LOCUS       AP022279                4898 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W19-CRE-02_5 DNA, complete genome,
            strain: WP8-W19-CRE-02.
ACCESSION   AP022279
VERSION     AP022279.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194638
            Sequence Read Archive: DRR199474
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W19-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 360x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W19-CRE-02"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W19-CRE-02_5"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2019-02-05"
     gene            101..439
                     /locus_tag="WP8W19C02_P50010"
     CDS             101..439
                     /locus_tag="WP8W19C02_P50010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93293.1"
                     /translation="MPTEQPKRLKTIKVRVTETEWASLRAACPKAQLAEWMREHCLGVV
                     PQQRRTPPPTIDPALLRQVAGIGNNLNQIARRINASPLTTLDKVEYLAALAAVARELEG
                     LRRDRQNP"
                     /name="hypothetical protein"
     gene            420..983
                     /locus_tag="WP8W19C02_P50020"
     CDS             420..983
                     /locus_tag="WP8W19C02_P50020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93294.1"
                     /translation="MIVKIHSRGVGRGSGPVEYLLGKDGNRSGATLDRGDPEMVRELID
                     GSRYAQRYTSGVLSFHEPDLPRETKDRLMDEFEQALLPGLDADQYAVLWVEHRDKDRLE
                     LNFVVPERRTVVRQTPSAVFPRGGRPPDQRMANGHQWRLGIARPRRPNQSAGRDYGQRP
                     TPEQAGSRTSHYRRLAGHGCGGAG"
                     /name="hypothetical protein"
     gene            1109..1543
                     /locus_tag="WP8W19C02_P50030"
     CDS             1109..1543
                     /locus_tag="WP8W19C02_P50030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93295.1"
                     /translation="MSKILDMAKTFEQTSKQQADDIESSLKPEFERHERAIKEALDSSR
                     QSINDAIQDQQKLIGWLLLKSWGVMLISGLLLLAATSGTLWYQGNLIAERQATLETLGK
                     KGGKLIFNKCEDENGKIRLCIRMNMDEGKFGDDYMIPKGY"
                     /name="hypothetical protein"
     gene            1547..1780
                     /locus_tag="WP8W19C02_P50040"
     CDS             1547..1780
                     /locus_tag="WP8W19C02_P50040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93296.1"
                     /translation="MTELERHLLNALETLQSEQDRQLSGLQTACESLLRQFETTKKENA
                     SLLGEVKVLRQQCEHLNEQVSSLATQVASLKA"
                     /name="hypothetical protein"
     gene            1777..1992
                     /locus_tag="WP8W19C02_P50050"
     CDS             1777..1992
                     /locus_tag="WP8W19C02_P50050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93297.1"
                     /translation="MIAQQTAQTQEGLLDEYQQAHAERDLYFNRKHEQKEISGLPIDKA
                     REYLQEGLEGRIERQQVRERGRGFEM"
                     /name="hypothetical protein"
     gene            2116..2355
