LOCUS       AP022278               13354 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W19-CRE-02_4 DNA, complete genome,
            strain: WP8-W19-CRE-02.
ACCESSION   AP022278
VERSION     AP022278.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194638
            Sequence Read Archive: DRR199474
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W19-CRE-02 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13354)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 360x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..13354
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W19-CRE-02"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W19-CRE-02_4"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2019-02-05"
     gene            101..1594
                     /locus_tag="WP8W19C02_P40010"
     CDS             101..1594
                     /locus_tag="WP8W19C02_P40010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001120888.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="BBT93280.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91 family transposase"
     gene            1625..2152
                     /locus_tag="WP8W19C02_P40020"
     CDS             1625..2152
                     /locus_tag="WP8W19C02_P40020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93281.1"
                     /translation="MLRSQRRGRDPPCWLSEKEPLVGKRRVNFQRCWLPVSRIGLHRRG
                     VERVNCGPKLLDLGEKGVYSSYTWATPLLRREYKEEEMNYQIVNIAECSNYQLEAANIL
                     TEAFNDLGNNSWPDMTSATKEVKECIESPNLCFGLLINNSLVGWIGLRPMYKETWELHP
                     LVVRPDYQNCHF"
                     /name="hypothetical protein"
     gene            complement(2253..4382)
                     /locus_tag="WP8W19C02_P40030"
     CDS             complement(2253..4382)
                     /locus_tag="WP8W19C02_P40030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93282.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANTQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="hypothetical protein"
     gene            4581..4865
                     /locus_tag="WP8W19C02_P40040"
     CDS             4581..4865
                     /locus_tag="WP8W19C02_P40040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93283.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="hypothetical protein"
     gene            5419..6912
                     /locus_tag="WP8W19C02_P40050"
     CDS             5419..6912
                     /locus_tag="WP8W19C02_P40050"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_001120888.1"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="BBT93284.1"
                     /translation="MPHVAARTASRDRDTGRYQSHRPEQTLLYQIVDEYYPAFAALMAE
                     QGKELPGYVQREFEEFLQCGRLEHGFLRVRCESCHAEHLVAFSCKRRGFCPSCGARRMA
                     ESAALLVDEVLPEQPMRQWVLSFPFQLRFLFASRPEIMGWVLGIVYRVIATHLVKKAGH
                     THQVAKTGAVTLIQRFGSALNLNVHFHMLFLDGVYVEQSHGSARFRWVKAPTSPELTQL
                     THTIAHRVGRYLERQGLLERDVENSYLASDAVDDDPMTPLLGHSITYRIAVGSQAGRKV
                     FTLQTLPTSGDPFGDGIGKVAGSSLHAGVAARADERKKLERLCRYISRPAVSEKRLSLT
