LOCUS       AP022237                2442 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W18-CRE-04_9 DNA, complete genome,
            strain: WP8-W18-CRE-04.
ACCESSION   AP022237
VERSION     AP022237.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194608
            Sequence Read Archive: DRR199444
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W18-CRE-04 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 400x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W18-CRE-04"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W18-CRE-04_9"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2018-02-13"
     gene            101..604
                     /locus_tag="WP8W18C04_P90010"
     CDS             101..604
                     /locus_tag="WP8W18C04_P90010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47469.1"
                     /translation="MAKRGAGVNTKKISRLERHTYMDENGEVKQDSKIETFSIPAEPPY
                     VKLYIADIAMIHGLSKSQNDVLLQLAMLIGWDGFVSVSKTRFEKTIQPRVHIEYQTFKN
                     IIAALVDKQIFFRAGRGELEANPFLFARGDWPSIYERRKKIELKISYDTNGRTVQTKVM
                     DDEC"
                     /name="hypothetical protein"
     gene            complement(891..1859)
                     /locus_tag="WP8W18C04_P90020"
     CDS             complement(891..1859)
                     /locus_tag="WP8W18C04_P90020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47470.1"
                     /translation="MANYAIMRCKKLTGMGSVASALQHCYRERETPNANAERTPENYCS
                     VSKSTDQAMGRVRELLPEKRRKDAVLAVEYVMTASPEWWKEATPRQQAEFFARSEQWLE
                     KKYGKDRVVAAVVHRDEATPHLSAFVVPLTQDGRLSAKEFIGGRSKMREDQSTYAESVK
                     KLGLERGIEGSRATHQTVQHYYESINRGTRSQVSISPEALEPRVLRKGIFTKDVEDQAA
                     IAKRLSHAVNDGFAGTIAMASQSAQNAKRARELQKTMDAQQKRLQSVTEPFKGLSREQM
                     TEILMMAQRFKQQNQEKEKQQRIEREKQRQTRSRGMGGMER"
                     /name="hypothetical protein"
     Typing          complement(895..1868)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.077"
                     /coverage="96.0"
                     /evalue="0.0"
ORIGIN
        1 cacctcaagg actaatcgta ctcacctcaa gtactaatcg tacttgatac aaaggacttg
       61 actgtatgcc tctttagtac ttaaatgcga gtactaaatc atggcgaaac gtggggctgg
      121 agtgaatacg aaaaagattt ctaggcttga acggcataca tacatggatg aaaacggtga
      181 ggtaaaacaa gactctaaga tcgagacctt ttccatccca gccgaacctc cctacgtcaa
      241 gctatacatt gcagacattg cgatgattca tggtttatca aaatctcaaa atgatgtgct
      301 tttacaattg gcaatgctga ttggatggga tgggtttgtg tctgtatcaa aaactagatt
      361 tgaaaaaacc atacagccca gggttcatat tgaatatcag acctttaaaa acataattgc
      421 ggctcttgta gataaacaaa tattcttccg ggcagggcgt ggggaacttg aagctaaccc
      481 ctttcttttt gcacggggag actggccatc aatttacgaa agaagaaaga aaattgagtt
      541 aaagatcagt tatgacacca atggaagaac ggtgcagacg aaggtaatgg atgatgaatg
      601 ctgaagataa actttttaaa aatgtagaca ggctaatgct gcatgattca ctaaaaaaga
      661 atgaggttct gactgtttgg gagcgtactt ttttaagcag tgtttacagc atatatcgta
      721 gaactagcgg taataaatac tcaactaagg gtttgtcacc aaagcagaaa aacgcagctt
      781 tttctatttt aaaaaaacat aattaccatc tgaatgatta attttttcgt ggtgaatgcc
      841 gcaaatgacg tcactttgcg gcgttagccc tatcctgcat cgcatcaaaa ctaacgctcc
      901 ataccaccca tgccacgcga tcgcgtctgt cgctgttttt cacgttcaat acgctgctgt
      961 ttttcctttt cctggttttg ctgcttaaac cgctgagcca tcatcaggat ttcggtcatc
     1021 tgctctctgg aaagcccctt aaaaggctct gtgacgctct gtaggcgttt ctgctgcgca
     1081 tccatagtct tctgtagctc acgcgctctt tttgcgttct gggcgctctg agaggccata
     1141 gcgatggttc ctgcaaagcc atcgttcact gcgtggctta gacgtttggc tatggctgcc
     1201 tgatcttcca cgtctttggt gaaaatgccc ttccgcaata cacgcggctc cagcgcctcc
     1261 ggcgagatcg atacctggct acgggtgcca cggtttatcg attcgtagta gtgctgaact
     1321 gtctgatgcg tggcgcggct tccctcgatg ccgcgctcaa gacccagttt ttttactgat
     1381 tcagcgtagg tgctctgatc ttcacgcatc tttgaccgcc cgccaataaa ctctttcgcg
     1441 gacagccgtc catcctgcgt caatggcacc acgaacgcag acaggtgcgg ggtggcctcg
     1501 tcccggtgaa ccaccgccgc cacaacacga tccttcccgt atttcttctc cagccattgc
     1561 tcagagcggg cgaaaaactc cgcctgctgc cgtggtgtgg cctctttcca ccactcaggg
     1621 cttgctgtca taacgtattc aacggccagt accgcatctt tacggcgttt ttccggcagc
     1681 agctcccgca cacgccccat tgcctgatcc gttgatttgg acacagagca gtagttttct
     1741 ggtgtccgtt cagcgttagc gttcggcgtc tcacgctctc ggtagcagtg ctgcaacgcg
     1801 ctggccacgc ttcccattcc cgtcagcttt ttgcagcgca ttatcgcgta gttcgccatg
     1861 ttcccccctg attccggcat tcattcagcc aggcggggga gcgcattgcg atacctccgg
     1921 cgggccacta tcaagatgat agtgtactca ctagactttg ctacgcaaaa tcgttcgggc
     1981 agcaagctgc cagaaggtca aaatcaacgg cggtctggca ggtggaaggc gtcgcttcgc
     2041 gccacccccc accactgcca agcctgtttt atccggagtg tcgtcaaggg taaatggcac
     2101 tcgctacgct cgcccttgac gaaatcaggc aatgaaatcg tctttgaaag agcaggaacc
     2161 gctcgccgca gctttggctg aggcgtaccg caggagcaac gcgacgagga acgaacagcc
     2221 aaagcgagga agcggaattt ccactccccg tacatagaaa tgcgtacact ccttgctaaa
     2281 aaaaggcagt ttttcagtgt ctaaaaatgc cgtttttcag cccttatttt cccccttaat
     2341 atatagatat agatatggga acccgcttgt accaagggct agagcgaaga atcgtactca
     2401 cctcaagtac caatcgtact cacctcaagt actaatcgta ct
//