                     /locus_tag="WP8W19C02_P50060"
     CDS             2116..2355
                     /locus_tag="WP8W19C02_P50060"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93298.1"
                     /translation="MVAAAMRSKRTVSVTLEPSLLERARAAGINLSATLAAALQHEISG
                     SEAERWKRENSAAIQELNRITEEHGLLSDDYRAF"
                     /name="hypothetical protein"
     gene            2355..2663
                     /locus_tag="WP8W19C02_P50070"
     CDS             2355..2663
                     /locus_tag="WP8W19C02_P50070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001160966.1"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein CcdB"
                     /protein_id="BBT93299.1"
                     /translation="MQFTIYRNKGNARAYPYLLDVQSDIIGELATRIVIPLFPLAAFTG
                     RPAQRLNPVIIVEGDKYLVMTQDMAAVRQTQLGEEVTGVQESRQLIKDAIDFLMDGV"
                     /name="plasmid maintenance protein CcdB"
     gene            2778..3209
                     /locus_tag="WP8W19C02_P50080"
     CDS             2778..3209
                     /locus_tag="WP8W19C02_P50080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_015943189.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93300.1"
                     /translation="MNIPTHLKHKPVLEVINYDQIDGPYANDSDAMGLSIGIAQWNSPG
                     WTDLSAKVWRHTGEKWSRQSEELPLHRVIDLTTLICIAMEYCRNGRLSSSDDFPVSMST
                     DNADLGHHVELMKKELKSGSKYLDESLKRLSLELKRLGY"
                     /name="hypothetical protein"
     gene            3371..4357
                     /locus_tag="WP8W19C02_P50090"
     CDS             3371..4357
                     /locus_tag="WP8W19C02_P50090"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93301.1"
                     /translation="MTEKAFLRNLPHRPYCTDDKSSGLLIRPQATALGYRHIQYNPPPH
                     VASLVFDLDKPDSYHAWQDAGLPAPHWISVNRTNGHAHIGYMLATPVARTSAARQKPLR
                     YLAAIEHVLAKRLGADMGYAGLITKNPTHSDWLTIWHQIEPYSLDYLAEFCPDADLAAY
                     SRRSRKEATGLGRNVTVFDNVREWAYTAIRAHWRPNGYDAWLCAVQAACECTNVFGLEQ
                     GGPLPHSEIKATAKSIARWTWRNLTPSTFADYVDRTHTPEIQAVRGAKGGRIGGKVSKG
                     GGRPSKAASLLPVVLQLKAQGYSNRDIAEDLQISAGSVSNYLRRDRE"
                     /name="hypothetical protein"
     gene            complement(4510..4800)
                     /locus_tag="WP8W19C02_P50100"
     CDS             complement(4510..4800)
                     /locus_tag="WP8W19C02_P50100"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93302.1"
                     /translation="MKDNADKGTGDMFGIKRGRGRPKTGSAKTGAERQAAYRTKQQENN
                     VTVTINRGLLEGLDAQMQAIRDGGSAVVLTSEQAGEILRALRTAEKKQLRG"
                     /name="hypothetical protein"
     Typing          962..1989
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="89.244"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 taagcaagga cacacagggg gcccgcttcg cggtctatcc cctgtggcct tgttcagggg
       61 gcaagccccc cgaaaccccc gaaaaaaacg gaggccatcg atgccgacag agcagccaaa