                     RGGNVRYQLKTPYRDGTTHVIFEPLDFIARLAALVPKPRVNLTRFHGVFAPNSRHRALV
                     TPAKRGRGNKVRVADEPATPAQRRASMTWAQRLKRVFNIDIETCSGCGGAMKVIACIED
                     PIVIKQILDHLKHKAETSGTRALPESRAPPAELLLGLFD"
                     /name="IS91 family transposase"
     ARG             1626..2152
                     /name="AAC(6')-Ia"
                     /gene="AAC(6')-Ia"
                     /gene_name="AAC(6')"
                     /tool="rgi"
                     /drug_class="aminoglycoside antibiotic"
                     /identity="100.0"
                     /coverage="94.59"
     ARG             7190..7743
                     /name="aac(6')-Ia"
                     /gene="aac(6')-Ia"
                     /gene_name="aminoglycoside 6'-N-acetyltransferase
                     AAC(6')-Ia"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            7837..8001
                     /locus_tag="WP8W19C02_P40070"
     CDS             7837..8001
                     /locus_tag="WP8W19C02_P40070"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93286.1"
                     /translation="MIAGGYEFELPPIPCTIRLRDVLERMTDAELALQPGEIADQERER
                     CRRKPCSTS"
                     /name="hypothetical protein"
     gene            complement(8101..8952)
                     /locus_tag="WP8W19C02_P40080"
     CDS             complement(8101..8952)
                     /locus_tag="WP8W19C02_P40080"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000240536.1"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="BBT93287.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="replication protein"
     gene            complement(8939..9778)
                     /locus_tag="WP8W19C02_P40090"
     CDS             complement(8939..9778)
                     /locus_tag="WP8W19C02_P40090"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /inference="similar to AA sequence:RefSeq:WP_000880559.1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93288.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="hypothetical protein"
     gene            complement(9808..10014)
                     /locus_tag="WP8W19C02_P40100"
     CDS             complement(9808..10014)
                     /locus_tag="WP8W19C02_P40100"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93289.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="hypothetical protein"
     gene            complement(10016..10228)
                     /locus_tag="WP8W19C02_P40110"
     CDS             complement(10016..10228)
                     /locus_tag="WP8W19C02_P40110"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93290.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            complement(10289..12418)
                     /locus_tag="WP8W19C02_P40120"
     CDS             complement(10289..12418)
                     /locus_tag="WP8W19C02_P40120"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93291.1"
                     /translation="MAIYHLTAKTGSRSGGQSARAKADYIQREGKYARDMDEVLHAESG
                     HMPEFVERPADYWDAADLYERANGRLFKEVEFALPVELTLDQQKALASEFAQHLTGAER
