LOCUS       AP022236                4165 bp    DNA     circular BCT 22-JUL-2020
DEFINITION  Enterobacter cloacae plasmid pWP8-W18-CRE-04_8 DNA, complete genome,
            strain: WP8-W18-CRE-04.
ACCESSION   AP022236
VERSION     AP022236.1
DBLINK      BioProject: PRJDB6962
            BioSample: SAMD00194608
            Sequence Read Archive: DRR199444
KEYWORDS    .
SOURCE      Enterobacter cloacae
  ORGANISM  Enterobacter cloacae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     complete genome sequences of Enterobacter cloacae str.
            WP8-W18-CRE-04 isolated from wastewater treatment plant effluent
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Sekizuka,T., Itokawa,K., Yatsu,K., Inamine,Y. and Kuroda,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-DEC-2019) Contact:Tsuyoshi Sekizuka National Institute
            of Infectious Diseases, Pathogen Genomics Center; 1-23-1 Toyama,
            Shinjuku-ku, Tokyo 162-8640, Japan
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: flye v2.5; minimap v0.2-r124; racon v1.1.0;
            circlator v1.5.3; Pilon v1.18; SPAdes v3.11.1; A5-miseq v20140604
            Genome Coverage       :: 400x
            Sequencing Technology :: Sequel; NextSeq 500
            ##Genome-Assembly-Data-END##
            Annotated by DFAST v.1.2.3 (https://dfast.nig.ac.jp).
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Enterobacter cloacae"
                     /mol_type="genomic DNA"
                     /strain="WP8-W18-CRE-04"
                     /isolation_source="wastewater treatment plant effluent"
                     /db_xref="taxon:550"
                     /plasmid="pWP8-W18-CRE-04_8"
                     /geo_loc_name="Japan:Tokyo"
                     /lat_lon="35.6316 N 139.7460 E"
                     /collection_date="2018-02-13"
     gene            101..1036
                     /locus_tag="WP8W18C04_P80010"
     CDS             101..1036
                     /locus_tag="WP8W18C04_P80010"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47465.1"
                     /translation="MTKPPLDLFNDRLPNKPYFSDDLHFGVRIAGKERAILAKYIQFNQ
                     PHAMFWLGFDVDRTGAAIDWSDRNAPAPTLTITNPENGHAHLLYALETPVRTAPDGKMK
                     PLRYAAAVENALRKKLEADEGYSGLICKNPNHGHWKIAVWQPELYTLDWLADSLDLSAA
                     NDKEIVADYGLGRNCTLFDKTRKWAYRAIRQGWPEYEQWLQACYERASAYNLQFSSPLD
                     ENEVNGIAKSIAKWTHSNFDEASFKKYVEDTHSSYIQSLRGKKSRGGGRKKMPEDKILA
                     IKEMVSEGMSYREISKLENVSIGFISKHCK"
                     /name="hypothetical protein"
     gene            1053..1703
                     /locus_tag="WP8W18C04_P80020"
     CDS             1053..1703
                     /locus_tag="WP8W18C04_P80020"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47466.1"
                     /translation="MRYVKISLSVATFLLFWYVSSFFTGCSDGWGSSSIGLRGACSYHG
                     GVSKLPIIIIFLGLIASFFVFFLFPDSESATSKKDTFPVVNKESNESIPKIKTNTHNEI
                     KVKRELKIEIENLNDKKFSKIFIYSDGLFIKEFNPENKMKVSTKRINKNKILKISNLLR
                     EGKGGAINPILDVEAKGYLVRLFENHAHESKTYFIDRSEKQAFSDIKELINSV"