      121 acggctcaaa acgatcaagg tcagagtcac tgaaaccgag tgggccagct tgcgggcggc
      181 ctgccccaaa gcccagttgg cggagtggat gcgcgagcac tgcttggggg ttgttcccca
      241 gcagcggcgc accccaccgc caaccatcga cccggccttg ctccggcagg tggccgggat
      301 cgggaacaac ctcaaccaga tcgcccggcg catcaatgcc agcccgctga ccacgttgga
      361 caaggtggaa taccttgcgg cgttagccgc cgtggcccga gaactggagg ggctacgccg
      421 tgatcgtcaa aatccatagc cggggagtcg gtcggggatc tggcccggtg gaatacctgc
      481 ttggcaaaga cgggaaccgg agcggggcca cgctggatcg cggcgacccc gagatggtca
      541 gggagttgat agacggcagc cgttacgccc agcggtacac cagcggcgtg ctgtcgttcc
      601 atgaaccgga cttgccgagg gagaccaagg atcggttgat ggacgagttc gagcaagccc
      661 tgttgcccgg actggacgcc gatcagtatg cggtgctgtg ggtcgagcac cgagacaaag
      721 accgcctaga gctgaatttc gtggtgcccg aacgtcgaac tgttgtccgg caaacgcctt
      781 cagccgtatt tccacggggc ggacggccac cggatcaacg catggcgaac ggccatcaat
      841 ggcgacttgg gattgcacga cccagacgac ccaatcaatc ggcaggccgt gactacggcc
      901 aaagacctac cccggaacaa gctggaagcc gaacaagcca ttacaggcgg cttgctgggc
      961 atggctgcgg cggggctggt taaaaaccgg gaggacgtgc tcaagaccct gaccggggcg
     1021 gggttcgacg ttaccaggac aaccaagaac agtatcagca ttgccgaccc tgaagggggc
     1081 cggaatatcc gcctaaaggg ggcgatctat gagcaagatt ttagacatgg caaagacctt
     1141 cgagcagaca tcgaagcagc aagccgacga tatagagagc agtctgaagc cagagttcga
     1201 gaggcacgag cgagctatca aagaagcgtt ggactcaagc aggcagagca tcaacgacgc
     1261 catccaagac cagcagaagc tgatcggctg gctcctgctc aagagctggg gagtgatgtt
     1321 gatctcgggc ttgctgttgc tggccgcgac gagcgggacg ctgtggtatc aggggaacct
     1381 gatcgccgag cggcaggcca ccttggagac gttgggcaaa aagggcggga aattgatctt
     1441 caacaagtgc gaggacgaga acgggaagat ccggctttgc atcaggatga acatggacga
     1501 agggaagttc ggggacgatt acatgatacc gaagggctac taaacgatga ccgagctaga
     1561 acggcactta ttgaacgcgc tagagacctt gcagagcgag caagatcggc aattgagcgg
     1621 cttgcaaaca gcgtgcgaga gcttgctacg gcagttcgag acgacgaaaa aagaaaacgc
     1681 gagcttgctg ggagaagtga aagtcttgcg ccagcagtgc gagcacttga acgagcaggt
     1741 cagcagcttg gcaacgcagg tcgccagctt gaaggcatga tcgcgcagca gacggcgcag
     1801 acgcaggagg ggttattgga cgaataccag caggcccacg cagaacggga tctctatttc
     1861 aatcggaagc acgagcagaa agagatctcc ggcttgccga tagacaaggc cagagagtac
     1921 ctacaggaag gactggaggg gcggatagag cggcagcagg tccgcgaacg ggggcgtggc
     1981 tttgagatgt agctgccgcg ctgcgcttgg cggccccagc cccgagcgcg acagacttcg
     2041 ctagtcgcac ccgtcgcaag ggcgtgatat tatcatacac gtgctatcgc acgtgttaat
     2101 ttattggagg tcatcatggt cgcagcagca atgcgcagca agagaacggt cagcgtcacg
     2161 ctggagccca gcttgctgga acgagccaga gcggcgggga tcaacctgtc cgccaccctt
     2221 gcagccgcac tacagcatga aatcagcgga tcagaagcag aacgctggaa gcgtgagaat
     2281 agtgcagcca tacaggagct taaccgcatc accgaggagc acggtttgct ttccgacgat
     2341 tacagggcgt tctgatgcag ttcacgatct accgcaacaa gggcaatgcc cgcgcttacc
     2401 cctatctgct ggatgtgcaa agcgacatca tcggcgagct ggccaccaga atagtgatcc
     2461 cactgttccc gctggcggcc ttcaccgggc ggccagcgca acggctgaat ccggtaatta