                     LPYTLAIHAGGGENPHCHLMISERINDGIERPAAQWFKRYNGKTPEKGGAQKTEALKPK
                     AWLEQTREAWADHANRALERAGHDARIDHRTLEAQGIERLPGVHLGPNVVEMEGRGIRT
                     DRADVALNIDTANTQIIDLQEYREAIDHERNRQSEEIQRHQRVSGADRTAGPEHGDTGR
                     RSPAGHEPDPAGQRGAGGGVAESPAPDRGGMGGAGQRVAGGSRRGEQRRAERPERVAGV
                     ALEAMANRDAGFHDAYGGAADRIVALARPDATDNRGRLDLAALGGPMKNDRTLQAIGRQ
                     LKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHG
                     LVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEY
                     DADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQI
                     EQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQK
                     LASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPA
                     PRQRGMDRGGPDFSM"
                     /name="hypothetical protein"
     gene            12617..12901
                     /locus_tag="WP8W19C02_P40130"
     CDS             12617..12901
                     /locus_tag="WP8W19C02_P40130"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT93292.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="hypothetical protein"
     Typing          complement(4472..4627)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(12508..12663)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          complement(8940..9761)
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(2257..4382)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.859"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(10293..12418)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="99.859"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 tggcaatgtc tagcaacggc aggcatttcg gctgagggta aaagaacttt ccgctaagcg
       61 atagactgta tgtaaacaca gtattgcaag gacgcggaac atgcctcatg tggcggccag
      121 gacggccagc cgggatcggg atactggtcg ttaccagagc caccgacccg agcaaaccct
      181 tctctatcag atcgttgacg agtattaccc ggcattcgct gcgcttatgg cagagcaggg
      241 aaaggaattg ccgggctatg tgcaacggga atttgaagaa tttctccaat gcgggcggct
      301 ggagcatggc tttctacggg ttcgctgcga gtcttgccac gccgagcacc tggtcgcttt
      361 cagctgtaag cgtcgcggtt tctgcccgag ctgtggggcg cggcggatgg ccgaaagtgc
      421 cgccttgctg gttgatgaag tactgcctga acaacccatg cgtcagtggg tgttgagctt
      481 cccgtttcag ctgcgtttcc tgtttgccag ccggcccgag atcatggggt gggtgctggg
      541 catcgtttac cgcgtcattg ccacgcacct ggtcaagaaa gcgggccata cccaccaagt
      601 ggccaagacg ggcgcggtca ccctgatcca gcgttttgga tcggcgctca atctgaatgt
      661 tcacttccac atgctgtttc tcgacggtgt gtatgtcgag caatcccacg gctcagcgcg
      721 tttccgctgg gtcaaggcgc cgaccagccc agagctcacc cagctgacgc acaccatcgc
      781 ccaccgggtg ggtcgctatc tggaacggca aggcctgctg gaacgggatg tcgaaaacag
      841 ctatctggcc tcggatgcgg tggatgacga cccgatgaca cccctgctgg ggcactcgat
      901 cacttaccgt atcgctgtcg gttcacaggc ggggcgaaag gtgttcactt tgcaaactct
      961 gccgaccagt ggtgatccgt tcggtgacgg gattggcaag gtagccgggt ccagcctgca
     1021 cgccggcgtg gcggccaggg ccgatgaacg caagaagctc gaacggctgt gccggtacat