                     /name="hypothetical protein"
     gene            2081..2272
                     /locus_tag="WP8W18C04_P80030"
     CDS             2081..2272
                     /locus_tag="WP8W18C04_P80030"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47467.1"
                     /translation="MNKQQKTAVNMAKFIQAQSLLLLDRLNDLDLDHEADFCEKLHEDA
                     ERLYRSLSARLNEQQDGA"
                     /name="hypothetical protein"
     gene            2744..2935
                     /locus_tag="WP8W18C04_P80040"
     CDS             2744..2935
                     /locus_tag="WP8W18C04_P80040"
                     /inference="COORDINATES: ab initio
                     prediction:Prodigal:2.6.3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="BBT47468.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELLDGN"
                     /name="hypothetical protein"
ORIGIN
        1 gcggaagatc acgataacga gggcgcagga ctaaattcct aagaaggata ctagcacatg
       61 cacccagcaa gacaacagcc cgccataagt tcaggcgtga gtgactaagc cccccctcga
      121 tctttttaac gaccgtctcc caaacaaacc gtacttctcg gacgatttgc acttcggcgt
      181 gcgcatcgcc gggaaagagc gcgcgatcct ggcgaaatac atccagttca accagccgca
      241 cgccatgttc tggctgggat tcgacgtcga tcgcaccggt gcggccatcg actggagcga
      301 ccggaacgcg cctgcgccca cgctgaccat caccaacccg gaaaacggac atgcgcacct
      361 gctgtacgcg ctggaaaccc cggtacgcac cgctcctgac ggaaaaatga agccgctgcg
      421 ctacgccgcc gccgtggaga acgcgctgcg taaaaaatta gaggctgacg aggggtattc
      481 gggactaatc tgcaaaaatc cgaaccacgg ccactggaaa atcgccgtgt ggcagccgga
      541 actctacacg ctggactggc tggccgactc gctcgacctg agtgccgcga acgacaaaga
      601 aatcgtcgcc gactatggtt taggccgtaa ctgcactctg ttcgataaaa cccgcaagtg
      661 ggcataccgg gcgatccgcc agggctggcc ggagtatgag cagtggctgc aagcctgcta
      721 tgaacgtgcc agtgcctaca atctgcaatt ttcgagccct cttgatgaaa atgaggtgaa
      781 cggtattgcg aaaagtattg ctaaatggac tcatagtaat tttgatgaag cttcatttaa
      841 gaagtatgtg gaagatacac acagtagtta cattcaatca ttaagaggaa aaaagagtcg
      901 tggagggggg cggaagaaaa tgccagaaga taagattctt gcaataaaag agatggtttc
      961 tgaaggcatg tcttatagag agatctccaa attagaaaat gtatccatag ggtttattag
     1021 caaacattgc aaatgaaata aattggtgat ttatgcgtta cgtaaagatt tccctttcag
     1081 tagctacttt tttattgttt tggtatgtct ctagcttttt cacagggtgt tctgatggct
     1141 ggggttcttc atccataggg ttgagaggag cgtgttcata tcatggcggc gttagtaagc
     1201 tacctataat cattatcttt ttgggcctca tcgctagttt ttttgttttt tttcttttcc
     1261 ctgatagtga aagtgctact agcaaaaagg atactttccc tgttgtaaac aaggaaagta
     1321 atgagtctat tccaaaaata aaaaccaata cgcataacga aataaaagtt aagcgcgaat
     1381 tgaaaataga aatagagaat ttaaacgata agaaattctc taaaatattt atttattctg
     1441 atggtctttt tattaaagag ttcaatcctg aaaataaaat gaaggtgagt accaaaagga
     1501 ttaataaaaa taaaatatta aaaataagta atcttcttcg tgaagggaag gggggggcta
     1561 taaatcccat tctcgatgtg gaagcaaaag ggtatcttgt gcgtttgttc gaaaatcatg
     1621 cgcatgaaag taaaacttat tttattgata gatcggagaa acaagccttt tcggacatta
     1681 aagaactcat caatagcgtc taatgggaag gttgtttatg aaccctttta tcgaaggtgt