     2521 tcgttgaggg ggataagtat ctggtgatga cgcaggacat ggcagcggtg aggcagacgc
     2581 agcttgggga agaagtcaca ggagtgcagg agtctcgtca gctcatcaag gacgcaatag
     2641 atttcctgat ggatggtgtt tgaggcttta aagggaggca gatatttcgg cggttccggc
     2701 gtacaacccc atagacatta ccaactaaac tcagtaatgt ttgtgaaatt agtaaacttg
     2761 ataatctggg gctcgctatg aacattccaa ctcatttgaa gcacaagccg gtactggaag
     2821 ttattaacta tgaccagatt gatggtccat atgctaatga ctctgatgca atgggactgt
     2881 caattggcat cgcacaatgg aactcacctg gatggactga cctctcagcc aaagtatggc
     2941 gtcatacagg cgagaaatgg tcaagacagt cagaagaact accactgcat cgtgttatcg
     3001 acttaacgac attgatatgt atcgcgatgg aatactgtcg caatggtcgc ttgtcatcta
     3061 gcgacgattt cccggttagc atgtctactg ataacgctga tttggggcat catgttgagc
     3121 taatgaaaaa agagctcaag agcgggagta aatatctcga tgaaagtcta aaacgactgt
     3181 ctttagagct aaaaaggctg ggttactaac ggggctatgc ggtaacttat tgctcataaa
     3241 aagacaaacc cccgttatcg ctggaatctt ggcggatctg cgaaaaacgg gggctgtgac
     3301 gaatcacaag agaagtctac cacatggatc acaccaaaca accctgcacc gccaataagc
     3361 gcggtgatcg atgacagaaa aagcctttct caggaatctc ccccatcggc cctattgcac
     3421 tgacgataaa tcgtcagggc tgctcattcg cccgcaggct accgcactgg gctatcgcca
     3481 tatccagtac aacccaccgc cgcatgtggc gtctttggtg ttcgacttgg ataaaccgga
     3541 cagttaccac gcttggcagg atgcaggctt gccagccccg cactggatca gcgttaaccg
     3601 cacgaacggc cacgcccata ttgggtacat gctggcgacc ccagtagccc gtaccagcgc
     3661 cgcccggcaa aagccgctgc gatacctagc cgccatagaa cacgttctag cgaagcggct
     3721 gggggccgac atggggtatg cggggctgat caccaaaaac cccacccata gcgattggct
     3781 caccatctgg caccaaatcg agccctacag cctcgactat ctcgcagagt tctgcccgga
     3841 tgccgatttg gcggcctaca gccgccgcag tcgcaaagag gcgactgggc taggccgtaa
     3901 cgtcactgtg ttcgacaacg tgcgggaatg ggcctacacg gcgattagag cgcattggcg
     3961 gcctaatggg tacgatgcat ggctctgtgc cgtgcaagct gcctgtgagt gcaccaacgt
     4021 gtttggcttg gagcagggtg gcccgttgcc ccatagcgag atcaaggcca cggccaagag
     4081 catcgcccgt tggacgtggc ggaatctaac cccgtccacg ttcgcggatt atgttgatcg
     4141 gacgcataca ccagagattc aggccgtcag aggggctaag ggcgggcgta tcggtggcaa
     4201 ggtatccaag ggcggaggca ggccgagcaa agctgcttct ctcctacctg tggtgctaca
     4261 actcaaggcg cagggttaca gcaatcgcga tattgcggag gatctgcaaa tttcggctgg
     4321 cagtgtgtcc aactacctcc gccgtgatcg tgagtgatca aaaacaaagc ctatatcaga
     4381 taacagcgcc cgtagggcgt tccttgagcc ctaaaagcat tcaagagaga ggatcttggc
     4441 gagggggggt aacggcgatc ccgtagggct gtccatcagg ccatgtgttc ggcctgtggg
     4501 cagtagctgt tatccccgga gctgtttttt ctctgctgtt cgtagcgccc gcaagatctc
     4561 cccggcctgt tccgaggtca gcaccaccgc agaacccccg tctctgatcg cctgcatctg
     4621 ggcatccagt ccttccaata gccctctatt tatcgtcaca gtaacgttat tctcctgctg
     4681 ttttgtccgg taggccgctt gtcgttctgc ccctgttttg gctgatccgg ttttgggtct
     4741 accccgtccg cgcttgatgc caaacatgtc tccggttccc ttatcagcgt tgtctttcat
     4801 cgtcctaatt cccgtcacag tgaccataaa tattatctgt cactgtgaca caaaaaccat
     4861 taatccgcac cctcaaaatc caacccatgt taaagtac
//