     1081 cagccgcccg gcggtatccg agaagcggct gtcgttaaca cgaggcggca acgtgcgcta
     1141 ccagctcaag acgccgtacc gggacggcac cacgcacgtc attttcgaac cattggattt
     1201 cattgcaagg ctggccgccc tggtaccgaa gcccagagtc aacctaaccc gcttccacgg
     1261 ggtgttcgca cccaacagtc ggcaccgggc gttggtcacg ccggcaaaac ggggcagggg
     1321 caacaaggtc agggtggctg atgaaccggc aacaccagca caacggcgag cgtcgatgac
     1381 atgggcgcaa cggctcaagc gtgttttcaa tatcgacatc gagacctgca gcggctgcgg
     1441 cggcgccatg aaagtcatcg cctgcattga agaccctata gtgatcaagc agatccttga
     1501 tcacctgaag cacaaagccg aaaccagcgg gaccagggcg ttacccgaaa gccgggcgcc
     1561 accggctgag ctgctcctgg gtctgtttga ctgacgagcc tgaaggccaa cgataccaat
     1621 caaaatgctg cgttcacagc gccgcggcag ggatccgccg tgctggttgt cggaaaagga
     1681 gccgctagtg ggaaagagga gggtaaattt tcagcgttgc tggctccccg tcagccggat
     1741 tgggttgcat cgcaggggtg tcgaaagagt caactgcggt ccaaagctgt tggacttggg
     1801 tgaaaagggc gtttattctt cctatacgtg ggcgacgccg ctattgcggc gcgaatacaa
     1861 agaggaagag atgaattatc aaattgtgaa tattgcggaa tgcagcaatt atcagttaga
     1921 agcagcaaat atactaacag aagcgttcaa tgatcttggt aacaattcat ggccagatat
     1981 gacgagtgca acaaaagaag taaaagaatg tattgagagt ccaaaccttt gtttcggtct
     2041 gctaataaat aactccttag ttggctggat aggcttaagg ccaatgtaca aggaaacctg
     2101 ggaattgcat ccattggttg tcagaccaga ttatcaaaat tgtcactttt gatcaggtag
     2161 accgaccctg aagcgctttt ttcgtattcc ataaaacccc cttctgtgcg tgagtactca
     2221 tagtataaca ggcgtgagta ccaacgcaag cactacatgc tgaaatctgg cccgcccctg
     2281 tccatgcctc gctggcgggg tgccggtgcc cgtgccagct cggcccgcgc aagctggacg
     2341 ctgggcagac ccatgacctt gctgacggtg cgctcgatgt aatccgcttc gtggccgggc
     2401 ttgcgctctg ccagcgctgg gctggcctcg gccatggcct tgccgatttc ctcggcactg
     2461 cggccccggc tggccagctt ctgcgcggcg ataaagtcgc acttgctgag gtcatcaccg
     2521 aagcgcttga ccagcccggc catctcgctg cggtactcgt ccagcgccgt gcgccggtgg
     2581 cggctaagct gccgctcggg cagttcgagg ctggccagcc tgcgggcctt ctcctgctgc
     2641 cgctgggcct gctcgatctg ctggccagcc tgctgcacca gcgccgggcc agcggtggcg
     2701 gtcttgccct tggattcacg cagcagcacc cacggctgat aaccggcgcg ggtggtgtgc
     2761 ttgtccttgc ggttggtgaa gcccgccaag cggccatagt ggcggctgtc ggcgctggcc
     2821 gggtcggcgt cgtactcgct ggccagcgtc cgggcaatct gcccccgaag ttcaccgcct
     2881 gcggcgtcgg ccaccttgac ccatgcctga tagttcttcg ggctggtttc cactaccagg
     2941 gcaggctccc ggccctcggc tttcatgtca tccaggtcaa actcgctgag gtcgtccacc
     3001 agcaccagac catgccgctc ctgctcggcg ggcctgatat acacgtcatt gccctgggca
     3061 ttcatccgct tgagccatgg cgtgttctgg agcacttcgg cggctgacca ttcccggttc
     3121 atcatctggc cggtggtggc gtccctgacg ccgatatcga agcgctcaca gcccatggcc
     3181 ttgagctgtc ggcctatggc ctgcaaagtc ctgtcgttct tcatcgggcc accaagcgca
     3241 gccagatcga gccgtcctcg gttgtcagtg gcgtcaggtc gagcaagagc aacgatgcga
     3301 tcagcagcac caccgtaggc atcatggaag ccagcatcac ggttagccat agcttccagt
     3361 gccacccccg cgacgcgctc cgggcgctct gcgcggcgct gctcacctcg gcggctacct
     3421 cccgcaactc tttggccagc tccacccatg ccgcccctgt ctggcgctgg gctttcagcc
     3481 actccgccgc ctgcgcctcg ctggcctgct gggtctggct catgacctgc cgggcttcgt
