical protein"
     Typing          1184..1261
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.585"
                     /coverage="88.0"
                     /evalue="1.98e-14"
     Typing          complement(3820..4319)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.389"
                     /coverage="99.0"
                     /evalue="4.76e-153"
ORIGIN
        1 agaatgctcc gtccgctcgc cgcagacaag tgaccgagcg tagcgagcga acggtcgagg
       61 aagcggaaaa taaccaaata tgacttggag cactgacgct gtgattacaa agcaacagaa
      121 aacggcattg aacatggcga aattcattca ggcgcaatcc ctgcttttgc tggagaagct
      181 caacgaactg gatctcgatg ctgaggctga catgtgtgaa aaacttcatg aggatgcaga
      241 gatgctctgc aggacactct cggtgcagtt aaacgctcta caggatgcag ttaatggcaa
      301 aaatatcgat cagtgaggcc gcacggctga cagggaagag ccggacaaca ttgcacaggt
      361 tgattaagac aggtgagctg tccacctgtc atggcgcaag gaattctaga ttggttgatg
      421 tttcggagct tattcgtgtt tttggtcctc ttgagtatag cccggttgaa catgtgactg
      481 cgcaggcatc cgaacagtct gatacaggta tttccccgca gagtgaacag gtcattgcac
      541 agctgaggca ggaagtggaa cacctgaaaa cgttagtttc cgccaaggat tcgcacatcg
      601 atagcctcaa acaagcgatg ctgctgatcg agcacaaaca tatcggcgat tcaccctggt
      661 ggaagttctg gaaacgctga gaacgcctca gttgccggcg tggcttgtct gatttactga
      721 atgacttaat ccaaacctgt ttttccggcc ggcccagaca ccgcgatcgc cgctctctgc
      781 cttcggggtc aaaagatcac accccacgca agactatgtc ttgcaggttt tatcttttat
      841 atcagtaata taaccttaaa tatttcaggt gatttacatt taccccgaaa agtatcacac
      901 atttagcata cctccaggtg tcggtaccgg cactgtcata tcgccggatt gtggctatcg
      961 ctatttttca gtggcttaca tgtcacacaa tccggcactg gtgtcggatc cacacacctg
     1021 tatacccgcg agcagtgccg gatccgtcca catgatattt atccaggtgg ccggattgtg
     1081 tgactgatat atatccagtg tggtgctgga tccggcactg tggcttgacg gcatttttag
     1141 ggtattttca cggattcaat ttttttgagg gtgtcagcgc tacgggacag gatgtgcatt
     1201 tgtaataccg cgcacacaca gcactccaaa tgctcctgtc agggctcgcc gccccgacac
     1261 cgggcaagcg cttcaaggca acgaaatgaa ttacgttgcc ttgaagcgcc gtcttgtttg
     1321 tttgccttta gttagataat tattctgtat ttgtttttct tctaacttac tgctttgaaa
     1381 ggaaaacttt attatgatat ctatctaaca tcgatatgat gttcgttgga agataagaaa
     1441 atgccaggaa aaaagtgccc cttctgtaat aacctcacgc tctttggaaa tagctgcaca
     1501 tcttgtaatg ctgtggttcg aacaccgatt aatgaaggtc gtggcggaag aggtaaacaa
     1561 tgtcctttct gtaagacaat gactttgttt aaagatgggg gtaatggcgc actgaagtgt
     1621 tcaatgtgcc agcgtaaatt cacacagcca taaggataaa atcatgggac accctgttgg
     1681 gaagtacata tccaccgatg aggaacacga actaaattat tggctaaaaa agaacggatt
     1741 caaggaaagt gaaagcaaca ggaaatcact agtgacactg atttatgctg ctaaatcagg
     1801 tctagatatg gatagtggtg atcatcttga acatgttgag ttagatgaat actttgagca
     1861 atcaaaatcg gcatggcttg acgactttga aaagaaataa aaaaaacaga caaagcccgc
     1921 tatgagtggg ctttgttcta tatgttaaaa ctatttggca cagatgctca tctgtcactg
     1981 gcgaaaagtg tgaaaaacag gcgaatctga ggtcttcggg gtctaaatcc cgcgccatgc
     2041 agacgggtgc atagctattc accatctcag gattcatctg cgacgtttta cgggcttttt