     1741 tcataaacaa cctatatcag ataacagccc tctctagcag agggcttttt tttgcagcca
     1801 gcacagaacc tgacatactg cactaccctt taagccagta cacacggagt cccctctggc
     1861 gagggtccac cgtgactggt tcaggggatt gcatcccccg aaacccccgt gagaatggtg
     1921 cgaatctttt gcgaattatt cccacattca ctgcgaatat tctcaccgtg attagccggt
     1981 aacgctcgcc gcagtctcat gaccgaacgg agtgagcgaa tgagcgagga agcggaaaac
     2041 catcagaacg tacagtaagc actgtcgcgc cggggccttc atgaacaaac aacaaaagac
     2101 agccgttaac atggctaaat tcatccaggc tcagtccctg cttcttctgg acaggcttaa
     2161 tgacctggat ctggatcatg aggctgattt ctgcgagaaa ctccatgagg atgcggaacg
     2221 cctttaccga agcctgtcag cgcgcctgaa tgagcagcag gacggtgcgt aatgtctccg
     2281 ttctaagtat taccggagta ataccggcat cgccttaatt ttcattgtgt tagcgcttaa
     2341 cattattacc ccggtaatac ccagaatccc tgtatttttt cttttttatt tatttttcat
     2401 gaagttgcga tgaaaatcgc agctgcgtta ggtgtatgac cattttgagg ttgaaaaata
     2461 atcaatcatg gcgggtgatt tacgcaggtt aagggtagat cgtgacaaaa aaagcgttcc
     2521 tcactgtatc gttcatgcct gcatgaacgg attatttttc cggactacag gtaaactaca
     2581 ggaagactac aaaagttgat gacttaatcc tgatttttag tgcgatgact acagataaac
     2641 tacaaaataa ctacaggatt acctcagatc cgttctctga accattcgta catccgaccc
     2701 ccttaccggt tttacactga gcactgatgc tctgaggtcc cttatgacga aacaacagaa
     2761 aacagcgctg aacatggcta aattcatcca gaatcagtct ctgctcctgc tggagaagct
     2821 taacgagctt gatctcgatg ctgaggccga cctttgtgag aaactccacg atgatgcgga
     2881 gcatcttttc cgtacactgt cctcgagact ggatgaatta ctggatggta actgatggcc
     2941 agagtgtcta ttagtgaagc agcacggctt gttaaggtta gtcgccctac tatttacaag
     3001 atgattaaca gtggtaagtt aagttacacc tctgttgtaa agcacgggaa gagtattaaa
     3061 gttattgata catcagagct tattagggtg tttggttctc ttgatggtgt catcgatgat
     3121 gtaaaaagtg acgcagaatc tacaggtatt aacagcgttg gtttacatga tttacaacat
     3181 agaatcgctt tacttgaagc tgaaaacgat ggtcttaaag gggctgttaa ggctcgagat
     3241 gagcatattg attcccttcg tcaggctatg cagcttctgg agcacaagca cgaaccttct
     3301 tcaccacccc attccccgtg gtggaaattc tggaaaaaat cctgatcccg ccatgcttga
     3361 gttttcctct ccggccatcc cggggcgttc ctgaaccttc ccaaaaatcg ccagatccgg
     3421 gaacactcct caccacgaaa aatacccaca caccacgcaa aattaaaatt ttgcagataa
     3481 ctccttatta tcagttggtt aggttttatt aaattacgca gtattacctt ctgtaaggtc
     3541 aatccaagtc gtcggtgatt cgtgtacgac ttaccgacga ctcgtcatag agtcatcgac
     3601 gactcactcc acactatcag taacttatga cgacttgact ctagagtcga atcgtcgctt
     3661 tggcgttttt ttgaccgtgg tgactctaga gtcaagtcac gagtcgaatc gccggtgaat
     3721 cgtgtggtga gtcgtccggc gtcttgccat aacgtgattg ccggccgtcg acgaacccga
     3781 ttcctgaacg tgctgaaaac cggttttccg gctgcgccgg caacgcttca cggccacgca
     3841 ataaattgcg tccccgttca ccgttttcgg gtaagaagaa catcggaaac aggacttacc
     3901 ccggctgaat ggcgtgaatt tccgcgctat gcacttgcgc gcatactcat gcatgccgta
     3961 aaaacagagc ctgcgcgttt ctggcgggtt ttcgggtggt ttgctgcctg ttttgccggt
     4021 ttcccgtcag aaacgcgctg agggcgcctg aggcggtgct gataacagat gttatggtaa
     4081 atcgggggga ggtcgacaag tgcctgaact gggtgtagtt ttaaccccag aaagcaaacg
     4141 cccccgaaat cgtgcaatca acttg
//