     3541 cggccagtgt cgccatgctc tgggccagcg gttcgatctg ctccgctaac tcgttgatgc
     3601 ctctggattt cttcactctg tcgattgcgt tcatggtcta ttgcctcccg gtattcctgt
     3661 aagtcgatga tctgggtgtt ggcggtgtcg atgttcaggg ccacgtctgc ccggtcggtg
     3721 cggatgcccc ggccttccat ctccaccacg ttcggcccca ggtgaacacc gggcaggcgc
     3781 tcgatgccct gcgcctcaag tgttctgtgg tcaatgcggg cgtcgtggcc agcccgctct
     3841 aatgcccggt tggcatggtc ggcccatgcc tcgcgggtct gctcaagcca tgccttgggc
     3901 ttgagcgctt cggtcttctg tgccccgccc ttctccgggg tcttgccgtt gtaccgcttg
     3961 aaccactgag cggcgggccg ctcgatgccg tcattgatcc gctcggagat catcaggtgg
     4021 cagtgcgggt tctcgccgcc accggcatgg atggccagcg tatacggcag gcgctcggca
     4081 ccggtcaggt gctgggcgaa ctcggacgcc agcgccttct gctggtcgag ggtcagctcg
     4141 accggcaggg caaattcgac ctccttgaac agccgcccat tggcgcgttc atacaggtcg
     4201 gcagcatccc agtagtcggc gggccgctcg acgaactccg gcatgtgccc ggattcggcg
     4261 tgcaagactt catccatgtc gcgggcatac ttgccttcgc gctggatgta gtcggccttg
     4321 gccctggccg attggccgcc cgacctgctg ccggttttcg ccgtaaggtg ataaatcgcc
     4381 atgctgcctc gctgttgctt ttgcttttcg gctccatgca atggccctcg gagagcgcac
     4441 cgcccgaagg gtggccgtta ggccagtttc tcgaagagaa accggtaagt gcgccctccc
     4501 ctacaaagta gggtcgggat tgccgccgct gtgcctccat gatagcctac gagacagcac
     4561 attaacaatg gggtgtcaag atggttaagg ggagcaacaa ggcggcggat cggctggcca
     4621 agctcgaaga acaacgagcg cgaatcaatg ccgaaattca gcgggtgcgg gcaagggaac
     4681 agcagcaaga gcgcaagaac gaaacaaggc gcaaggtgct ggtgggggcc atgattttgg
     4741 ccaaggtgaa cagcagcgag tggccggagg atcggctcat ggcggcaatg gatgcgtacc
     4801 ttgaacgcga ccacgaccgc gccttgttcg gtctgccgcc acgccagaag gatgagccgg
     4861 gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt
     4921 agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg
     4981 tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta
     5041 gcctagcgca gcgaatagac cagctatccg gcctctggcc gggcatattg ggcaagggca
     5101 gcagcgcccc acaagggcgc tgataaccgc gcctagtgga ttattcttag ataatcatgg
     5161 atggattttt ccaacacccc gccagccccc gcccctgctg ggtttgcagg tttgggggcg
     5221 tgacagttat tgcaggggtt cgtgacagtt attgcagggg ggcgtgacag ttattgcagg
     5281 ggttcgtgac agttagtacg ggagtgacgg gcactggctg gcaatgtcta gcaacggcag
     5341 gcatttcggc tgagggtaaa agaactttcc gctaagcgat agactgtatg taaacacagt
     5401 attgcaagga cgcggaacat gcctcatgtg gcggccagga cggccagccg ggatcgggat
     5461 actggtcgtt accagagcca ccgacccgag caaacccttc tctatcagat cgttgacgag
     5521 tattacccgg cattcgctgc gcttatggca gagcagggaa aggaattgcc gggctatgtg
     5581 caacgggaat ttgaagaatt tctccaatgc gggcggctgg agcatggctt tctacgggtt
     5641 cgctgcgagt cttgccacgc cgagcacctg gtcgctttca gctgtaagcg tcgcggtttc
     5701 tgcccgagct gtggggcgcg gcggatggcc gaaagtgccg ccttgctggt tgatgaagta
     5761 ctgcctgaac aacccatgcg tcagtgggtg ttgagcttcc cgtttcagct gcgtttcctg
     5821 tttgccagcc ggcccgagat catggggtgg gtgctgggca tcgtttaccg cgtcattgcc
     5881 acgcacctgg tcaagaaagc gggccatacc caccaagtgg ccaagacggg cgcggtcacc
     5941 ctgatccagc gttttggatc ggcgctcaat ctgaatgttc acttccacat gctgtttctc