     2101 gcatagtaag ctggggtttt catcgtcttc accctcaaaa cgctctgagc gcgtttctgc
     2161 ggtgcgcgta atgacgcgtt atggtaaatc gtgttgtcca gctttctgcc aggcctgcct
     2221 acatcgtagg tttcccgttt ggaaaaaggc ctgaaaattt cacccacccc ctcccttaca
     2281 acccacccca tggataactt ggataacaga gagacgcgga taacgcgata actcggataa
     2341 catagttttt ttaaaaaata attttaatac aataagttgc cgaattttat tgcaattaat
     2401 attgcgtttc acttttttaa gtgacagttt tctttgcaat ttaatgcttg agttgttgcg
     2461 tttgaagtta ttgaattaca cctgttattt caactaaatc gttgaaaata aaggtttttt
     2521 tgttattatt agtgtgccga gaagaaggag ggtgtatgtc taagaaatca gagatggttg
     2581 ggcgaaaagt tcaagaagaa aaaaggttgg cgcttcgtca acaagtatgg ggaagcgaac
     2641 tcgagcaatt agatttgtgg gataaagcaa cccataaagg atttgcatca gtgccaagaa
     2701 cactacctca actatcccgt attatggata tgtttgcagg aaaaggtact ccggtatcaa
     2761 caacttacct tgctcttttg tgtaatgtat ttgatagcag ttttgtcgaa ataaaggaaa
     2821 agaaacgatt agcttttgag tcaggatttt cggggcaaag agcgataaca gcatggcata
     2881 gtcgcatgag aaagcttgag tccatggggt tcataagtgc aaaggctggt atgcatgatg
     2941 attttagtta tgtgcttatt cttaatccat tttctgctgt caatcgcctt tatgagggaa
     3001 gggataagga tgaattttat tatggtttga ttagccgaat gtcagacgtt ggaacagtta
     3061 tgtaatggag gagaaagaat gacatcagtc tttcagtctt cagacctcag taaaggcgtg
     3121 atgtctgcaa gaacgacgaa aaatgcattt gttataaacc caaacactgg cggtttatcg
     3181 tttggaaaga ttccagctaa caattttgga atcgctgagg gcgagatcta cttaaaactg
     3241 ggattgattg gttttggtaa gtccagagaa ggggcttttg gggctaggca tgtgtgggaa
     3301 aagcacaaaa aggatttagc tatcccaagt cctgaagata cagctcaggt tgtagctgat
     3361 atcttgcaag aaggggttga ggttattgtt aattttaatg ctaaaaaaca agatattaga
     3421 cccgttgtat tgaacacaac taaagggcga gtggttttag aggagaaagt tattgatggt
     3481 gttgcctgtt acacgattat ttctgcatat gggaacaaga acgcaccagg aacggtaatt
     3541 ggaaacttag agaaacctac cgaagactga agtgcttcgg taggaaaatt agaatgatag
     3601 ctcgtggctt ctctttagct ctttggggtc agagaacatg ctcaatgagc atgtggctat
     3661 caacaagcta tcccagctaa gcttggtgac tacgcattag aaaacttgat ctgggtcgtt
     3721 ttctaaagaa gccctttcac atagatgatc atgcttcacg ggtcttctgt tgtcaattat
     3781 tttacaaaat gccgactact tgatgcgatt aactagcgga tcttcttgag atcctgacct
     3841 ctccatcgta gtctttgact tcgtaaacga aaaaaccacc cggcaaggtg gttttttcga
     3901 aggttcagta accggcaagt ttctgaaccg cggtaactgg ctattagagg agcacagtca
     3961 ccaaaacttg ttctttcagt ttagccttaa ctggcgcata acttcaagac taactcctct
     4021 aaatcagtta ccagtggctg ctgccagtgg cgcgttgtcg tgtctttccg ggttggactc
     4081 aagtcgatag ttaccggata aggcgcagca gtcggactga acggggggtt cgtgcataca
     4141 gtccagcttg gagcgaactg cctacccgga accgagtgtc aggcgtggaa tgagataaac
     4201 gcggccataa cagcggaatg gcaccggtaa accgaaaggc aggaacagga gagcgcacga
     4261 gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact
     4321 gatcttagta tctgattctg agatgcttgt caggggggcg aagcctatgg aaaaacgccc
     4381 agtatggcgt ggccttctcg cttccttctg gtgttttctt tacgtctact gccaacctta
     4441 tttattcctt
//