     6001 gacggtgtgt atgtcgagca atcccacggc tcagcgcgtt tccgctgggt caaggcgccg
     6061 accagcccag agctcaccca gctgacgcac accatcgccc accgggtggg tcgctatctg
     6121 gaacggcaag gcctgctgga acgggatgtc gaaaacagct atctggcctc ggatgcggtg
     6181 gatgacgacc cgatgacacc cctgctgggg cactcgatca cttaccgtat cgctgtcggt
     6241 tcacaggcgg ggcgaaaggt gttcactttg caaactctgc cgaccagtgg tgatccgttc
     6301 ggtgacggga ttggcaaggt agccgggtcc agcctgcacg ccggcgtggc ggccagggcc
     6361 gatgaacgca agaagctcga acggctgtgc cggtacatca gccgcccggc ggtatccgag
     6421 aagcggctgt cgttaacacg aggcggcaac gtgcgctacc agctcaagac gccgtaccgg
     6481 gacggcacca cgcacgtcat tttcgaacca ttggatttca ttgcaaggct ggccgccctg
     6541 gtaccgaagc ccagagtcaa cctaacccgc ttccacgggg tgttcgcacc caacagtcgg
     6601 caccgggcgt tggtcacgcc ggcaaaacgg ggcaggggca acaaggtcag ggtggctgat
     6661 gaaccggcaa caccagcaca acggcgagcg tcgatgacat gggcgcaacg gctcaagcgt
     6721 gttttcaata tcgacatcga gacctgcagc ggctgcggcg gcgccatgaa agtcatcgcc
     6781 tgcattgaag accctatagt gatcaagcag atccttgatc acctgaagca caaagccgaa
     6841 accagcggga ccagggcgtt acccgaaagc cgggcgccac cggctgagct gctcctgggt
     6901 ctgtttgact gacgagcctg aaggccaacg ataccaatca aaatgctgcg ttcacagcgc
     6961 cgcggcaggg atccgccgtg ctggttgtcg gaaaaggagc cgctagtggg aaagaggagg
     7021 gtaaattttc agcgttgctg gctccccgtc agccggattg ggttgcatcg caggggtgtc
     7081 gaaagagtca actgcggtcc aaagctgttg gacttgggtg aaaagggcgt ttattcttcc
     7141 tatacgtggg cgacgccgct attgcggcgc gaatacaaag aggaagagat gaattatcaa
     7201 attgtgaata ttgcggaatg cagcaattat cagttagaag cagcaaatat actaacagaa
     7261 gcgttcaatg atcttggtaa caattcatgg ccagatatga cgagtgcaac aaaagaagta
     7321 aaagaatgta ttgagagtcc aaacctttgt ttcggtctgc taataaataa ctccttagtt
     7381 ggctggatag gcttaaggcc aatgtacaag gaaacctggg aattgcatcc attggttgtc
     7441 agaccagatt atcaaaataa aggtattggc aagatcctgc ttaaggaatt agaaaacaga
     7501 gctagagagc aaggtattat tggaatcgct ttaggaacag atgatgaata ctatagaaca
     7561 agtctctctt taataactat aacagaagat aatatatttg attcaataaa aaatattaaa
     7621 aatattaata aacatccata tgagttttat cagaagaatg gttattatat tgttggaata
     7681 attccaaatg ccaatggtaa aaacaaacca gatatttgga tgtggaaaag tttaatcaaa
     7741 gagtaaaaca atggaacaga ttaataacat agaacttgta gatccatcta tctacccaac
     7801 agatgaaatc ttaaaaagaa tacgattgcg gccgaaatga tcgccggcgg ctatgagttc
     7861 gagctgccgc ccattccttg caccatccgc ctacgcgacg tgctggagcg catgaccgat
     7921 gctgagctag cattgcagcc gggcgagatc gccgaccagg agcgtgaacg ctgccggcgc
     7981 aagccgtgtt caacctcatg atctggtcat ggtatttttc atggcactga gcctgatagt
     8041 tcttgcaaat tgttgtcact aaagggtttt gtgtgcttgt ttacaatcga gtgggggggg
     8101 tcagcctgcc gccttgggcc gggtgatgtc gtacttgccc gccgcgaact cggttaccgt
     8161 ccagcccagc gcgaccagct ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat
     8221 ggtcgaacca ctggcctctg acggccagac atagccgcac aaggtatcta tggaagcctt
     8281 gccggttttg ccggggtcga tccagccaca cagccgctgg tgcagcaggc gggcggtttc
     8341 gctgtccagc gcccgcacct cgtccatgct gatgcgcaca tgctggccgc cacccatgac
     8401 ggcctgcgcg atcaaggggt tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc
     8461 gtactccgac agcagccgaa acccctgccg cttgcggcca ttctgggcga tgatggatac
     8521 cttccaaagg cgctcgatgc agtcctgtat gtgcttgagc gccccaccac tatcgacctc
     8581 tgccccgatt tcctttgcca gcgcccgata gctacctttg accaccatgg catcagcggt
     8641 gacggcctcc cacttgggtt ccaggaacag ccggagctgc cgtccgcctt cggtcttggg
     8701 ttccgggcca agcactaggc cattaggccc agccatggcc accagccctt gcaggatgcg
     8761 cagatcatca gcgcccagcg gctccgggcc gctgaactcg atccgcttgc cgtcgccgta
     8821 gtcatacgtc acgtccagct tgctgcgctt gcgctcgccc cgcttgaggg cacggaacag
     8881 gccgggggcc agacagtgcg ccgggtcgtg ccggacgtgg ctgaggctgt gcttgttctt
     8941 aggcttcacc acggggcacc cccttgctct tgcgctgcct ctccagcacg gcgggcttga
     9001 gcaccccgcc gtcatgccgc ctgaaccacc gatcagcgaa cggtgcgcca tagttggcct
     9061 tgctcacacc gaagcggacg aagaaccggc gctggtcgtc gtccacaccc cattcctcgg
     9121 cctcggcgct ggtcatgctc gacaggtagg actgccagcg gatgttatcg accagtaccg
     9181 agctgccccg gctggcctgc tgctggtcgc ctgcgcccat catggccgcg cccttgctgg
     9241 catggtgcag gaacacgata gagcacccgg tatcggcggc gatggcctcc atgcgaccga
     9301 tgacctgggc catggggccg ctggcgtttt cttcctcgat gtggaaccgg cgcagcgtgt
     9361 ccagcaccat caggcggcgg ccctcggcgg cgcgcttgag gccgtcgaac cactccgggg
     9421 ccatgatgtt gggcaggctg ccgatcagcg gctggatcag caggccgtca gccacggctt
     9481 gccgttcctc ggcgctgagg tgcgccccaa gggcgtgcag gcggtgatga atggcggtgg
     9541 gcgggtcttc ggcgggcagg tagatcaccg ggccggtggg cagttcgccc acctccagca
     9601 gatccggccc gcctgcaatc tgtgcggcca gttgcagggc cagcatggat ttaccggcac
     9661 caccgggcga caccagcgcc ccgaccgtac cggccaccat gttgggcaaa acgtagtcca
     9721 gcggtggcgg cgctgctgcg aacgcctcca gaatattgat aggcttatgg gtagccattg
     9781 attgcctcct ttgcaggcag ttggtggtta ggcgctggcg gggtcactac ccccgccctg
     9841 cgccgctctg agctcttcca ggcactcgcg cagcgcctcg tattcgtcgt cggtcagcca
     9901 gaacttgcgc tgacgcatcc ctttggcctt catgcgctcg gcatatcgcg cttggcgtac
     9961 agcgtcaggg ctggccagca ggtcgccggt ctgcttgtcc ttttggtctt tcatatcagt
    10021 caccgagaaa cttgccgggg ccgaaaggct tgtcttcgcg gaacaaggac aaggtgcagc
    10081 cgtcaaggtt aaggctggcc atatcagcga ctgaaaagcg gccagcctcg gccttgtttg
    10141 acgtataacc aaagccaccg ggcaaccaat agcccttgtc acttttgatc aggtagaccg
    10201 accctgaagc gcttttttcg tattccataa aacccccttc tgtgcgtgag tactcatagt
    10261 ataacaggcg tgagtaccaa cgcaagcact acatgctgaa atctggcccg cccctgtcca
    10321 tgcctcgctg gcggggtgcc ggtgcccgtg ccagctcggc ccgcgcaagc tggacgctgg
    10381 gcagacccat gaccttgctg acggtgcgct cgatgtaatc cgcttcgtgg ccgggcttgc
    10441 gctctgccag cgctgggctg gcctcggcca tggccttgcc gatttcctcg gcactgcggc
    10501 cccggctggc cagcttctgc gcggcgataa agtcgcactt gctgaggtca tcaccgaagc
    10561 gcttgaccag cccggccatc tcgctgcggt actcgtccag cgccgtgcgc cggtggcggc
    10621 taagctgccg ctcgggcagt tcgaggctgg ccagcctgcg ggccttctcc tgctgccgct
    10681 gggcctgctc gatctgctgg ccagcctgct gcaccagcgc cgggccagcg gtggcggtct
    10741 tgcccttgga ttcacgcagc agcacccacg gctgataacc ggcgcgggtg gtgtgcttgt
    10801 ccttgcggtt ggtgaagccc gccaagcggc catagtggcg gctgtcggcg ctggccgggt
    10861 cggcgtcgta ctcgctggcc agcgtccggg caatctgccc ccgaagttca ccgcctgcgg
    10921 cgtcggccac cttgacccat gcctgatagt tcttcgggct ggtttccact accagggcag
    10981 gctcccggcc ctcggctttc atgtcatcca ggtcaaactc gctgaggtcg tccaccagca
    11041 ccagaccatg ccgctcctgc tcggcgggcc tgatatacac gtcattgccc tgggcattca
    11101 tccgcttgag ccatggcgtg ttctggagca cttcggcggc tgaccattcc cggttcatca
    11161 tctggccggt ggtggcgtcc ctgacgccga tatcgaagcg ctcacagccc atggccttga
    11221 gctgtcggcc tatggcctgc aaagtcctgt cgttcttcat cgggccacca agcgcagcca
    11281 gatcgagccg tcctcggttg tcagtggcgt caggtcgagc aagagcaacg atgcgatcag
    11341 cagcaccacc gtaggcatca tggaagccag catcacggtt agccatagct tccagtgcca
    11401 cccccgcgac gcgctccggg cgctctgcgc ggcgctgctc acctcggcgg ctacctcccg
    11461 caactctttg gccagctcca cccatgccgc ccctgtctgg cgctgggctt tcagccactc
    11521 cgccgcctgc gcctcgctgg cctgctgggt ctggctcatg acctgccggg cttcgtcggc
    11581 cagtgtcgcc atgctctggg ccagcggttc gatctgctcc gctaactcgt tgatgcctct
    11641 ggatttcttc actctgtcga ttgcgttcat ggtctattgc ctcccggtat tcctgtaagt
    11701 cgatgatctg ggtgttggcg gtgtcgatgt tcagggccac gtctgcccgg tcggtgcgga
    11761 tgccccggcc ttccatctcc accacgttcg gccccaggtg aacaccgggc aggcgctcga
    11821 tgccctgcgc ctcaagtgtt ctgtggtcaa tgcgggcgtc gtggccagcc cgctctaatg
    11881 cccggttggc atggtcggcc catgcctcgc gggtctgctc aagccatgcc ttgggcttga
    11941 gcgcttcggt cttctgtgcc ccgcccttct ccggggtctt gccgttgtac cgcttgaacc
    12001 actgagcggc gggccgctcg atgccgtcat tgatccgctc ggagatcatc aggtggcagt
    12061 gcgggttctc gccgccaccg gcatggatgg ccagcgtata cggcaggcgc tcggcaccgg
    12121 tcaggtgctg ggcgaactcg gacgccagcg ccttctgctg gtcgagggtc agctcgaccg
    12181 gcagggcaaa ttcgacctcc ttgaacagcc gcccattggc gcgttcatac aggtcggcag
    12241 catcccagta gtcggcgggc cgctcgacga actccggcat gtgcccggat tcggcgtgca
    12301 agacttcatc catgtcgcgg gcatacttgc cttcgcgctg gatgtagtcg gccttggccc
    12361 tggccgattg gccgcccgac ctgctgccgg ttttcgccgt aaggtgataa atcgccatgc
    12421 tgcctcgctg ttgcttttgc ttttcggctc catgcaatgg ccctcggaga gcgcaccgcc
    12481 cgaagggtgg ccgttaggcc agtttctcga agagaaaccg gtaagtgcgc cctcccctac
    12541 aaagtagggt cgggattgcc gccgctgtgc ctccatgata gcctacgaga cagcacatta
    12601 acaatggggt gtcaagatgg ttaaggggag caacaaggcg gcggatcggc tggccaagct
    12661 cgaagaacaa cgagcgcgaa tcaatgccga aattcagcgg gtgcgggcaa gggaacagca
    12721 gcaagagcgc aagaacgaaa caaggcgcaa ggtgctggtg ggggccatga ttttggccaa
    12781 ggtgaacagc agcgagtggc cggaggatcg gctcatggcg gcaatggatg cgtaccttga
    12841 acgcgaccac gaccgcgcct tgttcggtct gccgccacgc cagaaggatg agccgggctg
    12901 aatgatcgac cgagacaggc cctgcggggc tgcacacgcg cccccaccct tcgggtaggg
    12961 ggaaaggccg ctaaagcggc taaaagcgct ccagcgtatt tctgcggggt ttggtgtggg
    13021 gtttagcggg ctttgcccgc ctttccccct gccgcgcagc ggtggggcgg tgtgtagcct
    13081 agcgcagcga atagaccagc tatccggcct ctggccgggc atattgggca agggcagcag
    13141 cgccccacaa gggcgctgat aaccgcgcct agtggattat tcttagataa tcatggatgg
    13201 atttttccaa caccccgcca gcccccgccc ctgctgggtt tgcaggtttg ggggcgtgac
    13261 agttattgca ggggttcgtg acagttattg caggggggcg tgacagttat tgcaggggtt
    13321 cgtgacagtt agtacgggag tgacgggcac tggc
//
